Rroxscaffold_152G00434590

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0000618
Physical Location & Seq
Reverse (-)
97292 .. 108197
10906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434590.1

Sequence Viewer

Length: 1767 bp
ATGCTTGGAGGTGCAGCTGGAGCTGGTGCCTATGCGCTGGGTATGATATCTGATGCCTTCAGCTCCTTGGCTTTCACTGCTCTGGCTATTTTCAACATCTTTACAAAGAAAACCAAGGTGATGCAGATTTTCCCAGAAAGTCTGAGTATATTATGGAGCTTGTGGGAAATCCGTGTTATGGTGCTTCTAAGTCTCATCTTACAAGGCATTCTCATCACGAATGGCAACCGGAGAAAGTATTCATGCAGCAACTGGCTCAGATTTCTGTGGTTTGCCTACTTGTCCGCAGATTGGGTTGCAACAGTCTCACTCAGTGTTCTTTCCAGCTATATTTCATCTGCGGACAATGGAACTAACAAATCGACAGACCCAAACAGCATCATCACAGCATTCTGGGCTCCGTTTCTCCTTCTCCACCTTGGCGGCCCAGATACTATTACCGCATACTCCTTGGAAGACAATGAGTTATGGTGGAGGCATTTGCTTGTACTAGTTGTCCAAGTATCTCTGGCTTTCTATGTATTTCTTAGGGCGTGGAGTGGCCATGCCCTGAACTTTCTAGCGATTCCAATGTTCGTTGCTGGAATTAACAAGTTTGCAGAAAGGACTTGGGTTTTGAGGTCTGCCAGCAGTAAGCACTTCAGAAAGTCAATGTTCCCTCGTCCTGATCCGGGGCCTGACTACGCCAGATACGTGGACGAGTATCGATCAAAGTCGGATGAAGGATTCAAGGTTAAGGTGGGGAAAGTAAAGGAAGCTCCCATCATAGGTGATTATTCCATTACAGCTCCCAGCAGCATTCCAAATGCAGCTGATTTGCAACACGCCCATCTTTTCTTCCAGATTTTCAAGAAGCTCTTTGCTGATCTCATTCTGAGTATCCATGATATTACGAACAGCCAATCCTTCTTCTGGAACATATCCTGTGATGAAGCTTTCAAGGTGATTGAGATTGAGCTTGGGTTTATGTATGATTTGTTTTATACCAAGGCAGCCCTTTATATAGTCAAGGCCGGTGCAATTGTTCGTTCTATCAGTTCTGTCTGCATCATTTATGTCTTTGTATCCTTCTTGACCATCAGCAAGCAAGAATACAAAGAAGCAAATGTGATCATCACTTATATGTTATTGGCTGGAGCTATCATCCTAGAGGTCTATGCTGTGTTTCAATTGCTGCGCTCGGATTGGACAATGCTATGGCTCACTAAGCACAAGAAAGCAGAGGATTTGTTGTATCCACTCATTTCATTCATTCCTTTAACTGGAGACAAAAGGTGGTCCAATGTCATCACCAAGTACAACTTTATAAGATTTTGCCTACAAGAGAAGCCGGCCAAATATTGCCGATGTCTCCCTAAGGTGCCATGGAGATACAGAACATACAAGAACGAAGAAAAGGTTCCTAGAGAATTGAAAGAATTGATATTTCAGCAGCTTATAAAGAAATCAAGGACTGAGGATTGCAAGAAAGTATGTGCTTATAGGGGTGACTGGGTACTTGAAAAGGAGGAGGATAAATCATGCCGCAAAAAACTTGGCTGGAGCATTGGAGAAGAATTTGATCAAAGCATTCTTCTTTGGCATATTGCAACACACCTCTGCTATTACTCTGATTTAAAAAGATACTCGAACTCTTTTAGTTATCCAACTATCTCTGATCCAGAAAATAACTCAAACTCTTTCTGCCATCCAAAATGTGACGATTTTTTTGGCAAGGGTCCAAAATGTGACTCTGATCTGGAAAAAATACTCAAATTGTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

588

Amino Acids

67.56

Weight (kDa)

