Rroxscaffold_2G00100650

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000618
Physical Location & Seq
Forward (+)
22451293 .. 22453446
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100650.1

Sequence Viewer

Length: 2154 bp
ATGCAGATTTTCCCAGAAAGTCTGAGTATATTATGGAGCTTGTGGGAAATCCGTGTTATGGTGCTTCTAAGTCTCATCTTACAAGGCATTCTCATCACGAATGGCAACCGGAGAAAGTATTCATGCAGCAACTGGCTCAGATTTCTGTGGTTTGCCTACTTGTCCGCAGATTGGGTTGCAACAGTCTCACTCAGTGTTCTTTCCAGCGATATTTCATCTGCGGACAATGGAACTAACAAATCGATAGACCCAAACAGCATCATCACAGCATTCTGGGCTCCGTTTCTCCTTCTCCACCTTGGCGGCCCAGATACCATTACCGCATACTCCTTGGAAGACAATGAGTTATGGTGGAGGCATTTGCTTGTACTAGTTGTCCAAGTATCTCTGGCTTTCTATGTATTTCTTAGGGCGTGGAGTGGCCATGCCCTGAACTTTCTAGCGATTCCAATGTTCGTTGCTGGAATTAACAAGTTTGCAGAAAGGACTTGGGTTTTGAGGTCTGCCAGCAGTAAGCACTTCAGAAAGTCAATGTTCCCTCGTCCTGATCCGGGGCCTGACTACGCCAGATACGTGGACGAGTATCGATCAAAGGCGGATGAAGGATTCAAGGTTAAGGTGGGGAAAGTAAAGGAAGCTCCCATCATAGGTGATTATTCCATTACAGCTCCCAGCAGCATTCCAAATGCAGCTGATTTGCAACACGCCCATCTTTTCTTCCAGATTTTCAAGAAGCTCTTTGCTGATCTCATTCTGAGTATCCATGATATTACGAACAGCCAATCCTTCTTCTGGAACATATCCTGTGATGAAGCTTTCAAGGTGATTGAGATTGAGCTTGGGTTTATGTATGATTTGTTTTATACCAAGGCAGCCCTTTATATAGTCAAGGCCGGTGCAATTGTTCGTTCTATCAGTTCTGTCTGCATCATTTATGTCTTTGTATCCTTCTTGACCATCAGCAAGCAAGAATACAAAGAAGCAAATGTGATCATCACTTATATGTTATTGGCTGGAGCTATCATCCTAGAGGTCTATGCTGTGTTTCAATTGCTGCGCTCGGATTGGACAATGCTATGGCTCACTAAGCACAAGAAAGCAGAGGATTTGTTGTATCCACTCATTTCATTCATTCCTTTAACTGGAGACAAAAGGTGGTCCAATGTCTTCACCAAGTACAACTTTATAAGATTTTGCCTACAAGAGAAGCCGGCCAAATATTGCCGATGTCTCCCTAAGGTGCCATGGAGATACAGAACATACAAGAACGAAGAAAAGGTTCCTAGAGAATTGAAAGAATTGATATTTCAGCAGCTTATAAAGAAATCAAGAACTGAGGATTGCAAGAAAGTATGTGCTTATAGGGGTGACTGGGTACTTGAAAAGGAGGAGGATAAATCATGCCGCAAAAAACTAGGCTGGAGCATTGGAGAAGAATTTGATCAAAGCATTCTTCTTTGGCATATTGCAACACACCTCTGCTATTACTCTGATTTAAAAAGATACTCGAACTCTTTTAGTTATCCAACTATCTCTGATCCAGAAAATAACTCAAACTCTTTCTGCCATCCAAAATGTGACGATTTTTTTGGCAAGGGTCCAAAATGTGACTCTGATCTGGAAAAATACTCAAATTGTTTCTCTGATCCAAATTGTGAAGCCAGCAAGTTGATATCAGAGTATATGTTATATCTTCTAGTCAAGCGCCCCTTCATGCTGCCCAATGGGATTGGGCAAATCAGGTTCCAGGACACATGTGCCGAAGCCATTGAATTTTTTGAACAAAGGGAATGCAACGGAGATGCAAAAGCTTGTGACGAGTTACTAGCGGTGAACACCAAGATTGATCCAGCTGAAGTAAAAGGAGATAGAAGCAAGTCTGTGTTATTCAATGCGTGCAAGCTTGCTAATGCTTTGCAGTCACTGGAAACCGAGCAAAAATGGGAGAATCAGAAGAAGTGGGAGTTGATTAGTCAAGTGTGGGTGGAAATGCTATCTTATGCGGCTAGTCAATGTCGATGGAGCCACCACGCTCGACAACTCAGGCGAGGTGGTCAGCTGATCACACATGTCTGGCTTCTTATGGCACATCTTGGCATAACTCAGCAGATTCGAACTTTCAAATTCGAAATGGCCAAGTTAGAGCACGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

