FvH4_2g33060

A-kinase anchor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010497
Physical Location & Seq
Reverse (-)
24890219 .. 24892875
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33060.t2

Sequence Viewer

Length: 1005 bp
ATGAGCACGCTGAAGCTCGACTCGCTTTCTCCGGCTGAAACCGTGGAGATAGAGAACGGCCTATCCCTAGTGCCGCGGCTGAGGCTCAATATAACAGTGTATCCATCGAGCCAAGTTGTGAGCAAACCGATTGACGAGTGGAAGATGAAGCAAACCCTAATTGACTTCATGAAGAGCTCGCTTTCCGATCCGATTATGGTTCCGGAGGAGGATCTGAAGATCCGAAGGGTGAAGGATCTCAAGAAGCGGAAGCGGGACGACCCTGTGGCTTCTGGAACCATCTTCATTAGGGACCTAGGGTTTCTGAGGAAGAAGAGCAGGAAGGATGAGGATGAGGAGAGGAGCGTTGAGGAGGTGGAGAAGAAGTTTAGGGATTGGAGGAGGTACATTGTGGAGAAGATGGATGGGATTGAGCTTAATCTTGAAGGGGTCAAGTTCAAGCTAAACGTCGCCGTTCCGGAGTCGGATGATTTTAATGGGATGAAGAAGGGCTGGGAGGAGTTTCACGCTTTCGGAAATAGAGGGTTTTCGAAGAAGCAAGAGACTGATACTATAATTCTGAGGGGAGTTCCTTCACGGTGGTTCGCAGAGCCGCGGGTTTCGTCAAAGCCTTCAATGCTGGTTACACATACCATCTTTTCGACATTTGGGAAGATAAGGAATCTTAATGTTGCCGAGGACGATGATCCAGGTAAGGATGCGAATGAGGATGGTGGGGACTTGGTTTCAGGTCTCCATTGTAAGATTGTGGTTCAATTCGAGAAACACAAGGACTTCTACAATACTTTCAAATCGTTTTGTGGCCGCTCATTGCAGAAGGAAGGATCTAAGATGAAGGCTGATTATGAGGTTGCTTGGGACAAAGATGGATTCTTCCTAAACTTGAGAAATCAAACACAAGAAAACAGTAGTAGGGCACGAGAAACCACTACATATCCCAGGCAGCAGCAACACTTTTCTCGGTTTAGTGCCAATGAAGCTCCCAGAAAGAGGTTTAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

335

Amino Acids

38.75

Weight (kDa)

