Rh2AG446400

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000618
Physical Location & Seq
Forward (+)
65936192 .. 65942929
6738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG446400.1

Sequence Viewer

Length: 960 bp
ATGCAGATTTTCCCAGAAAACCTGAGCATATTATGGAACTCGTGGGAAATCCGTGTTATGGTGCTATCAAGCCTCATGTTACAGGCCATTCTCATGCTGTTCGGCAACTGGAGAAAGTTCTGCACCAGCAAATGGCTCAAGTTACTGTGGCTTGCCTACTTGTCCGCAGATTGGGTTGCAACAGTCTCGCTCAGCATACTCTCCAGTTCCAGCAATTCTAAGGAGAATGGAATCAAATCTGTAGACCCTAACCAGGTTCTCTCGGCATTCTGGGCTCCTTTTCTGCTGCTTCACCTTGGTGGCCAAGACACCATTACCGCATACTCATTGGAAGACAATGAGTTGTGGTGGAGGCACCTATTAGTACTAGTGGTTCAGGTGACTATAGCTTTTTATGTCATTTTAAGAGCTTGGACTGATCATGCGCTTAACTATCTAGCTATTCCAATCTTCGCCTCCGGGATTATCAAATTTGCGGAACGAACTTGGGTTTTCAGGTCTGCAAGCAGTAAGCACTTCAGAGAGTCTATGTTTGCTGAACCTGACCCTGGGCCGAACTACTCCAAATTCATGGATGAATACCTATCCAAAAAGAATCAAGGATTGGAGGTTAACTTAGGGAGAGTGATTGAAGTTCCCACCGGAGCTGATTATTCCTTCACCCCTACAGCTCCCAACATCATTTCAAATGCAGCCAATTTGCAACATGCCCATCATTTCTTCGAGGCTCAGTTTTTCAAGCGACTATTTGCTGATCTCGTACTCGGCATCTCTGAAATTTTTTACAGCCAATCCTTCTTCAAGAACAGATCCTCTGATGAAGCTTTCAGAGTTATTGAGATTGAGCTCGGTTTTATATTGGGCAATGCTATGGCTGAGCAAGTTCTGGGAGGTGGGTTTCTTGTATCCAGTTGTTTCACCTCTCTTGTCTTCCTCTGCTCAGAGAAAAAGATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

319

Amino Acids

36.27

Weight (kDa)

