FvH4_3g37050

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000618
Physical Location & Seq
Reverse (-)
31773103 .. 31773696
594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37050.t1

Sequence Viewer

Length: 594 bp
ATGGGTGAACATGTACCGTTGGAGAAGACCATTCATATTTGGCACATTGCAACAGACTTATGCTTCTACAATGAACAAAAACACCCTGATCATCATGAGTACAAATCTCATGTGATGAGCAAGTTATTGTCGGACTATATGATGTATATTCTTGTCATGCAGCCCTTCTTGCTGCCAAAGGGGAGTGGGCATGTAAGGTTTAAGGACACCTGCGCACAGGCCAGAGAGTTTTTTAGACAGAGAAGACAAATCATAAGTAAAGGTGAAGCCGGCGGTCATCAAGTGTTGTCTGAAATGTGCAGAGAGTTGGATTCAGATGGTAGTGGGACAGATTCGAATATGGGAAGCAAGACAGTCTTGACACAAGGGTGTTGGCTTGCGGAAAAATTGCAGTCACAAGGATGGAACTATGAACAAAAATGGTATATAATAGGTGATGTGTGGATAGAGATGTTGTGTTACTCAGCAAGCCAATGTGGATGGAAGGAGCATGTTCGACAACTTAGGTGTGGCGGAGAGCTTCTTACTCTTGTCAGCCTTCTTATGGCAAATCTTGGATTCAGTGAACAATTTCAGCAATTCATACAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

198

Amino Acids

22.85

Weight (kDa)

6.19

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 136 - 186 1.8e-19 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 218
AciI CCGC 3 cut(s) 273, 380, 513
AfaI GTAC 2 cut(s) 15, 101
AfiI CCNNNNNNNGG 1 cut(s) 544
AflIII ACRYGT 1 cut(s) 10
AleI CACNNNNGTG 1 cut(s) 367
AluBI AGCT 1 cut(s) 520
AluI AGCT 1 cut(s) 520
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 160, 172
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 17, 275, 446
AsuII TTCGAA 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 32, 250
BbvI GCAGC 2 cut(s) 159, 172
BccI CCATC 3 cut(s) 311, 396, 474
BclI TGATCA 1 cut(s) 88
BfuAI ACCTGC 1 cut(s) 218
BisI GCNGC 2 cut(s) 161, 173
BlsI GCNGC 2 cut(s) 162, 174
BpiI GAAGAC 2 cut(s) 32, 250
Bpu14I TTCGAA 1 cut(s) 335
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse118I RCCGGY 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 45
BseGI GGATG 2 cut(s) 407, 485
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 159, 172
BsgI GTGCAG 1 cut(s) 319
BshFI GGCC 1 cut(s) 221
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 335
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 3 cut(s) 273, 380, 513
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 218
BspT104I TTCGAA 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssMI GATC 1 cut(s) 88
Bst4CI ACNGT 2 cut(s) 18, 355
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 3 cut(s) 271, 378, 469
BstDEI CTNAG 2 cut(s) 463, 503
BstF5I GGATG 2 cut(s) 407, 485
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNSI RCATGY 3 cut(s) 14, 194, 494
BstV1I GCAGC 2 cut(s) 159, 172
BstV2I GAAGAC 2 cut(s) 32, 250
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 2 cut(s) 407, 485
BtsIMutI CAGTG 1 cut(s) 568
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 3 cut(s) 271, 378, 469
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 269
Csp6I GTAC 2 cut(s) 14, 100
CviAII CATG 6 cut(s) 11, 95, 110, 157, 191, 491
CviJI RGCY 7 cut(s) 163, 221, 269, 376, 471, 520, 537
CviKI_1 RGCY 7 cut(s) 163, 221, 269, 376, 471, 520, 537
CviQI GTAC 2 cut(s) 14, 100
DdeI CTNAG 2 cut(s) 463, 503
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 528
FaeI CATG 6 cut(s) 14, 98, 113, 160, 194, 494
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 340
FatI CATG 6 cut(s) 10, 94, 109, 156, 190, 490
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 161, 173
FokI GGATG 2 cut(s) 414, 492
Fsp4HI GCNGC 2 cut(s) 161, 173
FspI TGCGCA 1 cut(s) 214
GlaI GCGC 1 cut(s) 214
GluI GCNGC 2 cut(s) 161, 173
HaeIII GGCC 1 cut(s) 221
HapII CCGG 1 cut(s) 270
HhaI GCGC 1 cut(s) 215
Hin1II CATG 6 cut(s) 14, 98, 113, 160, 194, 494
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 3 cut(s) 311, 332, 558
HpaII CCGG 1 cut(s) 270
HphI GGTGA 3 cut(s) 17, 275, 446
Hpy166II GTNNAC 2 cut(s) 8, 566
Hpy188I TCNGA 3 cut(s) 133, 292, 316
Hpy188III TCNNGA 2 cut(s) 95, 358
Hpy8I GTNNAC 2 cut(s) 8, 566
HpyAV CCTTC 3 cut(s) 175, 478, 548
HpyCH4III ACNGT 2 cut(s) 18, 355
HpyCH4V TGCA 4 cut(s) 50, 160, 300, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 2 cut(s) 463, 503
Hsp92II CATG 6 cut(s) 14, 98, 113, 160, 194, 494
HspAI GCGC 1 cut(s) 213
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 487
LpnPI CCDG 5 cut(s) 99, 203, 223, 235, 283
Lsp1109I GCAGC 2 cut(s) 159, 172
MaeIII GTNAC 2 cut(s) 393, 458
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 37, 255
MluCI AATT 3 cut(s) 386, 569, 578
MmeI TCCRAC 2 cut(s) 111, 288
MroNI GCCGGC 1 cut(s) 269
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 2 cut(s) 367, 400
MspI CCGG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 169
NaeI GCCGGC 1 cut(s) 271
NdeII GATC 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 6 cut(s) 14, 98, 113, 160, 194, 494
NmuCI GTSAC 1 cut(s) 393
NsbI TGCGCA 1 cut(s) 214
NspI RCATGY 3 cut(s) 14, 194, 494
NspV TTCGAA 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 367
PagI TCATGA 1 cut(s) 94
PaqCI CACCTGC 1 cut(s) 218
PciI ACATGT 1 cut(s) 10
PdiI GCCGGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 3 cut(s) 311, 332, 558
PkrI GCNGC 2 cut(s) 162, 174
PscI ACATGT 1 cut(s) 10
RsaI GTAC 2 cut(s) 15, 101
RsaNI GTAC 2 cut(s) 14, 100
RseI CAYNNNNRTG 2 cut(s) 367, 400
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 2 cut(s) 161, 173
Sau3AI GATC 1 cut(s) 88
SetI ASST 6 cut(s) 200, 212, 265, 436, 509, 522
SfuI TTCGAA 1 cut(s) 335
SmiMI CAYNNNNRTG 2 cut(s) 367, 400
Sse9I AATT 3 cut(s) 386, 569, 578
SsiI CCGC 3 cut(s) 273, 380, 513
TaaI ACNGT 2 cut(s) 18, 355
TaqI TCGA 2 cut(s) 335, 496
TasI AATT 3 cut(s) 386, 569, 578
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 3 cut(s) 311, 332, 558
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 1 cut(s) 393
TseI GCWGC 2 cut(s) 160, 172
Tsp45I GTSAC 1 cut(s) 393
TspDTI ATGAA 4 cut(s) 23, 87, 426, 571
TspRI CASTG 1 cut(s) 568
XceI RCATGY 3 cut(s) 14, 194, 494
XmnI GAANNNNTTC 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.