8.9

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 90 - 437 5.3e-102 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1307, 1439
AccB1I GGYRCC 2 cut(s) 26, 1360
AciI CCGC 5 cut(s) 285, 341, 423, 441, 1525
AclWI GGATC 2 cut(s) 662, 1652
AcoI YGGCCR 2 cut(s) 541, 1332
AcsI RAATTY 1 cut(s) 1556
AcuI CTGAAG 2 cut(s) 43, 625
AdeI CACNNNGTG 1 cut(s) 314
AfaI GTAC 3 cut(s) 489, 1298, 1497
AfiI CCNNNNNNNGG 6 cut(s) 178, 291, 671, 767, 912, 1262
AgsI TTSAA 7 cut(s) 94, 730, 850, 940, 1169, 1414, 1502
AhlI ACTAGT 1 cut(s) 490
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
Alw26I GTCTC 4 cut(s) 197, 310, 1260, 1355
AlwI GGATC 2 cut(s) 662, 1652
AlwNI CAGNNNCTG 1 cut(s) 252
AoxI GGCC 5 cut(s) 424, 541, 674, 1011, 1332
ApeKI GCWGC 7 cut(s) 14, 246, 795, 809, 992, 1174, 1432
ApoI RAATTY 1 cut(s) 1556
ArsI GACNNNNNNTTYG 4 cut(s) 598, 630, 1691, 1723
Asp700I GAANNNNTTC 2 cut(s) 238, 1398
AspLEI GCGC 2 cut(s) 37, 1179
AspS9I GGNCC 4 cut(s) 425, 674, 1278, 1718
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 5 cut(s) 130, 782, 955, 1282, 1499
AvaII GGWCC 2 cut(s) 1278, 1718
AxyI CCTNAGG 1 cut(s) 1356
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 2 cut(s) 26, 1360
BanII GRGCYC 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 462
BbvI GCAGC 7 cut(s) 26, 258, 807, 821, 1004, 1161, 1444
BccI CCATC 4 cut(s) 770, 837, 1085, 1695
BciVI GTATCC 3 cut(s) 890, 1075, 1245
BclI TGATCA 2 cut(s) 1110, 1561
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 4 cut(s) 197, 310, 1260, 1355
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 4 cut(s) 491, 560, 1148, 1404
BfuI GTATCC 3 cut(s) 890, 1075, 1245
BisI GCNGC 9 cut(s) 15, 247, 424, 796, 810, 993, 1175, 1433, 1525
BlsI GCNGC 9 cut(s) 16, 248, 425, 797, 811, 994, 1176, 1434, 1526
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 2 cut(s) 1278, 1718
BmgT120I GGNCC 4 cut(s) 425, 674, 1278, 1718
BmiI GGNNCC 6 cut(s) 28, 399, 675, 1362, 1401, 1719
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 4 cut(s) 43, 111, 387, 1056
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 4 cut(s) 39, 1155, 1284, 1561
BpuMI CCSGG 1 cut(s) 672
Bsa29I ATCGAT 1 cut(s) 706
BsaAI YACGTR 1 cut(s) 694
BsaJI CCNNGG 7 cut(s) 66, 114, 418, 450, 671, 987, 1364
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 291, 671, 767, 912, 1262
Bse118I RCCGGY 2 cut(s) 1013, 1330
Bse1I ACTGG 3 cut(s) 257, 1267, 1496
Bse21I CCTNAGG 1 cut(s) 1356
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 7 cut(s) 66, 114, 418, 450, 671, 987, 1364
BseGI GGATG 3 cut(s) 724, 1143, 1687
BseLI CCNNNNNNNGG 6 cut(s) 178, 291, 671, 767, 912, 1262
BseMII CTCAG 5 cut(s) 134, 271, 325, 866, 1446
BseNI ACTGG 3 cut(s) 257, 1267, 1496
BseRI GAGGAG 1 cut(s) 1523
