MD17G1025500.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000618
Physical Location & Seq
Forward (+)
1854874 .. 1857369
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1025500.v1.1.491

Sequence Viewer

Length: 2142 bp
ATGTTACTCAGAGTTGTAAGCAGTTTGATCTTCACACAGAAAAGGAAGGCAATGCAAATTTTCCCCGAAAGCGTGAGAGAAGCGTGGAACGAGTGGGAGCTTCGGGCCATGGTTTTAATCAGCCTTGCCTTGCAAACCATCCTCATCCTGATCGGCAACTGGAGAAAGCATTCAACCAGCAACATACTCCGAATTGTCCTCTGGCTCGCTTACTTGTCAGCTGACTCCGTAGCAACCGTCTCACTCGGGATTCTCTCCAGCAACCAGGAAGACTCCGAAGGTGATTCTGTAAACCCCAACTTTGTTATAACAGCATTCTGGGCACCGTTTCTTCTCTTGCATCTCGGCGGTCCAGATACGATTACTGCTTACTCGTTGGAGGATAATGAGTTGTGGCCTAGGCACTTGCTTGGGGTAGTTGTCCAGGTGATTGTAGCTTTGTATGTGTTTATAAGAGCATGGTCTAGTAAAATGTTGAATTTTTTGGCAATTCCCGTTTTCGTAGTTGGAATTATAAAATTTGGGGAGAGGACTTGGGTTTTGAGGTCTGCAAGTAGTGAGCATTTTCGAGAGTCTATGCTCCCTCCTCCGGACGCAGGTCCGAATTATGCTAGATACATGGAAGAATACAGCTCAAAGAAAGCTGAGGGGTTTAGGGTTGAACCGAAAACATTCATTGAAGCTCCCATAGGTTTTGATCATTCCGATGCAGTTCAAGCTAATGAATCTAGTAGTGCCCAAAATGCTGCAGTTTTAAACAAAGCTTATAGTTTCTTCGAAACTTTCAAGCGGCTGTGTGCCGATCTCATCCTCACCTTCCACGATATAGAAAACAGCCAATCGTTCTTCCAACATAGAAGCTCTGAGGAAGCTCTCGAGGTTATTGAGTTCGAGCTAGGGTTCATGTATGATGTGTTTTATACTAAATCACTTCTGGTTTATTCTGGTCTTGGTGGAATACTCCGTTGCATTACTCTTTCTTTCACAGTTTGTGTGTTCCTGGCCTTCGTGTTGATCACGGAGAAGCAAGAATACATGAAAGTGGATGTAATTATCACATACATATTGTTGGTAGGAGCTATTGTCTTAGAATTTTATGCAGTTGTCATCCTACTTTCATCGGACTGGACCCTGCTGTGGCTGAGCAAGCACAAAAACACAGCAGTAGATCTCTTGCTCAGATTGGTTTCATCAATCGCTTTGATAAAAAACAAGAGGTGGTCTAATACCTTGGCACAATACGACCTCATAACATTTTGTCTCAAAGAAAGGCCAGCAAAGTGCATTTTCATCAACGAGGACTGGTTTATAAATCGGTTACTAGAGAAGTATCGATACAGAGACTTGAAGGATGTTTCCAAAGAGTTGAAGAATTTGATATTTGGGCAGCTGCTAGAGAAGTCGAGGACTGCTTCTAATTTCGAGGCCAGCAAACGAAAAGAACTATGTGCTCGAAGGGGTGACATGGTGCTTGAGAAAGCAAAATGTCTAGATGAACTTGGCTGGACAATTAACGGGGCCGAATTTGATCAAAGCATCCTTCTTTGGCATATTGCGACAAATCTATGTTACTATTCTGATCTGGAATTTTATCATAGTCCAAATTCTGAAGCCAGCATTCTTAAAAAAAATTCTGAAGCCAGCAAGGTGTTATCAAACTATATGTTGTATCTTTTGGTGATGTGCCCCTTCATGCTGCCAAATGGGATTGGACAGATAAGGTTTAGAGACACCTGTGCCGAGGCTGAGGAATTTATCACCGAAAGAAAGTCCATAAAGAATAGAGAAACGGCCTGCAAAATGTTGCTCAAAGTGAGCACAGACATCCTTCCATCGAAAGTGAAAGGAGATAGAAGCAAGTCGGTTCTATTTGATGCATGCAGGCTTGCTAAAGCTCTGCAGTTCCTTGAGTCTAAGAAACACTGGGAGAACAATGAGAAAAAATGGGAGTTTATAAGTCATGTGTGGGTGGAAATGCTTGCTTTCGCTGCCAATCAGTGCCGGTGGAGTGATCATGCTCAGCAGCTCAGGCGAGGAGGAGAGCTGCTCACTCATATCTGGCTTCTTATGGCACATCTTGGTTTAACTGAACAGTTTCAAATTTCAGAGGGCCATGTTAGGGCAAAGTTGATTGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

714

Amino Acids

81.89

Weight (kDa)

6.97

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 68 - 418 1.9e-109 Domain of unknown function (DUF4220)
DUF594 PF04578 643 - 695 4.9e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 308, 452, 515, 1310, 1955
