Prupe.4G109300_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000618
Physical Location & Seq
Forward (+)
5725446 .. 5728900
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G109300.1

Sequence Viewer

Length: 2070 bp
ATGACCACAATGCAACTTATCCCTGAAAGTTTGAGAAAACTTTGGAACGAGTGGGAGCTTCGGGGTATGGTTCTAGCCAGTCTTGCCTTGCAAATTATCCTCATCCTGATTGGCAACTGGAGAAAACATTCAACCAGTAACAAGCTCAGAATTCTTCTGTGGCTCTCTTACCTGTCAGCTGACTCAGTTGCAACAGTTGCACTTGGCATACTCTCCAGCAACCAAGAAGAATCTCAAGGTGATTCCGTAAACCCGAAAGTTGTAATAACCGCATTTTGGGCACCCTTTCTTCTCTTACATCTTGGTGGCCCAGATACTATCACTGCTTACTCATTAGAAGACAATGAGTTGTGGCTTAGGCACTTGCTTGGGCTATTTGTCCAGGTGGTTGTAGCTTTATATGTCGCTTTCAGAGCATGGTCCAGTAAAGAGTTAAATTTTTTGGCAATACCAATGTTCATAGTTGGGATGATCAAGTTTGGGGAGAGGACATGGGTTCTAAGATCGGCAAGCAGTGAGCATTTTAGAGATTCTATGCTTCATGATCCTGACCCAGGTCCCAATTATGCTAGATACATGGAAGAATATTGTTCAAAGAGAGCCCAGGGCTTCAGAGTTAACTCGGTTGAACTAGGAGGGGATAACTCACTAGTTGATGCAGTTGTTGACGGTACCCAAAATGTGGCAATTTTAGACAAGGCCTACGACTTCTTTGAAACATTCAAACTGCTTTGTGCTGATCTCATCCTCAGCTTCCATGATATAGTACACAGCCACTCCTTCTTAGAAAATACAAACTCCGATCAAGCTTTCGAAGTTATTGAGTTTGAGCTAGGATTCATGTATGATGCCTTTTATACTAAGTCAGTTCTGGTTTCTTCTGGTCTGGGTTGCATTCTCCGCGGTATTACTGTTTCTATAACACTTTCTGTATTCATAGCCTTCCTGTTCTCAAAGAAGCAAGCATACAGTGGAGTAGATGTAATTATCACATATATATTGTTGATAGGAGCAATAGTCCTAGAATCTTATGCTGTAGCATTGCTGCTTTCCTCAGACAGGACAAGGCTATGGCTGAATAAGCAAAAAAATATGGTGGCACGTCTCATGGTTCTCTTGCGCAGTGCCGTTTCGTCAGTCCCTTTGGGTTATAACAAGAGGTGGTCTAATACCTTGGCACAATACAACCTCATCACATTTTGTCTTAAGGCTAAGCCAACGAAATGCATTTCTGTCCAGAAGTTCTTATTCATTTATCAGTTGTTAGAGAAGTATCGATACAAAGAGTTGGTGGATATCCCCACTGAGTTGAAGGAGTTGATATTTGAGCAGCTCCAAGAGAAATCAAGGATTGGTTCCAACGTTGAGGCTAGCAAACGAAAACAAGTATGTGCTCGAAGGGGTGACCAGGTGCTTCAAAAAGCACATTGTCTAAAGGAACTTGGCTGGACCATCAATGAGGTTGAGTTTGATCAAAGCATCCTTCTCTGGCATATTGCAACAGATCTATGTTATTATTCCGATGTGAATAGAAACTCGAACTCGGTTCCTAGTGAAAATTGTGAAGACAGTAAGCTGTTATCGAACTATATGATGTATCTTTTAGTCATGTGCCCCTTCATGCTACCAAATGGGATTGGACAGATAAGGTTTAGAGACACATGTGCTGAGGCTGAAGAATTTTTCAAGGAAAAGAAAATAATGAAATCGGGTGAAAGGAAAGCATGCACAAAGCTATTGGATGTGTGCACAGACATCCTTCCATCTAAAGTGAAAGGAGATAGAAGCAAGTCAGTTCTATTTGATGCATGCAGGCTTGCTAAAGCTTTGCAGTCCCTGGAATCAGATAGAGGGTGGAGTAATGAGAGAAAGTGGGAGTTTGTAAGTCATGTGTGGGTGGAAATGCTCTCCTACGCTGCCAATCAGTGTCGGTGGAGTGGTCATGCTCAGCAGCTCAGGCGAGGAGGAGAGCTGCTCACCCATGTCTGGCTTCTGATGGCACATCTGGGTTTAACTGAACAGTTCCAAATTTCAGAGGGCCATGCTAGGGCAAAACTGATTGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

690

Amino Acids

78.55

Weight (kDa)

7.53

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1152
Acc16I TGCGCA 1 cut(s) 1121
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 2 cut(s) 280, 671
AccB7I CCANNNNNTGG 1 cut(s) 682
