Prupe.3G040900_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000618
Physical Location & Seq
Forward (+)
2934606 .. 2937442
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G040900.1

Sequence Viewer

Length: 2058 bp
ATGATGCGGATTTTCCCAGAAAGTCTGAGCAAACTATGGAGCTTGTGGGAAATCGACGTAATGGTGGTATTAAGCCTCATCTTACAAGGCATTCTCATAGCTTTTGGCAACAAGAGGAAGTACTCAAACAGCAAATGGCTCCGGTTGTTGTGGCTTGCCTACTTGTCCGCAGACTGGGTTGCAACAGTATCCCTCAGCCTCCTTTCCAGCAATTTATCATCAACAAACCACAAAATGAATACCGCAGATGCAGACGACCAAGACGAGATCCTGACAGCATTTTGGGCCCCGTTTCTTCTGCTTCACCTTGGCGGCCCGGACACCATCACTGCATACTCTTTGGAAGACAATGAGTTGTGGTGGAGGCACTTCCTTGTGCTCCTCGTCCAGGTGTCCCTGGCTGTCTATGTGTTTCTTAGGGCCTGGGCTGGTCAGGCACTTAACCTTCTAACCATTCCAGTGTTTGTTGCTGGGATCATCAAGTTTGCGGAGCGGACTTGGGTTTTGAGGTGCGCAAGCAGTAAGCATTTCAGAGATTCTATGTTCTGTTGTCCTGACCCGGGGCCTGATTACGCCAGATACATGGACGAACTGCGCTCGAAGAAGCATGAAGGGTTCGAGGTTGACATAGAGAGAGTGGATGAGGCTCCCACCATTGGTGATTTTTCCTTTACAGCTCCAAGCATCTTTCCAAATACAGCCAATTTGCAGCATGCCGATGTCTTCTTCAACATTTTCAAAAAGCTATTTGCAGATCTCATCCTCAGCATCCATGATATCTTGAAAAGCAAATCCTTCTTCCAGAACAGATCCTACTATGAAGCTTTCAAAGTGATTGAGATTGAGCTTGGGTTCATGTATGATTTGTTTTATACAAAGGCTGTCCTGTATAGTCTCAACGGTGCCATTCTTCGTTTTACCAGTTCTGTTTCCATCATTTCCGTCTCTGTCGCCTTCCTCGTCACGATCGAGAAGCAAGATTATTCAGCAAGAAGTATAATTATTACTTATATGTTGCTGGCTGGTGCTATCATCCTCGATCTCTATGCTGTGATTTTATTTCTACGGTCAGATTGGGCATTGCTATGGTTTTGTAAGCACAAGACAGCAGCACATTTGTTGTATCCAGTGATTTCACATATGTCGTTCGTTGAAAACAAAAGGTGGTCTAATGAAATTACACAATACAACTTAATTTTGATCTGCTGCGAAGATAAGCCGGCCAAGTACAGGTTCCTCCAAAAGGTCCCCGGGATATGCAGAAAATTAAAGAAATCAGTTGAAGTCCCTAGAGAGTTGAAAGAACTGATATTTCTGCAGCTGTTAAAGAAATCAACATGTGCCCCAAACTCTGCGGGTTACAAGGAGTTAAGGGATCGTAGGGTCGAGTGGGTTCTTCAAAATGAGAATTGTATCGAAAAACTTGGCTGGAGCATTGGAGAAGAATTTGATCTCAGCGTTCTTCTTTGGCATATTGCAACGCAACTCTGTTATTACTCTGATCGTGATCAGGACAAATACCCAAACGCTTTCCCCGATCCTAATTGTGAAGCCAGCAAGTTGTTGTCCGAGTATATGTTGTATCTCCTAGTCAAGCGTTCCTCCATGCTTCCTAATGGGATCGGACAAATCAGGTTCAAGGACACATGCGCCGAAGCCACAGAATTCTTCAAACAAAGAAAATGCCAAAGATCAGAACAAGACCGGGCTTGCAAAAAACTACGCGAGGTGAACAGTGATGAGATCCTTCCAGCTGAAGTAAAAGGGGATGAAAGCAAGTCAGTGCTATTTGATGCTTGCAAGCTTGCTAAAGATTTGGAGTCGCTGGAAACAAAGGAAAATTGGGAGAATCAGAAGAAGTGGCAGTTGATCAGCCATGTGTGGGTGGAAATGCTATCTTATGCTGCCAGCCATTGTCGATGGAATCATCACGCTGTGCAACTCAGGCGCGGAGGAGAGCTGCTCACTCATGTCTGGCTTCTTATGGCACATCTTGGTTTAACTGAACAACTTCAAACTTTCGAACTTCAAAAGGCCATAGTATTGAATGTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

686

Amino Acids

79.29

Weight (kDa)

7.23

