RLG00000035880

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000618
Physical Location & Seq
Reverse (-)
73049964 .. 73050639
676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035880

Sequence Viewer

Length: 585 bp
ATGGACTACTTCAGTTGGAGCCTGGTTGAAGCCTTCGACAAGAGCATTCTTCTCTGGCACATTGCAACAGATTTATGCTTCTATAGTGATCTTGACAGATGCGATCACCAGGCTCCTAATTCCGAAATAGATTCCGAGACAAGTGCCGAGGCCACAACATTTTTTGAAAAGAGAAGAAATGTCATCGCCAAGGGTAGAAGCCATGCTCGTCAGGCACTTCTTGAGGTGAACACGGAGCTAAACCCAGCAGCAATTGTTAGAGATCAAAGCAAGACAGTGTTGTTCGAAGGTTGCATACTCGCGAAGCAGTTGAAGGCACTGGAAACAGAGCTGGAATGGAGTAACGAAGACAAATGGGAAATGATAAGTCATGTGTGGATAGAGATGTTGTGTTATGCAGCAAGCCAATGTGGATTGAAGGAGCACGATCAGCAGCTTAGGCGTGGGGGGGAGCTTATTGCTCATGTCTGCCTTCTCATGGCAAATCTTAGCTTGAGTGAAACAATTTCAGATAATACAAGGCTCAGTAAGAGTTCGTATAGAAGCTGTTCCACCCCGGAGCCCGCCAAACTAGCGTTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

195

Amino Acids

22.0

Weight (kDa)

