Prupe.1G422600_v2.0.a1

A-kinase anchor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010497
Physical Location & Seq
Reverse (-)
36476789 .. 36479328
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G422600.2

Sequence Viewer

Length: 1035 bp
ATGACGAACAAACCGTTGGATTCACTACCTCCTACTGAAACCCTAGAGATGGAGAATGGATTGTCGCTGGTGCCACGCGTTAGGCTCAATCTCACAGTGTACTCGTCGAGCCAAGTTGTGACCAAACCCATCGATGAGTGGAAGATGAAGCAGACGCTCATAGACTTCCTAAAGAACTCGCTTTCTGTCTCCATCACCGTCCCTGAAGATGACCTCCAGATCAGACGACTCAAGGACCTCAAGAAGCGCAAGCGCGACGACCCTATAGCTTTCGGTACACTGTTCATTCGCAACCTGGGGTTTCTGAACAAGACCAGTAAGAGAAACGATGGTGAGGAAGAAGAAAAGGACGTAAAGGAGTTGGAGAAGAAGTTCCTGGACTGGAGAAAGTACATTGTTGAGAAAATGGATGGGATTGAGCTCAATATCGAAGGGGTCAAGTACAAGCTAAACGTCGACGTTCCGGAGTCTGATAATTTTGAGGCCATGAAGAAAGCTTGGGAGGAGTTTTACGCTTTTGGAAGTCGGGGGCTTTCGACGAGAGGGAGGCAAGAGCCTGATACCATAATACTGAGGGGTGCTCCGTCACGCTGGTTTGCAGAGCCCCGGGTTTCTTCGAAGCCTTCAATGCTGGTTACTCATACCATCTTTTCTACATTTGGGAAGATAAGGAATCTTAATGTTGCTGAGGACGATGGTCTAGGTAAGGACGCGAACGAAGATGGTGGGGACTTAATTTCAGGTCTCCATTGTAAGATTGTGGTTCAGTTCGAGAAATACCAGGACTTCTATAATACTTTCAAGGTGTTATGTGGCCGCTCATTCCAGAAGCAAGGATCTCAATTGAGGGCTGATTATGAGGTTACGTGGGACAAGGATGGATTTTTCCGATTCTCAAGAAATCAACCGCATGAAAATAGTAGCAGGATACGGGAAACAGCAACAACACAGCACAGAAGTGAAGCTCCAAGGCATCGGCAACGCATCTCTCAATTCAGTTCCAACCAAGCTCCCCGCAAGAGGTTTAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

345

Amino Acids

39.93

Weight (kDa)