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 902
AccBSI CCGCTC 1 cut(s) 493
AccII CGCG 2 cut(s) 1725, 1950
AciI CCGC 9 cut(s) 7, 168, 243, 312, 488, 493, 1355, 1950, 2053
AclWI GGATC 7 cut(s) 262, 482, 804, 1383, 1532, 1628, 1738
AcoI YGGCCR 1 cut(s) 1221
AcsI RAATTY 2 cut(s) 1445, 1664
AcuI CTGAAG 1 cut(s) 1776
AdeI CACNNNGTG 1 cut(s) 1936
AfaI GTAC 2 cut(s) 122, 1229
AfiI CCNNNNNNNGG 7 cut(s) 174, 388, 560, 656, 1230, 1243, 1882
AflIII ACRYGT 1 cut(s) 1337
AjnI CCWGG 3 cut(s) 387, 396, 422
AjuI GAANNNNNNNTTGG 2 cut(s) 1217, 1249
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 899, 949
AlwI GGATC 7 cut(s) 262, 482, 804, 1383, 1532, 1628, 1738
Ama87I CYCGRG 2 cut(s) 559, 1250
AoxI GGCC 6 cut(s) 285, 313, 420, 563, 1221, 2034
ApaI GGGCCC 1 cut(s) 289
ApeKI GCWGC 6 cut(s) 709, 1109, 1206, 1318, 1904, 1960
ApoI RAATTY 2 cut(s) 1445, 1664
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
Asp700I GAANNNNTTC 1 cut(s) 2010
AspLEI GCGC 4 cut(s) 515, 597, 1652, 1950
AspS9I GGNCC 6 cut(s) 285, 286, 314, 420, 563, 1246
AsuC2I CCSGG 6 cut(s) 317, 560, 561, 1251, 1252, 1706
AsuHPI GGTGA 3 cut(s) 296, 671, 1741
AsuII TTCGAA 1 cut(s) 2022
AvaI CYCGRG 2 cut(s) 559, 1250
AvaII GGWCC 1 cut(s) 1246
BaeGI GKGCMC 2 cut(s) 289, 1345
BanI GGYRCC 1 cut(s) 902
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 2 cut(s) 351, 715
Bbv12I GWGCWC 1 cut(s) 381
BbvCI CCTCAGC 2 cut(s) 194, 764
BbvI GCAGC 6 cut(s) 721, 1121, 1193, 1330, 1891, 1947
BccI CCATC 3 cut(s) 332, 941, 1914
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 3 cut(s) 389, 398, 424
BciVI GTATCC 2 cut(s) 199, 1134
BclI TGATCA 2 cut(s) 1507, 1869
BcnI CCSGG 6 cut(s) 317, 560, 561, 1251, 1252, 1706
BcoDI GTCTC 2 cut(s) 899, 949
BfaI CTAG 2 cut(s) 1290, 1589
BfmI CTRYAG 1 cut(s) 1316
BfuI GTATCC 2 cut(s) 199, 1134
BglII AGATCT 1 cut(s) 754
BisI GCNGC 7 cut(s) 313, 710, 1110, 1207, 1319, 1905, 1961
BlsI GCNGC 7 cut(s) 314, 711, 1111, 1208, 1320, 1906, 1962
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 9 cut(s) 317, 389, 398, 424, 560, 561, 1251, 1252, 1706
Bme18I GGWCC 1 cut(s) 1246
BmeT110I CYCGRG 2 cut(s) 559, 1250
BmgT120I GGNCC 6 cut(s) 285, 286, 314, 420, 563, 1246
BmiI GGNNCC 8 cut(s) 140, 287, 288, 564, 648, 904, 1235, 1248
BmrFI CCNGG 9 cut(s) 317, 389, 398, 424, 560, 561, 1251, 1252, 1706
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 4 cut(s) 238, 693, 777, 1783