AccII CGCG 1 cut(s) 903
AciI CCGC 3 cut(s) 270, 901, 903
AclI AACGTT 1 cut(s) 1362
AclWI GGATC 1 cut(s) 539
AcsI RAATTY 4 cut(s) 150, 436, 1679, 2028
AcuI CTGAAG 2 cut(s) 595, 1695
AfaI GTAC 2 cut(s) 673, 768
AfiI CCNNNNNNNGG 6 cut(s) 276, 554, 682, 1059, 1986, 2047
AflII CTTAAG 1 cut(s) 1205
AflIII ACRYGT 1 cut(s) 1661
AgsI TTSAA 8 cut(s) 132, 594, 629, 716, 724, 1312, 1418, 1687
AhlI ACTAGT 1 cut(s) 649
AjiI CACGTC 1 cut(s) 1103
AjnI CCWGG 5 cut(s) 381, 553, 603, 1407, 1836
Alw21I GWGCWC 2 cut(s) 1396, 1751
Alw26I GTCTC 2 cut(s) 1109, 1650
Alw44I GTGCAC 1 cut(s) 1747
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 1837
AoxI GGCC 3 cut(s) 307, 699, 2038
ApaLI GTGCAC 1 cut(s) 1747
ApeKI GCWGC 5 cut(s) 1045, 1330, 1916, 1951, 1972
ApoI RAATTY 4 cut(s) 150, 436, 1679, 2028
Asp700I GAANNNNTTC 1 cut(s) 127
Asp718I GGTACC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1122
AspS9I GGNCC 5 cut(s) 308, 420, 557, 1449, 2038
AsuHPI GGTGA 4 cut(s) 251, 1415, 1724, 1969
AsuII TTCGAA 1 cut(s) 813
AsuNHI GCTAGC 1 cut(s) 1370
AvaII GGWCC 3 cut(s) 420, 557, 1449
BaeGI GKGCMC 3 cut(s) 283, 1616, 1751
BanI GGYRCC 2 cut(s) 280, 671
BanII GRGCYC 1 cut(s) 604
BarI GAAGNNNNNNTAC 4 cut(s) 950, 982, 1262, 1294
BbsI GAAGAC 2 cut(s) 345, 1572
Bbv12I GWGCWC 2 cut(s) 1396, 1751
BbvCI CCTCAGC 2 cut(s) 749, 1668
BbvI GCAGC 5 cut(s) 1032, 1342, 1903, 1959, 1963
BccI CCATC 3 cut(s) 1460, 1771, 1990
BceAI ACGGC 1 cut(s) 1112
BciT130I CCWGG 5 cut(s) 383, 555, 605, 1409, 1838
BclI TGATCA 2 cut(s) 471, 1471
BcoDI GTCTC 2 cut(s) 1109, 1650
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 9 cut(s) 74, 570, 632, 650, 833, 1022, 1371, 1551, 2046
BfmI CTRYAG 1 cut(s) 1035
BfrI CTTAAG 1 cut(s) 1205
BglII AGATCT 1 cut(s) 1504
BisI GCNGC 5 cut(s) 1046, 1331, 1917, 1952, 1973
BlpI GCTNAGC 2 cut(s) 1212, 1947
BlsI GCNGC 5 cut(s) 1047, 1332, 1918, 1953, 1974
Bme1390I CCNGG 5 cut(s) 383, 555, 605, 1409, 1838
Bme18I GGWCC 3 cut(s) 420, 557, 1449
BmgBI CACGTC 1 cut(s) 1103
BmgT120I GGNCC 5 cut(s) 308, 420, 557, 1449, 2038
BmiI GGNNCC 5 cut(s) 282, 559, 673, 1357, 1548
BmrFI CCNGG 5 cut(s) 383, 555, 605, 1409, 1838
BmsI GCATC 4 cut(s) 646, 838, 1488, 1795
BmtI GCTAGC 1 cut(s) 1374
BoxI GACNNNNGTC 1 cut(s) 555
BpiI GAAGAC 2 cut(s) 345, 1572
BplI GAGNNNNNCTC 2 cut(s) 1959, 1991
BpmI CTGGAG 2 cut(s) 139, 199
Bpu10I CCTNAGC 4 cut(s) 356, 749, 1668, 1955
Bpu1102I GCTNAGC 2 cut(s) 1212, 1947
Bpu14I TTCGAA 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 1276
BsaJI CCNNGG 6 cut(s) 553, 603, 604, 901, 1173, 1836
BsaXI ACNNNNNCTCC 6 cut(s) 761, 791, 966, 996, 1002, 1032
