MD11G1092500.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000618
Physical Location & Seq
Forward (+)
7673893 .. 7675980
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1092500.v1.1.491

Sequence Viewer

Length: 2088 bp
ATGGTGGAAATTAGAATTATTCCAGAAAGTGTGGGAAACCTATGGAACAAATGGAACCTCCGACTCCTGGTCCTCTGCAGCCTATGCTTACAAACTGTCCTAGTGGTGTTGGGAAGCCGAAGAAAGTACAGCGCTAAAACTTGGATCAGAATCGTCATTTGGACAGCGTACTTATCAGCGGACTGGGTTGCTACTGTCACGCTTGGAATGATATCCAGCTTCCAAGTAGACGATCAAGAAAGCTCCCCACATCCAAACAACCATACCATTATGGCTTTCTGGGCGCCATTTCTTCTCGTGCACCTTGGCGGTCCAGACACCATCACTGCTTACGCGTTGGAGGACAATGAGCTTTGGTTGAGGCACCTGCTGGGTTTAGTTGTCCAGGTTGAAGTGGCTTTTTTCATCTTTTTAAGATACTGGACGCCGAGCACGTACCCCCTCTCATTGCTAGCAATACCAGTATTTGTTGTTGGACTTACCAAGTATGGAGAGCGGACTTGGGCCCTGCGGTCTGCAAGCAGTGGAACATTGCGTGACTCCTTGCTCCCTGCTCCGAATCCAGGTCGTGGTTATGCCAATTATGCAGCTGAATATAGCGCTATCAAAGCTCGAGGAGATGAGGTTGCAATTGGAAGTACAGGTGGTGAAGGTATTTTTCGTGTTGGGCGGATCAATCCTGTTATTTCCGAAGCCAGATATCTCCACGAAGCATTTGAATTGTTCGACCTCTTTAAGCGCCTTTACGCAGATCTCATCCTGGACTACAATGATCGCAATTGCTGCAGTGAAGTGCTTCAGGGTCATTCACCGAAAGAAGTCCTCAAAGTAATAGAGGTTGAGCTTGGTTTTGTTTATGATGTGTTATACACAAAGGCAACTGTTGTGTATTCTCGCCCGGGTATTATTTTTTGCTTCATCAACCTTCTTTGCTCCATTTCCACACTTGTAGTCTTTGCCTCTGTTGTTGATAAGCATTTTTATTCCACCGTTGATATAACTCTCACCTACTTATTGTCTGCCGGAGCCATTGCTCTTGAGTTGTACGCCATCATTGTACTAATTTGTACAGATTGGACACTCCATTGGTTAAGCAAGCGGACGCACTCACTTGCTTCTCGTGTCTATCAAGCAATCTCTTCCTTTAGATCAAGCTGGGGCTACGACAAAAATTGGTCTGAATCCGTGGCACAATACAATCTCATCGATTTTTGTTTCAAAGAGAATCCCCCGAAGTGTGCTAGAATTCTCAAACTCCTTCACATCTATGACATGTTAGAGAAATATTGGTACACAGCCAAGGAGGATGTCCCTACTGAGATGAGAGAGCTTATTGTTGCGCAACTCCAAGATGCTCAAAGAGAAAATGTTGCAACCAGTGAAGTCTTGGATTGGAGAGGCACTCATGCTCTTCAAAAGAGTAACTGTATCGAAAAGTTTGGTTGGAGTGTGGAAGAAGCTTCATTAGACCAAAGCATTCTTCTATGGCACATTGCAACAGATTTATGCTTCAACAATGATCTTGAAAGTATAAATCAAGATCCATCTCTAATTAGTTCCAAATCCAAGGTGAGCAAGTTGTTCTCAAATTATATGATGCACATTCTCGTCTTCAGCCCCTTCATGTTGCCAGAGGGAATTGGGCAGATTAGGTTTCGTGACACGTGCACAGAGGCCACAGAGTTTTTTGAAAAGAGAAGAGAAGTCATAACCAAAGGGGCAGGCCATGCCAGGCAGGTTTTGCTTGAGGTGAACACGGAGTTAGTTCCGGCAATGGTGATTGGTGATAGAAGCAAGACAGTGTTGTTTGATGGGTGTAGGCTTGCAAAACAGTTGATGGCATTGGAAACCGAGGAGGGAAAGAGTAATGAAGAAAAATGGGGGATAATAAGCCAAGTGTGGATAGAGATGTTGTGTTACGCAGCAAGTCAATGCAGTTGGAAGGAGCACGCGCAACAGCTTAGGCATGGAGGAGAGCTTCTTACACATGTGTGCCTTCTCATGGCAAATCTTGGTTTCAGCCAACAGTTTCAGATCAAACAAGGCACACCAACAGTAACTCTTTCTGCTATTAGCAAGCAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

696

Amino Acids