BseXI GCAGC 7 cut(s) 26, 258, 807, 821, 1004, 1161, 1444
BseYI CCCAGC 2 cut(s) 37, 791
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 5 cut(s) 426, 543, 676, 1013, 1334
BshNI GGYRCC 2 cut(s) 26, 1360
BshVI ATCGAT 1 cut(s) 706
BsiSI CCGG 4 cut(s) 229, 671, 1014, 1331
BslI CCNNNNNNNGG 6 cut(s) 178, 291, 671, 767, 912, 1262
BsmAI GTCTC 4 cut(s) 197, 310, 1260, 1355
BsmI GAATGC 4 cut(s) 207, 389, 798, 1569
BsnI GGCC 5 cut(s) 426, 543, 676, 1013, 1334
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
Bsp19I CCATGG 1 cut(s) 1364
BspACI CCGC 5 cut(s) 285, 341, 423, 441, 1525
BspANI GGCC 5 cut(s) 426, 543, 676, 1013, 1334
BspCNI CTCAG 5 cut(s) 135, 270, 324, 867, 1447
BspDI ATCGAT 1 cut(s) 706
BspLI GGNNCC 6 cut(s) 28, 399, 675, 1362, 1401, 1719
BspPI GGATC 2 cut(s) 662, 1652
BspT107I GGYRCC 2 cut(s) 26, 1360
BsrFI RCCGGY 2 cut(s) 1013, 1330
BsrI ACTGG 3 cut(s) 257, 1267, 1496
BssAI RCCGGY 2 cut(s) 1013, 1330
BssECI CCNNGG 7 cut(s) 66, 114, 418, 450, 671, 987, 1364
BssMI GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
BssT1I CCWWGG 6 cut(s) 66, 114, 418, 450, 987, 1364
Bst4CI ACNGT 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 694
BstC8I GCNNGC 3 cut(s) 628, 1085, 1332
BstDEI CTNAG 9 cut(s) 143, 188, 257, 311, 527, 875, 1206, 1356, 1455
BstDSI CCRYGG 1 cut(s) 1364
BstF5I GGATG 3 cut(s) 724, 1143, 1687
BstHHI GCGC 2 cut(s) 37, 1179
BstKTI GATC 7 cut(s) 670, 710, 868, 1113, 1564, 1660, 1738
BstMAI GTCTC 4 cut(s) 197, 310, 1260, 1355
BstMBI GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
BstMWI GCNNNNNNNGC 4 cut(s) 20, 77, 395, 1207
BstSCI CCNGG 1 cut(s) 670
BstV1I GCAGC 7 cut(s) 26, 258, 807, 821, 1004, 1161, 1444
BstV2I GAAGAC 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 694
Bsu15I ATCGAT 1 cut(s) 706
Bsu36I CCTNAGG 1 cut(s) 1356
BsuI GTATCC 3 cut(s) 890, 1075, 1245
BsuRI GGCC 5 cut(s) 426, 543, 676, 1013, 1334
BsuTUI ATCGAT 1 cut(s) 706
BtgI CCRYGG 1 cut(s) 1364
BtsCI GGATG 3 cut(s) 724, 1143, 1687
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 75, 319
Cac8I GCNNGC 3 cut(s) 628, 1085, 1332
CaiI CAGNNNCTG 1 cut(s) 252
CfoI GCGC 2 cut(s) 37, 1179
Cfr10I RCCGGY 2 cut(s) 1013, 1330
Cfr13I GGNCC 4 cut(s) 425, 674, 1278, 1718
ClaI ATCGAT 1 cut(s) 706
Csp6I GTAC 3 cut(s) 488, 1297, 1496
CviAII CATG 5 cut(s) 243, 545, 884, 1365, 1521
CviQI GTAC 3 cut(s) 488, 1297, 1496
DdeI CTNAG 9 cut(s) 143, 188, 257, 311, 527, 875, 1206, 1356, 1455
DpnI GATC 7 cut(s) 669, 709, 867, 1112, 1563, 1659, 1737
DpnII GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
DraI TTTAAA 1 cut(s) 1617
DraIII CACNNNGTG 1 cut(s) 314
EaeI YGGCCR 2 cut(s) 541, 1332
Eco130I CCWWGG 6 cut(s) 66, 114, 418, 450, 987, 1364
Eco24I GRGCYC 1 cut(s) 400
Eco32I GATATC 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 1278, 1718