9.04

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_AKAP-17A PF25015 119 - 294 3.5e-31 AKAP-17A-like, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 807
AccII CGCG 2 cut(s) 76, 595
AccIII TCCGGA 2 cut(s) 202, 457
AciI CCGC 7 cut(s) 74, 76, 247, 253, 593, 595, 805
AclWI GGATC 6 cut(s) 182, 214, 219, 243, 680, 832
AcoI YGGCCR 1 cut(s) 802
AcuI CTGAAG 2 cut(s) 32, 236
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 1 cut(s) 990
AgsI TTSAA 5 cut(s) 425, 439, 615, 755, 790
AjnI CCWGG 2 cut(s) 688, 938
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 5 cut(s) 16, 177, 415, 442, 980
AluI AGCT 5 cut(s) 16, 177, 415, 442, 980
Alw21I GWGCWC 2 cut(s) 8, 179
Alw26I GTCTC 2 cut(s) 536, 737
AlwI GGATC 6 cut(s) 182, 214, 219, 243, 680, 832
Aor13HI TCCGGA 2 cut(s) 202, 457
AoxI GGCC 2 cut(s) 58, 802
ApeKI GCWGC 2 cut(s) 943, 946
AspA2I CCTAGG 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 530
AvaII GGWCC 1 cut(s) 292
AvrII CCTAGG 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 919
BanII GRGCYC 1 cut(s) 179
BauI CACGAG 1 cut(s) 918
Bbv12I GWGCWC 2 cut(s) 8, 179
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 955, 958
BccI CCATC 7 cut(s) 112, 287, 394, 398, 641, 704, 860
BceAI ACGGC 2 cut(s) 73, 437
BciT130I CCWGG 2 cut(s) 690, 940
BciVI GTATCC 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 536, 737
BfaI CTAG 2 cut(s) 68, 296
BfuI GTATCC 1 cut(s) 111
BisI GCNGC 6 cut(s) 74, 77, 593, 805, 944, 947
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 6 cut(s) 75, 78, 594, 806, 945, 948
Bme1390I CCNGG 2 cut(s) 690, 940
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 3 cut(s) 201, 277, 293
BmrFI CCNGG 2 cut(s) 690, 940
BmsI GCATC 1 cut(s) 688
Bpu10I CCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 224, 904
BsaI GGTCTC 1 cut(s) 737
BsaJI CCNNGG 6 cut(s) 42, 74, 295, 593, 675, 938
BsaWI WCCGGW 2 cut(s) 202, 457
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 990
Bse3DI GCAATG 1 cut(s) 809
BseAI TCCGGA 2 cut(s) 202, 457
BseBI CCWGG 2 cut(s) 690, 940
BseDI CCNNGG 6 cut(s) 42, 74, 295, 593, 675, 938
BseGI GGATG 7 cut(s) 331, 337, 409, 472, 486, 703, 715
BseLI CCNNNNNNNGG 1 cut(s) 990
BseMI GCAATG 1 cut(s) 809
BseMII CTCAG 3 cut(s) 71, 296, 551
BseRI GAGGAG 6 cut(s) 221, 350, 355, 365, 394, 512
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 2 cut(s) 955, 958
BseYI CCCAGC 1 cut(s) 492
Bsh1236I CGCG 2 cut(s) 76, 595
BshFI GGCC 2 cut(s) 60, 804
BsiHKAI GWGCWC 2 cut(s) 8, 179
BsiSI CCGG 3 cut(s) 32, 203, 458
BslFI GGGAC 4 cut(s) 269, 305, 731, 872
BslI CCNNNNNNNGG 1 cut(s) 990
BsmAI GTCTC 2 cut(s) 536, 737
BsmFI GGGAC 4 cut(s) 269, 305, 731, 872
BsnI GGCC 2 cut(s) 60, 804
Bso31I GGTCTC 1 cut(s) 737
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 3 cut(s) 8, 179, 919
Bsp13I TCCGGA 2 cut(s) 202, 457
Bsp143I GATC 6 cut(s) 187, 211, 219, 235, 685, 824
BspACI CCGC 7 cut(s) 74, 76, 247, 253, 593, 595, 805
BspANI GGCC 2 cut(s) 60, 804
BspCNI CTCAG 3 cut(s) 72, 297, 552
BspEI TCCGGA 2 cut(s) 202, 457
BspFNI CGCG 2 cut(s) 76, 595
