Rroxscaffold_7G00159250

A-kinase anchor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010497
Physical Location & Seq
Reverse (-)
2494568 .. 2496473
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159250.1

Sequence Viewer

Length: 1017 bp
ATGAGCACGCTCAACCTGGATTCGATTTCTCCTACTGAAACTGTGGAGATAGAGAATGGTCTGTCACTGGTGCCGCGGCTGCGGCTGAATATAACGGTGTATCCGTCGAGCCAAGTTGTGAGCAAACCGATTGACGAGTGGAAGATGAAGCAAACCCTAATTGACTTCCTCAAGAGCTCACTTTCCGATCCGGTCACAGTTCCGGAGGAGGATCTCAAGATCAGACGGGTGAGGGACCTGAAGAAGCGGAAGCGGGACGACCCTGTAGCTTCCGGTACCATCTTCATTCGCGACCTAGGGTTTCTGAGGAAGACGAGTAGGAAGGGTGAGGAGGATGAGGAGAAGAAGTTCAGTGATTGGAGGAGGTACATTGTGGAGAAGATGGATGGGATTGAGCTCAATCTCGAAGGGGTTAAGTTCAAGCTAAACGTCGCCGTTCCGGAGTCGGATGATTTCAAGGCGATGAAGAAAGGTTGGGAGGAGTTTTACGCTCTCGGAAATCGAGGGTTTTCGAGGAGTGGGAAGCAAGAGACTGATACTATAATACTGAGGGGTGTTCCTTCACGCTGGTTCGCAGAGCCGCGGGTTTCCTCAAAGCCTTCAATGCTGGTTACACATACCATCTTTTCAACATTTGGGAAGATAAGGCATGTCAATGTGTTTGACGATGATCCAGGTAAGGATGCGAATGAGGATGATGGGGACTTGGTTTCAGGTCTCCATTGTAAGATTGTGGTTCAGTTCGAGAAACACAAGGACTTCTACAATACTTTCAAGGCGTTTTGTGGCCGCTCATTGCAAAAGGAAGGATCTAAGTTGAAGGCTGATTATGAGGTTGCTTGGGACAAGGATGGATTTTTCCGAAACTCGAGAAATCAAACACAGGAAGACAGTAGCAGGGCACGAGAAACCACAACATATCCCAGGCTGCAGCAACACTATTCTCGGTTCAGTTCCGATGAAGCTCCCAGAAAGAGGTTTAAGGTTAGAAACTCAATAGCTAACTACTCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

39.07

Weight (kDa)