Acc36I ACCTGC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 2 cut(s) 348, 790
AcuI CTGAAG 2 cut(s) 1629, 1656
AfiI CCNNNNNNNGG 4 cut(s) 589, 596, 1137, 2119
AgsI TTSAA 9 cut(s) 174, 478, 662, 680, 716, 787, 1348, 1369, 2099
AjnI CCWGG 3 cut(s) 264, 423, 999
Alw21I GWGCWC 2 cut(s) 1453, 1820
Alw26I GTCTC 4 cut(s) 244, 1265, 1335, 1722
Ama87I CYCGRG 2 cut(s) 245, 875
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 8 cut(s) 105, 395, 1002, 1271, 1425, 1518, 1791, 2110
ApeKI GCWGC 7 cut(s) 746, 1387, 1390, 1696, 1988, 2023, 2044
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
Asp700I GAANNNNTTC 3 cut(s) 169, 671, 2094
AspA2I CCTAGG 1 cut(s) 398
AspS9I GGNCC 6 cut(s) 105, 350, 599, 1128, 1518, 2110
AsuHPI GGTGA 6 cut(s) 293, 439, 805, 1472, 1690, 1750
AsuII TTCGAA 1 cut(s) 777
AvaI CYCGRG 2 cut(s) 245, 875
AvaII GGWCC 3 cut(s) 350, 599, 1128
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 3 cut(s) 325, 739, 1688
BanI GGYRCC 1 cut(s) 322
BarI GAAGNNNNNNTAC 4 cut(s) 1016, 1048, 1319, 1351
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 1453, 1820
BbvCI CCTCAGC 2 cut(s) 645, 1746
BbvI GCAGC 7 cut(s) 733, 1377, 1399, 1683, 1975, 2031, 2035
BccI CCATC 2 cut(s) 146, 1840
BceAI ACGGC 1 cut(s) 1806
BciT130I CCWGG 3 cut(s) 266, 425, 1001
BclI TGATCA 4 cut(s) 697, 1014, 1528, 2011
BcoDI GTCTC 4 cut(s) 244, 1265, 1335, 1722
BfaI CTAG 8 cut(s) 399, 465, 612, 729, 896, 1322, 1394, 1490
BfmI CTRYAG 2 cut(s) 747, 1898
BfuAI ACCTGC 1 cut(s) 587
BglII AGATCT 1 cut(s) 1168
BisI GCNGC 8 cut(s) 747, 791, 1388, 1391, 1697, 1989, 2024, 2045
BlnI CCTAGG 1 cut(s) 398
BlpI GCTNAGC 2 cut(s) 1142, 2019
BlsI GCNGC 8 cut(s) 748, 792, 1389, 1392, 1698, 1990, 2025, 2046
Bme1390I CCNGG 3 cut(s) 266, 425, 1001
Bme18I GGWCC 3 cut(s) 350, 599, 1128
BmeT110I CYCGRG 2 cut(s) 245, 875
BmgT120I GGNCC 6 cut(s) 105, 350, 599, 1128, 1518, 2110
BmiI GGNNCC 3 cut(s) 324, 1130, 1519
BmrFI CCNGG 3 cut(s) 266, 425, 1001
BmrI ACTGGG 1 cut(s) 1933
BmsI GCATC 4 cut(s) 349, 697, 1545, 1864
BmuI ACTGGG 1 cut(s) 1933
BoxI GACNNNNGTC 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 2031, 2063
BpmI CTGGAG 2 cut(s) 181, 241
Bpu10I CCTNAGC 3 cut(s) 645, 1746, 2027
Bpu1102I GCTNAGC 2 cut(s) 1142, 2019
Bpu14I TTCGAA 1 cut(s) 777
BpuEI CTTGAG 2 cut(s) 1493, 1928
Bsa29I ATCGAT 1 cut(s) 1333
BsaJI CCNNGG 4 cut(s) 108, 398, 1230, 1740
BsaWI WCCGGW 1 cut(s) 589
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 4 cut(s) 589, 596, 1137, 2119
Bse118I RCCGGY 1 cut(s) 2001
Bse1I ACTGG 4 cut(s) 164, 1130, 1307, 1928
Bse3DI GCAATG 1 cut(s) 57
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 3 cut(s) 266, 425, 1001
BseCI ATCGAT 1 cut(s) 1333
BseDI CCNNGG 4 cut(s) 108, 398, 1230, 1740
BseGI GGATG 8 cut(s) 138, 144, 807, 1051, 1107, 1357, 1536, 1824