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 3 cut(s) 201, 277, 293
BspPI GGATC 6 cut(s) 182, 214, 219, 243, 680, 832
BspQI GCTCTTC 2 cut(s) 167, 308
BspT104I TTCGAA 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 737
BsrBI CCGCTC 1 cut(s) 807
BsrDI GCAATG 1 cut(s) 809
BssECI CCNNGG 6 cut(s) 42, 74, 295, 593, 675, 938
BssMI GATC 6 cut(s) 187, 211, 219, 235, 685, 824
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 690, 940
Bst4CI ACNGT 4 cut(s) 43, 97, 579, 908
Bst6I CTCTTC 2 cut(s) 167, 308
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 8, 179
BstDEI CTNAG 4 cut(s) 80, 305, 560, 828
BstDSI CCRYGG 3 cut(s) 42, 74, 593
BstF5I GGATG 7 cut(s) 331, 337, 409, 472, 486, 703, 715
BstFNI CGCG 2 cut(s) 76, 595
BstKTI GATC 6 cut(s) 190, 214, 222, 238, 688, 827
BstMAI GTCTC 2 cut(s) 536, 737
BstMBI GATC 6 cut(s) 187, 211, 219, 235, 685, 824
BstMWI GCNNNNNNNGC 4 cut(s) 22, 82, 616, 977
BstNI CCWGG 2 cut(s) 690, 940
BstSCI CCNGG 2 cut(s) 688, 938
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 2 cut(s) 76, 595
BstV1I GCAGC 2 cut(s) 955, 958
BstX2I RGATCY 4 cut(s) 211, 219, 235, 824
BstYI RGATCY 4 cut(s) 211, 219, 235, 824
BsuI GTATCC 1 cut(s) 111
BsuRI GGCC 2 cut(s) 60, 804
BtgI CCRYGG 3 cut(s) 42, 74, 593
BtsCI GGATG 7 cut(s) 331, 337, 409, 472, 486, 703, 715
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 2 cut(s) 8, 179
CciI TCATGA 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 292
Cfr42I CCGCGG 2 cut(s) 77, 596
Csp6I GTAC 1 cut(s) 385
CviAII CATG 1 cut(s) 169
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 4 cut(s) 80, 305, 560, 828
DpnI GATC 6 cut(s) 189, 213, 221, 237, 687, 826
DpnII GATC 6 cut(s) 187, 211, 219, 235, 685, 824
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 2 cut(s) 167, 308
EarI CTCTTC 2 cut(s) 167, 308
Ecl136II GAGCTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 179
Eco31I GGTCTC 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 32, 236
EcoICRI GAGCTC 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 688, 938
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 172
FaiI YATR 7 cut(s) 92, 170, 197, 554, 630, 846, 934
FaqI GGGAC 4 cut(s) 269, 305, 731, 872
FatI CATG 1 cut(s) 168
FauI CCCGC 2 cut(s) 246, 588
Fnu4HI GCNGC 6 cut(s) 74, 77, 593, 805, 944, 947
FokI GGATG 7 cut(s) 338, 344, 416, 479, 493, 710, 722
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 6 cut(s) 74, 77, 593, 805, 944, 947
FspBI CTAG 2 cut(s) 68, 296
GluI GCNGC 6 cut(s) 74, 77, 593, 805, 944, 947
GsaI CCCAGC 1 cut(s) 496
HaeIII GGCC 2 cut(s) 60, 804
HapII CCGG 3 cut(s) 32, 203, 458
Hin1II CATG 1 cut(s) 172
HinfI GANTC 4 cut(s) 20, 461, 661, 870
HpaII CCGG 3 cut(s) 32, 203, 458
HphI GGTGA 1 cut(s) 241
Hpy188I TCNGA 8 cut(s) 187, 192, 216, 224, 306, 466, 515, 561
Hpy188III TCNNGA 7 cut(s) 169, 203, 241, 273, 422, 458, 760
Hpy99I CGWCG 1 cut(s) 452
HpyCH4III ACNGT 4 cut(s) 43, 97, 579, 908
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 1 cut(s) 814
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 82, 616, 977