717

Amino Acids

82.99

Weight (kDa)

8.48

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 50 - 397 3.5e-101 Domain of unknown function (DUF4220)
DUF594 PF04578 647 - 700 2.7e-24 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1187, 1319
AccB1I GGYRCC 1 cut(s) 1240
AciI CCGC 8 cut(s) 165, 221, 303, 321, 596, 1405, 1829, 2003
AclWI GGATC 4 cut(s) 542, 1532, 1640, 1841
AcoI YGGCCR 3 cut(s) 421, 1212, 2133
AcsI RAATTY 3 cut(s) 1436, 1772, 2123
AcuI CTGAAG 2 cut(s) 505, 1875
AdeI CACNNNGTG 1 cut(s) 194
AfaI GTAC 3 cut(s) 369, 1178, 1377
AfiI CCNNNNNNNGG 6 cut(s) 58, 171, 551, 647, 792, 1142
AflIII ACRYGT 2 cut(s) 1754, 2068
AhlI ACTAGT 1 cut(s) 370
AjnI CCWGG 1 cut(s) 1746
AloI GAACNNNNNNTCC 2 cut(s) 767, 799
Alw21I GWGCWC 1 cut(s) 2148
Alw26I GTCTC 4 cut(s) 77, 190, 1140, 1235
AlwI GGATC 4 cut(s) 542, 1532, 1640, 1841
AlwNI CAGNNNCTG 2 cut(s) 132, 1924
AoxI GGCC 6 cut(s) 304, 421, 554, 891, 1212, 2133
ApeKI GCWGC 7 cut(s) 126, 675, 689, 872, 1054, 1312, 1717
ApoI RAATTY 3 cut(s) 1436, 1772, 2123
ArsI GACNNNNNNTTYG 4 cut(s) 478, 510, 1571, 1603
Asp700I GAANNNNTTC 2 cut(s) 118, 1278
AspLEI GCGC 2 cut(s) 1059, 1707
AspS9I GGNCC 4 cut(s) 305, 554, 1158, 1598
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 5 cut(s) 662, 835, 1162, 1379, 1843
AsuII TTCGAA 2 cut(s) 2113, 2127
AvaII GGWCC 2 cut(s) 1158, 1598
AxyI CCTNAGG 1 cut(s) 1236
BalI TGGCCA 2 cut(s) 423, 2135
BanI GGYRCC 1 cut(s) 1240
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 2 cut(s) 342, 1159
Bbv12I GWGCWC 1 cut(s) 2148
BbvI GCAGC 7 cut(s) 138, 687, 701, 884, 1041, 1324, 1704
BccI CCATC 5 cut(s) 650, 717, 965, 1575, 2013
BciT130I CCWGG 1 cut(s) 1748
BciVI GTATCC 3 cut(s) 770, 955, 1125
BclI TGATCA 3 cut(s) 990, 1441, 2061
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 4 cut(s) 77, 190, 1140, 1235
BcuI ACTAGT 1 cut(s) 370
BfaI CTAG 8 cut(s) 371, 440, 1028, 1284, 1415, 1697, 1826, 2007
BfoI RGCGCY 1 cut(s) 1708
BfuI GTATCC 3 cut(s) 770, 955, 1125
Bme1390I CCNGG 2 cut(s) 552, 1748