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 351, 715
BplI GAGNNNNNCTC 2 cut(s) 1947, 1979
BpmI CTGGAG 1 cut(s) 1450
Bpu10I CCTNAGC 2 cut(s) 194, 764
Bpu14I TTCGAA 1 cut(s) 2022
BpuMI CCSGG 6 cut(s) 317, 560, 561, 1251, 1252, 1706
BsaBI GATNNNNATC 1 cut(s) 1506
BsaJI CCNNGG 7 cut(s) 307, 396, 423, 559, 560, 1249, 1250
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 7 cut(s) 174, 388, 560, 656, 1230, 1243, 1882
Bse118I RCCGGY 1 cut(s) 1219
Bse1I ACTGG 4 cut(s) 179, 458, 921, 1127
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 1506
BseBI CCWGG 3 cut(s) 389, 398, 424
BseDI CCNNGG 7 cut(s) 307, 396, 423, 559, 560, 1249, 1250
BseGI GGATG 5 cut(s) 646, 759, 768, 1032, 1774
BseJI GATNNNNATC 1 cut(s) 1506
BseLI CCNNNNNNNGG 7 cut(s) 174, 388, 560, 656, 1230, 1243, 1882
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 5 cut(s) 17, 208, 778, 1468, 1957
BseNI ACTGG 4 cut(s) 179, 458, 921, 1127
BseRI GAGGAG 2 cut(s) 371, 1968
BseSI GKGCMC 2 cut(s) 289, 1345
BseXI GCAGC 6 cut(s) 721, 1121, 1193, 1330, 1891, 1947
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 2 cut(s) 1725, 1950
Bsh1285I CGRYCG 1 cut(s) 969
BshFI GGCC 6 cut(s) 287, 315, 422, 565, 1223, 2036
BshNI GGYRCC 1 cut(s) 902
BsiEI CGRYCG 1 cut(s) 969
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 559, 1250
BsiSI CCGG 6 cut(s) 142, 317, 560, 1220, 1251, 1705
BslFI GGGAC 3 cut(s) 379, 1232, 1271
BslI CCNNNNNNNGG 7 cut(s) 174, 388, 560, 656, 1230, 1243, 1882
BsmAI GTCTC 2 cut(s) 899, 949
BsmBI CGTCTC 1 cut(s) 949
BsmFI GGGAC 3 cut(s) 379, 1232, 1271
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 6 cut(s) 287, 315, 422, 565, 1223, 2036
BsoBI CYCGRG 2 cut(s) 559, 1250
Bsp119I TTCGAA 1 cut(s) 2022
Bsp120I GGGCCC 1 cut(s) 285
Bsp1286I GDGCHC 3 cut(s) 289, 381, 1345
BspACI CCGC 9 cut(s) 7, 168, 243, 312, 488, 493, 1355, 1950, 2053
BspANI GGCC 6 cut(s) 287, 315, 422, 565, 1223, 2036
BspCNI CTCAG 5 cut(s) 18, 207, 777, 1467, 1956
BspFNI CGCG 2 cut(s) 1725, 1950
BspLI GGNNCC 8 cut(s) 140, 287, 288, 564, 648, 904, 1235, 1248
BspMAI CTGCAG 1 cut(s) 1320
BspPI GGATC 7 cut(s) 262, 482, 804, 1383, 1532, 1628, 1738
BspT104I TTCGAA 1 cut(s) 2022
BspT107I GGYRCC 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 1219
BsrI ACTGG 4 cut(s) 179, 458, 921, 1127
BssAI RCCGGY 1 cut(s) 1219
BssECI CCNNGG 7 cut(s) 307, 396, 423, 559, 560, 1249, 1250
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 389, 398, 424
