Domain of unknown function (DUF4220)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1419 |
| AatII | GACGTC | 1 cut(s) | 180 |
| AccB1I | GGYRCC | 1 cut(s) | 403 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1188, 2174 |
| AccI | GTMKAC | 1 cut(s) | 1409 |
| AccII | CGCG | 2 cut(s) | 320, 2204 |
| AciI | CCGC | 5 cut(s) | 45, 429, 553, 625, 1865 |
| AclWI | GGATC | 5 cut(s) | 266, 272, 785, 798, 1023 |
| AcuI | CTGAAG | 5 cut(s) | 786, 825, 832, 1018, 1416 |
| AcvI | CACGTG | 1 cut(s) | 2159 |
| AcyI | GRCGYC | 1 cut(s) | 177 |
| AfaI | GTAC | 4 cut(s) | 248, 573, 757, 2140 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 11, 391, 677, 1188, 1864, 2119, 2174 |
| AflIII | ACRYGT | 2 cut(s) | 1781, 2156 |
| AhlI | ACTAGT | 1 cut(s) | 1174 |
| AjnI | CCWGG | 1 cut(s) | 504 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 942, 974 |
| AleI | CACNNNNGTG | 1 cut(s) | 1061 |
| Alw21I | GWGCWC | 3 cut(s) | 44, 423, 2067 |
| Alw26I | GTCTC | 2 cut(s) | 117, 1770 |
| Alw44I | GTGCAC | 1 cut(s) | 419 |
| AlwI | GGATC | 5 cut(s) | 266, 272, 785, 798, 1023 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 764 |
| Ama87I | CYCGRG | 1 cut(s) | 729 |
| AoxI | GGCC | 5 cut(s) | 285, 916, 1656, 1791, 1854 |
| ApaLI | GTGCAC | 1 cut(s) | 419 |
| ApeKI | GCWGC | 5 cut(s) | 311, 1047, 2039, 2074, 2095 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 162, 194 |
| AspLEI | GCGC | 4 cut(s) | 322, 1734, 2204, 2206 |
| AspS9I | GGNCC | 4 cut(s) | 431, 619, 1855, 1969 |
| AsuC2I | CCSGG | 2 cut(s) | 182, 2197 |
| AsuHPI | GGTGA | 3 cut(s) | 746, 1879, 2157 |
| AsuII | TTCGAA | 1 cut(s) | 1926 |
| AvaI | CYCGRG | 1 cut(s) | 729 |
| AvaII | GGWCC | 3 cut(s) | 431, 619, 1969 |
| BaeGI | GKGCMC | 3 cut(s) | 406, 423, 1886 |
| BamHI | GGATCC | 1 cut(s) | 790 |
| BanI | GGYRCC | 1 cut(s) | 403 |
| BanII | GRGCYC | 1 cut(s) | 44 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1920, 1952 |
| BauI | CACGAG | 1 cut(s) | 1010 |
| BbrPI | CACGTG | 1 cut(s) | 2159 |
| BbsI | GAAGAC | 1 cut(s) | 928 |
| Bbv12I | GWGCWC | 3 cut(s) | 44, 423, 2067 |
| BbvI | GCAGC | 5 cut(s) | 298, 1034, 2051, 2082, 2086 |
| BccI | CCATC | 4 cut(s) | 25, 277, 1172, 1971 |
| BceAI | ACGGC | 1 cut(s) | 1460 |
| BciT130I | CCWGG | 1 cut(s) | 506 |