9.14

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_AKAP-17A PF25015 115 - 293 3.2e-32 AKAP-17A-like, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 70, 275
AccBSI CCGCTC 1 cut(s) 792
AccII CGCG 3 cut(s) 76, 291, 583
AccIII TCCGGA 2 cut(s) 202, 439
AciI CCGC 8 cut(s) 74, 76, 82, 247, 253, 581, 583, 790
AclWI GGATC 4 cut(s) 182, 219, 665, 817
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 2 cut(s) 277, 368
AfiI CCNNNNNNNGG 1 cut(s) 975
AgsI TTSAA 6 cut(s) 421, 457, 603, 630, 775, 820
AjnI CCWGG 3 cut(s) 15, 673, 923
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 6 cut(s) 177, 269, 397, 424, 965, 1001
AluI AGCT 6 cut(s) 177, 269, 397, 424, 965, 1001
Alw21I GWGCWC 3 cut(s) 8, 179, 399
Alw26I GTCTC 2 cut(s) 524, 722
AlwI GGATC 4 cut(s) 182, 219, 665, 817
Ama87I CYCGRG 1 cut(s) 868
Aor13HI TCCGGA 2 cut(s) 202, 439
AoxI GGCC 1 cut(s) 787
ApeKI GCWGC 3 cut(s) 79, 928, 931
Asp700I GAANNNNTTC 1 cut(s) 347
Asp718I GGTACC 1 cut(s) 275
AspA2I CCTAGG 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 241, 338
AvaI CYCGRG 1 cut(s) 868
AvaII GGWCC 1 cut(s) 235
AvrII CCTAGG 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 904
BanI GGYRCC 2 cut(s) 70, 275
BanII GRGCYC 2 cut(s) 179, 399
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 2 cut(s) 317, 894
Bbv12I GWGCWC 3 cut(s) 8, 179, 399
BbvI GCAGC 3 cut(s) 66, 915, 943
BccI CCATC 6 cut(s) 287, 376, 380, 629, 692, 845
BceAI ACGGC 1 cut(s) 419
BciT130I CCWGG 3 cut(s) 17, 675, 925
BciVI GTATCC 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 524, 722
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 264, 929
BfuI GTATCC 1 cut(s) 111
BisI GCNGC 8 cut(s) 74, 77, 80, 83, 581, 790, 929, 932
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 8 cut(s) 75, 78, 81, 84, 582, 791, 930, 933
Bme1390I CCNGG 3 cut(s) 17, 675, 925
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 3 cut(s) 72, 236, 277
BmrFI CCNGG 3 cut(s) 17, 675, 925
BmsI GCATC 1 cut(s) 673
BpiI GAAGAC 2 cut(s) 317, 894
BpuEI CTTGAG 2 cut(s) 155, 200
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 4 cut(s) 74, 295, 581, 923
BsaWI WCCGGW 4 cut(s) 190, 202, 272, 439
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 975
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 794
BseAI TCCGGA 2 cut(s) 202, 439
BseBI CCWGG 3 cut(s) 17, 675, 925
BseDI CCNNGG 4 cut(s) 74, 295, 581, 923
BseGI GGATG 6 cut(s) 340, 391, 454, 688, 700, 856
BseLI CCNNNNNNNGG 1 cut(s) 975
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 2 cut(s) 296, 539
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 6 cut(s) 221, 344, 353, 376, 494, 529
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 3 cut(s) 66, 915, 943
Bsh1236I CGCG 3 cut(s) 76, 291, 583
BshFI GGCC 1 cut(s) 789
BshNI GGYRCC 2 cut(s) 70, 275
BsiHKAI GWGCWC 3 cut(s) 8, 179, 399
BsiHKCI CYCGRG 1 cut(s) 868
BsiSI CCGG 4 cut(s) 191, 203, 273, 440
BslFI GGGAC 4 cut(s) 248, 269, 716, 857
BslI CCNNNNNNNGG 1 cut(s) 975
BsmAI GTCTC 2 cut(s) 524, 722
BsmFI GGGAC 4 cut(s) 248, 269, 716, 857
BsnI GGCC 1 cut(s) 789
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 868
Bsp1286I GDGCHC 4 cut(s) 8, 179, 399, 904
Bsp13I TCCGGA 2 cut(s) 202, 439
Bsp143I GATC 5 cut(s) 187, 211, 219, 670, 809
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 8 cut(s) 74, 76, 82, 247, 253, 581, 583, 790
