RLG00000035869

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000618
Physical Location & Seq
Reverse (-)
72903203 .. 72905329
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035869

Sequence Viewer

Length: 2127 bp
ATGGTGCAAATTATTCCACAAAGTGTGGACAAACTATGGAACGAATGGGACGTCCGGGTAATGGTGCTAAGTAGTCTCTGCTTACAAATAGTCCTAATCCTCTTGGGAAGCCGGCGAAAATTCAGTACTCAAAGCTGGGTCAGAATCCTCACATGGACGGCCTACTTATCGGCAGATTGGTTCGCTACCGTCGCGCTTGGAATGATCTCCACATTCCAAGGAGATGGTCAGCAAAACTCACCAACTCCAAACAGCGCTCAAGGAAGTTCCTCAAATCCAAACAGCCCGGCGATCATGGCTTTTTGGGCTCCGTTTCTTCTGGTGCACCTTGGTGGCCCGGACACCATCACTGCTTACTCGTTGGAGGACAACGAGCTGTGGCTAAGGCACCTCTTGGGTTTACTTGTTCAGGTTAGTGCCGCTTTATACATCTTTCTAAGATCATGGACATCAAGCTCCTTACCCCTCTCACTGCTAGCAATGCCAATGTTTGTTGTCGGAATCATCAAATATGGAGAGAGGACTTGGGCCCTTTGGTCTGCTAGCAGCGAGAGACTTAGGGAATCTCTGCCCCCTATTACCGCAGCTCTGACTCCCTTTAAAAATGAGAATCATGAGAGTAGTGGTCGTGTTAGACTAAACTATGCTGCTGCTATTCGTAAAGCCACACATATCCAAGAAGGTCATGCATTATTCAAGTTGTTCAGGCATTTTTACACAGATCAGATCATTGAACAAAAGGATGTTCAACTTTCCGATACTTTGATTCAGGACCATTCATTTGGAGATGTCTTCAAGCTAATTGAGATTGAGATTGGTTTTATGTATGATGTGCTGTACACCAAGGCAATCGTAGTGCATTCTCTCACGGGGATCATGTTCCGAGCCATCTCGCTGGTTTGCTACATTTCCACATTTACGGTCTTTTGTTTCATCATTGATAAGAAGATTTATTCGACCATTGATGTAGATTTGACTTACTTCTTGTGTGCTGGAGCAATTGCACTTGAGATCTATGCCATCTTTGTACAAGTTTCTACAGATTGGACATTGTACTGGTTAAGCAAGCAAGAGCGACCGCTGGCGTATACTATCTACCAAGCAATCTCTTCATTTCGATCAAATCTGAGCCTTGAAAAGAGGTGGTCTGAATCCATGGGCCAATTCAATGTCATAGAGTTTTGTTTCAAATTGAAACCGCCAAAGTGTACTGGAATTCCTAGGTTCCTGGCTATCTACGAGATGTATTCTAAACATCAGCACACAGTTTTGGAGGGCGTGCCAATCAAAATGAAACAACTTATATTTGAGCACCTTCAAGATGCTAAACTAGTTGTTGACAATGCAGAGCAGAATTCCAAATTTCAAGTACGTAGTCAAGTCTTGACTTGGAGAGGTGAGCATGCGGTACAAAAGAGTGGATGTACTGACAAATATATCAAACATTGTGCCGCGAATGAAGTACCGTTTGAAACCAGCATTGCTTTGTGGCATGTAGCAACAGATATATGCTTCTATAATGATGTTGACAAAAATCAAAAGGCCTCTGATCCTAATTCCAAATCCGAGGTGAGCAAGTTGCTGTCAGATTATATGATGTATATTCTTGCCATCTGCCCTTTCATGTTGCCCCAAGGGATTGGACAGATTTTATTTCGACAAATATGTGAGTCTGCCACAACATTTTTTGACGAGAGAAGGGAAGCCATAAGTAAGACAGAGCGATTGGTTGGAAAAAGGGCCAGTCAGGTGTTGTACGAGATGAAGTCAGAGTTGAATCCATGGCTAGATGAGTTTGAGTCCGGAGATGGAGGAAAACTAAACAATGTAGTGTTGCTTGAAGGGTGTGTGCTTGCACATAAGTTGCAGGCCCTGGAAACGGAGGAGAGCTGGACTAATGAAGATAAATGGGGAATGATAAGTCGAGTGTGGATAGAAATGTTGTGTTATGCAGCAAGCCAATGTGGATGGACGGAGCACGGTCAACAACTTAGGCGTGGCGGAGAGCTTCTTACTCATGTCTCTCTTCTCATGGCAAATCTTGGTATCGGTGAACAGAGTAAGATATTGAGTGAGCAGGACATGGACCGGTTACAGGATTTAAAAAACCTGTGTGAAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

709

Amino Acids

80.72

Weight (kDa)

6.06

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 393 1.9e-94 Domain of unknown function (DUF4220)