| BclI | TGATCA | 4 cut(s) | 355, 387, 1648, 1686 |
| BcnI | CCSGG | 2 cut(s) | 182, 2197 |
| BcoDI | GTCTC | 2 cut(s) | 117, 1770 |
| BcuI | ACTAGT | 1 cut(s) | 1174 |
| BfaI | CTAG | 5 cut(s) | 491, 753, 1175, 1226, 2212 |
| BfmI | CTRYAG | 5 cut(s) | 111, 312, 1473, 1527, 1627 |
| BglII | AGATCT | 1 cut(s) | 843 |
| BisI | GCNGC | 5 cut(s) | 312, 1048, 2040, 2075, 2096 |
| BlsI | GCNGC | 5 cut(s) | 313, 1049, 2041, 2076, 2097 |
| Bme1390I | CCNGG | 3 cut(s) | 182, 506, 2197 |
| Bme18I | GGWCC | 3 cut(s) | 431, 619, 1969 |
| BmeT110I | CYCGRG | 1 cut(s) | 729 |
| BmgT120I | GGNCC | 4 cut(s) | 431, 619, 1855, 1969 |
| BmiI | GGNNCC | 3 cut(s) | 405, 620, 792 |
| BmrFI | CCNGG | 3 cut(s) | 182, 506, 2197 |
| BmrI | ACTGGG | 1 cut(s) | 313 |
| BmsI | GCATC | 2 cut(s) | 862, 1459 |
| BmuI | ACTGGG | 1 cut(s) | 313 |
| BoxI | GACNNNNGTC | 3 cut(s) | 617, 1113, 1329 |
| BpiI | GAAGAC | 1 cut(s) | 928 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2078 |
| Bpu14I | TTCGAA | 1 cut(s) | 1926 |
| BpuEI | CTTGAG | 1 cut(s) | 1172 |
| BpuMI | CCSGG | 2 cut(s) | 182, 2197 |
| BsaAI | YACGTR | 1 cut(s) | 2159 |
| BsaBI | GATNNNNATC | 2 cut(s) | 263, 354 |
| BsaHI | GRCGYC | 1 cut(s) | 177 |
| BsaJI | CCNNGG | 5 cut(s) | 10, 343, 424, 1302, 2195 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 11, 391, 677, 1188, 1864, 2119, 2174 |
| Bse118I | RCCGGY | 1 cut(s) | 1971 |
| Bse1I | ACTGG | 3 cut(s) | 308, 575, 1965 |
| Bse3DI | GCAATG | 1 cut(s) | 1285 |
| Bse8I | GATNNNNATC | 2 cut(s) | 263, 354 |
| BseBI | CCWGG | 1 cut(s) | 506 |
| BseDI | CCNNGG | 5 cut(s) | 10, 343, 424, 1302, 2195 |
| BseGI | GGATG | 3 cut(s) | 288, 373, 870 |
| BseJI | GATNNNNATC | 2 cut(s) | 263, 354 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 11, 391, 677, 1188, 1864, 2119, 2174 |
| BseMI | GCAATG | 1 cut(s) | 1285 |
| BseMII | CTCAG | 1 cut(s) | 120 |
| BseNI | ACTGG | 3 cut(s) | 308, 575, 1965 |
| BsePI | GCGCGC | 1 cut(s) | 2202 |
| BseRI | GAGGAG | 1 cut(s) | 2105 |
| BseSI | GKGCMC | 3 cut(s) | 406, 423, 1886 |
| BseXI | GCAGC | 5 cut(s) | 298, 1034, 2051, 2082, 2086 |
| BseYI | CCCAGC | 2 cut(s) | 254, 1885 |
| Bsh1236I | CGCG | 2 cut(s) | 320, 2204 |
| BshFI | GGCC | 5 cut(s) | 287, 918, 1658, 1793, 1856 |
| BshNI | GGYRCC | 1 cut(s) | 403 |
