Prupe.3G041600_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000618
Physical Location & Seq
Reverse (-)
2968175 .. 2971155
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G041600.1

Sequence Viewer

Length: 2118 bp
ATGCTACTCATAGTTGTCAGCAGTTTTATCTTCACAGAGAAGAGGATGGCGATGCAGATTTTCCCGGAAAGTGTGAGAAAAATCTGGAACGAGTGGGAGCTTCGGGCTATGGTTTTGATCAGCCTCAGCTTGCAGGCCATCCTCATCCTCATTGGCAGCTGGAGAAAGCACTCAACCAGTAACATACTCAGAATTGTTCTGTGGCTTGCTTACTTGTCAGCAGACTCAGTTGCAACAGTCTCACTTGGCATACTCTCCAACAACCAAGAAGACTCTCCAGGTGATTCTGTCAATCCAGATTATATAATAACAGCATTTTGGGCACCCTTTCTTCTCTTGCATCTTGGTGGCCCAGATACAATTACTGCTTACTCCTTAGAAGACAATGAATTGTGGTTGAGGCACTTGCTTGGGGTAGCTGTCCAGGTGCTTGTAGCTTTCTATGTCTTCTTAAGAGCATGGTCCAATAAAGTGCTGAATTTTTTGGCAATACCAATGTTCATAGTTGGGATTATAAAGTTTGGGGAGAGGACTTGGGTTTTAAGGTCTGCAAGTAGTGAGCATTTTAGAGAGTCTATGCTTCCTCATCCTGATCCTGGTCCCAGTTATGCTAGATACATGGAAGAATACAGCTCTAAGAAAGCTGAGGGATTCAAGGTTGAACTAGGACCACCCATTGAAGCTCCCAAAGCTGAGAATAATTCCAACGATGCCGGAGGAGTACTTGATGACAGTATCCAAAATGCTGTAATTTTAAACAAAGCCTACAGTTTCTTTGAAACTTTCAAGCGGCTATGTGCTGATCTCATCCTCAGCTTCCACAATATAGAGAAAAGCCAAACATTCTTCCAAAATAGAAACTCCGAGCAAGCTTTCGAAGTCATTGAGATTGAGCTAGGATTCATGTATGATGTGTTTTATACTAGAGCTGTCCTAGTTCATTCTCGTCTCGGTGGTATTCTCCGTTGTATCAGCCTTTCTCTCACAGTTTCAGTATTCCTGGTCTTCTTGTTCAAAGAGAAGCATGCATACACGGGAGAGGATGTAGCTATCACATATGTCTTGTTGGTAGGAGCTATCATCCTGGAAATGTATGCTGTGGTCATGCTACTTTCCTCAGACTGGACAACGCTACAGCTGAATAAGCACAAAAATGGGGTGTTGAAGCTCTTCCATACAGCCGTTTCATCAATCCCATTGGTTAAAAGAAATAGGTGGTCTAATAAATTGGGACAATACAACCTAATAACATTTTGTCTCAAAAATAGGCCAGCCAAGTGCATTTTTATTAAGAAGGACTTATTCATAAATAAGTTGTTAGAGAAGTATCGATACCAAGACTCGGAGGATGTTTCCAAAGAGTTGAAGAATTTGATATTTACGCAGCTCAAAGAGAAATCAAAGAGTGCTTCTAATTTTGATGCTTGCAAGCAATTATGTGCTCGTAGGGGTGCCCAGGTGCTTCGAGATGCAAAGTGTCTAGTTAGACTTGGCTGGACCATTTTTGGGGTTGAATTTGAACAAAGCATCCTTCTCTGGCATATTGCAACAGATCTTTGTTATAATTTTGATGTGAAGAGAAACTCGAACGTTGTACCTAACCAAAATTGTAAAAACAGCAAGCAGTTGTCGGACTACATGTTGTACCTTTTAGTCATGTGCCCCTTCATGCTGCCAAATGGGATTGGGCAGATAAGGTTTCAAGACACGTGTGCTGAGGCTGAGGACTTTTTCACAGAAAGAAAATCCATAACCGACGAGGAAAAAGCTTGTACAATGCTACTCAAAGTGAGCACAGACATCTCTCCATCTGAAGTGAAGGGAGATCGAAGCAAGTCGGTTCTATTTGATGCATGCAGGCTTGCTAAAGCTTTGCAGTCCATGGAGGTTGAGGGACATTGGGGGACTAATGAGAAAAAGTGGGAGTTGGTAAGTCAAGTGTGGGTGGAAATGCTCTCTTACGCAGCCAATCGGTGTCGCTGGAGTGATCATGCTCAACAGCTCAGGAGAGGAGGCGAGCTGCTCACTCATGTTTGGCTGCTTATGGCACATCTTGGTTTGACTGAACAGTTCCAAATTTCAGAGGGCCATAAAAGGGCAAAGCTGGTTGTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

706

Amino Acids

80.59

Weight (kDa)

8.36