DUF594 PF04578 631 - 683 3.1e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 387
AccI GTMKAC 1 cut(s) 1088
AccII CGCG 2 cut(s) 194, 1454
AccIII TCCGGA 1 cut(s) 1802
AciI CCGC 7 cut(s) 420, 582, 1079, 1199, 1406, 1452, 2001
AclWI GGATC 2 cut(s) 881, 1544
AcsI RAATTY 4 cut(s) 119, 1215, 1354, 1361
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 23
AfeI AGCGCT 1 cut(s) 256
AfiI CCNNNNNNNGG 3 cut(s) 61, 1879, 2095
AgeI ACCGGT 1 cut(s) 2088
AhlI ACTAGT 1 cut(s) 1330
AjnI CCWGG 2 cut(s) 1227, 1872
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 7 cut(s) 135, 376, 456, 587, 799, 1890, 2008
AluI AGCT 7 cut(s) 135, 376, 456, 587, 799, 1890, 2008
Alw21I GWGCWC 3 cut(s) 327, 1314, 1980
Alw26I GTCTC 3 cut(s) 80, 547, 2026
Alw44I GTGCAC 1 cut(s) 323
AlwI GGATC 2 cut(s) 881, 1544
AlwNI CAGNNNCTG 1 cut(s) 1873
Aor13HI TCCGGA 1 cut(s) 1802
Aor51HI AGCGCT 1 cut(s) 256
AoxI GGCC 7 cut(s) 159, 334, 528, 1159, 1542, 1740, 1869
ApaI GGGCCC 1 cut(s) 532
ApaLI GTGCAC 1 cut(s) 323
ApeKI GCWGC 5 cut(s) 546, 584, 647, 650, 1952
ApoI RAATTY 4 cut(s) 119, 1215, 1354, 1361
ArsI GACNNNNNNTTYG 4 cut(s) 36, 68, 764, 796
AsiGI ACCGGT 1 cut(s) 2088
AspA2I CCTAGG 1 cut(s) 1220
AspLEI GCGC 2 cut(s) 196, 257
AspS9I GGNCC 8 cut(s) 335, 528, 529, 772, 1159, 1740, 1870, 2086
AsuC2I CCSGG 3 cut(s) 56, 287, 338
AsuHPI GGTGA 4 cut(s) 231, 1409, 1582, 2063
AsuNHI GCTAGC 2 cut(s) 475, 542
AvaII GGWCC 2 cut(s) 772, 2086
AvrII CCTAGG 1 cut(s) 1220
BaeGI GKGCMC 2 cut(s) 327, 532
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 2 cut(s) 310, 532
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 3 cut(s) 327, 1314, 1980
BbvI GCAGC 5 cut(s) 558, 596, 634, 637, 1964
BccI CCATC 7 cut(s) 218, 353, 896, 1028, 1619, 1802, 1962
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 2 cut(s) 1229, 1874
BcnI CCSGG 3 cut(s) 56, 287, 338
BcoDI GTCTC 3 cut(s) 80, 547, 2026
BcuI ACTAGT 1 cut(s) 1330
BfaI CTAG 5 cut(s) 476, 543, 1221, 1331, 1787
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 258
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 7 cut(s) 420, 547, 585, 648, 651, 1452, 1953
BlnI CCTAGG 1 cut(s) 1220
BlsI GCNGC 7 cut(s) 421, 548, 586, 649, 652, 1453, 1954
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 5 cut(s) 56, 287, 338, 1229, 1874
Bme18I GGWCC 2 cut(s) 772, 2086
BmgT120I GGNCC 8 cut(s) 335, 528, 529, 772, 1159, 1740, 1870, 2086
BmiI GGNNCC 4 cut(s) 309, 389, 530, 1226
BmrFI CCNGG 5 cut(s) 56, 287, 338, 1229, 1874
BmsI GCATC 1 cut(s) 1312
BmtI GCTAGC 2 cut(s) 479, 546
BpiI GAAGAC 1 cut(s) 784