6.17

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 50 - 286 7.5e-67 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 132
AccI GTMKAC 1 cut(s) 243
AciI CCGC 3 cut(s) 165, 318, 476
AclWI GGATC 1 cut(s) 804
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 3 cut(s) 470, 566, 777
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 2 cut(s) 366, 762
AfiI CCNNNNNNNGG 5 cut(s) 58, 132, 171, 253, 548
AgsI TTSAA 4 cut(s) 632, 687, 739, 802
AhlI ACTAGT 1 cut(s) 367
AjnI CCWGG 2 cut(s) 252, 547
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AleI CACNNNNGTG 1 cut(s) 297
AluBI AGCT 7 cut(s) 389, 410, 440, 647, 671, 824, 847
AluI AGCT 7 cut(s) 389, 410, 440, 647, 671, 824, 847
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 1 cut(s) 804
AoxI GGCC 3 cut(s) 84, 301, 551
ApeKI GCWGC 2 cut(s) 286, 692
ApoI RAATTY 3 cut(s) 470, 566, 777
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 1 cut(s) 551
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 4 cut(s) 284, 391, 652, 910
BalI TGGCCA 1 cut(s) 303
BanI GGYRCC 1 cut(s) 354
BanII GRGCYC 2 cut(s) 277, 849
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 2 cut(s) 339, 922
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 2 cut(s) 273, 704
BccI CCATC 2 cut(s) 720, 946
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BciT130I CCWGG 2 cut(s) 254, 549
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 190
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 2 cut(s) 368, 437
BfmI CTRYAG 3 cut(s) 240, 384, 666
BfuI GTATCC 1 cut(s) 916
BisI GCNGC 2 cut(s) 287, 693
BlpI GCTNAGC 2 cut(s) 191, 876
BlsI GCNGC 2 cut(s) 288, 694
BmcAI AGTACT 1 cut(s) 366
Bme1390I CCNGG 3 cut(s) 254, 460, 549
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 2 cut(s) 276, 356
BmrFI CCNGG 3 cut(s) 254, 460, 549
BmsI GCATC 1 cut(s) 777
BpiI GAAGAC 2 cut(s) 339, 922
BpmI CTGGAG 2 cut(s) 130, 187
Bpu10I CCTNAGC 1 cut(s) 23
Bpu1102I GCTNAGC 2 cut(s) 191, 876
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 460
BsaJI CCNNGG 3 cut(s) 295, 547, 548
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 132, 171, 253, 548
Bse1I ACTGG 3 cut(s) 113, 204, 909
Bse3DI GCAATG 1 cut(s) 871
BseBI CCWGG 2 cut(s) 254, 549
BseDI CCNNGG 3 cut(s) 295, 547, 548
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 5 cut(s) 58, 132, 171, 253, 548
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 5 cut(s) 14, 205, 743, 867, 954
BseNI ACTGG 3 cut(s) 113, 204, 909
BseXI GCAGC 2 cut(s) 273, 704
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 3 cut(s) 86, 303, 553
BshNI GGYRCC 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 849
BsiSI CCGG 2 cut(s) 459, 642
BslI CCNNNNNNNGG 5 cut(s) 58, 132, 171, 253, 548
BsmAI GTCTC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 3 cut(s) 86, 303, 553
Bsp1286I GDGCHC 2 cut(s) 277, 849
Bsp143I GATC 3 cut(s) 418, 754, 809
Bsp1720I GCTNAGC 2 cut(s) 191, 876
BspACI CCGC 3 cut(s) 165, 318, 476
BspANI GGCC 3 cut(s) 86, 303, 553
BspCNI CTCAG 5 cut(s) 15, 204, 742, 868, 953
BspLI GGNNCC 2 cut(s) 276, 356
BspPI GGATC 1 cut(s) 804
BspT107I GGYRCC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 871
BsrI ACTGG 3 cut(s) 113, 204, 909
BssECI CCNNGG 3 cut(s) 295, 547, 548
BssMI GATC 3 cut(s) 418, 754, 809
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 254, 549
Bst4CI ACNGT 2 cut(s) 147, 184
BstC8I GCNNGC 2 cut(s) 153, 505
BstDEI CTNAG 7 cut(s) 23, 191, 219, 616, 729, 876, 940
BstF5I GGATG 1 cut(s) 580
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 3 cut(s) 421, 757, 812
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 3 cut(s) 418, 754, 809
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 2 cut(s) 254, 549
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 3 cut(s) 252, 458, 547
BstSFI CTRYAG 3 cut(s) 240, 384, 666
BstV1I GCAGC 2 cut(s) 273, 704
BstV2I GAAGAC 2 cut(s) 339, 922
BstX2I RGATCY 1 cut(s) 809
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 809
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 3 cut(s) 86, 303, 553
BtsCI GGATG 1 cut(s) 580
Cac8I GCNNGC 2 cut(s) 153, 505
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 551
CsiI ACCWGGT 1 cut(s) 252
Csp6I GTAC 2 cut(s) 365, 761
CviAII CATG 5 cut(s) 76, 94, 422, 571, 707
CviQI GTAC 2 cut(s) 365, 761
DdeI CTNAG 7 cut(s) 23, 191, 219, 616, 729, 876, 940
DpnI GATC 3 cut(s) 420, 756, 811
DpnII GATC 3 cut(s) 418, 754, 809
