Rh2CG432200

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000618
Physical Location & Seq
Reverse (-)
58826801 .. 58833050
6250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG432200.1

Sequence Viewer

Length: 513 bp
ATGTGGAATCTTAGGGACTTAGAGATTAGATCCTGCAGGAGCTTGACATCTACTTCTGGATTAACACATTTGAAGATCCTACAGGAGTTGAAGTTAACTAATATGCCTAAGAATACACGGGCCATGTGGAAAATGACTTTGCAGAAGCTGCACTACGGTCTGCTGGCAAAGCTATTCACTGTCATGCAATCAAAATTGATAATTGGCAACAGGAGAAAGCATTCAACCAGAAACTGGCATAGGTTTATTCTCTGGCTTGCCTACTTGTCCGCAAAGTGGATTGCAACACTCTCATTCAACCTACTTTCCAGCAATTCGATAGACCATGATGCGAATTCCAAAGACCTAGACTACATCATCACAGCATTTTGGGCTCCATTTCTTCTTCTTCACCTTGGTGGCCCAGACACCATTACCGCGTCATCTTTGGAAGACAATGAGATGTGGTGGAGGCACTTCCTTGTGCTACTAGTCCAGGTTGCAGTCGCTTTCTACATCTTCCTCATAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

170

Amino Acids

19.85

Weight (kDa)