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 243, 1028
BpuMI CCSGG 3 cut(s) 56, 287, 338
BsaAI YACGTR 1 cut(s) 1373
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 9 cut(s) 217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BsaWI WCCGGW 2 cut(s) 1802, 2088
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 1879, 2095
Bse118I RCCGGY 2 cut(s) 111, 2088
Bse1I ACTGG 3 cut(s) 1061, 1216, 1743
Bse3DI GCAATG 2 cut(s) 486, 1479
BseAI TCCGGA 1 cut(s) 1802
BseBI CCWGG 2 cut(s) 1229, 1874
BseDI CCNNGG 9 cut(s) 217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BseGI GGATG 3 cut(s) 748, 1427, 1973
BseLI CCNNNNNNNGG 3 cut(s) 61, 1879, 2095
BseMI GCAATG 2 cut(s) 486, 1479
BseMII CTCAG 1 cut(s) 1118
BseNI ACTGG 3 cut(s) 1061, 1216, 1743
BseRI GAGGAG 1 cut(s) 1898
BseSI GKGCMC 2 cut(s) 327, 532
BseXI GCAGC 5 cut(s) 558, 596, 634, 637, 1964
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 2 cut(s) 194, 1454
Bsh1285I CGRYCG 1 cut(s) 1079
BshFI GGCC 7 cut(s) 161, 336, 530, 1161, 1544, 1742, 1871
BshNI GGYRCC 1 cut(s) 387
BshTI ACCGGT 1 cut(s) 2088
BsiEI CGRYCG 1 cut(s) 1079
BsiHKAI GWGCWC 3 cut(s) 327, 1314, 1980
BsiSI CCGG 6 cut(s) 55, 112, 287, 338, 1803, 2089
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 61, 1879, 2095
BsmAI GTCTC 3 cut(s) 80, 547, 2026
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 7 cut(s) 161, 336, 530, 1161, 1544, 1742, 1871
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 5 cut(s) 310, 327, 532, 1314, 1980
Bsp13I TCCGGA 1 cut(s) 1802
Bsp1407I TGTACA 2 cut(s) 837, 1027
Bsp143I GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
Bsp19I CCATGG 2 cut(s) 1155, 1781
BspACI CCGC 7 cut(s) 420, 582, 1079, 1199, 1406, 1452, 2001
BspANI GGCC 7 cut(s) 161, 336, 530, 1161, 1544, 1742, 1871
BspCNI CTCAG 1 cut(s) 1119
BspEI TCCGGA 1 cut(s) 1802
BspFNI CGCG 2 cut(s) 194, 1454
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 309, 389, 530, 1226
BspOI GCTAGC 2 cut(s) 479, 546
BspPI GGATC 2 cut(s) 881, 1544
BspT107I GGYRCC 1 cut(s) 387
BsrDI GCAATG 2 cut(s) 486, 1479
BsrFI RCCGGY 2 cut(s) 111, 2088
BsrGI TGTACA 2 cut(s) 837, 1027
BsrI ACTGG 3 cut(s) 1061, 1216, 1743
BssAI RCCGGY 2 cut(s) 111, 2088
BssECI CCNNGG 9 cut(s) 217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BssMI GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
BssNAI GTATAC 1 cut(s) 1089
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 7 cut(s) 217, 328, 843, 1155, 1220, 1633, 1781
Bst1107I GTATAC 1 cut(s) 1089
Bst2UI CCWGG 2 cut(s) 1229, 1874
Bst4CI ACNGT 5 cut(s) 190, 922, 1267, 1467, 1982