Bme18I GGWCC 2 cut(s) 1158, 1598
BmgT120I GGNCC 4 cut(s) 305, 554, 1158, 1598
BmiI GGNNCC 7 cut(s) 279, 555, 1242, 1281, 1599, 1745, 2024
BmrFI CCNGG 2 cut(s) 552, 1748
BmrI ACTGGG 1 cut(s) 1381
BmsI GCATC 3 cut(s) 267, 936, 1792
BmuI ACTGGG 1 cut(s) 1381
BpiI GAAGAC 2 cut(s) 342, 1159
BpmI CTGGAG 3 cut(s) 1035, 1164, 1441
Bpu14I TTCGAA 2 cut(s) 2113, 2127
BpuMI CCSGG 1 cut(s) 552
Bsa29I ATCGAT 2 cut(s) 242, 586
BsaAI YACGTR 1 cut(s) 574
BsaJI CCNNGG 5 cut(s) 298, 330, 551, 867, 1244
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 171, 551, 647, 792, 1142
Bse118I RCCGGY 2 cut(s) 893, 1210
Bse1I ACTGG 4 cut(s) 137, 1147, 1376, 1929
Bse21I CCTNAGG 1 cut(s) 1236
BseBI CCWGG 1 cut(s) 1748
BseCI ATCGAT 2 cut(s) 242, 586
BseDI CCNNGG 5 cut(s) 298, 330, 551, 867, 1244
BseGI GGATG 3 cut(s) 604, 1023, 1567
BseLI CCNNNNNNNGG 6 cut(s) 58, 171, 551, 647, 792, 1142
BseMII CTCAG 7 cut(s) 14, 151, 205, 746, 1326, 2056, 2117
BseNI ACTGG 4 cut(s) 137, 1147, 1376, 1929
BseRI GAGGAG 1 cut(s) 1403
BseXI GCAGC 7 cut(s) 138, 687, 701, 884, 1041, 1324, 1704
BseYI CCCAGC 1 cut(s) 671
BshFI GGCC 6 cut(s) 306, 423, 556, 893, 1214, 2135
BshNI GGYRCC 1 cut(s) 1240
BshVI ATCGAT 2 cut(s) 242, 586
BsiHKAI GWGCWC 1 cut(s) 2148
BsiSI CCGG 4 cut(s) 109, 551, 894, 1211
BslI CCNNNNNNNGG 6 cut(s) 58, 171, 551, 647, 792, 1142
BsmAI GTCTC 4 cut(s) 77, 190, 1140, 1235
BsmI GAATGC 5 cut(s) 87, 269, 678, 1449, 1796
BsnI GGCC 6 cut(s) 306, 423, 556, 893, 1214, 2135
Bsp119I TTCGAA 2 cut(s) 2113, 2127
Bsp1286I GDGCHC 2 cut(s) 280, 2148
Bsp19I CCATGG 1 cut(s) 1244
BspACI CCGC 8 cut(s) 165, 221, 303, 321, 596, 1405, 1829, 2003
BspANI GGCC 6 cut(s) 306, 423, 556, 893, 1214, 2135
BspCNI CTCAG 7 cut(s) 15, 150, 204, 747, 1327, 2055, 2116
BspDI ATCGAT 2 cut(s) 242, 586
BspLI GGNNCC 7 cut(s) 279, 555, 1242, 1281, 1599, 1745, 2024
BspPI GGATC 4 cut(s) 542, 1532, 1640, 1841
BspT104I TTCGAA 2 cut(s) 2113, 2127
BspT107I GGYRCC 1 cut(s) 1240
BsrFI RCCGGY 2 cut(s) 893, 1210
BsrI ACTGG 4 cut(s) 137, 1147, 1376, 1929
BssAI RCCGGY 2 cut(s) 893, 1210
BssECI CCNNGG 5 cut(s) 298, 330, 551, 867, 1244
BssT1I CCWWGG 4 cut(s) 298, 330, 867, 1244
Bst2UI CCWGG 1 cut(s) 1748
Bst4CI ACNGT 2 cut(s) 184, 2150
BstBAI YACGTR 1 cut(s) 574