Eco57I CTGAAG 2 cut(s) 43, 625
Eco81I CCTNAGG 1 cut(s) 1356
EcoO109I RGGNCCY 1 cut(s) 674
EcoRV GATATC 1 cut(s) 48
EcoT14I CCWWGG 6 cut(s) 66, 114, 418, 450, 987, 1364
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 6 cut(s) 66, 114, 418, 450, 987, 1364
FaeI CATG 5 cut(s) 246, 548, 887, 1368, 1524
FalI AAGNNNNNCTT 4 cut(s) 842, 874, 1286, 1318
FatI CATG 5 cut(s) 242, 544, 883, 1364, 1520
FbaI TGATCA 2 cut(s) 1110, 1561
Fnu4HI GCNGC 9 cut(s) 15, 247, 424, 796, 810, 993, 1175, 1433, 1525
FokI GGATG 3 cut(s) 731, 1130, 1674
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 9 cut(s) 15, 247, 424, 796, 810, 993, 1175, 1433, 1525
FspBI CTAG 4 cut(s) 491, 560, 1148, 1404
GlaI GCGC 2 cut(s) 36, 1178
GluI GCNGC 9 cut(s) 15, 247, 424, 796, 810, 993, 1175, 1433, 1525
GsaI CCCAGC 2 cut(s) 41, 795
GsuI CTGGAG 4 cut(s) 39, 1155, 1284, 1561
HaeIII GGCC 5 cut(s) 426, 543, 676, 1013, 1334
HapII CCGG 4 cut(s) 229, 671, 1014, 1331
HhaI GCGC 2 cut(s) 37, 1179
Hin1II CATG 5 cut(s) 246, 548, 887, 1368, 1524
Hin6I GCGC 2 cut(s) 35, 1177
HinP1I GCGC 2 cut(s) 35, 1177
HindIII AAGCTT 1 cut(s) 933
HinfI GANTC 3 cut(s) 565, 726, 1730
HpaII CCGG 4 cut(s) 229, 671, 1014, 1331
HphI GGTGA 5 cut(s) 130, 782, 955, 1282, 1499
Hpy166II GTNNAC 1 cut(s) 697
Hpy188III TCNNGA 8 cut(s) 217, 665, 841, 850, 913, 1072, 1661, 1739
Hpy8I GTNNAC 1 cut(s) 697
HpyAV CCTTC 5 cut(s) 67, 419, 716, 916, 1078
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 693
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 77, 395, 1207
HpyF3I CTNAG 9 cut(s) 143, 188, 257, 311, 527, 875, 1206, 1356, 1455
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 5 cut(s) 246, 548, 887, 1368, 1524
HspAI GCGC 2 cut(s) 35, 1177
KroI GCCGGC 1 cut(s) 1330
KroNI GCCGGC 1 cut(s) 1332
Ksp22I TGATCA 2 cut(s) 1110, 1561
Kzo9I GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
LmnI GCTCC 8 cut(s) 20, 68, 156, 403, 763, 793, 1136, 1542
Lsp1109I GCAGC 7 cut(s) 26, 258, 807, 821, 1004, 1161, 1444
LweI GCATC 4 cut(s) 43, 111, 387, 1056
MaeI CTAG 4 cut(s) 491, 560, 1148, 1404
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 3 cut(s) 1487, 1697, 1727
MalI GATC 7 cut(s) 669, 709, 867, 1112, 1563, 1659, 1737
MboI GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
MboII GAAGA 6 cut(s) 467, 829, 901, 1403, 1565, 1565
MfeI CAATTG 2 cut(s) 1020, 1169
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 7 cut(s) 585, 1020, 1169, 1409, 1418, 1556, 1754
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 1 cut(s) 1724
MmeI TCCRAC 2 cut(s) 696, 1670
MnlI CCTC 9 cut(s) 468, 612, 669, 1144, 1216, 1450, 1501, 1504, 1607
Mox20I TGGCCA 1 cut(s) 543
MroNI GCCGGC 1 cut(s) 1330
MroXI GAANNNNTTC 2 cut(s) 238, 1398
MscI TGGCCA 1 cut(s) 543