5.15

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 113 - 162 2.5e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 302
AciI CCGC 1 cut(s) 564
AfiI CCNNNNNNNGG 1 cut(s) 478
AgsI TTSAA 4 cut(s) 29, 167, 313, 418
AjnI CCWGG 2 cut(s) 21, 108
AluBI AGCT 6 cut(s) 238, 331, 436, 454, 492, 546
AluI AGCT 6 cut(s) 238, 331, 436, 454, 492, 546
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 131
AoxI GGCC 2 cut(s) 150, 579
ApeKI GCWGC 3 cut(s) 248, 398, 433
Asp700I GAANNNNTTC 1 cut(s) 547
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 2 cut(s) 98, 238
AsuII TTCGAA 1 cut(s) 285
BanII GRGCYC 1 cut(s) 564
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 260, 410, 445
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 2 cut(s) 23, 110
BcnI CCSGG 1 cut(s) 557
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 3 cut(s) 249, 399, 434
BlsI GCNGC 3 cut(s) 250, 400, 435
Bme1390I CCNGG 3 cut(s) 23, 110, 557
BmiI GGNNCC 3 cut(s) 20, 114, 561
BmrFI CCNGG 3 cut(s) 23, 110, 557
BmsI GCATC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 242, 514
BpuMI CCSGG 1 cut(s) 557
BsaJI CCNNGG 3 cut(s) 147, 189, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 23, 110
BseDI CCNNGG 3 cut(s) 147, 189, 555
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 538
BseNI ACTGG 1 cut(s) 324
BseXI GCAGC 3 cut(s) 260, 410, 445
BseYI CCCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 2 cut(s) 152, 581
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 1 cut(s) 557
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 2 cut(s) 152, 581
Bsp119I TTCGAA 1 cut(s) 285
Bsp1286I GDGCHC 2 cut(s) 426, 564
Bsp143I GATC 4 cut(s) 88, 103, 262, 427
Bsp68I TCGCGA 1 cut(s) 302
BspACI CCGC 1 cut(s) 564
BspANI GGCC 2 cut(s) 152, 581
BspCNI CTCAG 1 cut(s) 537
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 3 cut(s) 20, 114, 561
BspT104I TTCGAA 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 324
BssECI CCNNGG 3 cut(s) 147, 189, 555
BssMI GATC 4 cut(s) 88, 103, 262, 427
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 2 cut(s) 23, 110
Bst4CI ACNGT 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 403, 564
BstDEI CTNAG 3 cut(s) 437, 488, 524
BstFNI CGCG 1 cut(s) 302
BstKTI GATC 4 cut(s) 91, 106, 265, 430
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 4 cut(s) 88, 103, 262, 427
BstMWI GCNNNNNNNGC 4 cut(s) 212, 430, 439, 572
BstNI CCWGG 2 cut(s) 23, 110
BstSCI CCNGG 3 cut(s) 21, 108, 555
BstSFI CTRYAG 1 cut(s) 82
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 3 cut(s) 260, 410, 445
BstV2I GAAGAC 1 cut(s) 354
BsuRI GGCC 2 cut(s) 152, 581
BtgZI GCGATG 1 cut(s) 169
BtsIMutI CAGTG 2 cut(s) 282, 317
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 403, 564
CviAII CATG 4 cut(s) 203, 371, 464, 478
DdeI CTNAG 3 cut(s) 437, 488, 524
DpnI GATC 4 cut(s) 90, 105, 264, 429
DpnII GATC 4 cut(s) 88, 103, 262, 427
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 21, 108
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 4 cut(s) 206, 374, 467, 481
FaiI YATR 9 cut(s) 76, 84, 204, 296, 372, 396, 465, 479, 540
FatI CATG 4 cut(s) 202, 370, 463, 477
FauI CCCGC 1 cut(s) 571
Fnu4HI GCNGC 3 cut(s) 249, 399, 434
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 3 cut(s) 249, 399, 434
FspBI CTAG 1 cut(s) 572
GluI GCNGC 3 cut(s) 249, 399, 434
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 2 cut(s) 152, 581
HapII CCGG 1 cut(s) 557
Hin1II CATG 4 cut(s) 206, 374, 467, 481
HinfI GANTC 1 cut(s) 131
HpaII CCGG 1 cut(s) 557
HphI GGTGA 2 cut(s) 98, 238
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 124, 136, 511
Hpy188III TCNNGA 3 cut(s) 92, 221, 301
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 5 cut(s) 43, 281, 307, 412, 482
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 3 cut(s) 65, 294, 398
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 430, 439, 572
HpyF3I CTNAG 3 cut(s) 437, 488, 524
Hsp92II CATG 4 cut(s) 206, 374, 467, 481
Kzo9I GATC 4 cut(s) 88, 103, 262, 427
LmnI GCTCC 6 cut(s) 18, 118, 235, 421, 451, 559
Lsp1109I GCAGC 3 cut(s) 260, 410, 445
LweI GCATC 1 cut(s) 89
MaeI CTAG 1 cut(s) 572
MaeIII GTNAC 1 cut(s) 341
MalI GATC 4 cut(s) 90, 105, 264, 429
MboI GATC 4 cut(s) 88, 103, 262, 427
MboII GAAGA 3 cut(s) 41, 186, 359
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 2 cut(s) 426, 564
MluCI AATT 3 cut(s) 118, 252, 504
MnlI CCTC 2 cut(s) 142, 217
MroXI GAANNNNTTC 1 cut(s) 547
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 3 cut(s) 23, 110, 557
MunI CAATTG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 45
MvaI CCWGG 2 cut(s) 23, 110
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 212, 430, 439, 572
NciI CCSGG 1 cut(s) 557
NdeII GATC 4 cut(s) 88, 103, 262, 427
NlaIII CATG 4 cut(s) 206, 374, 467, 481
NlaIV GGNNCC 3 cut(s) 20, 114, 561
NmeAIII GCCGAG 1 cut(s) 172
NruI TCGCGA 1 cut(s) 302
NspV TTCGAA 1 cut(s) 285
PctI GAATGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 3 cut(s) 250, 400, 435
Psp6I CCWGG 2 cut(s) 21, 108
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 2 cut(s) 21, 108
PspN4I GGNNCC 3 cut(s) 20, 114, 561
RruI TCGCGA 1 cut(s) 302
SatI GCNGC 3 cut(s) 249, 399, 434
Sau3AI GATC 4 cut(s) 88, 103, 262, 427
ScrFI CCNGG 3 cut(s) 23, 110, 557
SduI GDGCHC 2 cut(s) 426, 564
SetI ASST 8 cut(s) 228, 240, 292, 333, 438, 456, 494, 548
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 82
SfuI TTCGAA 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 221, 493
SmoI CTYRAG 2 cut(s) 221, 493
Sse9I AATT 3 cut(s) 118, 252, 504
SsiI CCGC 1 cut(s) 564
SspMI CTAG 1 cut(s) 572
StyD4I CCNGG 3 cut(s) 21, 108, 555
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 2 cut(s) 36, 285
TasI AATT 3 cut(s) 118, 252, 504
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 2 cut(s) 282, 324
TseI GCWGC 3 cut(s) 248, 398, 433
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 2 cut(s) 282, 324
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 547
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.