Bsc4I CCNNNNNNNGG 6 cut(s) 276, 554, 682, 1059, 1986, 2047
Bse1I ACTGG 4 cut(s) 78, 122, 135, 423
Bse3DI GCAATG 1 cut(s) 1040
BseBI CCWGG 5 cut(s) 383, 555, 605, 1409, 1838
BseCI ATCGAT 1 cut(s) 1276
BseDI CCNNGG 6 cut(s) 553, 603, 604, 901, 1173, 1836
BseGI GGATG 6 cut(s) 102, 474, 744, 1479, 1747, 1755
BseLI CCNNNNNNNGG 6 cut(s) 276, 554, 682, 1059, 1986, 2047
BseMI GCAATG 1 cut(s) 1040
BseMII CTCAG 8 cut(s) 160, 198, 763, 1068, 1296, 1659, 1961, 1969
BseNI ACTGG 4 cut(s) 78, 122, 135, 423
BseRI GAGGAG 2 cut(s) 1977, 1980
BseSI GKGCMC 3 cut(s) 283, 1616, 1751
BseXI GCAGC 5 cut(s) 1032, 1342, 1903, 1959, 1963
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 3 cut(s) 309, 701, 2040
BshNI GGYRCC 2 cut(s) 280, 671
BshVI ATCGAT 1 cut(s) 1276
BsiHKAI GWGCWC 2 cut(s) 1396, 1751
BslFI GGGAC 3 cut(s) 543, 1124, 1819
BslI CCNNNNNNNGG 6 cut(s) 276, 554, 682, 1059, 1986, 2047
BsmAI GTCTC 2 cut(s) 1109, 1650
BsmBI CGTCTC 1 cut(s) 1109
BsmFI GGGAC 3 cut(s) 543, 1124, 1819
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 3 cut(s) 309, 701, 2040
Bsp119I TTCGAA 1 cut(s) 813
Bsp1286I GDGCHC 5 cut(s) 283, 604, 1396, 1616, 1751
Bsp143I GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
Bsp1720I GCTNAGC 2 cut(s) 1212, 1947
BspACI CCGC 3 cut(s) 270, 901, 903
BspANI GGCC 3 cut(s) 309, 701, 2040
BspCNI CTCAG 8 cut(s) 159, 197, 762, 1067, 1297, 1660, 1960, 1968
BspDI ATCGAT 1 cut(s) 1276
BspFNI CGCG 1 cut(s) 903
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 5 cut(s) 282, 559, 673, 1357, 1548
BspOI GCTAGC 1 cut(s) 1374
BspPI GGATC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 813
BspT107I GGYRCC 2 cut(s) 280, 671
BspTI CTTAAG 1 cut(s) 1205
BsrDI GCAATG 1 cut(s) 1040
BsrI ACTGG 4 cut(s) 78, 122, 135, 423
BssECI CCNNGG 6 cut(s) 553, 603, 604, 901, 1173, 1836
BssMI GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 5 cut(s) 383, 555, 605, 1409, 1838
Bst4CI ACNGT 6 cut(s) 196, 671, 913, 971, 1571, 2022
BstAFI CTTAAG 1 cut(s) 1205
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 7 cut(s) 511, 963, 1372, 1726, 1810, 1814, 1818
BstDSI CCRYGG 1 cut(s) 901
BstEII GGTNACC 1 cut(s) 1403
BstENI CCTNNNNNAGG 2 cut(s) 552, 1057
BstF5I GGATG 6 cut(s) 102, 474, 744, 1479, 1747, 1755
BstFNI CGCG 1 cut(s) 903
BstHHI GCGC 1 cut(s) 1122
BstKTI GATC 7 cut(s) 474, 506, 547, 742, 805, 1474, 1507
BstMAI GTCTC 2 cut(s) 1109, 1650
BstMBI GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
BstMWI GCNNNNNNNGC 7 cut(s) 83, 197, 278, 413, 900, 1081, 1957
BstNI CCWGG 5 cut(s) 383, 555, 605, 1409, 1838