MseI TTAA 4 cut(s) 588, 735, 1259, 1616
MslI CAYNNNNRTG 1 cut(s) 1121
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 17, 812
MspI CCGG 4 cut(s) 229, 671, 1014, 1331
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 2 cut(s) 1020, 1169
Mva1269I GAATGC 4 cut(s) 207, 389, 798, 1569
MwoI GCNNNNNNNGC 4 cut(s) 20, 77, 395, 1207
NaeI GCCGGC 1 cut(s) 1332
NciI CCSGG 1 cut(s) 672
NcoI CCATGG 1 cut(s) 1364
NdeII GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
NgoMIV GCCGGC 1 cut(s) 1330
NlaIII CATG 5 cut(s) 246, 548, 887, 1368, 1524
NlaIV GGNNCC 6 cut(s) 28, 399, 675, 1362, 1401, 1719
NmuCI GTSAC 3 cut(s) 1487, 1697, 1727
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PctI GAATGC 4 cut(s) 207, 389, 798, 1569
PdiI GCCGGC 1 cut(s) 1332
PdmI GAANNNNTTC 2 cut(s) 238, 1398
PfeI GAWTC 2 cut(s) 565, 726
PkrI GCNGC 9 cut(s) 16, 248, 425, 797, 811, 994, 1176, 1434, 1526
PleI GAGTC 1 cut(s) 1724
PpsI GAGTC 1 cut(s) 1724
Ppu21I YACGTR 1 cut(s) 694
PsiI TTATAA 2 cut(s) 1307, 1439
PspFI CCCAGC 2 cut(s) 37, 791
PspN4I GGNNCC 6 cut(s) 28, 399, 675, 1362, 1401, 1719
PspPI GGNCC 4 cut(s) 425, 674, 1278, 1718
PstNI CAGNNNCTG 1 cut(s) 252
PvuII CAGCTG 2 cut(s) 17, 812
RsaI GTAC 3 cut(s) 489, 1298, 1497
RsaNI GTAC 3 cut(s) 488, 1297, 1496
RseI CAYNNNNRTG 1 cut(s) 1121
SaqAI TTAA 4 cut(s) 588, 735, 1259, 1616
SatI GCNGC 9 cut(s) 15, 247, 424, 796, 810, 993, 1175, 1433, 1525
Sau3AI GATC 7 cut(s) 667, 707, 865, 1110, 1561, 1657, 1735
Sau96I GGNCC 4 cut(s) 425, 674, 1278, 1718
SchI GAGTC 1 cut(s) 1724
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 4 cut(s) 43, 111, 387, 1056
SinI GGWCC 2 cut(s) 1278, 1718
SmiMI CAYNNNNRTG 1 cut(s) 1121
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 7 cut(s) 585, 1020, 1169, 1409, 1418, 1556, 1754
SsiI CCGC 5 cut(s) 285, 341, 423, 441, 1525
SspI AATATT 1 cut(s) 1340
SspMI CTAG 4 cut(s) 491, 560, 1148, 1404
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 6 cut(s) 66, 114, 418, 450, 987, 1364
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 696
TaqI TCGA 3 cut(s) 362, 706, 1628
TasI AATT 7 cut(s) 585, 1020, 1169, 1409, 1418, 1556, 1754
TatI WGTACW 2 cut(s) 487, 1296
TauI GCSGC 2 cut(s) 426, 1527
TfiI GAWTC 2 cut(s) 565, 726
Tru1I TTAA 4 cut(s) 588, 735, 1259, 1616
Tru9I TTAA 4 cut(s) 588, 735, 1259, 1616
TscAI CASTG 2 cut(s) 82, 319
TseFI GTSAC 3 cut(s) 1487, 1697, 1727
TseI GCWGC 7 cut(s) 14, 246, 795, 809, 992, 1174, 1432
Tsp45I GTSAC 3 cut(s) 1487, 1697, 1727
TspDTI ATGAA 6 cut(s) 231, 324, 735, 945, 1236, 1240
TspGWI ACGGA 2 cut(s) 161, 390
TspRI CASTG 2 cut(s) 82, 319
VpaK11BI GGWCC 2 cut(s) 1278, 1718
XapI RAATTY 1 cut(s) 1556
XmnI GAANNNNTTC 2 cut(s) 238, 1398
XspI CTAG 4 cut(s) 491, 560, 1148, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.