Bst4CI ACNGT 4 cut(s) 187, 902, 1068, 1736
BstBI TTCGAA 1 cut(s) 2022
BstDEI CTNAG 6 cut(s) 26, 194, 416, 764, 1454, 1943
BstENI CCTNNNNNAGG 2 cut(s) 386, 1241
BstF5I GGATG 5 cut(s) 646, 759, 768, 1032, 1774
BstFNI CGCG 2 cut(s) 1725, 1950
BstHHI GCGC 4 cut(s) 515, 597, 1652, 1950
BstMAI GTCTC 2 cut(s) 899, 949
BstMCI CGRYCG 1 cut(s) 969
BstMWI GCNNNNNNNGC 3 cut(s) 284, 434, 1945
BstNI CCWGG 3 cut(s) 389, 398, 424
BstNSI RCATGY 3 cut(s) 716, 1341, 1650
BstSCI CCNGG 9 cut(s) 315, 387, 396, 422, 558, 559, 1249, 1250, 1704
BstSFI CTRYAG 1 cut(s) 1316
BstSLI GKGCMC 2 cut(s) 289, 1345
BstUI CGCG 2 cut(s) 1725, 1950
BstV1I GCAGC 6 cut(s) 721, 1121, 1193, 1330, 1891, 1947
BstV2I GAAGAC 2 cut(s) 351, 715
BstX2I RGATCY 4 cut(s) 267, 754, 809, 1743
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 4 cut(s) 267, 754, 809, 1743
BsuI GTATCC 2 cut(s) 199, 1134
BsuRI GGCC 6 cut(s) 287, 315, 422, 565, 1223, 2036
BtsCI GGATG 5 cut(s) 646, 759, 768, 1032, 1774
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 5 cut(s) 327, 465, 1134, 1741, 1788
CfoI GCGC 4 cut(s) 515, 597, 1652, 1950
Cfr10I RCCGGY 1 cut(s) 1219
Cfr13I GGNCC 6 cut(s) 285, 286, 314, 420, 563, 1246
Cfr9I CCCGGG 2 cut(s) 559, 1250
Csp6I GTAC 2 cut(s) 121, 1228
CviQI GTAC 2 cut(s) 121, 1228
DdeI CTNAG 6 cut(s) 26, 194, 416, 764, 1454, 1943
DraIII CACNNNGTG 1 cut(s) 1936
EaeI YGGCCR 1 cut(s) 1221
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 1 cut(s) 1246
Eco57I CTGAAG 1 cut(s) 1776
Eco88I CYCGRG 2 cut(s) 559, 1250
EcoNI CCTNNNNNAGG 2 cut(s) 386, 1241
EcoO109I RGGNCCY 4 cut(s) 286, 420, 563, 1246
EcoRI GAATTC 1 cut(s) 1664
EcoRII CCWGG 3 cut(s) 387, 396, 422
EcoRV GATATC 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 307
Esp3I CGTCTC 1 cut(s) 949
FaqI GGGAC 3 cut(s) 379, 1232, 1271
FauI CCCGC 1 cut(s) 1348
FauNDI CATATG 1 cut(s) 1140
FbaI TGATCA 2 cut(s) 1507, 1869
Fnu4HI GCNGC 7 cut(s) 313, 710, 1110, 1207, 1319, 1905, 1961
FokI GGATG 5 cut(s) 653, 746, 755, 1019, 1781
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 7 cut(s) 313, 710, 1110, 1207, 1319, 1905, 1961
FspBI CTAG 2 cut(s) 1290, 1589
FspI TGCGCA 1 cut(s) 514
GlaI GCGC 4 cut(s) 514, 596, 1651, 1949
GluI GCNGC 7 cut(s) 313, 710, 1110, 1207, 1319, 1905, 1961
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 1450
HaeIII GGCC 6 cut(s) 287, 315, 422, 565, 1223, 2036
HapII CCGG 6 cut(s) 142, 317, 560, 1220, 1251, 1705
