Domain of unknown function (DUF4220)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 57 |
| AciI | CCGC | 1 cut(s) | 55 |
| AclWI | GGATC | 1 cut(s) | 11 |
| AcuI | CTGAAG | 2 cut(s) | 90, 158 |
| AfaI | GTAC | 1 cut(s) | 277 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 20, 61 |
| AgsI | TTSAA | 5 cut(s) | 79, 104, 157, 180, 202 |
| AjnI | CCWGG | 1 cut(s) | 19 |
| AluBI | AGCT | 1 cut(s) | 223 |
| AluI | AGCT | 1 cut(s) | 223 |
| AlwI | GGATC | 1 cut(s) | 11 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 1 cut(s) | 22 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 50 |
| AspS9I | GGNCC | 2 cut(s) | 23, 118 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| AvaII | GGWCC | 1 cut(s) | 118 |
| BciT130I | CCWGG | 1 cut(s) | 21 |
| BclI | TGATCA | 1 cut(s) | 217 |
| Bme1390I | CCNGG | 1 cut(s) | 21 |
| Bme18I | GGWCC | 1 cut(s) | 118 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmgT120I | GGNCC | 2 cut(s) | 23, 118 |
| BmiI | GGNNCC | 1 cut(s) | 25 |
| BmrFI | CCNGG | 1 cut(s) | 21 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 41, 73 |
| Bsa29I | ATCGAT | 1 cut(s) | 174 |
| BsaBI | GATNNNNATC | 1 cut(s) | 189 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 20, 61 |
| Bse8I | GATNNNNATC | 1 cut(s) | 189 |
| BseBI | CCWGG | 1 cut(s) | 21 |
| BseCI | ATCGAT | 1 cut(s) | 174 |
| BseGI | GGATG | 1 cut(s) | 190 |
| BseJI | GATNNNNATC | 1 cut(s) | 189 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 20, 61 |
| BshFI | GGCC | 1 cut(s) | 24 |
| BshVI | ATCGAT | 1 cut(s) | 174 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 20, 61 |
| BsnI | GGCC | 1 cut(s) | 24 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp143I | GATC | 2 cut(s) | 16, 217 |
| BspACI | CCGC | 1 cut(s) | 55 |
| BspANI | GGCC | 1 cut(s) | 24 |
| BspDI | ATCGAT | 1 cut(s) | 174 |
| BspLI | GGNNCC | 1 cut(s) | 25 |
| BspPI | GGATC | 1 cut(s) | 11 |
| BsrBI | CCGCTC | 1 cut(s) | 57 |
| BssMI | GATC | 2 cut(s) | 16, 217 |
| Bst2UI | CCWGG | 1 cut(s) | 21 |
| BstDEI | CTNAG | 1 cut(s) | 60 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 18 |
| BstF5I | GGATG | 1 cut(s) | 190 |
| BstKTI | GATC | 2 cut(s) | 19, 220 |
| BstMBI | GATC | 2 cut(s) | 16, 217 |
| BstNI | CCWGG | 1 cut(s) | 21 |
| BstSCI | CCNGG | 1 cut(s) | 19 |
| Bsu15I | ATCGAT | 1 cut(s) | 174 |
| BsuRI | GGCC | 1 cut(s) | 24 |
| BsuTUI | ATCGAT | 1 cut(s) | 174 |
| BtsCI | GGATG | 1 cut(s) | 190 |
| Cfr13I | GGNCC | 2 cut(s) | 23, 118 |
| ClaI | ATCGAT | 1 cut(s) | 174 |
| Csp6I | GTAC | 1 cut(s) | 276 |
| CviAII | CATG | 2 cut(s) | 43, 317 |
| CviJI | RGCY | 3 cut(s) | 24, 75, 223 |
| CviKI_1 | RGCY | 3 cut(s) | 24, 75, 223 |
| CviQI | GTAC | 1 cut(s) | 276 |
| DdeI | CTNAG | 1 cut(s) | 60 |