BstBI TTCGAA 2 cut(s) 2113, 2127
BstC8I GCNNGC 7 cut(s) 508, 965, 1212, 1663, 1897, 1901, 1905
BstDSI CCRYGG 1 cut(s) 1244
BstF5I GGATG 3 cut(s) 604, 1023, 1567
BstH2I RGCGCY 1 cut(s) 1708
BstHHI GCGC 2 cut(s) 1059, 1707
BstMAI GTCTC 4 cut(s) 77, 190, 1140, 1235
BstMWI GCNNNNNNNGC 2 cut(s) 275, 1087
BstNI CCWGG 1 cut(s) 1748
BstNSI RCATGY 2 cut(s) 1758, 2072
BstSCI CCNGG 2 cut(s) 550, 1746
BstV1I GCAGC 7 cut(s) 138, 687, 701, 884, 1041, 1324, 1704
BstV2I GAAGAC 2 cut(s) 342, 1159
BstXI CCANNNNNNTGG 1 cut(s) 574
Bsu15I ATCGAT 2 cut(s) 242, 586
Bsu36I CCTNAGG 1 cut(s) 1236
BsuI GTATCC 3 cut(s) 770, 955, 1125
BsuRI GGCC 6 cut(s) 306, 423, 556, 893, 1214, 2135
BsuTUI ATCGAT 2 cut(s) 242, 586
BtgI CCRYGG 1 cut(s) 1244
BtsCI GGATG 3 cut(s) 604, 1023, 1567
BtsIMutI CAGTG 2 cut(s) 199, 1922
Cac8I GCNNGC 7 cut(s) 508, 965, 1212, 1663, 1897, 1901, 1905
CaiI CAGNNNCTG 2 cut(s) 132, 1924
CfoI GCGC 2 cut(s) 1059, 1707
Cfr10I RCCGGY 2 cut(s) 893, 1210
Cfr13I GGNCC 4 cut(s) 305, 554, 1158, 1598
ClaI ATCGAT 2 cut(s) 242, 586
Csp6I GTAC 3 cut(s) 368, 1177, 1376
CviAII CATG 8 cut(s) 123, 425, 764, 1245, 1401, 1714, 1755, 2069
CviQI GTAC 3 cut(s) 368, 1177, 1376
DraI TTTAAA 1 cut(s) 1497
DraIII CACNNNGTG 1 cut(s) 194
EaeI YGGCCR 3 cut(s) 421, 1212, 2133
EciI GGCGGA 1 cut(s) 611
Eco130I CCWWGG 4 cut(s) 298, 330, 867, 1244
Eco24I GRGCYC 1 cut(s) 280
Eco32I GATATC 1 cut(s) 1674
Eco47I GGWCC 2 cut(s) 1158, 1598
Eco57I CTGAAG 2 cut(s) 505, 1875
Eco81I CCTNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 1746
EcoRV GATATC 1 cut(s) 1674
EcoT14I CCWWGG 4 cut(s) 298, 330, 867, 1244
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 4 cut(s) 298, 330, 867, 1244
FaeI CATG 8 cut(s) 126, 428, 767, 1248, 1404, 1717, 1758, 2072
FalI AAGNNNNNCTT 6 cut(s) 722, 754, 1166, 1198, 1694, 1726
FatI CATG 8 cut(s) 122, 424, 763, 1244, 1400, 1713, 1754, 2068
FbaI TGATCA 3 cut(s) 990, 1441, 2061
FokI GGATG 3 cut(s) 611, 1010, 1554
FriOI GRGCYC 1 cut(s) 280
FspBI CTAG 8 cut(s) 371, 440, 1028, 1284, 1415, 1697, 1826, 2007
GlaI GCGC 2 cut(s) 1058, 1706
GsaI CCCAGC 1 cut(s) 675
GsuI CTGGAG 3 cut(s) 1035, 1164, 1441
HaeII RGCGCY 1 cut(s) 1708
HaeIII GGCC 6 cut(s) 306, 423, 556, 893, 1214, 2135
HapII CCGG 4 cut(s) 109, 551, 894, 1211