HhaI GCGC 4 cut(s) 515, 597, 1652, 1950
Hin6I GCGC 4 cut(s) 513, 595, 1650, 1948
HinP1I GCGC 4 cut(s) 513, 595, 1650, 1948
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 2 cut(s) 822, 1802
HinfI GANTC 4 cut(s) 536, 1820, 1849, 1924
HpaII CCGG 6 cut(s) 142, 317, 560, 1220, 1251, 1705
HphI GGTGA 3 cut(s) 296, 671, 1741
Hpy166II GTNNAC 2 cut(s) 625, 1732
Hpy188I TCNGA 8 cut(s) 27, 533, 1072, 1501, 1570, 1625, 1696, 1854
Hpy188III TCNNGA 8 cut(s) 271, 554, 781, 802, 964, 970, 1505, 1511
Hpy8I GTNNAC 2 cut(s) 625, 1732
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 5 cut(s) 455, 605, 805, 964, 1757
HpyCH4III ACNGT 4 cut(s) 187, 902, 1068, 1736
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 434, 1945
HpyF3I CTNAG 6 cut(s) 26, 194, 416, 764, 1454, 1943
HpySE526I ACGT 1 cut(s) 57
HspAI GCGC 4 cut(s) 513, 595, 1650, 1948
KroI GCCGGC 1 cut(s) 1219
KroNI GCCGGC 1 cut(s) 1221
Ksp22I TGATCA 2 cut(s) 1507, 1869
LmnI GCTCC 7 cut(s) 39, 144, 384, 490, 652, 682, 1431
Lsp1109I GCAGC 6 cut(s) 721, 1121, 1193, 1330, 1891, 1947
LweI GCATC 4 cut(s) 238, 693, 777, 1783
MaeI CTAG 2 cut(s) 1290, 1589
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 961, 1358
MbiI CCGCTC 1 cut(s) 493
MflI RGATCY 4 cut(s) 267, 754, 809, 1743
MhlI GDGCHC 3 cut(s) 289, 381, 1345
MlyI GAGTC 1 cut(s) 1829
MroNI GCCGGC 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 2010
MseI TTAA 7 cut(s) 71, 441, 1193, 1268, 1325, 1370, 2000
MslI CAYNNNNRTG 3 cut(s) 458, 717, 1084
MspA1I CMGCKG 2 cut(s) 1321, 1754
MspI CCGG 6 cut(s) 142, 317, 560, 1220, 1251, 1705
MspR9I CCNGG 9 cut(s) 317, 389, 398, 424, 560, 561, 1251, 1252, 1706
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 3 cut(s) 389, 398, 424
MvnI CGCG 2 cut(s) 1725, 1950
MwoI GCNNNNNNNGC 3 cut(s) 284, 434, 1945
NaeI GCCGGC 1 cut(s) 1221
NciI CCSGG 6 cut(s) 317, 560, 561, 1251, 1252, 1706
NdeI CATATG 1 cut(s) 1140
NgoMIV GCCGGC 1 cut(s) 1219
NlaIV GGNNCC 8 cut(s) 140, 287, 288, 564, 648, 904, 1235, 1248
NmuCI GTSAC 1 cut(s) 961
NsbI TGCGCA 1 cut(s) 514
NspI RCATGY 3 cut(s) 716, 1341, 1650
NspV TTCGAA 1 cut(s) 2022
PaeI GCATGC 1 cut(s) 716
PciI ACATGT 1 cut(s) 1337
PcsI WCGNNNNNNNCGW 4 cut(s) 261, 957, 966, 1533
PctI GAATGC 1 cut(s) 90
PdiI GCCGGC 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 2010
PfeI GAWTC 3 cut(s) 536, 1849, 1924
PkrI GCNGC 7 cut(s) 314, 711, 1111, 1208, 1320, 1906, 1962
Ple19I CGATCG 1 cut(s) 969