Bst6I CTCTTC 2 cut(s) 1114, 2031
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 837, 1027
BstBAI YACGTR 1 cut(s) 1373
BstDEI CTNAG 6 cut(s) 68, 383, 437, 557, 1127, 1991
BstDSI CCRYGG 2 cut(s) 1155, 1781
BstF5I GGATG 3 cut(s) 748, 1427, 1973
BstFNI CGCG 2 cut(s) 194, 1454
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 196, 257
BstKTI GATC 9 cut(s) 207, 294, 443, 724, 729, 876, 1014, 1121, 1552
BstMAI GTCTC 3 cut(s) 80, 547, 2026
BstMBI GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
BstMCI CGRYCG 1 cut(s) 1079
BstMWI GCNNNNNNNGC 4 cut(s) 191, 296, 305, 481
BstNI CCWGG 2 cut(s) 1229, 1874
BstNSI RCATGY 2 cut(s) 1406, 1496
BstSCI CCNGG 5 cut(s) 54, 285, 336, 1227, 1872
BstSFI CTRYAG 1 cut(s) 1038
BstSLI GKGCMC 2 cut(s) 327, 532
BstSNI TACGTA 1 cut(s) 1373
BstUI CGCG 2 cut(s) 194, 1454
BstV1I GCAGC 5 cut(s) 558, 596, 634, 637, 1964
BstV2I GAAGAC 1 cut(s) 784
BstX2I RGATCY 1 cut(s) 1011
BstXI CCANNNNNNTGG 4 cut(s) 224, 782, 895, 1640
BstYI RGATCY 1 cut(s) 1011
BstZ17I GTATAC 1 cut(s) 1089
BsuRI GGCC 7 cut(s) 161, 336, 530, 1161, 1544, 1742, 1871
BtgI CCRYGG 2 cut(s) 1155, 1781
BtsCI GGATG 3 cut(s) 748, 1427, 1973
BtsI GCAGTG 2 cut(s) 348, 470
BtsIMutI CAGTG 2 cut(s) 348, 470
CaiI CAGNNNCTG 1 cut(s) 1873
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 196, 257
Cfr10I RCCGGY 2 cut(s) 111, 2088
Cfr13I GGNCC 8 cut(s) 335, 528, 529, 772, 1159, 1740, 1870, 2086
CspAI ACCGGT 1 cut(s) 2088
DdeI CTNAG 6 cut(s) 68, 383, 437, 557, 1127, 1991
DpnI GATC 9 cut(s) 206, 293, 442, 723, 728, 875, 1013, 1120, 1551
DpnII GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
DraI TTTAAA 2 cut(s) 601, 2103
DraIII CACNNNGTG 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 1114, 2031
EarI CTCTTC 2 cut(s) 1114, 2031
EciI GGCGGA 1 cut(s) 2016
Eco105I TACGTA 1 cut(s) 1373
Eco130I CCWWGG 7 cut(s) 217, 328, 843, 1155, 1220, 1633, 1781
Eco147I AGGCCT 1 cut(s) 1544
Eco24I GRGCYC 2 cut(s) 310, 532
Eco47I GGWCC 2 cut(s) 772, 2086
Eco47III AGCGCT 1 cut(s) 256
EcoO109I RGGNCCY 2 cut(s) 529, 1870
EcoRI GAATTC 2 cut(s) 1215, 1354
EcoRII CCWGG 2 cut(s) 1227, 1872
EcoT14I CCWWGG 7 cut(s) 217, 328, 843, 1155, 1220, 1633, 1781
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 2 cut(s) 310, 532
ErhI CCWWGG 7 cut(s) 217, 328, 843, 1155, 1220, 1633, 1781
FaqI GGGAC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 1088
Fnu4HI GCNGC 7 cut(s) 420, 547, 585, 648, 651, 1452, 1953
FokI GGATG 3 cut(s) 755, 1434, 1980
FriOI GRGCYC 2 cut(s) 310, 532
Fsp4HI GCNGC 7 cut(s) 420, 547, 585, 648, 651, 1452, 1953
