Rmu_co8480723.1_g000001

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8480723.1
OG0000618
Physical Location & Seq
Reverse (-)
2 .. 1654
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8480723.1_g000001.1.cds

Sequence Viewer

Length: 1653 bp
atgtgggaaatccgtgtcactgtaatagtgagccttatcttacaaggcattctcattgtgttaggcagcaggagaaagcattcaaccagcaataccctcagattcattttgtgggttgcctacttgtctgcagactgggttgcaacagtttcgctgagtgcactttctagcaataatgctacaaaccctgcaagtaattctatagtccctgaccaaataattacagcattctgggctccatttcttctccttcatcttggtggcccggacaccattgctgcttcgtctttagaagacaatgagctgtggtggagacactttcttctactagttgtgcaggtgggagtggctatttatgtcttgcttagagcctgggcgggtcatgtgcttaattttctagccattccaatgtttgttgctggttttatcaagtttgcagaaaagacttgggttctgagatctgcaagcagtgaacattttagggaatcaatgttccccaaacctgatccaggccctaactatgcaagatacatgaatgagttccgctcaaagaaagaggaagggtttaaggtcgagttccacattgaagatcccaaagtgggctatcattcctatacagcaccagaaggtgtcttttcaagttcagccaatttgcagaatgcccatgttctttttttctacaatattttcaaggggctatttgctgatctcatcctcaacattgatgatgttgcaaaaggtatatctttcttccagagcagatcctgtgatgaagcttttaaagtgatagagattgagcttggctttatgtatgatatgttatatacaaagtcaatgctgatttattctgtcatgggtgccattcttcgattcatcagcttcttttgcaccatctctgtttctgtggtcttcttgatcatcaacaagcaagattactcggaacaaaatgtactgatcacttttatattgctgggtggagctgtcagcctagagatctatgctgtaattttactatttttatcagactggacagtgctgtggctgagtaagaacacgaaagcaatttcatgcgtttcattacttgaaagcaagaggtggtctaatgtcattatacaagacaatttaataaatcattgtctcgaagatcagccttcacggtgcagatttctccacaaggtccccacatataaaaatttgaagaagcatctctacaggaattcaaggccagttgaagaagagttgaaggattttatatttcaccagcttctaaagaaatcaaggagtgccactaaattcgaggatctgaacaaaagaaatgtcttgataaagtcttggggtgaatgggttcttgaacaagagaaatgtcttgatacatttggatggagcattgaagaggagttcgataaaagcattcttctttggcatattgccacagacctctgttattactctgatctcgaaaaagaccaaaactttgttaaggataagtactgttccgacagcaggtcgttatcaaactatatgttgtatcttctagtcatgtgtcccttcatgctacccaatgggatcggaaaaatcaggttccaagacacgtgtgcggaagccactgaattcttcgaagaaagaaaatgcaaaggaggtggtaacgctagc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

551

Amino Acids

63.31

Weight (kDa)