9.88

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 85 - 168 2e-27 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 234
AccII CGCG 1 cut(s) 419
AciI CCGC 2 cut(s) 270, 417
AclWI GGATC 2 cut(s) 24, 70
AcsI RAATTY 1 cut(s) 334
AfiI CCNNNNNNNGG 2 cut(s) 234, 276
AgsI TTSAA 4 cut(s) 73, 91, 225, 298
AhlI ACTAGT 1 cut(s) 469
AjnI CCWGG 1 cut(s) 474
AleI CACNNNNGTG 1 cut(s) 396
AluBI AGCT 3 cut(s) 42, 148, 172
AluI AGCT 3 cut(s) 42, 148, 172
AlwI GGATC 2 cut(s) 24, 70
AlwNI CAGNNNCTG 2 cut(s) 148, 234
AoxI GGCC 2 cut(s) 120, 400
ApeKI GCWGC 1 cut(s) 148
ApoI RAATTY 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 120, 401
AsuHPI GGTGA 1 cut(s) 383
BanII GRGCYC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 137, 169
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 135
BciT130I CCWGG 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 3 cut(s) 347, 470, 511
BfmI CTRYAG 2 cut(s) 34, 80
BisI GCNGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 476
BmgT120I GGNCC 2 cut(s) 120, 401
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 276
Bse1I ACTGG 1 cut(s) 239
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 234, 276
BseNI ACTGG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 2 cut(s) 122, 402
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 2 cut(s) 234, 276
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 2 cut(s) 122, 402
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 2 cut(s) 29, 75
BspACI CCGC 2 cut(s) 270, 417
BspANI GGCC 2 cut(s) 122, 402
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 1 cut(s) 375
BspMAI CTGCAG 1 cut(s) 38
BspPI GGATC 2 cut(s) 24, 70
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 2 cut(s) 29, 75
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 158, 181
BstAPI GCANNNNNTGC 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 165, 258
BstDEI CTNAG 3 cut(s) 11, 19, 108
BstFNI CGCG 1 cut(s) 419
BstKTI GATC 2 cut(s) 32, 78
BstMBI GATC 2 cut(s) 29, 75
BstMWI GCNNNNNNNGC 3 cut(s) 148, 169, 371
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 474
BstSFI CTRYAG 2 cut(s) 34, 80
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 29, 75
BstYI RGATCY 2 cut(s) 29, 75
BsuRI GGCC 2 cut(s) 122, 402
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 165, 258
CaiI CAGNNNCTG 2 cut(s) 148, 234
Cfr13I GGNCC 2 cut(s) 120, 401
CseI GACGC 1 cut(s) 408
CviAII CATG 3 cut(s) 124, 184, 326
CviJI RGCY 8 cut(s) 42, 122, 148, 172, 256, 374, 402, 509
CviKI_1 RGCY 8 cut(s) 42, 122, 148, 172, 256, 374, 402, 509
DdeI CTNAG 3 cut(s) 11, 19, 108
DpnI GATC 2 cut(s) 31, 77
DpnII GATC 2 cut(s) 29, 75
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 3 cut(s) 127, 187, 329
FaiI YATR 6 cut(s) 104, 125, 185, 240, 327, 506
FaqI GGGAC 1 cut(s) 29
FatI CATG 3 cut(s) 123, 183, 325
Fnu4HI GCNGC 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 1 cut(s) 149
FspBI CTAG 3 cut(s) 347, 470, 511
GluI GCNGC 1 cut(s) 149
HaeIII GGCC 2 cut(s) 122, 402
HgaI GACGC 1 cut(s) 408
Hin1II CATG 3 cut(s) 127, 187, 329
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 1 cut(s) 7
HpaI GTTAAC 1 cut(s) 96
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 96
Hpy188III TCNNGA 1 cut(s) 57
Hpy8I GTNNAC 1 cut(s) 96
HpyCH4III ACNGT 2 cut(s) 158, 181
HpyCH4V TGCA 6 cut(s) 36, 142, 151, 187, 284, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 169, 371
HpyF3I CTNAG 3 cut(s) 11, 19, 108
Hsp92II CATG 3 cut(s) 127, 187, 329
KspAI GTTAAC 1 cut(s) 96
Kzo9I GATC 2 cut(s) 29, 75
LmnI GCTCC 2 cut(s) 39, 379
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 319
MaeI CTAG 3 cut(s) 347, 470, 511
MalI GATC 2 cut(s) 31, 77
MboI GATC 2 cut(s) 29, 75
MboII GAAGA 6 cut(s) 85, 374, 377, 380, 443, 490
MflI RGATCY 2 cut(s) 29, 75
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 4 cut(s) 194, 201, 313, 334
MnlI CCTC 2 cut(s) 444, 512
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 2 cut(s) 62, 95
MslI CAYNNNNRTG 2 cut(s) 182, 396
MspR9I CCNGG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 3 cut(s) 148, 169, 371
NdeII GATC 2 cut(s) 29, 75
NlaIII CATG 3 cut(s) 127, 187, 329
NlaIV GGNNCC 1 cut(s) 375
OliI CACNNNNGTG 1 cut(s) 396
PctI GAATGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 2 cut(s) 120, 401
PstI CTGCAG 1 cut(s) 38
PstNI CAGNNNCTG 2 cut(s) 148, 234
PsuI RGATCY 2 cut(s) 29, 75
RseI CAYNNNNRTG 2 cut(s) 182, 396
SaqAI TTAA 2 cut(s) 62, 95
SatI GCNGC 1 cut(s) 149
Sau3AI GATC 2 cut(s) 29, 75
Sau96I GGNCC 2 cut(s) 120, 401
SbfI CCTGCAGG 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 476
SdaI CCTGCAGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 376
SetI ASST 8 cut(s) 44, 150, 174, 245, 303, 348, 396, 480
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 2 cut(s) 34, 80
SmiMI CAYNNNNRTG 2 cut(s) 182, 396
SpeI ACTAGT 1 cut(s) 469
Sse8387I CCTGCAGG 1 cut(s) 38
Sse9I AATT 4 cut(s) 194, 201, 313, 334
SsiI CCGC 2 cut(s) 270, 417
SspMI CTAG 3 cut(s) 347, 470, 511
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 2 cut(s) 158, 181
TaqI TCGA 1 cut(s) 317
TasI AATT 4 cut(s) 194, 201, 313, 334
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 62, 95
Tru9I TTAA 2 cut(s) 62, 95
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 148
TspRI CASTG 1 cut(s) 184
Van91I CCANNNNNTGG 1 cut(s) 234
XapI RAATTY 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 3 cut(s) 347, 470, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.