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04580 FvH4_3g37050 FvH4_3g37060 FvH4_4g30270 FvH4_6g34780 FvH4_6g34780
malus_domestica MD11G1092500.v1.1 MD17G1025500.v1.1
prunus_persica Prupe.1G206300_v2.0.a1 Prupe.3G040700_v2.0.a1 Prupe.3G040900_v2.0.a1 Prupe.3G041300_v2.0.a1 Prupe.3G041600_v2.0.a1 Prupe.3G061600_v2.0.a1 Prupe.4G109300_v2.0.a1 Prupe.6G069600_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.6G069800_v2.0.a1 Prupe.8G014800_v2.0.a1 Prupe.8G037600_v2.0.a1
pyrus_communis pycom09g10050 pycom17g16040
rosa_chinensis RchiOBHm_Chr2g0145601 RchiOBHm_Chr5g0066461 RchiOBHm_Chr5g0066471 RchiOBHm_Chr5g0066481
rosa_laevigata RLG00000019691 RLG00000020148 RLG00000020153 RLG00000035869 RLG00000035878 RLG00000035880
rosa_multiflora Rmu_co8287763.1_g000001 Rmu_co8480723.1_g000001 Rmu_co8493537.1_g000001 Rmu_sc0002170.1_g000044 Rmu_sc0002599.1_g000001 Rmu_sc0002718.1_g000013 Rmu_sc0003046.1_g000008 Rmu_sc0007761.1_g000002
rosa_roxburghii Rroxscaffold_152G00434590 Rroxscaffold_1G00014400 Rroxscaffold_1G00014410 Rroxscaffold_1G00014570 Rroxscaffold_2G00100650 Rroxscaffold_2G00106640
rosa_rugosa Rorug02G0392200 Rorug02G0392400 Rorug05G0378500 Rorug05G0378500 Rorug05G0378500
rosa_samantha Rh2AG446400 Rh2AG446500 Rh2BG457100 Rh2BG457200 Rh2BG457300 Rh2BG458200 Rh2CG432200 Rh2CG433100 Rh2CG433200 Rh2DG466800 Rh2DG466900 Rh2DG467000 Rh2DG467800 Rh3DG193900 Rh5AG437600 Rh5AG437700 Rh5AG437800 Rh5BG453700 Rh5DG468800 Rh7AG352500 Rh7CG369800 Rh7DG349000
rosa_wichuraiana Rw2G036410 Rw2G036460 Rw3G014910 Rw5G040940 Rw7G029760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 515, 1563
AccB1I GGYRCC 2 cut(s) 322, 1451
AciI CCGC 1 cut(s) 790
AclI AACGTT 1 cut(s) 1590
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 4 cut(s) 478, 1369, 1514, 2076
AcuI CTGAAG 1 cut(s) 1833
AcvI CACGTG 1 cut(s) 1710
AfaI GTAC 4 cut(s) 723, 1596, 1646, 1774
AfiI CCNNNNNNNGG 5 cut(s) 596, 1122, 1342, 1506, 2095
AflII CTTAAG 1 cut(s) 451
AflIII ACRYGT 3 cut(s) 1638, 1707, 1709
AjnI CCWGG 6 cut(s) 277, 423, 595, 999, 1083, 1455
AloI GAACNNNNNNTCC 2 cut(s) 1512, 1544
Alw21I GWGCWC 2 cut(s) 1444, 1796
Alw26I GTCTC 3 cut(s) 244, 953, 1262
AlwI GGATC 1 cut(s) 587
AoxI GGCC 4 cut(s) 135, 349, 1268, 2086
ApeKI GCWGC 6 cut(s) 156, 1384, 1672, 1964, 2020, 2038
ApoI RAATTY 4 cut(s) 478, 1369, 1514, 2076
Asp700I GAANNNNTTC 1 cut(s) 1169
AspS9I GGNCC 6 cut(s) 350, 462, 599, 668, 1497, 2086
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 293
AsuII TTCGAA 1 cut(s) 876
AvaII GGWCC 4 cut(s) 462, 599, 668, 1497