78.8

Weight (kDa)

6.06

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 54 - 404 7.5e-100 Domain of unknown function (DUF4220)
DUF594 PF04578 621 - 672 5.3e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 1341
Acc36I ACCTGC 2 cut(s) 375, 1726
AccB1I GGYRCC 2 cut(s) 283, 363
AccB7I CCANNNNNTGG 1 cut(s) 569
AccBSI CCGCTC 1 cut(s) 496
AccI GTMKAC 1 cut(s) 228
AccII CGCG 2 cut(s) 335, 1952
AciI CCGC 6 cut(s) 179, 309, 496, 511, 670, 1099
AclWI GGATC 3 cut(s) 152, 680, 1535
AcsI RAATTY 1 cut(s) 1245
AcuI CTGAAG 2 cut(s) 782, 1597
AcvI CACGTG 1 cut(s) 1665
AcyI GRCGYC 2 cut(s) 284, 425
AfaI GTAC 8 cut(s) 128, 170, 437, 640, 1046, 1059, 1069, 1292
AfeI AGCGCT 2 cut(s) 133, 601
AfiI CCNNNNNNNGG 4 cut(s) 67, 563, 569, 2002
AflIII ACRYGT 4 cut(s) 333, 1272, 1662, 1987
AgsI TTSAA 7 cut(s) 392, 719, 1219, 1415, 1513, 1526, 1691
AhdI GACNNNNNGTC 1 cut(s) 68
AjnI CCWGG 5 cut(s) 66, 384, 562, 759, 1730
AjuI GAANNNNNNNTTGG 4 cut(s) 142, 174, 1464, 1496
AleI CACNNNNGTG 1 cut(s) 1990
Alw21I GWGCWC 4 cut(s) 303, 434, 1670, 1950
Alw44I GTGCAC 2 cut(s) 299, 1666
AlwI GGATC 3 cut(s) 152, 680, 1535
Ama87I CYCGRG 2 cut(s) 612, 898
Aor51HI AGCGCT 2 cut(s) 133, 601
AoxI GGCC 3 cut(s) 504, 1674, 1723
ApaI GGGCCC 1 cut(s) 508
ApaLI GTGCAC 2 cut(s) 299, 1666
ApeKI GCWGC 4 cut(s) 78, 587, 783, 1922
ApoI RAATTY 1 cut(s) 1245
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 6 cut(s) 134, 286, 602, 741, 1342, 1954
AspS9I GGNCC 4 cut(s) 70, 311, 504, 505
AsuC2I CCSGG 2 cut(s) 899, 900
AsuHPI GGTGA 7 cut(s) 659, 801, 997, 1582, 1762, 1789, 1796
AsuNHI GCTAGC 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 612, 898
AvaII GGWCC 2 cut(s) 70, 311
BaeGI GKGCMC 3 cut(s) 303, 508, 1670
BanI GGYRCC 2 cut(s) 283, 363
BanII GRGCYC 1 cut(s) 508
BauI CACGAG 2 cut(s) 296, 1119
BbrPI CACGTG 1 cut(s) 1665
BbsI GAAGAC 1 cut(s) 1603
Bbv12I GWGCWC 4 cut(s) 303, 434, 1670, 1950
BbvI GCAGC 4 cut(s) 90, 599, 770, 1934
BccI CCATC 5 cut(s) 329, 1058, 1552, 1805, 1831
BciT130I CCWGG 5 cut(s) 68, 386, 564, 761, 1732
BcnI CCSGG 2 cut(s) 899, 900
BfaI CTAG 4 cut(s) 101, 452, 1242, 2086
BfmI CTRYAG 2 cut(s) 76, 784
BfoI RGCGCY 4 cut(s) 135, 287, 603, 742
BfuAI ACCTGC 2 cut(s) 375, 1726
BglII AGATCT 1 cut(s) 751
BisI GCNGC 4 cut(s) 79, 588, 784, 1923
BlsI GCNGC 4 cut(s) 80, 589, 785, 1924
Bme1390I CCNGG 7 cut(s) 68, 386, 564, 761, 899, 900, 1732
Bme18I GGWCC 2 cut(s) 70, 311