FspBI CTAG 5 cut(s) 476, 543, 1221, 1331, 1787
GlaI GCGC 2 cut(s) 195, 256
GluI GCNGC 7 cut(s) 420, 547, 585, 648, 651, 1452, 1953
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 7 cut(s) 161, 336, 530, 1161, 1544, 1742, 1871
HapII CCGG 6 cut(s) 55, 112, 287, 338, 1803, 2089
HhaI GCGC 2 cut(s) 196, 257
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 2 cut(s) 194, 255
HinP1I GCGC 2 cut(s) 194, 255
HincII GTYRAC 3 cut(s) 1339, 1528, 1985
HindII GTYRAC 3 cut(s) 1339, 1528, 1985
HpaII CCGG 6 cut(s) 55, 112, 287, 338, 1803, 2089
HphI GGTGA 4 cut(s) 231, 1409, 1582, 2063
Hpy188III TCNNGA 5 cut(s) 614, 770, 1319, 1384, 1803
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 4 cut(s) 674, 1325, 1692, 1835
HpyCH4III ACNGT 5 cut(s) 190, 922, 1267, 1467, 1982
HpyCH4IV ACGT 2 cut(s) 51, 1372
HpyCH4V TGCA 9 cut(s) 7, 325, 689, 859, 1004, 1346, 1856, 1867, 1952
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 296, 305, 481
HpyF3I CTNAG 6 cut(s) 68, 383, 437, 557, 1127, 1991
HpySE526I ACGT 2 cut(s) 51, 1372
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 2 cut(s) 194, 255
Kpn2I TCCGGA 1 cut(s) 1802
KroI GCCGGC 1 cut(s) 111
KroNI GCCGGC 1 cut(s) 113
Kzo9I GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
LmnI GCTCC 4 cut(s) 313, 461, 995, 1975
Lsp1109I GCAGC 5 cut(s) 558, 596, 634, 637, 1964
LweI GCATC 1 cut(s) 1312
MaeI CTAG 5 cut(s) 476, 543, 1221, 1331, 1787
MaeII ACGT 2 cut(s) 51, 1372
MaeIII GTNAC 1 cut(s) 2091
MalI GATC 9 cut(s) 206, 293, 442, 723, 728, 875, 1013, 1120, 1551
MboI GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
MboII GAAGA 6 cut(s) 308, 784, 958, 1101, 1913, 2018
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 1 cut(s) 1011
MhlI GDGCHC 5 cut(s) 310, 327, 532, 1314, 1980
MlyI GAGTC 3 cut(s) 586, 1679, 1808
MmeI TCCRAC 3 cut(s) 342, 478, 1711
Mph1103I ATGCAT 1 cut(s) 691
MroI TCCGGA 1 cut(s) 1802
MroNI GCCGGC 1 cut(s) 111
MseI TTAA 3 cut(s) 600, 1061, 2102
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 1081
MspI CCGG 6 cut(s) 55, 112, 287, 338, 1803, 2089
MspR9I CCNGG 5 cut(s) 56, 287, 338, 1229, 1874
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 2 cut(s) 1229, 1874
MvnI CGCG 2 cut(s) 194, 1454
MwoI GCNNNNNNNGC 4 cut(s) 191, 296, 305, 481
NaeI GCCGGC 1 cut(s) 113
NciI CCSGG 3 cut(s) 56, 287, 338
NcoI CCATGG 2 cut(s) 1155, 1781
NdeII GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
NgoMIV GCCGGC 1 cut(s) 111
NheI GCTAGC 2 cut(s) 475, 542
NlaIV GGNNCC 4 cut(s) 309, 389, 530, 1226
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 2 cut(s) 1406, 1496