| BsiHKAI | GWGCWC | 3 cut(s) | 44, 423, 2067 |
| BsiHKCI | CYCGRG | 1 cut(s) | 729 |
| BsiSI | CCGG | 3 cut(s) | 181, 1972, 2197 |
| BslFI | GGGAC | 2 cut(s) | 188, 2174 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 11, 391, 677, 1188, 1864, 2119, 2174 |
| BsmAI | GTCTC | 2 cut(s) | 117, 1770 |
| BsmFI | GGGAC | 2 cut(s) | 188, 2174 |
| BsmI | GAATGC | 2 cut(s) | 1003, 1590 |
| BsnI | GGCC | 5 cut(s) | 287, 918, 1658, 1793, 1856 |
| BsoBI | CYCGRG | 1 cut(s) | 729 |
| Bsp119I | TTCGAA | 1 cut(s) | 1926 |
| Bsp1286I | GDGCHC | 5 cut(s) | 44, 406, 423, 1886, 2067 |
| Bsp19I | CCATGG | 2 cut(s) | 10, 1302 |
| BspACI | CCGC | 5 cut(s) | 45, 429, 553, 625, 1865 |
| BspANI | GGCC | 5 cut(s) | 287, 918, 1658, 1793, 1856 |
| BspCNI | CTCAG | 1 cut(s) | 119 |
| BspFNI | CGCG | 2 cut(s) | 320, 2204 |
| BspHI | TCATGA | 1 cut(s) | 1167 |
| BspLI | GGNNCC | 3 cut(s) | 405, 620, 792 |
| BspMAI | CTGCAG | 1 cut(s) | 316 |
| BspPI | GGATC | 5 cut(s) | 266, 272, 785, 798, 1023 |
| BspT104I | TTCGAA | 1 cut(s) | 1926 |
| BspT107I | GGYRCC | 1 cut(s) | 403 |
| BsrDI | GCAATG | 1 cut(s) | 1285 |
| BsrFI | RCCGGY | 1 cut(s) | 1971 |
| BsrI | ACTGG | 3 cut(s) | 308, 575, 1965 |
| BssAI | RCCGGY | 1 cut(s) | 1971 |
| BssECI | CCNNGG | 5 cut(s) | 10, 343, 424, 1302, 2195 |
| BssHII | GCGCGC | 1 cut(s) | 2202 |
| BssNAI | GTATAC | 1 cut(s) | 1410 |
| BssNI | GRCGYC | 1 cut(s) | 177 |
| BssSI | CACGAG | 1 cut(s) | 1010 |
| BssT1I | CCWWGG | 4 cut(s) | 10, 343, 424, 1302 |
| Bst1107I | GTATAC | 1 cut(s) | 1410 |
| Bst2BI | CACGAG | 1 cut(s) | 1010 |
| Bst2UI | CCWGG | 1 cut(s) | 506 |
| Bst4CI | ACNGT | 6 cut(s) | 69, 760, 781, 1477, 1918, 2069 |
| Bst6I | CTCTTC | 1 cut(s) | 924 |
| BstACI | GRCGYC | 1 cut(s) | 177 |
| BstBAI | YACGTR | 1 cut(s) | 2159 |
| BstBI | TTCGAA | 1 cut(s) | 1926 |
| BstC8I | GCNNGC | 6 cut(s) | 634, 1040, 1211, 1656, 2044, 2204 |
| BstDEI | CTNAG | 4 cut(s) | 106, 647, 1830, 2078 |
| BstDSI | CCRYGG | 2 cut(s) | 10, 1302 |
| BstF5I | GGATG | 3 cut(s) | 288, 373, 870 |
| BstFNI | CGCG | 2 cut(s) | 320, 2204 |
| BstHHI | GCGC | 4 cut(s) | 322, 1734, 2204, 2206 |
| BstMAI | GTCTC | 2 cut(s) | 117, 1770 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 317, 401, 701, 725, 1044, 1853, 1862, 2071, 2080 |
| BstNI | CCWGG | 1 cut(s) | 506 |