6.52

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 2 cut(s) 328, 1494
AccB1I GGYRCC 1 cut(s) 857
AccBSI CCGCTC 1 cut(s) 546
AciI CCGC 3 cut(s) 377, 544, 1598
AclWI GGATC 5 cut(s) 500, 584, 756, 1308, 1574
AcsI RAATTY 4 cut(s) 1192, 1216, 1292, 1610
AcvI CACGTG 1 cut(s) 1593
AfaI GTAC 2 cut(s) 951, 1490
AfiI CCNNNNNNNGG 3 cut(s) 509, 599, 1503
AflIII ACRYGT 2 cut(s) 1590, 1592
AhdI GACNNNNNGTC 1 cut(s) 1504
AhlI ACTAGT 1 cut(s) 328
AjnI CCWGG 2 cut(s) 371, 508
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 6 cut(s) 304, 776, 799, 879, 980, 1264
AluI AGCT 6 cut(s) 304, 776, 799, 879, 980, 1264
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 307, 1142
Alw44I GTGCAC 1 cut(s) 159
AlwI GGATC 5 cut(s) 500, 584, 756, 1308, 1574
AlwNI CAGNNNCTG 1 cut(s) 765
AoxI GGCC 3 cut(s) 262, 511, 1223
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 66, 278
ApoI RAATTY 4 cut(s) 1192, 1216, 1292, 1610
ArsI GACNNNNNNTTYG 4 cut(s) 1302, 1334, 1457, 1489
Asp700I GAANNNNTTC 3 cut(s) 79, 539, 1344
AspS9I GGNCC 3 cut(s) 263, 512, 1177
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 2 cut(s) 1250, 1349
AsuII TTCGAA 1 cut(s) 1617
AsuNHI GCTAGC 1 cut(s) 1649
AvaII GGWCC 1 cut(s) 1177
BaeGI GKGCMC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 238
BarI GAAGNNNNNNTAC 2 cut(s) 1193, 1225
BbrPI CACGTG 1 cut(s) 1593
BbsI GAAGAC 2 cut(s) 300, 901
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 78, 265
BccI CCATC 2 cut(s) 899, 1374
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BciT130I CCWGG 2 cut(s) 373, 510
BclI TGATCA 2 cut(s) 915, 954
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 307, 1142
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 6 cut(s) 168, 329, 398, 989, 1535, 1650
BfmI CTRYAG 3 cut(s) 129, 201, 1210
BfuAI ACCTGC 2 cut(s) 328, 1494
BglII AGATCT 2 cut(s) 458, 993
BisI GCNGC 2 cut(s) 67, 279
BlsI GCNGC 2 cut(s) 68, 280
BmcAI AGTACT 1 cut(s) 1490
Bme1390I CCNGG 3 cut(s) 266, 373, 510
Bme18I GGWCC 1 cut(s) 1177
BmeRI GACNNNNNGTC 1 cut(s) 1504
BmgT120I GGNCC 3 cut(s) 263, 512, 1177
BmiI GGNNCC 4 cut(s) 237, 859, 1179, 1583
BmrFI CCNGG 3 cut(s) 266, 373, 510
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 1213
BmtI GCTAGC 1 cut(s) 1653
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 2 cut(s) 300, 901
BplI GAGNNNNNCTC 2 cut(s) 530, 562
Bpu14I TTCGAA 1 cut(s) 1617
BpuMI CCSGG 1 cut(s) 266
BsaAI YACGTR 1 cut(s) 1593
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 509, 599, 1503
Bse1I ACTGG 3 cut(s) 140, 1031, 1226
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 373, 510
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 711, 1385
BseLI CCNNNNNNNGG 3 cut(s) 509, 599, 1503
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 4 cut(s) 112, 146, 446, 1034
BseNI ACTGG 3 cut(s) 140, 1031, 1226
BseRI GAGGAG 1 cut(s) 1409
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 2 cut(s) 78, 265
BseYI CCCAGC 1 cut(s) 970
BsgI GTGCAG 2 cut(s) 356, 1180
BshFI GGCC 3 cut(s) 264, 513, 1225
BshNI GGYRCC 1 cut(s) 857
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 3 cut(s) 191, 1163, 1530
BslI CCNNNNNNNGG 3 cut(s) 509, 599, 1503
BsmAI GTCTC 2 cut(s) 307, 1142
BsmFI GGGAC 3 cut(s) 191, 1163, 1530
BsmI GAATGC 5 cut(s) 48, 79, 227, 664, 1410