BaeGI GKGCMC 3 cut(s) 325, 1456, 1664
BanI GGYRCC 2 cut(s) 322, 1451
BarI GAAGNNNNNNTAC 4 cut(s) 1013, 1045, 1316, 1348
BbrPI CACGTG 1 cut(s) 1710
BbsI GAAGAC 4 cut(s) 276, 387, 439, 997
Bbv12I GWGCWC 2 cut(s) 1444, 1796
BbvCI CCTCAGC 5 cut(s) 125, 645, 812, 1716, 1722
BbvI GCAGC 6 cut(s) 168, 1396, 1659, 1976, 2007, 2025
BccI CCATC 3 cut(s) 40, 146, 1816
BceAI ACGGC 1 cut(s) 1166
BciT130I CCWGG 6 cut(s) 279, 425, 597, 1001, 1085, 1457
BciVI GTATCC 1 cut(s) 746
BclI TGATCA 2 cut(s) 117, 1987
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 244, 953, 1262
BfaI CTAG 6 cut(s) 612, 665, 896, 924, 935, 1481
BfmI CTRYAG 2 cut(s) 766, 1133
BfrI CTTAAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 746
BglII AGATCT 1 cut(s) 1552
BisI GCNGC 7 cut(s) 157, 791, 1385, 1673, 1965, 2021, 2039
BlsI GCNGC 7 cut(s) 158, 792, 1386, 1674, 1966, 2022, 2040
BmcAI AGTACT 1 cut(s) 723
Bme1390I CCNGG 7 cut(s) 65, 279, 425, 597, 1001, 1085, 1457
Bme18I GGWCC 4 cut(s) 462, 599, 668, 1497
BmgT120I GGNCC 6 cut(s) 350, 462, 599, 668, 1497, 2086
BmiI GGNNCC 3 cut(s) 324, 601, 1453
BmrFI CCNGG 7 cut(s) 65, 279, 425, 597, 1001, 1085, 1457
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 7 cut(s) 42, 349, 700, 1411, 1459, 1536, 1840
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 4 cut(s) 276, 387, 439, 997
BpmI CTGGAG 3 cut(s) 181, 261, 2002
Bpu10I CCTNAGC 6 cut(s) 125, 645, 812, 1716, 1722, 2003
Bpu14I TTCGAA 1 cut(s) 876
BpuMI CCSGG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 1330
BsaAI YACGTR 1 cut(s) 1710
BsaJI CCNNGG 2 cut(s) 1455, 1881
BsaXI ACNNNNNCTCC 2 cut(s) 1029, 1059
Bsc4I CCNNNNNNNGG 5 cut(s) 596, 1122, 1342, 1506, 2095
Bse1I ACTGG 3 cut(s) 177, 603, 1127
BseBI CCWGG 6 cut(s) 279, 425, 597, 1001, 1085, 1457
BseCI ATCGAT 1 cut(s) 1330
BseDI CCNNGG 2 cut(s) 1455, 1881
BseGI GGATG 9 cut(s) 51, 138, 144, 586, 807, 1048, 1080, 1354, 1527
BseLI CCNNNNNNNGG 5 cut(s) 596, 1122, 1342, 1506, 2095
BseNI ACTGG 3 cut(s) 177, 603, 1127
BseRI GAGGAG 2 cut(s) 732, 2025
BseSI GKGCMC 3 cut(s) 325, 1456, 1664
BseXI GCAGC 6 cut(s) 168, 1396, 1659, 1976, 2007, 2025
BshFI GGCC 4 cut(s) 137, 351, 1270, 2088
BshNI GGYRCC 2 cut(s) 322, 1451
BshVI ATCGAT 1 cut(s) 1330
BsiHKAI GWGCWC 2 cut(s) 1444, 1796
BsiSI CCGG 2 cut(s) 65, 714
BslFI GGGAC 4 cut(s) 585, 1245, 1908, 1918
BslI CCNNNNNNNGG 5 cut(s) 596, 1122, 1342, 1506, 2095
BsmAI GTCTC 3 cut(s) 244, 953, 1262
BsmBI CGTCTC 1 cut(s) 953
BsmFI GGGAC 4 cut(s) 585, 1245, 1908, 1918
BsnI GGCC 4 cut(s) 137, 351, 1270, 2088