| BstNSI | RCATGY | 1 cut(s) | 1785 |
| BstPAI | GACNNNNGTC | 3 cut(s) | 617, 1113, 1329 |
| BstSCI | CCNGG | 3 cut(s) | 180, 504, 2195 |
| BstSFI | CTRYAG | 5 cut(s) | 111, 312, 1473, 1527, 1627 |
| BstSLI | GKGCMC | 3 cut(s) | 406, 423, 1886 |
| BstUI | CGCG | 2 cut(s) | 320, 2204 |
| BstV1I | GCAGC | 5 cut(s) | 298, 1034, 2051, 2082, 2086 |
| BstV2I | GAAGAC | 1 cut(s) | 928 |
| BstX2I | RGATCY | 3 cut(s) | 258, 790, 843 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1063 |
| BstYI | RGATCY | 3 cut(s) | 258, 790, 843 |
| BstZ17I | GTATAC | 1 cut(s) | 1410 |
| BsuRI | GGCC | 5 cut(s) | 287, 918, 1658, 1793, 1856 |
| BtgI | CCRYGG | 2 cut(s) | 10, 1302 |
| BtgZI | GCGATG | 1 cut(s) | 1990 |
| BtsCI | GGATG | 3 cut(s) | 288, 373, 870 |
| BtsI | GCAGTG | 1 cut(s) | 249 |
| BtsIMutI | CAGTG | 6 cut(s) | 249, 582, 786, 1482, 1923, 1958 |
| Cac8I | GCNNGC | 6 cut(s) | 634, 1040, 1211, 1656, 2044, 2204 |
| CaiI | CAGNNNCTG | 1 cut(s) | 764 |
| CciI | TCATGA | 1 cut(s) | 1167 |
| CfoI | GCGC | 4 cut(s) | 322, 1734, 2204, 2206 |
| Cfr10I | RCCGGY | 1 cut(s) | 1971 |
| Cfr13I | GGNCC | 4 cut(s) | 431, 619, 1855, 1969 |
| Csp6I | GTAC | 4 cut(s) | 247, 572, 756, 2139 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 871, 906 |
| CviQI | GTAC | 4 cut(s) | 247, 572, 756, 2139 |
| DdeI | CTNAG | 4 cut(s) | 106, 647, 1830, 2078 |
| Eam1104I | CTCTTC | 1 cut(s) | 924 |
| EarI | CTCTTC | 1 cut(s) | 924 |
| Ecl136II | GAGCTC | 1 cut(s) | 42 |
| Eco130I | CCWWGG | 4 cut(s) | 10, 343, 424, 1302 |
| Eco147I | AGGCCT | 1 cut(s) | 1658 |
| Eco24I | GRGCYC | 1 cut(s) | 44 |
| Eco47I | GGWCC | 3 cut(s) | 431, 619, 1969 |
| Eco53kI | GAGCTC | 1 cut(s) | 42 |
| Eco57I | CTGAAG | 5 cut(s) | 786, 825, 832, 1018, 1416 |
| Eco72I | CACGTG | 1 cut(s) | 2159 |
| Eco88I | CYCGRG | 1 cut(s) | 729 |
| EcoICRI | GAGCTC | 1 cut(s) | 42 |
| EcoO109I | RGGNCCY | 2 cut(s) | 619, 1855 |
| EcoRII | CCWGG | 1 cut(s) | 504 |
| EcoT14I | CCWWGG | 4 cut(s) | 10, 343, 424, 1302 |
| EcoT38I | GRGCYC | 1 cut(s) | 44 |
| ErhI | CCWWGG | 4 cut(s) | 10, 343, 424, 1302 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1105, 1137 |
| FaqI | GGGAC | 2 cut(s) | 188, 2174 |
| FauI | CCCGC | 1 cut(s) | 560 |
| FbaI | TGATCA | 4 cut(s) | 355, 387, 1648, 1686 |
| FblI | GTMKAC | 1 cut(s) | 1409 |