BstNSI RCATGY 3 cut(s) 1665, 1728, 1812
BstPAI GACNNNNGTC 1 cut(s) 555
BstPI GGTNACC 1 cut(s) 1403
BstSCI CCNGG 5 cut(s) 381, 553, 603, 1407, 1836
BstSFI CTRYAG 1 cut(s) 1035
BstSLI GKGCMC 3 cut(s) 283, 1616, 1751
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 5 cut(s) 1032, 1342, 1903, 1959, 1963
BstV2I GAAGAC 2 cut(s) 345, 1572
BstX2I RGATCY 1 cut(s) 1504
BstYI RGATCY 1 cut(s) 1504
Bsu15I ATCGAT 1 cut(s) 1276
BsuRI GGCC 3 cut(s) 309, 701, 2040
BsuTUI ATCGAT 1 cut(s) 1276
BtgI CCRYGG 1 cut(s) 901
BtrI CACGTC 1 cut(s) 1103
BtsCI GGATG 6 cut(s) 102, 474, 744, 1479, 1747, 1755
BtsI GCAGTG 3 cut(s) 321, 520, 1129
BtsIMutI CAGTG 6 cut(s) 321, 520, 976, 1129, 1302, 1931
Cac8I GCNNGC 7 cut(s) 511, 963, 1372, 1726, 1810, 1814, 1818
CaiI CAGNNNCTG 1 cut(s) 1837
CciI TCATGA 1 cut(s) 541
CfoI GCGC 1 cut(s) 1122
Cfr13I GGNCC 5 cut(s) 308, 420, 557, 1449, 2038
Cfr42I CCGCGG 1 cut(s) 904
ClaI ATCGAT 1 cut(s) 1276
CsiI ACCWGGT 1 cut(s) 1407
Csp6I GTAC 2 cut(s) 672, 767
CviQI GTAC 2 cut(s) 672, 767
DpnI GATC 7 cut(s) 473, 505, 546, 741, 804, 1473, 1506
DpnII GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
Eco130I CCWWGG 1 cut(s) 1173
Eco147I AGGCCT 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 604
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 3 cut(s) 420, 557, 1449
Eco57I CTGAAG 2 cut(s) 595, 1695
Eco91I GGTNACC 1 cut(s) 1403
EcoNI CCTNNNNNAGG 2 cut(s) 552, 1057
EcoO109I RGGNCCY 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 1403
EcoRI GAATTC 1 cut(s) 150
EcoRII CCWGG 5 cut(s) 381, 553, 603, 1407, 1836
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 2 cut(s) 1229, 1810
EcoT38I GRGCYC 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 1173
Esp3I CGTCTC 1 cut(s) 1109
FaqI GGGAC 3 cut(s) 543, 1124, 1819
FbaI TGATCA 2 cut(s) 471, 1471
Fnu4HI GCNGC 5 cut(s) 1046, 1331, 1917, 1952, 1973
FokI GGATG 6 cut(s) 89, 481, 731, 1466, 1742, 1754
FriOI GRGCYC 1 cut(s) 604
Fsp4HI GCNGC 5 cut(s) 1046, 1331, 1917, 1952, 1973
FspBI CTAG 9 cut(s) 74, 570, 632, 650, 833, 1022, 1371, 1551, 2046
FspI TGCGCA 1 cut(s) 1121
GlaI GCGC 1 cut(s) 1121
GluI GCNGC 5 cut(s) 1046, 1331, 1917, 1952, 1973
GsuI CTGGAG 2 cut(s) 139, 199
HaeIII GGCC 3 cut(s) 309, 701, 2040
HhaI GCGC 1 cut(s) 1122
Hin6I GCGC 1 cut(s) 1120
HinP1I GCGC 1 cut(s) 1120
HincII GTYRAC 2 cut(s) 619, 667
HindII GTYRAC 2 cut(s) 619, 667
HindIII AAGCTT 2 cut(s) 807, 1824
HinfI GANTC 7 cut(s) 182, 230, 242, 530, 837, 1025, 1841
HpaI GTTAAC 1 cut(s) 619
HphI GGTGA 4 cut(s) 251, 1415, 1724, 1969
Hpy166II GTNNAC 5 cut(s) 250, 619, 667, 769, 1749