HhaI GCGC 2 cut(s) 1059, 1707
Hin1II CATG 8 cut(s) 126, 428, 767, 1248, 1404, 1717, 1758, 2072
Hin6I GCGC 2 cut(s) 1057, 1705
HinP1I GCGC 2 cut(s) 1057, 1705
HindIII AAGCTT 3 cut(s) 813, 1809, 1901
HinfI GANTC 5 cut(s) 445, 606, 1610, 1948, 2110
HpaII CCGG 4 cut(s) 109, 551, 894, 1211
HphI GGTGA 5 cut(s) 662, 835, 1162, 1379, 1843
Hpy166II GTNNAC 2 cut(s) 577, 1834
Hpy188III TCNNGA 9 cut(s) 97, 545, 721, 730, 793, 952, 1329, 1541, 1619
Hpy8I GTNNAC 2 cut(s) 577, 1834
HpyAV CCTTC 5 cut(s) 299, 596, 796, 958, 1720
HpyCH4III ACNGT 2 cut(s) 184, 2150
HpyCH4IV ACGT 1 cut(s) 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 1087
HpySE526I ACGT 1 cut(s) 573
Hsp92II CATG 8 cut(s) 126, 428, 767, 1248, 1404, 1717, 1758, 2072
HspAI GCGC 2 cut(s) 1057, 1705
KroI GCCGGC 1 cut(s) 1210
KroNI GCCGGC 1 cut(s) 1212
Ksp22I TGATCA 3 cut(s) 990, 1441, 2061
LmnI GCTCC 7 cut(s) 36, 283, 643, 673, 1016, 1422, 2022
Lsp1109I GCAGC 7 cut(s) 138, 687, 701, 884, 1041, 1324, 1704
LweI GCATC 3 cut(s) 267, 936, 1792
MaeI CTAG 8 cut(s) 371, 440, 1028, 1284, 1415, 1697, 1826, 2007
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 6 cut(s) 1367, 1577, 1607, 1814, 1821, 1920
MboII GAAGA 9 cut(s) 347, 709, 781, 1159, 1283, 1445, 1445, 1685, 1966
MfeI CAATTG 2 cut(s) 900, 1049
MhlI GDGCHC 2 cut(s) 280, 2148
MlsI TGGCCA 2 cut(s) 423, 2135
MluNI TGGCCA 2 cut(s) 423, 2135
MlyI GAGTC 1 cut(s) 1604
MmeI TCCRAC 1 cut(s) 1550
Mox20I TGGCCA 2 cut(s) 423, 2135
MroNI GCCGGC 1 cut(s) 1210
MroXI GAANNNNTTC 2 cut(s) 118, 1278
MscI TGGCCA 2 cut(s) 423, 2135
MseI TTAA 4 cut(s) 468, 615, 1139, 1496
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 423, 2135
MspA1I CMGCKG 3 cut(s) 692, 1853, 2059
MspI CCGG 4 cut(s) 109, 551, 894, 1211
MspR9I CCNGG 2 cut(s) 552, 1748
MunI CAATTG 2 cut(s) 900, 1049
Mva1269I GAATGC 5 cut(s) 87, 269, 678, 1449, 1796
MvaI CCWGG 1 cut(s) 1748
MwoI GCNNNNNNNGC 2 cut(s) 275, 1087
NaeI GCCGGC 1 cut(s) 1212
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 1 cut(s) 1244
NgoMIV GCCGGC 1 cut(s) 1210
NlaIII CATG 8 cut(s) 126, 428, 767, 1248, 1404, 1717, 1758, 2072
NlaIV GGNNCC 7 cut(s) 279, 555, 1242, 1281, 1599, 1745, 2024
NmuCI GTSAC 5 cut(s) 1367, 1577, 1607, 1814, 1920
NspI RCATGY 2 cut(s) 1758, 2072