| Fnu4HI | GCNGC | 5 cut(s) | 312, 1048, 2040, 2075, 2096 |
| FokI | GGATG | 3 cut(s) | 295, 360, 857 |
| FriOI | GRGCYC | 1 cut(s) | 44 |
| Fsp4HI | GCNGC | 5 cut(s) | 312, 1048, 2040, 2075, 2096 |
| FspBI | CTAG | 5 cut(s) | 491, 753, 1175, 1226, 2212 |
| GlaI | GCGC | 4 cut(s) | 321, 1733, 2203, 2205 |
| GluI | GCNGC | 5 cut(s) | 312, 1048, 2040, 2075, 2096 |
| GsaI | CCCAGC | 2 cut(s) | 258, 1889 |
| HaeIII | GGCC | 5 cut(s) | 287, 918, 1658, 1793, 1856 |
| HapII | CCGG | 3 cut(s) | 181, 1972, 2197 |
| HhaI | GCGC | 4 cut(s) | 322, 1734, 2204, 2206 |
| Hin1I | GRCGYC | 1 cut(s) | 177 |
| Hin6I | GCGC | 4 cut(s) | 320, 1732, 2202, 2204 |
| HinP1I | GCGC | 4 cut(s) | 320, 1732, 2202, 2204 |
| HindIII | AAGCTT | 1 cut(s) | 644 |
| HinfI | GANTC | 5 cut(s) | 653, 1298, 1564, 1673, 1770 |
| HpaII | CCGG | 3 cut(s) | 181, 1972, 2197 |
| HphI | GGTGA | 3 cut(s) | 746, 1879, 2157 |
| Hpy166II | GTNNAC | 5 cut(s) | 249, 421, 1410, 1870, 2141 |
| Hpy188III | TCNNGA | 7 cut(s) | 262, 434, 529, 841, 1151, 1168, 1637 |
| Hpy8I | GTNNAC | 5 cut(s) | 249, 421, 1410, 1870, 2141 |
| Hpy99I | CGWCG | 1 cut(s) | 243 |
| HpyAV | CCTTC | 6 cut(s) | 761, 1042, 1391, 1868, 1922, 2053 |
| HpyCH4III | ACNGT | 6 cut(s) | 69, 760, 781, 1477, 1918, 2069 |
| HpyCH4IV | ACGT | 3 cut(s) | 177, 889, 2158 |
| HpyCH4V | TGCA | 8 cut(s) | 314, 421, 632, 784, 1003, 1050, 1935, 2039 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 317, 401, 701, 725, 1044, 1853, 1862, 2071, 2080 |
| HpyF3I | CTNAG | 4 cut(s) | 106, 647, 1830, 2078 |
| HpySE526I | ACGT | 3 cut(s) | 177, 889, 2158 |
| Hsp92I | GRCGYC | 1 cut(s) | 177 |
| HspAI | GCGC | 4 cut(s) | 320, 1732, 2202, 2204 |
| Ksp22I | TGATCA | 4 cut(s) | 355, 387, 1648, 1686 |
| LmnI | GCTCC | 6 cut(s) | 39, 47, 1139, 2062, 2092, 2199 |
| Lsp1109I | GCAGC | 5 cut(s) | 298, 1034, 2051, 2082, 2086 |
| LweI | GCATC | 2 cut(s) | 862, 1459 |
| MaeI | CTAG | 5 cut(s) | 491, 753, 1175, 1226, 2212 |
| MaeII | ACGT | 3 cut(s) | 177, 889, 2158 |
| MaeIII | GTNAC | 5 cut(s) | 274, 650, 760, 1956, 1982 |
| MboII | GAAGA | 7 cut(s) | 40, 404, 838, 928, 941, 1586, 1712 |
| MfeI | CAATTG | 1 cut(s) | 993 |
| MflI | RGATCY | 3 cut(s) | 258, 790, 843 |
| MhlI | GDGCHC | 5 cut(s) | 44, 406, 423, 1886, 2067 |