BmeRI GACNNNNNGTC 1 cut(s) 68
BmeT110I CYCGRG 2 cut(s) 612, 898
BmgT120I GGNCC 4 cut(s) 70, 311, 504, 505
BmiI GGNNCC 5 cut(s) 56, 285, 365, 506, 1027
BmrFI CCNGG 7 cut(s) 68, 386, 564, 761, 899, 900, 1732
BmrI ACTGGG 1 cut(s) 193
BmsI GCATC 2 cut(s) 1342, 1587
BmtI GCTAGC 1 cut(s) 455
BmuI ACTGGG 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 1603
BplI GAGNNNNNCTC 2 cut(s) 1387, 1419
Bpu10I CCTNAGC 1 cut(s) 1961
BpuEI CTTGAG 2 cut(s) 1058, 1766
BpuMI CCSGG 2 cut(s) 899, 900
Bsa29I ATCGAT 1 cut(s) 1206
BsaAI YACGTR 2 cut(s) 435, 1665
BsaBI GATNNNNATC 1 cut(s) 149
BsaHI GRCGYC 2 cut(s) 284, 425
BsaJI CCNNGG 6 cut(s) 304, 898, 1185, 1299, 1566, 1851
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 563, 569, 2002
Bse1I ACTGG 4 cut(s) 188, 425, 461, 1377
Bse3DI GCAATG 5 cut(s) 446, 530, 1029, 1491, 1779
Bse8I GATNNNNATC 1 cut(s) 149
BseBI CCWGG 5 cut(s) 68, 386, 564, 761, 1732
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 6 cut(s) 304, 898, 1185, 1299, 1566, 1851
BseGI GGATG 3 cut(s) 250, 756, 1312
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 4 cut(s) 67, 563, 569, 2002
BseMI GCAATG 5 cut(s) 446, 530, 1029, 1491, 1779
BseMII CTCAG 1 cut(s) 1308
BseNI ACTGG 4 cut(s) 188, 425, 461, 1377
BseRI GAGGAG 3 cut(s) 630, 1868, 1986
BseSI GKGCMC 3 cut(s) 303, 508, 1670
BseXI GCAGC 4 cut(s) 90, 599, 770, 1934
BseYI CCCAGC 2 cut(s) 370, 1155
Bsh1236I CGCG 2 cut(s) 335, 1952
BshFI GGCC 3 cut(s) 506, 1676, 1725
BshNI GGYRCC 2 cut(s) 283, 363
BshVI ATCGAT 1 cut(s) 1206
BsiHKAI GWGCWC 4 cut(s) 303, 434, 1670, 1950
BsiHKCI CYCGRG 2 cut(s) 612, 898
BsiSI CCGG 3 cut(s) 899, 1023, 1769
BslFI GGGAC 1 cut(s) 1295
BslI CCNNNNNNNGG 4 cut(s) 67, 563, 569, 2002
BsmFI GGGAC 1 cut(s) 1295
BsmI GAATGC 1 cut(s) 1476
BsnI GGCC 3 cut(s) 506, 1676, 1725
BsoBI CYCGRG 2 cut(s) 612, 898
Bsp120I GGGCCC 1 cut(s) 504
Bsp1286I GDGCHC 5 cut(s) 303, 434, 508, 1670, 1950
Bsp1407I TGTACA 1 cut(s) 1067
Bsp143I GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
BspACI CCGC 6 cut(s) 179, 309, 496, 511, 670, 1099
BspANI GGCC 3 cut(s) 506, 1676, 1725
BspCNI CTCAG 1 cut(s) 1309
BspDI ATCGAT 1 cut(s) 1206
BspFNI CGCG 2 cut(s) 335, 1952
BspLI GGNNCC 5 cut(s) 56, 285, 365, 506, 1027
BspMAI CTGCAG 2 cut(s) 80, 788
BspMI ACCTGC 2 cut(s) 375, 1726
BspOI GCTAGC 1 cut(s) 455
BspPI GGATC 3 cut(s) 152, 680, 1535
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 2 cut(s) 283, 363