BspANI GGCC 1 cut(s) 789
BspCNI CTCAG 2 cut(s) 297, 540
BspEI TCCGGA 2 cut(s) 202, 439
BspFNI CGCG 3 cut(s) 76, 291, 583
BspLI GGNNCC 3 cut(s) 72, 236, 277
BspMAI CTGCAG 1 cut(s) 933
BspPI GGATC 4 cut(s) 182, 219, 665, 817
BspT107I GGYRCC 2 cut(s) 70, 275
BspTNI GGTCTC 1 cut(s) 722
BsrBI CCGCTC 1 cut(s) 792
BsrDI GCAATG 1 cut(s) 794
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 4 cut(s) 74, 295, 581, 923
BssMI GATC 5 cut(s) 187, 211, 219, 670, 809
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 3 cut(s) 17, 675, 925
Bst4CI ACNGT 4 cut(s) 43, 97, 199, 893
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 3 cut(s) 305, 548, 813
BstDSI CCRYGG 2 cut(s) 74, 581
BstF5I GGATG 6 cut(s) 340, 391, 454, 688, 700, 856
BstFNI CGCG 3 cut(s) 76, 291, 583
BstKTI GATC 5 cut(s) 190, 214, 222, 673, 812
BstMAI GTCTC 2 cut(s) 524, 722
BstMBI GATC 5 cut(s) 187, 211, 219, 670, 809
BstMWI GCNNNNNNNGC 3 cut(s) 79, 82, 604
BstNI CCWGG 3 cut(s) 17, 675, 925
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 3 cut(s) 15, 673, 923
BstSFI CTRYAG 2 cut(s) 264, 929
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 3 cut(s) 76, 291, 583
BstV1I GCAGC 3 cut(s) 66, 915, 943
BstV2I GAAGAC 2 cut(s) 317, 894
BstX2I RGATCY 2 cut(s) 211, 809
BstYI RGATCY 2 cut(s) 211, 809
BsuI GTATCC 1 cut(s) 111
BsuRI GGCC 1 cut(s) 789
BtgI CCRYGG 2 cut(s) 74, 581
BtgZI GCGATG 1 cut(s) 476
BtsCI GGATG 6 cut(s) 340, 391, 454, 688, 700, 856
BtsIMutI CAGTG 2 cut(s) 65, 358
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 2 cut(s) 77, 584
Csp6I GTAC 2 cut(s) 276, 367
CviAII CATG 1 cut(s) 650
CviQI GTAC 2 cut(s) 276, 367
DdeI CTNAG 3 cut(s) 305, 548, 813
DpnI GATC 5 cut(s) 189, 213, 221, 672, 811
DpnII GATC 5 cut(s) 187, 211, 219, 670, 809
EaeI YGGCCR 1 cut(s) 787
Ecl136II GAGCTC 2 cut(s) 177, 397
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 179, 399
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 2 cut(s) 177, 397
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 868
EcoICRI GAGCTC 2 cut(s) 177, 397
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 15, 673, 923
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 2 cut(s) 179, 399
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 653
FaiI YATR 6 cut(s) 92, 542, 618, 651, 831, 919
FaqI GGGAC 4 cut(s) 248, 269, 716, 857
FatI CATG 1 cut(s) 649
FauI CCCGC 2 cut(s) 246, 576
Fnu4HI GCNGC 8 cut(s) 74, 77, 80, 83, 581, 790, 929, 932
FokI GGATG 6 cut(s) 347, 398, 461, 695, 707, 863
FriOI GRGCYC 2 cut(s) 179, 399
Fsp4HI GCNGC 8 cut(s) 74, 77, 80, 83, 581, 790, 929, 932
FspBI CTAG 1 cut(s) 296
GluI GCNGC 8 cut(s) 74, 77, 80, 83, 581, 790, 929, 932
HaeIII GGCC 1 cut(s) 789
HapII CCGG 4 cut(s) 191, 203, 273, 440
Hin1II CATG 1 cut(s) 653
HinfI GANTC 2 cut(s) 20, 443
HpaII CCGG 4 cut(s) 191, 203, 273, 440
HphI GGTGA 2 cut(s) 241, 338
Hpy188I TCNGA 7 cut(s) 187, 224, 306, 448, 497, 863, 958
Hpy188III TCNNGA 8 cut(s) 172, 203, 217, 290, 404, 440, 745, 870
Hpy99I CGWCG 2 cut(s) 109, 434
HpyAV CCTTC 6 cut(s) 316, 401, 570, 609, 800, 814
HpyCH4III ACNGT 4 cut(s) 43, 97, 199, 893
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 2 cut(s) 799, 931
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 82, 604
HpyF3I CTNAG 3 cut(s) 305, 548, 813