| MlyI | GAGTC | 4 cut(s) | 647, 1307, 1573, 1682 |
| MmeI | TCCRAC | 3 cut(s) | 17, 490, 1541 |
| MseI | TTAA | 3 cut(s) | 479, 497, 1505 |
| MslI | CAYNNNNRTG | 2 cut(s) | 281, 1061 |
| MspA1I | CMGCKG | 2 cut(s) | 110, 1061 |
| MspI | CCGG | 3 cut(s) | 181, 1972, 2197 |
| MspR9I | CCNGG | 3 cut(s) | 182, 506, 2197 |
| MunI | CAATTG | 1 cut(s) | 993 |
| Mva1269I | GAATGC | 2 cut(s) | 1003, 1590 |
| MvaI | CCWGG | 1 cut(s) | 506 |
| MvnI | CGCG | 2 cut(s) | 320, 2204 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 317, 401, 701, 725, 1044, 1853, 1862, 2071, 2080 |
| NciI | CCSGG | 2 cut(s) | 182, 2197 |
| NcoI | CCATGG | 2 cut(s) | 10, 1302 |
| NlaIV | GGNNCC | 3 cut(s) | 405, 620, 792 |
| NmuCI | GTSAC | 3 cut(s) | 274, 650, 1956 |
| NspI | RCATGY | 1 cut(s) | 1785 |
| NspV | TTCGAA | 1 cut(s) | 1926 |
| OliI | CACNNNNGTG | 1 cut(s) | 1061 |
| PaeR7I | CTCGAG | 1 cut(s) | 729 |
| PagI | TCATGA | 1 cut(s) | 1167 |
| PauI | GCGCGC | 1 cut(s) | 2202 |
| PceI | AGGCCT | 1 cut(s) | 1658 |
| PciI | ACATGT | 1 cut(s) | 1781 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 174 |
| PctI | GAATGC | 2 cut(s) | 1003, 1590 |
| PfeI | GAWTC | 1 cut(s) | 1770 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1188, 2174 |
| PkrI | GCNGC | 5 cut(s) | 313, 1049, 2041, 2076, 2097 |
| PleI | GAGTC | 4 cut(s) | 647, 1306, 1572, 1681 |
| PmaCI | CACGTG | 1 cut(s) | 2159 |
| PmlI | CACGTG | 1 cut(s) | 2159 |
| PpsI | GAGTC | 4 cut(s) | 647, 1306, 1572, 1681 |
| Ppu21I | YACGTR | 1 cut(s) | 2159 |
| PpuMI | RGGWCCY | 1 cut(s) | 619 |
| PscI | ACATGT | 1 cut(s) | 1781 |
| PshAI | GACNNNNGTC | 3 cut(s) | 617, 1113, 1329 |
| PsiI | TTATAA | 1 cut(s) | 1419 |
| Psp124BI | GAGCTC | 1 cut(s) | 44 |
| Psp5II | RGGWCCY | 1 cut(s) | 619 |
| Psp6I | CCWGG | 1 cut(s) | 504 |
| PspCI | CACGTG | 1 cut(s) | 2159 |
| PspFI | CCCAGC | 2 cut(s) | 254, 1885 |
| PspGI | CCWGG | 1 cut(s) | 504 |
| PspN4I | GGNNCC | 3 cut(s) | 405, 620, 792 |
| PspPI | GGNCC | 4 cut(s) | 431, 619, 1855, 1969 |
| PspPPI | RGGWCCY | 1 cut(s) | 619 |
| PspXI | VCTCGAGB | 1 cut(s) | 729 |
| PstI | CTGCAG | 1 cut(s) | 316 |
| PstNI | CAGNNNCTG | 1 cut(s) | 764 |
| PsuI | RGATCY | 3 cut(s) | 258, 790, 843 |
| PteI | GCGCGC | 1 cut(s) | 2202 |
| PvuII | CAGCTG | 2 cut(s) | 110, 1061 |