NspV TTCGAA 2 cut(s) 2113, 2127
PciI ACATGT 2 cut(s) 1754, 2068
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 5 cut(s) 87, 269, 678, 1449, 1796
PdiI GCCGGC 1 cut(s) 1212
PdmI GAANNNNTTC 2 cut(s) 118, 1278
PfeI GAWTC 4 cut(s) 445, 606, 1948, 2110
PfoI TCCNGGA 1 cut(s) 1746
PleI GAGTC 1 cut(s) 1604
PpsI GAGTC 1 cut(s) 1604
Ppu21I YACGTR 1 cut(s) 574
PscI ACATGT 2 cut(s) 1754, 2068
PsiI TTATAA 2 cut(s) 1187, 1319
Psp6I CCWGG 1 cut(s) 1746
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 1 cut(s) 1746
PspN4I GGNNCC 7 cut(s) 279, 555, 1242, 1281, 1599, 1745, 2024
PspPI GGNCC 4 cut(s) 305, 554, 1158, 1598
PstNI CAGNNNCTG 2 cut(s) 132, 1924
PvuII CAGCTG 3 cut(s) 692, 1853, 2059
RsaI GTAC 3 cut(s) 369, 1178, 1377
RsaNI GTAC 3 cut(s) 368, 1177, 1376
RseI CAYNNNNRTG 1 cut(s) 1001
SaqAI TTAA 4 cut(s) 468, 615, 1139, 1496
Sau96I GGNCC 4 cut(s) 305, 554, 1158, 1598
SchI GAGTC 1 cut(s) 1604
ScrFI CCNGG 2 cut(s) 552, 1748
SduI GDGCHC 2 cut(s) 280, 2148
SfaNI GCATC 3 cut(s) 267, 936, 1792
SfuI TTCGAA 2 cut(s) 2113, 2127
SinI GGWCC 2 cut(s) 1158, 1598
SmiMI CAYNNNNRTG 1 cut(s) 1001
SpeI ACTAGT 1 cut(s) 370
SsiI CCGC 8 cut(s) 165, 221, 303, 321, 596, 1405, 1829, 2003
SspI AATATT 1 cut(s) 1220
SspMI CTAG 8 cut(s) 371, 440, 1028, 1284, 1415, 1697, 1826, 2007
StyD4I CCNGG 2 cut(s) 550, 1746
StyI CCWWGG 4 cut(s) 298, 330, 867, 1244
TaaI ACNGT 2 cut(s) 184, 2150
TaiI ACGT 1 cut(s) 576
TaqI TCGA 7 cut(s) 242, 586, 1508, 2017, 2035, 2113, 2127
TatI WGTACW 2 cut(s) 367, 1176
TauI GCSGC 3 cut(s) 306, 1407, 2006
TfiI GAWTC 4 cut(s) 445, 606, 1948, 2110
Tru1I TTAA 4 cut(s) 468, 615, 1139, 1496
Tru9I TTAA 4 cut(s) 468, 615, 1139, 1496
TscAI CASTG 2 cut(s) 199, 1929
TseFI GTSAC 5 cut(s) 1367, 1577, 1607, 1814, 1920
TseI GCWGC 7 cut(s) 126, 675, 689, 872, 1054, 1312, 1717
Tsp45I GTSAC 5 cut(s) 1367, 1577, 1607, 1814, 1920
TspDTI ATGAA 7 cut(s) 111, 204, 615, 825, 1116, 1120, 1702
TspGWI ACGGA 3 cut(s) 41, 270, 1812
TspRI CASTG 2 cut(s) 199, 1929
VpaK11BI GGWCC 2 cut(s) 1158, 1598
XapI RAATTY 3 cut(s) 1436, 1772, 2123
XceI RCATGY 2 cut(s) 1758, 2072
XmnI GAANNNNTTC 2 cut(s) 118, 1278
XspI CTAG 8 cut(s) 371, 440, 1028, 1284, 1415, 1697, 1826, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.