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 5 cut(s) 325, 1444, 1456, 1664, 1796
Bsp1407I TGTACA 1 cut(s) 1772
Bsp143I GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
Bsp19I CCATGG 1 cut(s) 1881
BspACI CCGC 1 cut(s) 790
BspANI GGCC 4 cut(s) 137, 351, 1270, 2088
BspDI ATCGAT 1 cut(s) 1330
BspLI GGNNCC 3 cut(s) 324, 601, 1453
BspPI GGATC 1 cut(s) 587
BspQI GCTCTTC 1 cut(s) 1175
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 2 cut(s) 322, 1451
BspTI CTTAAG 1 cut(s) 451
BsrGI TGTACA 1 cut(s) 1772
BsrI ACTGG 3 cut(s) 177, 603, 1127
BssECI CCNNGG 2 cut(s) 1455, 1881
BssMI GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
BssT1I CCWWGG 1 cut(s) 1881
Bst2UI CCWGG 6 cut(s) 279, 425, 597, 1001, 1085, 1457
Bst4CI ACNGT 5 cut(s) 238, 734, 770, 988, 2070
Bst6I CTCTTC 3 cut(s) 35, 1175, 1571
BstAFI CTTAAG 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 1772
BstBAI YACGTR 1 cut(s) 1710
BstBI TTCGAA 1 cut(s) 876
BstDSI CCRYGG 1 cut(s) 1881
BstF5I GGATG 9 cut(s) 51, 138, 144, 586, 807, 1048, 1080, 1354, 1527
BstKTI GATC 6 cut(s) 120, 595, 805, 1555, 1828, 1990
BstMAI GTCTC 3 cut(s) 244, 953, 1262
BstMBI GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
BstMWI GCNNNNNNNGC 4 cut(s) 320, 689, 1144, 2110
BstNI CCWGG 6 cut(s) 279, 425, 597, 1001, 1085, 1457
BstNSI RCATGY 3 cut(s) 1028, 1642, 1857
BstSCI CCNGG 7 cut(s) 63, 277, 423, 595, 999, 1083, 1455
BstSFI CTRYAG 2 cut(s) 766, 1133
BstSLI GKGCMC 3 cut(s) 325, 1456, 1664
BstV1I GCAGC 6 cut(s) 168, 1396, 1659, 1976, 2007, 2025
BstV2I GAAGAC 4 cut(s) 276, 387, 439, 997
BstX2I RGATCY 1 cut(s) 1552
BstYI RGATCY 1 cut(s) 1552
Bsu15I ATCGAT 1 cut(s) 1330
BsuI GTATCC 1 cut(s) 746
BsuRI GGCC 4 cut(s) 137, 351, 1270, 2088
BsuTUI ATCGAT 1 cut(s) 1330
BtgI CCRYGG 1 cut(s) 1881
BtgZI GCGATG 1 cut(s) 65
BtsCI GGATG 9 cut(s) 51, 138, 144, 586, 807, 1048, 1080, 1354, 1527
Cfr13I GGNCC 6 cut(s) 350, 462, 599, 668, 1497, 2086
ClaI ATCGAT 1 cut(s) 1330
Csp6I GTAC 4 cut(s) 722, 1595, 1645, 1773
CviQI GTAC 4 cut(s) 722, 1595, 1645, 1773
DpnI GATC 6 cut(s) 119, 594, 804, 1554, 1827, 1989
DpnII GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
DraI TTTAAA 1 cut(s) 756
Eam1104I CTCTTC 3 cut(s) 35, 1175, 1571
EarI CTCTTC 3 cut(s) 35, 1175, 1571
Eco130I CCWWGG 1 cut(s) 1881
Eco47I GGWCC 4 cut(s) 462, 599, 668, 1497
Eco57I CTGAAG 1 cut(s) 1833
Eco72I CACGTG 1 cut(s) 1710
EcoRII CCWGG 6 cut(s) 277, 423, 595, 999, 1083, 1455
EcoT14I CCWWGG 1 cut(s) 1881
EcoT22I ATGCAT 2 cut(s) 1030, 1855
ErhI CCWWGG 1 cut(s) 1881