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 1 cut(s) 653
Kpn2I TCCGGA 2 cut(s) 202, 439
KpnI GGTACC 1 cut(s) 279
KspI CCGCGG 2 cut(s) 77, 584
Kzo9I GATC 5 cut(s) 187, 211, 219, 670, 809
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 3 cut(s) 66, 915, 943
LweI GCATC 1 cut(s) 673
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 429
MaeIII GTNAC 3 cut(s) 63, 193, 610
MalI GATC 5 cut(s) 189, 213, 221, 672, 811
MbiI CCGCTC 1 cut(s) 792
MboI GATC 5 cut(s) 187, 211, 219, 670, 809
MboII GAAGA 9 cut(s) 154, 253, 274, 322, 355, 391, 478, 652, 899
MflI RGATCY 2 cut(s) 211, 809
MhlI GDGCHC 4 cut(s) 8, 179, 399, 904
MluCI AATT 2 cut(s) 159, 1011
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 426
MroI TCCGGA 2 cut(s) 202, 439
MroXI GAANNNNTTC 1 cut(s) 347
MseI TTAA 2 cut(s) 414, 981
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 2 cut(s) 76, 583
MspI CCGG 4 cut(s) 191, 203, 273, 440
MspR9I CCNGG 3 cut(s) 17, 675, 925
MvaI CCWGG 3 cut(s) 17, 675, 925
MvnI CGCG 3 cut(s) 76, 291, 583
MwoI GCNNNNNNNGC 3 cut(s) 79, 82, 604
NdeII GATC 5 cut(s) 187, 211, 219, 670, 809
NlaIII CATG 1 cut(s) 653
NlaIV GGNNCC 3 cut(s) 72, 236, 277
NmuCI GTSAC 2 cut(s) 63, 193
NruI TCGCGA 1 cut(s) 291
NspI RCATGY 1 cut(s) 653
PaeR7I CTCGAG 1 cut(s) 868
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 8 cut(s) 75, 78, 81, 84, 582, 791, 930, 933
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 2 cut(s) 179, 399
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 15, 673, 923
PspGI CCWGG 3 cut(s) 15, 673, 923
PspN4I GGNNCC 3 cut(s) 72, 236, 277
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 1 cut(s) 933
PsuI RGATCY 2 cut(s) 211, 809
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 277, 368
RsaNI GTAC 2 cut(s) 276, 367
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 2 cut(s) 179, 399
SacII CCGCGG 2 cut(s) 77, 584
SaqAI TTAA 2 cut(s) 414, 981
SatI GCNGC 8 cut(s) 74, 77, 80, 83, 581, 790, 929, 932
Sau3AI GATC 5 cut(s) 187, 211, 219, 670, 809
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 17, 675, 925
SduI GDGCHC 4 cut(s) 8, 179, 399, 904
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 2 cut(s) 264, 929
Sfr274I CTCGAG 1 cut(s) 868
Sfr303I CCGCGG 2 cut(s) 77, 584
SgrBI CCGCGG 2 cut(s) 77, 584
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 868
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 3 cut(s) 170, 215, 868
SmoI CTYRAG 3 cut(s) 170, 215, 868
Sse9I AATT 2 cut(s) 159, 1011
SsiI CCGC 8 cut(s) 74, 76, 82, 247, 253, 581, 583, 790
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 179, 399
StyD4I CCNGG 3 cut(s) 15, 673, 923
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 43, 97, 199, 893
TaiI ACGT 1 cut(s) 432
TaqI TCGA 7 cut(s) 23, 107, 405, 502, 512, 744, 869
TasI AATT 2 cut(s) 159, 1011
TauI GCSGC 5 cut(s) 76, 79, 85, 583, 792
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 414, 981
Tru9I TTAA 2 cut(s) 414, 981
TscAI CASTG 2 cut(s) 72, 358
TseFI GTSAC 2 cut(s) 63, 193
TseI GCWGC 3 cut(s) 79, 928, 931
Tsp45I GTSAC 2 cut(s) 63, 193
TspDTI ATGAA 4 cut(s) 161, 274, 479, 975
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 72, 358
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 1 cut(s) 653
XhoI CTCGAG 1 cut(s) 868
XmaJI CCTAGG 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.