BsrBI CCGCTC 1 cut(s) 496
BsrDI GCAATG 5 cut(s) 446, 530, 1029, 1491, 1779
BsrGI TGTACA 1 cut(s) 1067
BsrI ACTGG 4 cut(s) 188, 425, 461, 1377
BssECI CCNNGG 6 cut(s) 304, 898, 1185, 1299, 1566, 1851
BssMI GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
BssNI GRCGYC 2 cut(s) 284, 425
BssSI CACGAG 2 cut(s) 296, 1119
BssT1I CCWWGG 3 cut(s) 304, 1299, 1566
Bst2BI CACGAG 2 cut(s) 296, 1119
Bst2UI CCWGG 5 cut(s) 68, 386, 564, 761, 1732
Bst4CI ACNGT 9 cut(s) 97, 196, 883, 991, 1427, 1801, 1833, 2028, 2056
Bst6I CTCTTC 3 cut(s) 1144, 1416, 1693
BstACI GRCGYC 2 cut(s) 284, 425
BstAPI GCANNNNNTGC 4 cut(s) 84, 783, 1727, 1741
BstAUI TGTACA 1 cut(s) 1067
BstBAI YACGTR 2 cut(s) 435, 1665
BstC8I GCNNGC 7 cut(s) 453, 520, 1097, 1723, 1824, 1950, 2078
BstDEI CTNAG 2 cut(s) 1317, 1961
BstDSI CCRYGG 1 cut(s) 1185
BstF5I GGATG 3 cut(s) 250, 756, 1312
BstFNI CGCG 2 cut(s) 335, 1952
BstH2I RGCGCY 4 cut(s) 135, 287, 603, 742
BstHHI GCGC 6 cut(s) 134, 286, 602, 741, 1342, 1954
BstKTI GATC 9 cut(s) 147, 235, 675, 754, 775, 1151, 1522, 1543, 2037
BstMBI GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
BstMWI GCNNNNNNNGC 7 cut(s) 84, 281, 584, 608, 783, 1727, 1741
BstNI CCWGG 5 cut(s) 68, 386, 564, 761, 1732
BstNSI RCATGY 2 cut(s) 1276, 1991
BstSCI CCNGG 7 cut(s) 66, 384, 562, 759, 897, 898, 1730
BstSFI CTRYAG 2 cut(s) 76, 784
BstSLI GKGCMC 3 cut(s) 303, 508, 1670
BstUI CGCG 2 cut(s) 335, 1952
BstV1I GCAGC 4 cut(s) 90, 599, 770, 1934
BstV2I GAAGAC 1 cut(s) 1603
BstX2I RGATCY 2 cut(s) 751, 1540
BstYI RGATCY 2 cut(s) 751, 1540
Bsu15I ATCGAT 1 cut(s) 1206
BsuRI GGCC 3 cut(s) 506, 1676, 1725
BsuTUI ATCGAT 1 cut(s) 1206
BtgI CCRYGG 1 cut(s) 1185
BtsCI GGATG 3 cut(s) 250, 756, 1312
BtsI GCAGTG 3 cut(s) 324, 529, 793
BtsIMutI CAGTG 5 cut(s) 324, 529, 793, 1384, 1806
BveI ACCTGC 2 cut(s) 375, 1726
Cac8I GCNNGC 7 cut(s) 453, 520, 1097, 1723, 1824, 1950, 2078
CfoI GCGC 6 cut(s) 134, 286, 602, 741, 1342, 1954
Cfr13I GGNCC 4 cut(s) 70, 311, 504, 505
Cfr9I CCCGGG 1 cut(s) 898
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 2 cut(s) 433, 1111
Csp6I GTAC 8 cut(s) 127, 169, 436, 639, 1045, 1058, 1068, 1291
CviAII CATG 7 cut(s) 1273, 1406, 1624, 1727, 1967, 1988, 2002
CviQI GTAC 8 cut(s) 127, 169, 436, 639, 1045, 1058, 1068, 1291
DdeI CTNAG 2 cut(s) 1317, 1961
DinI GGCGCC 1 cut(s) 285
DpnI GATC 9 cut(s) 146, 234, 674, 753, 774, 1150, 1521, 1542, 2036