HpyF3I CTNAG 4 cut(s) 80, 305, 560, 828
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 1 cut(s) 172
Kpn2I TCCGGA 2 cut(s) 202, 457
KspI CCGCGG 2 cut(s) 77, 596
Kzo9I GATC 6 cut(s) 187, 211, 219, 235, 685, 824
LguI GCTCTTC 2 cut(s) 167, 308
LmnI GCTCC 2 cut(s) 342, 985
Lsp1109I GCAGC 2 cut(s) 955, 958
LweI GCATC 1 cut(s) 688
MaeI CTAG 2 cut(s) 68, 296
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 622
MalI GATC 6 cut(s) 189, 213, 221, 237, 687, 826
MbiI CCGCTC 1 cut(s) 807
MboI GATC 6 cut(s) 187, 211, 219, 235, 685, 824
MflI RGATCY 4 cut(s) 211, 219, 235, 824
MhlI GDGCHC 3 cut(s) 8, 179, 919
MluCI AATT 3 cut(s) 159, 555, 755
MlyI GAGTC 2 cut(s) 14, 470
MmeI TCCRAC 1 cut(s) 444
MroI TCCGGA 2 cut(s) 202, 457
MseI TTAA 4 cut(s) 417, 474, 666, 996
MspA1I CMGCKG 2 cut(s) 76, 595
MspI CCGG 3 cut(s) 32, 203, 458
MspR9I CCNGG 2 cut(s) 690, 940
MvaI CCWGG 2 cut(s) 690, 940
MvnI CGCG 2 cut(s) 76, 595
MwoI GCNNNNNNNGC 4 cut(s) 22, 82, 616, 977
NdeII GATC 6 cut(s) 187, 211, 219, 235, 685, 824
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 201, 277, 293
NmeAIII GCCGAG 1 cut(s) 700
NspV TTCGAA 1 cut(s) 530
PagI TCATGA 1 cut(s) 168
PciSI GCTCTTC 2 cut(s) 167, 308
PfeI GAWTC 2 cut(s) 661, 870
PkrI GCNGC 6 cut(s) 75, 78, 594, 806, 945, 948
PleI GAGTC 2 cut(s) 14, 469
PpsI GAGTC 2 cut(s) 14, 469
PpuMI RGGWCCY 1 cut(s) 292
Psp124BI GAGCTC 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 688, 938
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 2 cut(s) 688, 938
PspN4I GGNNCC 3 cut(s) 201, 277, 293
PspPI GGNCC 1 cut(s) 292
PspPPI RGGWCCY 1 cut(s) 292
PsuI RGATCY 4 cut(s) 211, 219, 235, 824
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacI GAGCTC 1 cut(s) 179
SacII CCGCGG 2 cut(s) 77, 596
SapI GCTCTTC 2 cut(s) 167, 308
SaqAI TTAA 4 cut(s) 417, 474, 666, 996
SatI GCNGC 6 cut(s) 74, 77, 593, 805, 944, 947
Sau3AI GATC 6 cut(s) 187, 211, 219, 235, 685, 824
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 2 cut(s) 14, 470
ScrFI CCNGG 2 cut(s) 690, 940
SduI GDGCHC 3 cut(s) 8, 179, 919
SfaNI GCATC 1 cut(s) 688
Sfr303I CCGCGG 2 cut(s) 77, 596
SfuI TTCGAA 1 cut(s) 530
SgrBI CCGCGG 2 cut(s) 77, 596
SinI GGWCC 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 239, 883
SmoI CTYRAG 2 cut(s) 239, 883
Sse9I AATT 3 cut(s) 159, 555, 755
SsiI CCGC 7 cut(s) 74, 76, 247, 253, 593, 595, 805
SspMI CTAG 2 cut(s) 68, 296
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 688, 938
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 43, 97, 579, 908
TaiI ACGT 1 cut(s) 450
TaqI TCGA 5 cut(s) 18, 107, 530, 641, 759
TasI AATT 3 cut(s) 159, 555, 755
TauI GCSGC 4 cut(s) 76, 79, 595, 807
TfiI GAWTC 2 cut(s) 661, 870
Tru1I TTAA 4 cut(s) 417, 474, 666, 996
Tru9I TTAA 4 cut(s) 417, 474, 666, 996
TscAI CASTG 1 cut(s) 102
TseI GCWGC 2 cut(s) 943, 946
TspDTI ATGAA 7 cut(s) 157, 161, 185, 274, 497, 848, 990
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 295
XspI CTAG 2 cut(s) 68, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.