BsnI GGCC 3 cut(s) 264, 513, 1225
Bsp119I TTCGAA 1 cut(s) 1617
Bsp1286I GDGCHC 2 cut(s) 163, 238
BspACI CCGC 3 cut(s) 377, 544, 1598
BspANI GGCC 3 cut(s) 264, 513, 1225
BspCNI CTCAG 4 cut(s) 111, 147, 447, 1035
BspLI GGNNCC 4 cut(s) 237, 859, 1179, 1583
BspMAI CTGCAG 1 cut(s) 133
BspMI ACCTGC 2 cut(s) 328, 1494
BspOI GCTAGC 1 cut(s) 1653
BspPI GGATC 5 cut(s) 500, 584, 756, 1308, 1574
BspT104I TTCGAA 1 cut(s) 1617
BspT107I GGYRCC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 3 cut(s) 140, 1031, 1226
BssECI CCNNGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 373, 510
Bst4CI ACNGT 5 cut(s) 22, 148, 1033, 1158, 1493
Bst6I CTCTTC 2 cut(s) 1230, 1386
BstBAI YACGTR 1 cut(s) 1593
BstBI TTCGAA 1 cut(s) 1617
BstC8I GCNNGC 2 cut(s) 466, 1651
BstDEI CTNAG 5 cut(s) 98, 155, 365, 455, 1043
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 711, 1385
BstMAI GTCTC 2 cut(s) 307, 1142
BstMWI GCNNNNNNNGC 2 cut(s) 233, 885
BstNI CCWGG 2 cut(s) 373, 510
BstSCI CCNGG 3 cut(s) 264, 371, 508
BstSFI CTRYAG 3 cut(s) 129, 201, 1210
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 78, 265
BstV2I GAAGAC 2 cut(s) 300, 901
BstX2I RGATCY 5 cut(s) 458, 589, 761, 993, 1300
BstYI RGATCY 5 cut(s) 458, 589, 761, 993, 1300
BsuRI GGCC 3 cut(s) 264, 513, 1225
BtsCI GGATG 2 cut(s) 711, 1385
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 4 cut(s) 18, 475, 1038, 1605
BveI ACCTGC 2 cut(s) 328, 1494
Cac8I GCNNGC 2 cut(s) 466, 1651
CaiI CAGNNNCTG 1 cut(s) 765
Cfr13I GGNCC 3 cut(s) 263, 512, 1177
Csp6I GTAC 2 cut(s) 950, 1489
CspCI CAANNNNNGTGG 1 cut(s) 1621
CviAII CATG 7 cut(s) 383, 532, 665, 853, 1068, 1540, 1552
CviQI GTAC 2 cut(s) 950, 1489
DdeI CTNAG 5 cut(s) 98, 155, 365, 455, 1043
DraI TTTAAA 1 cut(s) 781
DriI GACNNNNNGTC 1 cut(s) 1504
Eam1104I CTCTTC 2 cut(s) 1230, 1386
Eam1105I GACNNNNNGTC 1 cut(s) 1504
EarI CTCTTC 2 cut(s) 1230, 1386
Eco24I GRGCYC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 1177
Eco72I CACGTG 1 cut(s) 1593
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 512, 1177
EcoRI GAATTC 2 cut(s) 1216, 1610
EcoRII CCWGG 2 cut(s) 371, 508
EcoT38I GRGCYC 1 cut(s) 238
FaeI CATG 7 cut(s) 386, 535, 668, 856, 1071, 1543, 1555
FaqI GGGAC 3 cut(s) 191, 1163, 1530
FatI CATG 7 cut(s) 382, 531, 664, 852, 1067, 1539, 1551
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 2 cut(s) 915, 954
Fnu4HI GCNGC 2 cut(s) 67, 279
FokI GGATG 2 cut(s) 698, 1392
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 2 cut(s) 67, 279
FspBI CTAG 6 cut(s) 168, 329, 398, 989, 1535, 1650
GluI GCNGC 2 cut(s) 67, 279
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 3 cut(s) 264, 513, 1225
HapII CCGG 1 cut(s) 266
Hin1II CATG 7 cut(s) 386, 535, 668, 856, 1071, 1543, 1555
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 3 cut(s) 102, 485, 870
HpaII CCGG 1 cut(s) 266
HphI GGTGA 2 cut(s) 1250, 1349
Hpy166II GTNNAC 2 cut(s) 161, 473
Hpy188I TCNGA 8 cut(s) 101, 456, 940, 1024, 1305, 1453, 1498, 1571
Hpy188III TCNNGA 7 cut(s) 754, 913, 1139, 1321, 1349, 1367, 1457
Hpy8I GTNNAC 2 cut(s) 161, 473
HpyAV CCTTC 6 cut(s) 260, 554, 620, 1161, 1237, 1558
HpyCH4III ACNGT 5 cut(s) 22, 148, 1033, 1158, 1493
HpyCH4IV ACGT 1 cut(s) 1592
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 885