EaeI YGGCCR 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 277, 849
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 502
EcoICRI GAGCTC 1 cut(s) 847
EcoRII CCWGG 2 cut(s) 252, 547
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 2 cut(s) 277, 849
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 5 cut(s) 79, 97, 425, 574, 710
FatI CATG 5 cut(s) 75, 93, 421, 570, 706
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 287, 693
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 2 cut(s) 277, 849
Fsp4HI GCNGC 2 cut(s) 287, 693
FspBI CTAG 2 cut(s) 368, 437
GlaI GCGC 1 cut(s) 426
GluI GCNGC 2 cut(s) 287, 693
GsuI CTGGAG 2 cut(s) 130, 187
HaeIII GGCC 3 cut(s) 86, 303, 553
HapII CCGG 2 cut(s) 459, 642
HhaI GCGC 1 cut(s) 427
Hin1II CATG 5 cut(s) 79, 97, 425, 574, 710
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 3 cut(s) 231, 524, 595
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 2 cut(s) 459, 642
HphI GGTGA 4 cut(s) 284, 391, 652, 910
Hpy166II GTNNAC 2 cut(s) 244, 613
Hpy188I TCNGA 5 cut(s) 521, 775, 817, 830, 943
Hpy188III TCNNGA 1 cut(s) 802
Hpy8I GTNNAC 2 cut(s) 244, 613
HpyAV CCTTC 2 cut(s) 667, 805
HpyCH4III ACNGT 2 cut(s) 147, 184
HpyCH4V TGCA 6 cut(s) 4, 123, 179, 503, 692, 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 7 cut(s) 23, 191, 219, 616, 729, 876, 940
Hsp92II CATG 5 cut(s) 79, 97, 425, 574, 710
HspAI GCGC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 418
KspAI GTTAAC 1 cut(s) 613
Kzo9I GATC 3 cut(s) 418, 754, 809
LmnI GCTCC 3 cut(s) 280, 644, 676
Lsp1109I GCAGC 2 cut(s) 273, 704
LweI GCATC 1 cut(s) 777
MabI ACCWGGT 1 cut(s) 252
MaeI CTAG 2 cut(s) 368, 437
MaeIII GTNAC 3 cut(s) 78, 141, 379
MalI GATC 3 cut(s) 420, 756, 811
MboI GATC 3 cut(s) 418, 754, 809
MboII GAAGA 5 cut(s) 344, 442, 712, 790, 922
MflI RGATCY 1 cut(s) 809
MhlI GDGCHC 2 cut(s) 277, 849
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 5 cut(s) 214, 470, 566, 697, 777
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 1 cut(s) 533
MnlI CCTC 9 cut(s) 83, 345, 466, 601, 718, 823, 884, 931, 944
Mox20I TGGCCA 1 cut(s) 303
MscI TGGCCA 1 cut(s) 303
MseI TTAA 3 cut(s) 404, 429, 612
MslI CAYNNNNRTG 2 cut(s) 92, 297
Msp20I TGGCCA 1 cut(s) 303
MspI CCGG 2 cut(s) 459, 642
MspR9I CCNGG 3 cut(s) 254, 460, 549
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 2 cut(s) 254, 549
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 460
NdeII GATC 3 cut(s) 418, 754, 809
NlaIII CATG 5 cut(s) 79, 97, 425, 574, 710
NlaIV GGNNCC 2 cut(s) 276, 356
NmeAIII GCCGAG 2 cut(s) 242, 744
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 710
OliI CACNNNNGTG 1 cut(s) 297
PasI CCCWGGG 1 cut(s) 548
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 231, 595
PflMI CCANNNNNTGG 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 458
PkrI GCNGC 2 cut(s) 288, 694
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 252, 547
PspGI CCWGG 2 cut(s) 252, 547
PspN4I GGNNCC 2 cut(s) 276, 356
PspPI GGNCC 1 cut(s) 551
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PsuI RGATCY 1 cut(s) 809
RsaI GTAC 2 cut(s) 366, 762
RsaNI GTAC 2 cut(s) 365, 761
RseI CAYNNNNRTG 2 cut(s) 92, 297
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 3 cut(s) 404, 429, 612
SatI GCNGC 2 cut(s) 287, 693
Sau3AI GATC 3 cut(s) 418, 754, 809
Sau96I GGNCC 1 cut(s) 551
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 3 cut(s) 254, 460, 549
SduI GDGCHC 2 cut(s) 277, 849
SexAI ACCWGGT 1 cut(s) 252
SfaNI GCATC 1 cut(s) 777
SfcI CTRYAG 3 cut(s) 240, 384, 666
SmiMI CAYNNNNRTG 2 cut(s) 92, 297
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 5 cut(s) 214, 470, 566, 697, 777
SsiI CCGC 3 cut(s) 165, 318, 476
SspMI CTAG 2 cut(s) 368, 437
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 3 cut(s) 252, 458, 547
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 147, 184
TaqI TCGA 1 cut(s) 723
TasI AATT 5 cut(s) 214, 470, 566, 697, 777
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 2 cut(s) 231, 595
Tru1I TTAA 3 cut(s) 404, 429, 612
Tru9I TTAA 3 cut(s) 404, 429, 612
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 286, 692
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 3 cut(s) 559, 591, 834
TspGWI ACGGA 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 132
XapI RAATTY 3 cut(s) 470, 566, 777
XceI RCATGY 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 2 cut(s) 368, 437
ZrmI AGTACT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.