| RsaI | GTAC | 4 cut(s) | 248, 573, 757, 2140 |
| RsaNI | GTAC | 4 cut(s) | 247, 572, 756, 2139 |
| RseI | CAYNNNNRTG | 2 cut(s) | 281, 1061 |
| SacI | GAGCTC | 1 cut(s) | 44 |
| SaqAI | TTAA | 3 cut(s) | 479, 497, 1505 |
| SatI | GCNGC | 5 cut(s) | 312, 1048, 2040, 2075, 2096 |
| Sau96I | GGNCC | 4 cut(s) | 431, 619, 1855, 1969 |
| SchI | GAGTC | 4 cut(s) | 647, 1307, 1573, 1682 |
| ScrFI | CCNGG | 3 cut(s) | 182, 506, 2197 |
| SduI | GDGCHC | 5 cut(s) | 44, 406, 423, 1886, 2067 |
| SfaNI | GCATC | 2 cut(s) | 862, 1459 |
| SfcI | CTRYAG | 5 cut(s) | 111, 312, 1473, 1527, 1627 |
| Sfr274I | CTCGAG | 1 cut(s) | 729 |
| SfuI | TTCGAA | 1 cut(s) | 1926 |
| SinI | GGWCC | 3 cut(s) | 431, 619, 1969 |
| SlaI | CTCGAG | 1 cut(s) | 729 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 281, 1061 |
| SmlI | CTYRAG | 2 cut(s) | 729, 1151 |
| SmoI | CTYRAG | 2 cut(s) | 729, 1151 |
| SpeI | ACTAGT | 1 cut(s) | 1174 |
| SseBI | AGGCCT | 1 cut(s) | 1658 |
| SsiI | CCGC | 5 cut(s) | 45, 429, 553, 625, 1865 |
| SspMI | CTAG | 5 cut(s) | 491, 753, 1175, 1226, 2212 |
| SstI | GAGCTC | 1 cut(s) | 44 |
| StuI | AGGCCT | 1 cut(s) | 1658 |
| StyD4I | CCNGG | 3 cut(s) | 180, 504, 2195 |
| StyI | CCWWGG | 4 cut(s) | 10, 343, 424, 1302 |
| TaaI | ACNGT | 6 cut(s) | 69, 760, 781, 1477, 1918, 2069 |
| TaiI | ACGT | 3 cut(s) | 180, 892, 2161 |
| TaqI | TCGA | 7 cut(s) | 238, 730, 1243, 1323, 1434, 1676, 1926 |
| TatI | WGTACW | 3 cut(s) | 246, 755, 2138 |
| TfiI | GAWTC | 1 cut(s) | 1770 |
| Tru1I | TTAA | 3 cut(s) | 479, 497, 1505 |
| Tru9I | TTAA | 3 cut(s) | 479, 497, 1505 |
| TscAI | CASTG | 6 cut(s) | 256, 582, 786, 1482, 1923, 1965 |
| TseFI | GTSAC | 3 cut(s) | 274, 650, 1956 |
| TseI | GCWGC | 5 cut(s) | 311, 1047, 2039, 2074, 2095 |
| Tsp45I | GTSAC | 3 cut(s) | 274, 650, 1956 |
| TspDTI | ATGAA | 2 cut(s) | 483, 1454 |
| TspGWI | ACGGA | 2 cut(s) | 550, 1656 |
| TspRI | CASTG | 6 cut(s) | 256, 582, 786, 1482, 1923, 1965 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1188, 2174 |
| VneI | GTGCAC | 1 cut(s) | 419 |
| VpaK11BI | GGWCC | 3 cut(s) | 431, 619, 1969 |
| XceI | RCATGY | 1 cut(s) | 1785 |
| XhoI | CTCGAG | 1 cut(s) | 729 |
| XmiI | GTMKAC | 1 cut(s) | 1409 |
| XspI | CTAG | 5 cut(s) | 491, 753, 1175, 1226, 2212 |
| ZraI | GACGTC | 1 cut(s) | 178 |