OliI CACNNNNGTG 1 cut(s) 330
PaeI GCATGC 1 cut(s) 1406
PagI TCATGA 1 cut(s) 613
PceI AGGCCT 1 cut(s) 1544
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 859
PdiI GCCGGC 1 cut(s) 113
PfeI GAWTC 7 cut(s) 144, 501, 563, 610, 766, 1151, 1777
PinAI ACCGGT 1 cut(s) 2088
PkrI GCNGC 7 cut(s) 421, 548, 586, 649, 652, 1453, 1954
PleI GAGTC 3 cut(s) 586, 1678, 1807
PpsI GAGTC 3 cut(s) 586, 1678, 1807
Ppu21I YACGTR 1 cut(s) 1373
Psp6I CCWGG 2 cut(s) 1227, 1872
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 2 cut(s) 1227, 1872
PspN4I GGNNCC 4 cut(s) 309, 389, 530, 1226
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 8 cut(s) 335, 528, 529, 772, 1159, 1740, 1870, 2086
PstNI CAGNNNCTG 1 cut(s) 1873
PsuI RGATCY 1 cut(s) 1011
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 3 cut(s) 600, 1061, 2102
SatI GCNGC 7 cut(s) 420, 547, 585, 648, 651, 1452, 1953
Sau3AI GATC 9 cut(s) 204, 291, 440, 721, 726, 873, 1011, 1118, 1549
Sau96I GGNCC 8 cut(s) 335, 528, 529, 772, 1159, 1740, 1870, 2086
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 3 cut(s) 586, 1679, 1808
ScrFI CCNGG 5 cut(s) 56, 287, 338, 1229, 1874
SduI GDGCHC 5 cut(s) 310, 327, 532, 1314, 1980
SfaNI GCATC 1 cut(s) 1312
SfcI CTRYAG 1 cut(s) 1038
SinI GGWCC 2 cut(s) 772, 2086
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 258, 1007
SmoI CTYRAG 2 cut(s) 258, 1007
SnaBI TACGTA 1 cut(s) 1373
SpeI ACTAGT 1 cut(s) 1330
SphI GCATGC 1 cut(s) 1406
SseBI AGGCCT 1 cut(s) 1544
SsiI CCGC 7 cut(s) 420, 582, 1079, 1199, 1406, 1452, 2001
SspMI CTAG 5 cut(s) 476, 543, 1221, 1331, 1787
StuI AGGCCT 1 cut(s) 1544
StyD4I CCNGG 5 cut(s) 54, 285, 336, 1227, 1872
StyI CCWWGG 7 cut(s) 217, 328, 843, 1155, 1220, 1633, 1781
TaaI ACNGT 5 cut(s) 190, 922, 1267, 1467, 1982
TaiI ACGT 2 cut(s) 54, 1375
TaqI TCGA 4 cut(s) 956, 1117, 1657, 1924
TatI WGTACW 6 cut(s) 125, 837, 1027, 1053, 1208, 1424
TauI GCSGC 2 cut(s) 422, 1454
TfiI GAWTC 7 cut(s) 144, 501, 563, 610, 766, 1151, 1777
Tru1I TTAA 3 cut(s) 600, 1061, 2102
Tru9I TTAA 3 cut(s) 600, 1061, 2102
TscAI CASTG 2 cut(s) 355, 477
TseI GCWGC 5 cut(s) 546, 584, 647, 650, 1952
TspDTI ATGAA 8 cut(s) 768, 922, 1101, 1307, 1473, 1612, 1778, 1914
TspGWI ACGGA 3 cut(s) 300, 1895, 1988
TspRI CASTG 2 cut(s) 355, 477
VneI GTGCAC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 772, 2086
XapI RAATTY 4 cut(s) 119, 1215, 1354, 1361
XceI RCATGY 2 cut(s) 1406, 1496
XmaJI CCTAGG 1 cut(s) 1220
XmiI GTMKAC 1 cut(s) 1088
XspI CTAG 5 cut(s) 476, 543, 1221, 1331, 1787
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.