DpnII GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
DriI GACNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 3 cut(s) 1144, 1416, 1693
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 3 cut(s) 1144, 1416, 1693
EciI GGCGGA 1 cut(s) 685
Eco130I CCWWGG 3 cut(s) 304, 1299, 1566
Eco24I GRGCYC 1 cut(s) 508
Eco32I GATATC 2 cut(s) 213, 701
Eco47I GGWCC 2 cut(s) 70, 311
Eco47III AGCGCT 2 cut(s) 133, 601
Eco57I CTGAAG 2 cut(s) 782, 1597
Eco72I CACGTG 1 cut(s) 1665
Eco88I CYCGRG 2 cut(s) 612, 898
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 5 cut(s) 66, 384, 562, 759, 1730
EcoRV GATATC 2 cut(s) 213, 701
EcoT14I CCWWGG 3 cut(s) 304, 1299, 1566
EcoT38I GRGCYC 1 cut(s) 508
EgeI GGCGCC 1 cut(s) 285
EheI GGCGCC 1 cut(s) 285
ErhI CCWWGG 3 cut(s) 304, 1299, 1566
FaeI CATG 7 cut(s) 1276, 1409, 1627, 1730, 1970, 1991, 2005
FaqI GGGAC 1 cut(s) 1295
FatI CATG 7 cut(s) 1272, 1405, 1623, 1726, 1966, 1987, 2001
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 79, 588, 784, 1923
FokI GGATG 3 cut(s) 237, 743, 1319
FriOI GRGCYC 1 cut(s) 508
Fsp4HI GCNGC 4 cut(s) 79, 588, 784, 1923
FspBI CTAG 4 cut(s) 101, 452, 1242, 2086
FspI TGCGCA 1 cut(s) 1341
GlaI GCGC 6 cut(s) 133, 285, 601, 740, 1341, 1953
GluI GCNGC 4 cut(s) 79, 588, 784, 1923
GsaI CCCAGC 2 cut(s) 374, 1159
HaeII RGCGCY 4 cut(s) 135, 287, 603, 742
HaeIII GGCC 3 cut(s) 506, 1676, 1725
HapII CCGG 3 cut(s) 899, 1023, 1769
HgaI GACGC 2 cut(s) 433, 1111
HhaI GCGC 6 cut(s) 134, 286, 602, 741, 1342, 1954
Hin1I GRCGYC 2 cut(s) 284, 425
Hin1II CATG 7 cut(s) 1276, 1409, 1627, 1730, 1970, 1991, 2005
Hin6I GCGC 6 cut(s) 132, 284, 600, 739, 1340, 1952
HinP1I GCGC 6 cut(s) 132, 284, 600, 739, 1340, 1952
HindIII AAGCTT 1 cut(s) 1458
HinfI GANTC 6 cut(s) 63, 150, 539, 559, 1181, 1225
HpaII CCGG 3 cut(s) 899, 1023, 1769
HphI GGTGA 7 cut(s) 659, 801, 997, 1582, 1762, 1789, 1796
Hpy166II GTNNAC 5 cut(s) 229, 301, 1293, 1668, 1753
Hpy188I TCNGA 6 cut(s) 62, 149, 558, 691, 1180, 2034
Hpy188III TCNNGA 7 cut(s) 23, 236, 314, 1037, 1523, 1538, 1658
Hpy8I GTNNAC 5 cut(s) 229, 301, 1293, 1668, 1753
HpyAV CCTTC 6 cut(s) 644, 935, 1268, 1630, 1936, 2006
HpyCH4III ACNGT 9 cut(s) 97, 196, 883, 991, 1427, 1801, 1833, 2028, 2056
HpyCH4IV ACGT 2 cut(s) 434, 1664
HpyF10VI GCNNNNNNNGC 7 cut(s) 84, 281, 584, 608, 783, 1727, 1741
HpyF3I CTNAG 2 cut(s) 1317, 1961
HpySE526I ACGT 2 cut(s) 434, 1664
Hsp92I GRCGYC 2 cut(s) 284, 425