Esp3I CGTCTC 1 cut(s) 953
FalI AAGNNNNNCTT 2 cut(s) 1283, 1315
FaqI GGGAC 4 cut(s) 585, 1245, 1908, 1918
FauNDI CATATG 1 cut(s) 1057
FbaI TGATCA 2 cut(s) 117, 1987
Fnu4HI GCNGC 7 cut(s) 157, 791, 1385, 1673, 1965, 2021, 2039
FokI GGATG 9 cut(s) 58, 125, 131, 573, 794, 1055, 1067, 1361, 1514
Fsp4HI GCNGC 7 cut(s) 157, 791, 1385, 1673, 1965, 2021, 2039
FspBI CTAG 6 cut(s) 612, 665, 896, 924, 935, 1481
GluI GCNGC 7 cut(s) 157, 791, 1385, 1673, 1965, 2021, 2039
GsuI CTGGAG 3 cut(s) 181, 261, 2002
HaeIII GGCC 4 cut(s) 137, 351, 1270, 2088
HapII CCGG 2 cut(s) 65, 714
HindIII AAGCTT 3 cut(s) 870, 1767, 1869
HinfI GANTC 7 cut(s) 224, 272, 284, 572, 651, 900, 1340
HpaII CCGG 2 cut(s) 65, 714
HphI GGTGA 1 cut(s) 293
Hpy188I TCNGA 7 cut(s) 191, 865, 1120, 1345, 1633, 1813, 2083
Hpy188III TCNNGA 6 cut(s) 85, 296, 590, 1466, 1703, 2005
Hpy99I CGWCG 1 cut(s) 1760
HpyAV CCTTC 4 cut(s) 1288, 1541, 1675, 1813
HpyCH4III ACNGT 5 cut(s) 238, 734, 770, 988, 2070
HpyCH4IV ACGT 2 cut(s) 1590, 1709
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 689, 1144, 2110
HpySE526I ACGT 2 cut(s) 1590, 1709
Ksp22I TGATCA 2 cut(s) 117, 1987
Kzo9I GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
LguI GCTCTTC 1 cut(s) 1175
LmnI GCTCC 3 cut(s) 97, 688, 1073
Lsp1109I GCAGC 6 cut(s) 168, 1396, 1659, 1976, 2007, 2025
LweI GCATC 7 cut(s) 42, 349, 700, 1411, 1459, 1536, 1840
MaeI CTAG 6 cut(s) 612, 665, 896, 924, 935, 1481
MaeII ACGT 2 cut(s) 1590, 1709
MaeIII GTNAC 1 cut(s) 179
MalI GATC 6 cut(s) 119, 594, 804, 1554, 1827, 1989
MboI GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
MflI RGATCY 1 cut(s) 1552
MhlI GDGCHC 5 cut(s) 325, 1444, 1456, 1664, 1796
MlyI GAGTC 4 cut(s) 218, 266, 581, 1334
MmeI TCCRAC 3 cut(s) 282, 729, 1611
Mph1103I ATGCAT 2 cut(s) 1030, 1855
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 5 cut(s) 452, 542, 755, 1203, 1290
MslI CAYNNNNRTG 2 cut(s) 345, 1152
MspA1I CMGCKG 2 cut(s) 159, 1138
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 2 cut(s) 65, 714
MspR9I CCNGG 7 cut(s) 65, 279, 425, 597, 1001, 1085, 1457
MvaI CCWGG 6 cut(s) 279, 425, 597, 1001, 1085, 1457
MwoI GCNNNNNNNGC 4 cut(s) 320, 689, 1144, 2110
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 1881
NdeI CATATG 1 cut(s) 1057
NdeII GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
NlaIV GGNNCC 3 cut(s) 324, 601, 1453
NsiI ATGCAT 2 cut(s) 1030, 1855
NspI RCATGY 3 cut(s) 1028, 1642, 1857
NspV TTCGAA 1 cut(s) 876
PaeI GCATGC 2 cut(s) 1028, 1857
PciI ACATGT 1 cut(s) 1638