| DpnI | GATC | 2 cut(s) | 18, 219 |
| DpnII | GATC | 2 cut(s) | 16, 217 |
| Eco47I | GGWCC | 1 cut(s) | 118 |
| Eco57I | CTGAAG | 2 cut(s) | 90, 158 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 18 |
| EcoO109I | RGGNCCY | 1 cut(s) | 23 |
| EcoRII | CCWGG | 1 cut(s) | 19 |
| FaeI | CATG | 2 cut(s) | 46, 320 |
| FaiI | YATR | 7 cut(s) | 44, 127, 145, 147, 274, 282, 318 |
| FatI | CATG | 2 cut(s) | 42, 316 |
| FbaI | TGATCA | 1 cut(s) | 217 |
| FokI | GGATG | 1 cut(s) | 197 |
| HaeIII | GGCC | 1 cut(s) | 24 |
| Hin1II | CATG | 2 cut(s) | 46, 320 |
| HinfI | GANTC | 3 cut(s) | 65, 176, 295 |
| Hpy188I | TCNGA | 1 cut(s) | 70 |
| Hpy188III | TCNNGA | 5 cut(s) | 14, 121, 194, 292, 299 |
| HpyAV | CCTTC | 1 cut(s) | 65 |
| HpyCH4V | TGCA | 1 cut(s) | 264 |
| HpyF3I | CTNAG | 1 cut(s) | 60 |
| Hsp92II | CATG | 2 cut(s) | 46, 320 |
| Ksp22I | TGATCA | 1 cut(s) | 217 |
| Kzo9I | GATC | 2 cut(s) | 16, 217 |
| LmnI | GCTCC | 1 cut(s) | 9 |
| LpnPI | CCDG | 8 cut(s) | 6, 27, 33, 49, 134, 179, 261, 277 |
| MalI | GATC | 2 cut(s) | 18, 219 |
| MbiI | CCGCTC | 1 cut(s) | 57 |
| MboI | GATC | 2 cut(s) | 16, 217 |
| MboII | GAAGA | 1 cut(s) | 151 |
| MluCI | AATT | 2 cut(s) | 206, 269 |
| MnlI | CCTC | 3 cut(s) | 18, 21, 250 |
| MroXI | GAANNNNTTC | 1 cut(s) | 50 |
| MslI | CAYNNNNRTG | 1 cut(s) | 285 |
| MspR9I | CCNGG | 1 cut(s) | 21 |
| MvaI | CCWGG | 1 cut(s) | 21 |
| NdeII | GATC | 2 cut(s) | 16, 217 |
| NlaIII | CATG | 2 cut(s) | 46, 320 |
| NlaIV | GGNNCC | 1 cut(s) | 25 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PdmI | GAANNNNTTC | 1 cut(s) | 50 |
| PfeI | GAWTC | 3 cut(s) | 65, 176, 295 |
| Psp6I | CCWGG | 1 cut(s) | 19 |
| PspGI | CCWGG | 1 cut(s) | 19 |
| PspN4I | GGNNCC | 1 cut(s) | 25 |
| PspPI | GGNCC | 2 cut(s) | 23, 118 |
| PspXI | VCTCGAGB | 1 cut(s) | 254 |
| RsaI | GTAC | 1 cut(s) | 277 |
| RsaNI | GTAC | 1 cut(s) | 276 |
| RseI | CAYNNNNRTG | 1 cut(s) | 285 |
| Sau3AI | GATC | 2 cut(s) | 16, 217 |
| Sau96I | GGNCC | 2 cut(s) | 23, 118 |
| ScrFI | CCNGG | 1 cut(s) | 21 |
| SetI | ASST | 4 cut(s) | 84, 120, 168, 225 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SinI | GGWCC | 1 cut(s) | 118 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 285 |
| SmlI | CTYRAG | 1 cut(s) | 254 |
| SmoI | CTYRAG | 1 cut(s) | 254 |
| Sse9I | AATT | 2 cut(s) | 206, 269 |
| SsiI | CCGC | 1 cut(s) | 55 |
| StyD4I | CCNGG | 1 cut(s) | 19 |
| TaqI | TCGA | 2 cut(s) | 174, 255 |
| TasI | AATT | 2 cut(s) | 206, 269 |
| TatI | WGTACW | 1 cut(s) | 275 |
| TfiI | GAWTC | 3 cut(s) | 65, 176, 295 |
| TspDTI | ATGAA | 1 cut(s) | 59 |
| VpaK11BI | GGWCC | 1 cut(s) | 118 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 18 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XmnI | GAANNNNTTC | 1 cut(s) | 50 |