Hpy188I TCNGA 9 cut(s) 149, 413, 614, 802, 1057, 1522, 1846, 1995, 2035
Hpy188III TCNNGA 4 cut(s) 106, 542, 548, 1237
Hpy8I GTNNAC 5 cut(s) 250, 619, 667, 769, 1749
HpyAV CCTTC 7 cut(s) 790, 952, 1306, 1392, 1493, 1627, 1769
HpyCH4III ACNGT 6 cut(s) 196, 671, 913, 971, 1571, 2022
HpyCH4IV ACGT 2 cut(s) 1102, 1362
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 197, 278, 413, 900, 1081, 1957
HpySE526I ACGT 2 cut(s) 1102, 1362
HspAI GCGC 1 cut(s) 1120
KpnI GGTACC 1 cut(s) 675
Ksp22I TGATCA 2 cut(s) 471, 1471
KspAI GTTAAC 1 cut(s) 619
KspI CCGCGG 1 cut(s) 904
Kzo9I GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
LmnI GCTCC 3 cut(s) 55, 1010, 1338
Lsp1109I GCAGC 5 cut(s) 1032, 1342, 1903, 1959, 1963
LweI GCATC 4 cut(s) 646, 838, 1488, 1795
MabI ACCWGGT 1 cut(s) 1407
MaeI CTAG 9 cut(s) 74, 570, 632, 650, 833, 1022, 1371, 1551, 2046
MaeII ACGT 2 cut(s) 1102, 1362
MaeIII GTNAC 2 cut(s) 137, 1403
MalI GATC 7 cut(s) 473, 505, 546, 741, 804, 1473, 1506
MboI GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
MboII GAAGA 8 cut(s) 146, 239, 281, 350, 593, 870, 1577, 1688
MflI RGATCY 1 cut(s) 1504
MhlI GDGCHC 5 cut(s) 283, 604, 1396, 1616, 1751
MluCI AATT 9 cut(s) 93, 150, 436, 562, 687, 984, 1558, 1679, 2028
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 1383
Mph1103I ATGCAT 2 cut(s) 1229, 1810
MroXI GAANNNNTTC 1 cut(s) 127
MseI TTAA 4 cut(s) 434, 618, 1206, 2012
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 2 cut(s) 179, 903
MspCI CTTAAG 1 cut(s) 1205
MspR9I CCNGG 5 cut(s) 383, 555, 605, 1409, 1838
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 5 cut(s) 383, 555, 605, 1409, 1838
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 7 cut(s) 83, 197, 278, 413, 900, 1081, 1957
NdeII GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
NheI GCTAGC 1 cut(s) 1370
NlaIV GGNNCC 5 cut(s) 282, 559, 673, 1357, 1548
NmuCI GTSAC 1 cut(s) 1403
NsbI TGCGCA 1 cut(s) 1121
NsiI ATGCAT 2 cut(s) 1229, 1810
NspI RCATGY 3 cut(s) 1665, 1728, 1812
NspV TTCGAA 1 cut(s) 813
PaeI GCATGC 2 cut(s) 1728, 1812
PagI TCATGA 1 cut(s) 541
PasI CCCWGGG 1 cut(s) 604
PceI AGGCCT 1 cut(s) 701
PciI ACATGT 1 cut(s) 1661
PctI GAATGC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 6 cut(s) 230, 242, 530, 837, 1025, 1841
PflMI CCANNNNNTGG 1 cut(s) 682
PkrI GCNGC 5 cut(s) 1047, 1332, 1918, 1953, 1974
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 557
PscI ACATGT 1 cut(s) 1661
PshAI GACNNNNGTC 1 cut(s) 555
PsiI TTATAA 1 cut(s) 1152
Psp1406I AACGTT 1 cut(s) 1362
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 5 cut(s) 381, 553, 603, 1407, 1836