BseLI CCNNNNNNNGG 4 cut(s) 589, 596, 1137, 2119
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 8 cut(s) 22, 636, 855, 1133, 1192, 1737, 2033, 2041
BseNI ACTGG 4 cut(s) 164, 1130, 1307, 1928
BseRI GAGGAG 3 cut(s) 576, 2049, 2052
BseSI GKGCMC 3 cut(s) 325, 739, 1688
BseXI GCAGC 7 cut(s) 733, 1377, 1399, 1683, 1975, 2031, 2035
BshFI GGCC 8 cut(s) 107, 397, 1004, 1273, 1427, 1520, 1793, 2112
BshNI GGYRCC 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 1333
BsiHKAI GWGCWC 2 cut(s) 1453, 1820
BsiHKCI CYCGRG 2 cut(s) 245, 875
BsiSI CCGG 2 cut(s) 590, 2002
BslI CCNNNNNNNGG 4 cut(s) 589, 596, 1137, 2119
BsmAI GTCTC 4 cut(s) 244, 1265, 1335, 1722
BsmBI CGTCTC 1 cut(s) 244
BsmI GAATGC 3 cut(s) 169, 314, 1617
BsnI GGCC 8 cut(s) 107, 397, 1004, 1273, 1427, 1520, 1793, 2112
BsoBI CYCGRG 2 cut(s) 245, 875
Bsp119I TTCGAA 1 cut(s) 777
Bsp1286I GDGCHC 5 cut(s) 325, 739, 1453, 1688, 1820
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
Bsp1720I GCTNAGC 2 cut(s) 1142, 2019
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 2 cut(s) 348, 790
BspANI GGCC 8 cut(s) 107, 397, 1004, 1273, 1427, 1520, 1793, 2112
BspCNI CTCAG 8 cut(s) 21, 637, 856, 1134, 1191, 1738, 2032, 2040
BspDI ATCGAT 1 cut(s) 1333
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 324, 1130, 1519
BspMAI CTGCAG 2 cut(s) 751, 1902
BspMI ACCTGC 1 cut(s) 587
BspT104I TTCGAA 1 cut(s) 777
BspT107I GGYRCC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 2001
BsrI ACTGG 4 cut(s) 164, 1130, 1307, 1928
BssAI RCCGGY 1 cut(s) 2001
BssECI CCNNGG 4 cut(s) 108, 398, 1230, 1740
BssMI GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
BssT1I CCWWGG 3 cut(s) 108, 398, 1230
Bst2UI CCWGG 3 cut(s) 266, 425, 1001
Bst4CI ACNGT 4 cut(s) 238, 327, 988, 2094
BstBI TTCGAA 1 cut(s) 777
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 8 cut(s) 138, 144, 807, 1051, 1107, 1357, 1536, 1824
BstKTI GATC 9 cut(s) 30, 153, 700, 805, 1017, 1171, 1531, 1582, 2014
BstMAI GTCTC 4 cut(s) 244, 1265, 1335, 1722
BstMBI GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
BstMWI GCNNNNNNNGC 6 cut(s) 320, 716, 743, 1147, 1988, 2029
BstNI CCWGG 3 cut(s) 266, 425, 1001
BstNSI RCATGY 1 cut(s) 1881
BstPAI GACNNNNGTC 1 cut(s) 597
BstSCI CCNGG 3 cut(s) 264, 423, 999
BstSFI CTRYAG 2 cut(s) 747, 1898
BstSLI GKGCMC 3 cut(s) 325, 739, 1688
BstV1I GCAGC 7 cut(s) 733, 1377, 1399, 1683, 1975, 2031, 2035
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 1168
Bsu15I ATCGAT 1 cut(s) 1333
BsuRI GGCC 8 cut(s) 107, 397, 1004, 1273, 1427, 1520, 1793, 2112
BsuTUI ATCGAT 1 cut(s) 1333
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 8 cut(s) 138, 144, 807, 1051, 1107, 1357, 1536, 1824
BtsIMutI CAGTG 2 cut(s) 1921, 2003