PciSI GCTCTTC 1 cut(s) 1175
PdmI GAANNNNTTC 1 cut(s) 1169
PfeI GAWTC 3 cut(s) 284, 651, 900
PfoI TCCNGGA 2 cut(s) 63, 1083
PkrI GCNGC 7 cut(s) 158, 792, 1386, 1674, 1966, 2022, 2040
PleI GAGTC 4 cut(s) 218, 266, 580, 1334
PmaCI CACGTG 1 cut(s) 1710
PmlI CACGTG 1 cut(s) 1710
PpsI GAGTC 4 cut(s) 218, 266, 580, 1334
Ppu21I YACGTR 1 cut(s) 1710
PscI ACATGT 1 cut(s) 1638
PsiI TTATAA 2 cut(s) 515, 1563
Psp1406I AACGTT 1 cut(s) 1590
Psp6I CCWGG 6 cut(s) 277, 423, 595, 999, 1083, 1455
PspCI CACGTG 1 cut(s) 1710
PspGI CCWGG 6 cut(s) 277, 423, 595, 999, 1083, 1455
PspN4I GGNNCC 3 cut(s) 324, 601, 1453
PspPI GGNCC 6 cut(s) 350, 462, 599, 668, 1497, 2086
PsuI RGATCY 1 cut(s) 1552
PvuII CAGCTG 2 cut(s) 159, 1138
RsaI GTAC 4 cut(s) 723, 1596, 1646, 1774
RsaNI GTAC 4 cut(s) 722, 1595, 1645, 1773
RseI CAYNNNNRTG 2 cut(s) 345, 1152
SapI GCTCTTC 1 cut(s) 1175
SaqAI TTAA 5 cut(s) 452, 542, 755, 1203, 1290
SatI GCNGC 7 cut(s) 157, 791, 1385, 1673, 1965, 2021, 2039
Sau3AI GATC 6 cut(s) 117, 592, 802, 1552, 1825, 1987
Sau96I GGNCC 6 cut(s) 350, 462, 599, 668, 1497, 2086
ScaI AGTACT 1 cut(s) 723
SchI GAGTC 4 cut(s) 218, 266, 581, 1334
ScrFI CCNGG 7 cut(s) 65, 279, 425, 597, 1001, 1085, 1457
SduI GDGCHC 5 cut(s) 325, 1444, 1456, 1664, 1796
SfaNI GCATC 7 cut(s) 42, 349, 700, 1411, 1459, 1536, 1840
SfcI CTRYAG 2 cut(s) 766, 1133
SfuI TTCGAA 1 cut(s) 876
SinI GGWCC 4 cut(s) 462, 599, 668, 1497
SmiMI CAYNNNNRTG 2 cut(s) 345, 1152
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
SphI GCATGC 2 cut(s) 1028, 1857
SsiI CCGC 1 cut(s) 790
SspMI CTAG 6 cut(s) 612, 665, 896, 924, 935, 1481
StyD4I CCNGG 7 cut(s) 63, 277, 423, 595, 999, 1083, 1455
StyI CCWWGG 1 cut(s) 1881
TaaI ACNGT 5 cut(s) 238, 734, 770, 988, 2070
TaiI ACGT 2 cut(s) 1593, 1712
TaqI TCGA 5 cut(s) 876, 1330, 1465, 1586, 1828
TatI WGTACW 2 cut(s) 721, 1772
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 3 cut(s) 284, 651, 900
Tru1I TTAA 5 cut(s) 452, 542, 755, 1203, 1290
Tru9I TTAA 5 cut(s) 452, 542, 755, 1203, 1290
TseI GCWGC 6 cut(s) 156, 1384, 1672, 1964, 2020, 2038
TspDTI ATGAA 7 cut(s) 402, 490, 892, 929, 1176, 1294, 1657
TspGWI ACGGA 1 cut(s) 953
Vha464I CTTAAG 1 cut(s) 451
VpaK11BI GGWCC 4 cut(s) 462, 599, 668, 1497
XapI RAATTY 4 cut(s) 478, 1369, 1514, 2076
XceI RCATGY 3 cut(s) 1028, 1642, 1857
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 6 cut(s) 612, 665, 896, 924, 935, 1481
ZrmI AGTACT 1 cut(s) 723
Zsp2I ATGCAT 2 cut(s) 1030, 1855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.