HpyF3I CTNAG 5 cut(s) 98, 155, 365, 455, 1043
HpySE526I ACGT 1 cut(s) 1592
Hsp92II CATG 7 cut(s) 386, 535, 668, 856, 1071, 1543, 1555
Ksp22I TGATCA 2 cut(s) 915, 954
LmnI GCTCC 3 cut(s) 241, 977, 1383
Lsp1109I GCAGC 2 cut(s) 78, 265
LweI GCATC 1 cut(s) 1213
MaeI CTAG 6 cut(s) 168, 329, 398, 989, 1535, 1650
MaeII ACGT 1 cut(s) 1592
MaeIII GTNAC 2 cut(s) 16, 1643
MbiI CCGCTC 1 cut(s) 546
MflI RGATCY 5 cut(s) 458, 589, 761, 993, 1300
MhlI GDGCHC 2 cut(s) 163, 238
MmeI TCCRAC 1 cut(s) 1521
MnlI CCTC 8 cut(s) 107, 550, 725, 1086, 1291, 1387, 1448, 1631
MroXI GAANNNNTTC 3 cut(s) 79, 539, 1344
MseI TTAA 5 cut(s) 390, 567, 780, 1124, 1479
MslI CAYNNNNRTG 3 cut(s) 258, 407, 1378
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 266, 373, 510
Mva1269I GAATGC 5 cut(s) 48, 79, 227, 664, 1410
MvaI CCWGG 2 cut(s) 373, 510
MwoI GCNNNNNNNGC 2 cut(s) 233, 885
NciI CCSGG 1 cut(s) 266
NheI GCTAGC 1 cut(s) 1649
NlaIII CATG 7 cut(s) 386, 535, 668, 856, 1071, 1543, 1555
NlaIV GGNNCC 4 cut(s) 237, 859, 1179, 1583
NmuCI GTSAC 1 cut(s) 16
NspV TTCGAA 1 cut(s) 1617
PaqCI CACCTGC 1 cut(s) 328
PctI GAATGC 5 cut(s) 48, 79, 227, 664, 1410
PdmI GAANNNNTTC 3 cut(s) 79, 539, 1344
PfeI GAWTC 3 cut(s) 102, 485, 870
PkrI GCNGC 2 cut(s) 68, 280
PmaCI CACGTG 1 cut(s) 1593
PmlI CACGTG 1 cut(s) 1593
Ppu21I YACGTR 1 cut(s) 1593
PpuMI RGGWCCY 1 cut(s) 1177
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 2 cut(s) 371, 508
PspCI CACGTG 1 cut(s) 1593
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 2 cut(s) 371, 508
PspN4I GGNNCC 4 cut(s) 237, 859, 1179, 1583
PspPI GGNCC 3 cut(s) 263, 512, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PsrI GAACNNNNNNTAC 2 cut(s) 933, 965
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 5 cut(s) 458, 589, 761, 993, 1300
RsaI GTAC 2 cut(s) 951, 1490
RsaNI GTAC 2 cut(s) 950, 1489
RseI CAYNNNNRTG 3 cut(s) 258, 407, 1378
SaqAI TTAA 5 cut(s) 390, 567, 780, 1124, 1479
SatI GCNGC 2 cut(s) 67, 279
Sau96I GGNCC 3 cut(s) 263, 512, 1177
ScaI AGTACT 1 cut(s) 1490
ScrFI CCNGG 3 cut(s) 266, 373, 510
SduI GDGCHC 2 cut(s) 163, 238
SfaNI GCATC 1 cut(s) 1213
SfcI CTRYAG 3 cut(s) 129, 201, 1210
SfuI TTCGAA 1 cut(s) 1617
SinI GGWCC 1 cut(s) 1177
SmiMI CAYNNNNRTG 3 cut(s) 258, 407, 1378
SpeI ACTAGT 1 cut(s) 328
SsiI CCGC 3 cut(s) 377, 544, 1598
SspI AATATT 1 cut(s) 685
SspMI CTAG 6 cut(s) 168, 329, 398, 989, 1535, 1650
StyD4I CCNGG 3 cut(s) 264, 371, 508
TaaI ACNGT 5 cut(s) 22, 148, 1033, 1158, 1493
TaiI ACGT 1 cut(s) 1595
TaqI TCGA 7 cut(s) 573, 868, 1140, 1296, 1401, 1458, 1617
TatI WGTACW 2 cut(s) 949, 1488
TfiI GAWTC 3 cut(s) 102, 485, 870
Tru1I TTAA 5 cut(s) 390, 567, 780, 1124, 1479
Tru9I TTAA 5 cut(s) 390, 567, 780, 1124, 1479
TscAI CASTG 4 cut(s) 25, 475, 1038, 1612
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 66, 278
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 8 cut(s) 94, 242, 548, 786, 862, 1056, 1065, 1540
TspRI CASTG 4 cut(s) 25, 475, 1038, 1612
VneI GTGCAC 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 1177
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 4 cut(s) 1192, 1216, 1292, 1610
XmnI GAANNNNTTC 3 cut(s) 79, 539, 1344
XspI CTAG 6 cut(s) 168, 329, 398, 989, 1535, 1650
ZrmI AGTACT 1 cut(s) 1490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.