Rh2BG457300

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000618
Physical Location & Seq
Reverse (-)
64731357 .. 64756539
25183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG457300.1

Sequence Viewer

Length: 312 bp
ATGAACTCAACAAATTTACTATTATTCCTACTTCCCAGCAATTCGATAGACCATGATGCGAATTCAAAAGACCTAGACTACATCATCATAGCATTTTGGGCTCCATTTCTTCTTCTTCACCTCGGTGGGCCAGACACCATTACCGCGTCATCTTTGGAAGACAATGAGTTGTGGTCGAGGCACTTCCTTGTGCTACTAGTCCAGGTTGCAGTCGCTTTCTACATCTACCTTAGAGCCTGGGGTGGTTACGCCAGATACGTTAGGCATTATCAAATTTGGAGAAAGTACTTGGGTTCTGAGGTCTGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

103

Amino Acids

11.95

Weight (kDa)

6.38

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 22 - 82 2.3e-25 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 146
AciI CCGC 1 cut(s) 144
AcsI RAATTY 3 cut(s) 13, 61, 273
AfaI GTAC 1 cut(s) 287
AgsI TTSAA 1 cut(s) 66
AhlI ACTAGT 1 cut(s) 196
AjnI CCWGG 2 cut(s) 201, 236
AleI CACNNNNGTG 1 cut(s) 123
AoxI GGCC 1 cut(s) 128
ApoI RAATTY 3 cut(s) 13, 61, 273
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 110
BanII GRGCYC 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 165
BciT130I CCWGG 2 cut(s) 203, 238
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 2 cut(s) 74, 197
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 2 cut(s) 203, 238
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 203, 238
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 121, 237
BseBI CCWGG 2 cut(s) 203, 238
BseDI CCNNGG 2 cut(s) 121, 237
BseMII CTCAG 1 cut(s) 288
BseYI CCCAGC 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 130
BsnI GGCC 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 103
BspACI CCGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 121, 237
Bst2UI CCWGG 2 cut(s) 203, 238
BstDEI CTNAG 2 cut(s) 230, 297
BstFNI CGCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 2 cut(s) 203, 238
BstSCI CCNGG 2 cut(s) 201, 236
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 128
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 286
CviAII CATG 1 cut(s) 53
CviJI RGCY 3 cut(s) 101, 130, 236
CviKI_1 RGCY 3 cut(s) 101, 130, 236
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 2 cut(s) 230, 297
Eco24I GRGCYC 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 2 cut(s) 201, 236
EcoT38I GRGCYC 1 cut(s) 103
FaeI CATG 1 cut(s) 56
FaiI YATR 2 cut(s) 54, 89
FatI CATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 103
FspBI CTAG 2 cut(s) 74, 197
GsaI CCCAGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 130
HgaI GACGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 56
HphI GGTGA 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 209, 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 230, 297
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 1 cut(s) 56
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 7 cut(s) 49, 144, 188, 215, 223, 250, 265
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 74, 197
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 4 cut(s) 101, 104, 107, 170
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 4 cut(s) 13, 40, 61, 273
MnlI CCTC 3 cut(s) 131, 171, 292
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 203, 238
MvaI CCWGG 2 cut(s) 203, 238
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 98
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 1 cut(s) 102
OliI CACNNNNGTG 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 201, 236
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 2 cut(s) 201, 236
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 128
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 128
ScaI AGTACT 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 203, 238
SduI GDGCHC 1 cut(s) 103
SetI ASST 6 cut(s) 75, 123, 207, 231, 261, 303
SfaNI GCATC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 123
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 4 cut(s) 13, 40, 61, 273
SsiI CCGC 1 cut(s) 144
SspMI CTAG 2 cut(s) 74, 197
StyD4I CCNGG 2 cut(s) 201, 236
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 44, 176
TasI AATT 4 cut(s) 13, 40, 61, 273
TatI WGTACW 1 cut(s) 285
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 3 cut(s) 13, 61, 273
XspI CTAG 2 cut(s) 74, 197
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.