Hsp92II CATG 7 cut(s) 1276, 1409, 1627, 1730, 1970, 1991, 2005
HspAI GCGC 6 cut(s) 132, 284, 600, 739, 1340, 1952
KasI GGCGCC 1 cut(s) 283
Kzo9I GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 6 cut(s) 248, 552, 559, 938, 1025, 1945
Lsp1109I GCAGC 4 cut(s) 90, 599, 770, 1934
LweI GCATC 2 cut(s) 1342, 1587
MaeI CTAG 4 cut(s) 101, 452, 1242, 2086
MaeII ACGT 2 cut(s) 434, 1664
MaeIII GTNAC 6 cut(s) 196, 536, 1421, 1658, 1916, 2056
MalI GATC 9 cut(s) 146, 234, 674, 753, 774, 1150, 1521, 1542, 2036
MbiI CCGCTC 1 cut(s) 496
MboI GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
MboII GAAGA 9 cut(s) 132, 284, 1131, 1403, 1466, 1472, 1603, 1710, 1883
MfeI CAATTG 2 cut(s) 630, 778
MflI RGATCY 2 cut(s) 751, 1540
MhlI GDGCHC 5 cut(s) 303, 434, 508, 1670, 1950
MluI ACGCGT 1 cut(s) 333
Mly113I GGCGCC 1 cut(s) 284
MlyI GAGTC 2 cut(s) 57, 533
MmeI TCCRAC 5 cut(s) 85, 318, 454, 1424, 1919
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 3 cut(s) 413, 735, 1091
MslI CAYNNNNRTG 2 cut(s) 1266, 1990
MspA1I CMGCKG 2 cut(s) 179, 590
MspI CCGG 3 cut(s) 899, 1023, 1769
MspR9I CCNGG 7 cut(s) 68, 386, 564, 761, 899, 900, 1732
MunI CAATTG 2 cut(s) 630, 778
Mva1269I GAATGC 1 cut(s) 1476
MvaI CCWGG 5 cut(s) 68, 386, 564, 761, 1732
MvnI CGCG 2 cut(s) 335, 1952
MwoI GCNNNNNNNGC 7 cut(s) 84, 281, 584, 608, 783, 1727, 1741
NarI GGCGCC 1 cut(s) 284
NciI CCSGG 2 cut(s) 899, 900
NdeII GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
NheI GCTAGC 1 cut(s) 451
NlaIII CATG 7 cut(s) 1276, 1409, 1627, 1730, 1970, 1991, 2005
NlaIV GGNNCC 5 cut(s) 56, 285, 365, 506, 1027
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 3 cut(s) 196, 536, 1658
NsbI TGCGCA 1 cut(s) 1341
NspI RCATGY 2 cut(s) 1276, 1991
OliI CACNNNNGTG 1 cut(s) 1990
PaeR7I CTCGAG 1 cut(s) 612
PaqCI CACCTGC 1 cut(s) 375
PciI ACATGT 2 cut(s) 1272, 1987
PciSI GCTCTTC 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PctI GAATGC 1 cut(s) 1476
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 4 cut(s) 150, 559, 1181, 1225
PflMI CCANNNNNTGG 1 cut(s) 569
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 4 cut(s) 80, 589, 785, 1924
PleI GAGTC 2 cut(s) 57, 533
PluTI GGCGCC 1 cut(s) 287
PmaCI CACGTG 1 cut(s) 1665
PmlI CACGTG 1 cut(s) 1665
PpsI GAGTC 2 cut(s) 57, 533
Ppu21I YACGTR 2 cut(s) 435, 1665
PscI ACATGT 2 cut(s) 1272, 1987
Psp6I CCWGG 5 cut(s) 66, 384, 562, 759, 1730