BveI ACCTGC 1 cut(s) 587
Cfr10I RCCGGY 1 cut(s) 2001
Cfr13I GGNCC 6 cut(s) 105, 350, 599, 1128, 1518, 2110
ClaI ATCGAT 1 cut(s) 1333
CseI GACGC 1 cut(s) 602
DpnI GATC 9 cut(s) 29, 152, 699, 804, 1016, 1170, 1530, 1581, 2013
DpnII GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
DraI TTTAAA 1 cut(s) 756
Eco130I CCWWGG 3 cut(s) 108, 398, 1230
Eco47I GGWCC 3 cut(s) 350, 599, 1128
Eco57I CTGAAG 2 cut(s) 1629, 1656
Eco88I CYCGRG 2 cut(s) 245, 875
EcoRII CCWGG 3 cut(s) 264, 423, 999
EcoT14I CCWWGG 3 cut(s) 108, 398, 1230
EcoT22I ATGCAT 1 cut(s) 1879
ErhI CCWWGG 3 cut(s) 108, 398, 1230
Esp3I CGTCTC 1 cut(s) 244
FbaI TGATCA 4 cut(s) 697, 1014, 1528, 2011
Fnu4HI GCNGC 8 cut(s) 747, 791, 1388, 1391, 1697, 1989, 2024, 2045
FokI GGATG 8 cut(s) 125, 131, 794, 1058, 1094, 1364, 1523, 1811
Fsp4HI GCNGC 8 cut(s) 747, 791, 1388, 1391, 1697, 1989, 2024, 2045
FspBI CTAG 8 cut(s) 399, 465, 612, 729, 896, 1322, 1394, 1490
GluI GCNGC 8 cut(s) 747, 791, 1388, 1391, 1697, 1989, 2024, 2045
GsuI CTGGAG 2 cut(s) 181, 241
HaeIII GGCC 8 cut(s) 107, 397, 1004, 1273, 1427, 1520, 1793, 2112
HapII CCGG 2 cut(s) 590, 2002
HgaI GACGC 1 cut(s) 602
HindIII AAGCTT 1 cut(s) 762
HinfI GANTC 7 cut(s) 224, 250, 272, 284, 572, 725, 1910
HpaII CCGG 2 cut(s) 590, 2002
HphI GGTGA 6 cut(s) 293, 439, 805, 1472, 1690, 1750
Hpy166II GTNNAC 1 cut(s) 292
Hpy188III TCNNGA 8 cut(s) 148, 247, 353, 569, 590, 875, 1490, 1583
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 9 cut(s) 40, 272, 826, 1015, 1342, 1449, 1550, 1699, 1838
HpyCH4III ACNGT 4 cut(s) 238, 327, 988, 2094
HpyF10VI GCNNNNNNNGC 6 cut(s) 320, 716, 743, 1147, 1988, 2029
Kpn2I TCCGGA 1 cut(s) 589
Ksp22I TGATCA 4 cut(s) 697, 1014, 1528, 2011
Kzo9I GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
LmnI GCTCC 4 cut(s) 97, 585, 688, 1076
Lsp1109I GCAGC 7 cut(s) 733, 1377, 1399, 1683, 1975, 2031, 2035
LweI GCATC 4 cut(s) 349, 697, 1545, 1864
MaeI CTAG 8 cut(s) 399, 465, 612, 729, 896, 1322, 1394, 1490
MaeIII GTNAC 4 cut(s) 3, 1317, 1460, 1568
MalI GATC 9 cut(s) 29, 152, 699, 804, 1016, 1170, 1530, 1581, 2013
MboI GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
MboII GAAGA 7 cut(s) 22, 281, 323, 635, 766, 838, 1381
MflI RGATCY 1 cut(s) 1168
MhlI GDGCHC 5 cut(s) 325, 739, 1453, 1688, 1820
MlyI GAGTC 4 cut(s) 218, 266, 581, 1919
MmeI TCCRAC 3 cut(s) 357, 487, 874
Mph1103I ATGCAT 1 cut(s) 1879
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 3 cut(s) 169, 671, 2094
MseI TTAA 5 cut(s) 116, 755, 1512, 1623, 2084
MslI CAYNNNNRTG 2 cut(s) 705, 1040
MspA1I CMGCKG 2 cut(s) 221, 1390
MspI CCGG 2 cut(s) 590, 2002
MspR9I CCNGG 3 cut(s) 266, 425, 1001
Mva1269I GAATGC 3 cut(s) 169, 314, 1617
MvaI CCWGG 3 cut(s) 266, 425, 1001