PleI GAGTC 1 cut(s) 1828
PpsI GAGTC 1 cut(s) 1828
PpuMI RGGWCCY 1 cut(s) 1246
PscI ACATGT 1 cut(s) 1337
Psp5II RGGWCCY 1 cut(s) 1246
Psp6I CCWGG 3 cut(s) 387, 396, 422
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 3 cut(s) 387, 396, 422
PspN4I GGNNCC 8 cut(s) 140, 287, 288, 564, 648, 904, 1235, 1248
PspOMI GGGCCC 1 cut(s) 285
PspPI GGNCC 6 cut(s) 285, 286, 314, 420, 563, 1246
PspPPI RGGWCCY 1 cut(s) 1246
PsrI GAACNNNNNNTAC 2 cut(s) 797, 829
PstI CTGCAG 1 cut(s) 1320
PsuI RGATCY 4 cut(s) 267, 754, 809, 1743
PvuI CGATCG 1 cut(s) 969
PvuII CAGCTG 2 cut(s) 1321, 1754
RsaI GTAC 2 cut(s) 122, 1229
RsaNI GTAC 2 cut(s) 121, 1228
RseI CAYNNNNRTG 3 cut(s) 458, 717, 1084
SaqAI TTAA 7 cut(s) 71, 441, 1193, 1268, 1325, 1370, 2000
SatI GCNGC 7 cut(s) 313, 710, 1110, 1207, 1319, 1905, 1961
Sau96I GGNCC 6 cut(s) 285, 286, 314, 420, 563, 1246
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 1 cut(s) 1829
ScrFI CCNGG 9 cut(s) 317, 389, 398, 424, 560, 561, 1251, 1252, 1706
SduI GDGCHC 3 cut(s) 289, 381, 1345
SfaNI GCATC 4 cut(s) 238, 693, 777, 1783
SfcI CTRYAG 1 cut(s) 1316
SfuI TTCGAA 1 cut(s) 2022
SinI GGWCC 1 cut(s) 1246
SmaI CCCGGG 2 cut(s) 561, 1252
SmiMI CAYNNNNRTG 3 cut(s) 458, 717, 1084
SphI GCATGC 1 cut(s) 716
SsiI CCGC 9 cut(s) 7, 168, 243, 312, 488, 493, 1355, 1950, 2053
SspMI CTAG 2 cut(s) 1290, 1589
StyD4I CCNGG 9 cut(s) 315, 387, 396, 422, 558, 559, 1249, 1250, 1704
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 4 cut(s) 187, 902, 1068, 1736
TaiI ACGT 1 cut(s) 60
TaqI TCGA 9 cut(s) 54, 599, 618, 969, 1038, 1386, 1416, 1918, 2022
TatI WGTACW 2 cut(s) 120, 1227
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 3 cut(s) 536, 1849, 1924
Tru1I TTAA 7 cut(s) 71, 441, 1193, 1268, 1325, 1370, 2000
Tru9I TTAA 7 cut(s) 71, 441, 1193, 1268, 1325, 1370, 2000
TscAI CASTG 5 cut(s) 334, 465, 1134, 1741, 1788
TseFI GTSAC 1 cut(s) 961
TseI GCWGC 6 cut(s) 709, 1109, 1206, 1318, 1904, 1960
Tsp45I GTSAC 1 cut(s) 961
TspDTI ATGAA 6 cut(s) 251, 624, 834, 844, 1188, 1785
TspGWI ACGGA 1 cut(s) 931
TspMI CCCGGG 2 cut(s) 559, 1250
TspRI CASTG 5 cut(s) 334, 465, 1134, 1741, 1788
VpaK11BI GGWCC 1 cut(s) 1246
XagI CCTNNNNNAGG 2 cut(s) 386, 1241
XapI RAATTY 2 cut(s) 1445, 1664
XceI RCATGY 3 cut(s) 716, 1341, 1650
XmaI CCCGGG 2 cut(s) 559, 1250
XmnI GAANNNNTTC 1 cut(s) 2010
XspI CTAG 2 cut(s) 1290, 1589
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.