Rh5AG437700

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000618
Physical Location & Seq
Reverse (-)
75195059 .. 75210926
15868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG437700.1

Sequence Viewer

Length: 354 bp
ATGAACTACTGGACACTATACTGGTTAAGCAGGCAAAAGAATACGCTTGCCTATTGCATCTTCAAAGGAATCAGTTGCTTTAGGTCAATTTTAAGTCATCGCAAGAGGTGGTCTGAATCCATGGCACAATTTAGTCTCATTGACTTTTGTGTAAAAGAGAAACCCCTCAAGCGGCATGGATTTCTAAGGCTCTTTGGTATCTTCGATACGTATGAAAAGTATCGACATGCGTCTTTTGCAAAAGTCCATGTCAGAATGAAAGAGCTTATCTTTCAGAAACTTCAAGATGCTAGAAATCATGTTAATGAAGAAAGAGGTCCTGAATCAAAATATGATCAAATTAGGGATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

117

Amino Acids

14.26

Weight (kDa)

9.78

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 2 cut(s) 64, 284
AluBI AGCT 1 cut(s) 265
AluI AGCT 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 317
AvaII GGWCC 1 cut(s) 317
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 2 cut(s) 291, 352
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 317
BmsI GCATC 2 cut(s) 66, 277
BoxI GACNNNNGTC 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 14, 26
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 171
BseNI ACTGG 2 cut(s) 14, 26
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 140
Bsp143I GATC 1 cut(s) 334
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 14, 26
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 334
BssT1I CCWWGG 1 cut(s) 120
BstBAI YACGTR 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 32, 48
BstDEI CTNAG 1 cut(s) 185
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 230
BstPAI GACNNNNGTC 1 cut(s) 229
BstSNI TACGTA 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 32, 48
Cfr13I GGNCC 1 cut(s) 317
CseI GACGC 1 cut(s) 219
CviAII CATG 5 cut(s) 121, 176, 227, 248, 299
CviJI RGCY 2 cut(s) 190, 265
CviKI_1 RGCY 2 cut(s) 190, 265
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
Eco105I TACGTA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 5 cut(s) 124, 179, 230, 251, 302
FaiI YATR 8 cut(s) 19, 122, 177, 213, 228, 249, 300, 333
FatI CATG 5 cut(s) 120, 175, 226, 247, 298
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 173
FspBI CTAG 2 cut(s) 291, 352
GluI GCNGC 1 cut(s) 173
HgaI GACGC 1 cut(s) 219
Hin1II CATG 5 cut(s) 124, 179, 230, 251, 302
HinfI GANTC 3 cut(s) 69, 116, 323
Hpy188I TCNGA 3 cut(s) 115, 254, 276
Hpy188III TCNNGA 2 cut(s) 284, 320
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 57, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 5 cut(s) 124, 179, 230, 251, 302
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 1 cut(s) 334
LpnPI CCDG 3 cut(s) 7, 16, 333
LweI GCATC 2 cut(s) 66, 277
MaeI CTAG 2 cut(s) 291, 352
MaeII ACGT 1 cut(s) 209
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 3 cut(s) 52, 193, 320
MluCI AATT 3 cut(s) 87, 128, 339
MnlI CCTC 3 cut(s) 99, 176, 308
MseI TTAA 3 cut(s) 26, 92, 303
MslI CAYNNNNRTG 1 cut(s) 303
MwoI GCNNNNNNNGC 1 cut(s) 236
NcoI CCATGG 1 cut(s) 120
NdeII GATC 1 cut(s) 334
NlaIII CATG 5 cut(s) 124, 179, 230, 251, 302
NspI RCATGY 1 cut(s) 230
PfeI GAWTC 3 cut(s) 69, 116, 323
PkrI GCNGC 1 cut(s) 174
Ppu21I YACGTR 1 cut(s) 210
PpuMI RGGWCCY 1 cut(s) 317
PshAI GACNNNNGTC 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 317
PspPI GGNCC 1 cut(s) 317
PspPPI RGGWCCY 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 3 cut(s) 26, 92, 303
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 1 cut(s) 317
SetI ASST 5 cut(s) 86, 110, 212, 267, 319
SfaNI GCATC 2 cut(s) 66, 277
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SnaBI TACGTA 1 cut(s) 210
Sse9I AATT 3 cut(s) 87, 128, 339
SsiI CCGC 1 cut(s) 172
SspMI CTAG 2 cut(s) 291, 352
StyI CCWWGG 1 cut(s) 120
TaiI ACGT 1 cut(s) 212
TaqI TCGA 2 cut(s) 204, 223
TasI AATT 3 cut(s) 87, 128, 339
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 3 cut(s) 69, 116, 323
Tru1I TTAA 3 cut(s) 26, 92, 303
Tru9I TTAA 3 cut(s) 26, 92, 303
TspDTI ATGAA 4 cut(s) 17, 228, 272, 321
VpaK11BI GGWCC 1 cut(s) 317
XceI RCATGY 1 cut(s) 230
XspI CTAG 2 cut(s) 291, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.