MwoI GCNNNNNNNGC 6 cut(s) 320, 716, 743, 1147, 1988, 2029
NcoI CCATGG 1 cut(s) 108
NdeII GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
NlaIV GGNNCC 3 cut(s) 324, 1130, 1519
NmeAIII GCCGAG 2 cut(s) 324, 1765
NmuCI GTSAC 1 cut(s) 1460
NsiI ATGCAT 1 cut(s) 1879
NspI RCATGY 1 cut(s) 1881
NspV TTCGAA 1 cut(s) 777
PaeI GCATGC 1 cut(s) 1881
PaeR7I CTCGAG 1 cut(s) 875
PctI GAATGC 3 cut(s) 169, 314, 1617
PdmI GAANNNNTTC 3 cut(s) 169, 671, 2094
PfeI GAWTC 3 cut(s) 250, 284, 725
PkrI GCNGC 8 cut(s) 748, 792, 1389, 1392, 1698, 1990, 2025, 2046
PleI GAGTC 4 cut(s) 218, 266, 580, 1918
PpsI GAGTC 4 cut(s) 218, 266, 580, 1918
PshAI GACNNNNGTC 1 cut(s) 597
PsiI TTATAA 5 cut(s) 308, 452, 515, 1310, 1955
Psp6I CCWGG 3 cut(s) 264, 423, 999
PspGI CCWGG 3 cut(s) 264, 423, 999
PspN4I GGNNCC 3 cut(s) 324, 1130, 1519
PspPI GGNCC 6 cut(s) 105, 350, 599, 1128, 1518, 2110
PstI CTGCAG 2 cut(s) 751, 1902
PsuI RGATCY 1 cut(s) 1168
PvuII CAGCTG 2 cut(s) 221, 1390
RseI CAYNNNNRTG 2 cut(s) 705, 1040
SaqAI TTAA 5 cut(s) 116, 755, 1512, 1623, 2084
SatI GCNGC 8 cut(s) 747, 791, 1388, 1391, 1697, 1989, 2024, 2045
Sau3AI GATC 9 cut(s) 27, 150, 697, 802, 1014, 1168, 1528, 1579, 2011
Sau96I GGNCC 6 cut(s) 105, 350, 599, 1128, 1518, 2110
SchI GAGTC 4 cut(s) 218, 266, 581, 1919
ScrFI CCNGG 3 cut(s) 266, 425, 1001
SduI GDGCHC 5 cut(s) 325, 739, 1453, 1688, 1820
SfaNI GCATC 4 cut(s) 349, 697, 1545, 1864
SfcI CTRYAG 2 cut(s) 747, 1898
Sfr274I CTCGAG 1 cut(s) 875
SfuI TTCGAA 1 cut(s) 777
SinI GGWCC 3 cut(s) 350, 599, 1128
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 2 cut(s) 705, 1040
SmlI CTYRAG 3 cut(s) 875, 1472, 1907
SmoI CTYRAG 3 cut(s) 875, 1472, 1907
SphI GCATGC 1 cut(s) 1881
SsiI CCGC 2 cut(s) 348, 790
SspMI CTAG 8 cut(s) 399, 465, 612, 729, 896, 1322, 1394, 1490
StyD4I CCNGG 3 cut(s) 264, 423, 999
StyI CCWWGG 3 cut(s) 108, 398, 1230
TaaI ACNGT 4 cut(s) 238, 327, 988, 2094
TaqI TCGA 9 cut(s) 568, 777, 876, 891, 1333, 1403, 1422, 1453, 1835
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 3 cut(s) 250, 284, 725
Tru1I TTAA 5 cut(s) 116, 755, 1512, 1623, 2084
Tru9I TTAA 5 cut(s) 116, 755, 1512, 1623, 2084
TscAI CASTG 2 cut(s) 1928, 2003
TseFI GTSAC 1 cut(s) 1460
TseI GCWGC 7 cut(s) 746, 1387, 1390, 1696, 1988, 2023, 2044
Tsp45I GTSAC 1 cut(s) 1460
TspDTI ATGAA 9 cut(s) 664, 738, 892, 1052, 1107, 1179, 1279, 1509, 1681
TspGWI ACGGA 3 cut(s) 217, 953, 1034
TspRI CASTG 2 cut(s) 1928, 2003
VpaK11BI GGWCC 3 cut(s) 350, 599, 1128
XbaI TCTAGA 1 cut(s) 1489
XceI RCATGY 1 cut(s) 1881
XhoI CTCGAG 1 cut(s) 875
XmaJI CCTAGG 1 cut(s) 398
XmnI GAANNNNTTC 3 cut(s) 169, 671, 2094
XspI CTAG 8 cut(s) 399, 465, 612, 729, 896, 1322, 1394, 1490
Zsp2I ATGCAT 1 cut(s) 1879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.