PspEI GGTNACC 1 cut(s) 1403
PspGI CCWGG 5 cut(s) 381, 553, 603, 1407, 1836
PspN4I GGNNCC 5 cut(s) 282, 559, 673, 1357, 1548
PspPI GGNCC 5 cut(s) 308, 420, 557, 1449, 2038
PspPPI RGGWCCY 1 cut(s) 557
PstNI CAGNNNCTG 1 cut(s) 1837
PsuI RGATCY 1 cut(s) 1504
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 2 cut(s) 673, 768
RsaNI GTAC 2 cut(s) 672, 767
RseI CAYNNNNRTG 1 cut(s) 303
SacII CCGCGG 1 cut(s) 904
SaqAI TTAA 4 cut(s) 434, 618, 1206, 2012
SatI GCNGC 5 cut(s) 1046, 1331, 1917, 1952, 1973
Sau3AI GATC 7 cut(s) 471, 503, 544, 739, 802, 1471, 1504
Sau96I GGNCC 5 cut(s) 308, 420, 557, 1449, 2038
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 5 cut(s) 383, 555, 605, 1409, 1838
SduI GDGCHC 5 cut(s) 283, 604, 1396, 1616, 1751
SexAI ACCWGGT 1 cut(s) 1407
SfaNI GCATC 4 cut(s) 646, 838, 1488, 1795
SfcI CTRYAG 1 cut(s) 1035
Sfr303I CCGCGG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 813
SgrBI CCGCGG 1 cut(s) 904
SinI GGWCC 3 cut(s) 420, 557, 1449
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 2 cut(s) 234, 1205
SmoI CTYRAG 2 cut(s) 234, 1205
SpeI ACTAGT 1 cut(s) 649
SphI GCATGC 2 cut(s) 1728, 1812
Sse9I AATT 9 cut(s) 93, 150, 436, 562, 687, 984, 1558, 1679, 2028
SseBI AGGCCT 1 cut(s) 701
SsiI CCGC 3 cut(s) 270, 901, 903
SspI AATATT 1 cut(s) 587
SspMI CTAG 9 cut(s) 74, 570, 632, 650, 833, 1022, 1371, 1551, 2046
StuI AGGCCT 1 cut(s) 701
StyD4I CCNGG 5 cut(s) 381, 553, 603, 1407, 1836
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 6 cut(s) 196, 671, 913, 971, 1571, 2022
TaiI ACGT 2 cut(s) 1105, 1365
TaqI TCGA 5 cut(s) 813, 1276, 1396, 1538, 1583
TasI AATT 9 cut(s) 93, 150, 436, 562, 687, 984, 1558, 1679, 2028
TatI WGTACW 1 cut(s) 766
TfiI GAWTC 6 cut(s) 230, 242, 530, 837, 1025, 1841
Tru1I TTAA 4 cut(s) 434, 618, 1206, 2012
Tru9I TTAA 4 cut(s) 434, 618, 1206, 2012
TscAI CASTG 6 cut(s) 328, 520, 976, 1129, 1309, 1931
TseFI GTSAC 1 cut(s) 1403
TseI GCWGC 5 cut(s) 1045, 1330, 1916, 1951, 1972
Tsp45I GTSAC 1 cut(s) 1403
TspDTI ATGAA 7 cut(s) 448, 530, 829, 925, 1240, 1609, 1718
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 6 cut(s) 328, 520, 976, 1129, 1309, 1931
Van91I CCANNNNNTGG 1 cut(s) 682
Vha464I CTTAAG 1 cut(s) 1205
VneI GTGCAC 1 cut(s) 1747
VpaK11BI GGWCC 3 cut(s) 420, 557, 1449
XagI CCTNNNNNAGG 2 cut(s) 552, 1057
XapI RAATTY 4 cut(s) 150, 436, 1679, 2028
XceI RCATGY 3 cut(s) 1665, 1728, 1812
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 9 cut(s) 74, 570, 632, 650, 833, 1022, 1371, 1551, 2046
Zsp2I ATGCAT 2 cut(s) 1229, 1810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.