PspCI CACGTG 1 cut(s) 1665
PspFI CCCAGC 2 cut(s) 370, 1155
PspGI CCWGG 5 cut(s) 66, 384, 562, 759, 1730
PspN4I GGNNCC 5 cut(s) 56, 285, 365, 506, 1027
PspOMI GGGCCC 1 cut(s) 504
PspPI GGNCC 4 cut(s) 70, 311, 504, 505
PspXI VCTCGAGB 1 cut(s) 612
PstI CTGCAG 2 cut(s) 80, 788
PsuI RGATCY 2 cut(s) 751, 1540
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 8 cut(s) 128, 170, 437, 640, 1046, 1059, 1069, 1292
RsaNI GTAC 8 cut(s) 127, 169, 436, 639, 1045, 1058, 1068, 1291
RseI CAYNNNNRTG 2 cut(s) 1266, 1990
SapI GCTCTTC 1 cut(s) 1416
SaqAI TTAA 3 cut(s) 413, 735, 1091
SatI GCNGC 4 cut(s) 79, 588, 784, 1923
Sau3AI GATC 9 cut(s) 144, 232, 672, 751, 772, 1148, 1519, 1540, 2034
Sau96I GGNCC 4 cut(s) 70, 311, 504, 505
SchI GAGTC 2 cut(s) 57, 533
ScrFI CCNGG 7 cut(s) 68, 386, 564, 761, 899, 900, 1732
SduI GDGCHC 5 cut(s) 303, 434, 508, 1670, 1950
SfaNI GCATC 2 cut(s) 1342, 1587
SfcI CTRYAG 2 cut(s) 76, 784
SfoI GGCGCC 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 612
SinI GGWCC 2 cut(s) 70, 311
SlaI CTCGAG 1 cut(s) 612
SmaI CCCGGG 1 cut(s) 900
SmiMI CAYNNNNRTG 2 cut(s) 1266, 1990
SmlI CTYRAG 3 cut(s) 612, 1037, 1745
SmoI CTYRAG 3 cut(s) 612, 1037, 1745
SsiI CCGC 6 cut(s) 179, 309, 496, 511, 670, 1099
SspDI GGCGCC 1 cut(s) 283
SspI AATATT 1 cut(s) 1286
SspMI CTAG 4 cut(s) 101, 452, 1242, 2086
StyD4I CCNGG 7 cut(s) 66, 384, 562, 759, 897, 898, 1730
StyI CCWWGG 3 cut(s) 304, 1299, 1566
TaaI ACNGT 9 cut(s) 97, 196, 883, 991, 1427, 1801, 1833, 2028, 2056
TaiI ACGT 2 cut(s) 437, 1667
TaqI TCGA 4 cut(s) 613, 726, 1206, 1431
TatI WGTACW 4 cut(s) 126, 638, 1057, 1067
TfiI GAWTC 4 cut(s) 150, 559, 1181, 1225
Tru1I TTAA 3 cut(s) 413, 735, 1091
Tru9I TTAA 3 cut(s) 413, 735, 1091
TscAI CASTG 5 cut(s) 331, 529, 793, 1384, 1806
TseFI GTSAC 3 cut(s) 196, 536, 1658
TseI GCWGC 4 cut(s) 78, 587, 783, 1922
Tsp45I GTSAC 3 cut(s) 196, 536, 1658
TspDTI ATGAA 5 cut(s) 394, 907, 1452, 1612, 1884
TspGWI ACGGA 2 cut(s) 1174, 1772
TspMI CCCGGG 1 cut(s) 898
TspRI CASTG 5 cut(s) 331, 529, 793, 1384, 1806
Van91I CCANNNNNTGG 1 cut(s) 569
VneI GTGCAC 2 cut(s) 299, 1666
VpaK11BI GGWCC 2 cut(s) 70, 311
XapI RAATTY 1 cut(s) 1245
XceI RCATGY 2 cut(s) 1276, 1991
XcmI CCANNNNNNNNNTGG 1 cut(s) 1384
XhoI CTCGAG 1 cut(s) 612
XmaI CCCGGG 1 cut(s) 898
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 4 cut(s) 101, 452, 1242, 2086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.