9.16

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AccBSI CCGCTC 1 cut(s) 819
AccI GTMKAC 1 cut(s) 456
AccII CGCG 3 cut(s) 78, 255, 713
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 3 cut(s) 817, 908, 1015
AclWI GGATC 1 cut(s) 844
AcoI YGGCCR 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 4 cut(s) 101, 277, 392, 443
AfiI CCNNNNNNNGG 2 cut(s) 49, 1020
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 2 cut(s) 627, 802
AjnI CCWGG 3 cut(s) 294, 375, 780
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AleI CACNNNNGTG 1 cut(s) 957
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
AluBI AGCT 6 cut(s) 269, 421, 448, 497, 965, 1010
AluI AGCT 6 cut(s) 269, 421, 448, 497, 965, 1010
Alw21I GWGCWC 2 cut(s) 423, 583
Alw26I GTCTC 2 cut(s) 193, 749
AlwI GGATC 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 463
AoxI GGCC 2 cut(s) 483, 814
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 2 cut(s) 249, 255
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 607, 608
AsuHPI GGTGA 2 cut(s) 187, 344
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 2 cut(s) 423, 606
Bbv12I GWGCWC 2 cut(s) 423, 583
BbvCI CCTCAGC 1 cut(s) 687
BccI CCATC 9 cut(s) 43, 137, 200, 323, 404, 653, 689, 716, 872
BciT130I CCWGG 3 cut(s) 296, 377, 782
BciVI GTATCC 1 cut(s) 921
BcnI CCSGG 2 cut(s) 607, 608
BcoDI GTCTC 2 cut(s) 193, 749
BfaI CTAG 2 cut(s) 44, 701
BfmI CTRYAG 1 cut(s) 264
BfuI GTATCC 1 cut(s) 921
BisI GCNGC 1 cut(s) 817
BlsI GCNGC 1 cut(s) 818
Bme1390I CCNGG 5 cut(s) 296, 377, 607, 608, 782
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 5 cut(s) 296, 377, 607, 608, 782
BmsI GCATC 2 cut(s) 982, 993
BoxI GACNNNNGTC 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 2 cut(s) 200, 403
Bpu10I CCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 3 cut(s) 215, 224, 880
BpuMI CCSGG 2 cut(s) 607, 608
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 749
BsaJI CCNNGG 4 cut(s) 295, 605, 606, 968
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 1020
Bse1I ACTGG 2 cut(s) 315, 386
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 3 cut(s) 296, 377, 782
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 4 cut(s) 295, 605, 606, 968
BseGI GGATG 2 cut(s) 415, 883
BseLI CCNNNNNNNGG 2 cut(s) 49, 1020
BseMII CTCAG 2 cut(s) 563, 678
BseNI ACTGG 2 cut(s) 315, 386
BseRI GAGGAG 1 cut(s) 518
Bsh1236I CGCG 3 cut(s) 78, 255, 713
BshFI GGCC 2 cut(s) 485, 816
BshNI GGYRCC 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 423, 583
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 2 cut(s) 464, 607
BslFI GGGAC 3 cut(s) 185, 743, 884
BslI CCNNNNNNNGG 2 cut(s) 49, 1020
BsmAI GTCTC 2 cut(s) 193, 749
BsmFI GGGAC 3 cut(s) 185, 743, 884
BsnI GGCC 2 cut(s) 485, 816
Bso31I GGTCTC 1 cut(s) 749
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 423, 583, 606
Bsp13I TCCGGA 1 cut(s) 463
Bsp143I GATC 2 cut(s) 219, 836
BspACI CCGC 3 cut(s) 817, 908, 1015
BspANI GGCC 2 cut(s) 485, 816
BspCNI CTCAG 2 cut(s) 564, 679
BspDI ATCGAT 1 cut(s) 132
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 3 cut(s) 78, 255, 713
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 1 cut(s) 844
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 749
BsrBI CCGCTC 1 cut(s) 819
BsrI ACTGG 2 cut(s) 315, 386
BssECI CCNNGG 4 cut(s) 295, 605, 606, 968
BssMI GATC 2 cut(s) 219, 836
BssT1I CCWWGG 1 cut(s) 968
Bst2UI CCWGG 3 cut(s) 296, 377, 782
Bst4CI ACNGT 4 cut(s) 15, 97, 199, 282
BstBAI YACGTR 1 cut(s) 867
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 2 cut(s) 572, 687
BstF5I GGATG 2 cut(s) 415, 883
BstFNI CGCG 3 cut(s) 78, 255, 713
BstHHI GCGC 2 cut(s) 249, 255
BstKTI GATC 2 cut(s) 222, 839
BstMAI GTCTC 2 cut(s) 193, 749
BstMBI GATC 2 cut(s) 219, 836
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 3 cut(s) 296, 377, 782
BstPAI GACNNNNGTC 1 cut(s) 696
BstSCI CCNGG 5 cut(s) 294, 375, 605, 606, 780
BstSFI CTRYAG 1 cut(s) 264
BstUI CGCG 3 cut(s) 78, 255, 713
BstX2I RGATCY 1 cut(s) 836
BstYI RGATCY 1 cut(s) 836
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 1 cut(s) 921
BsuRI GGCC 2 cut(s) 485, 816
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 2 cut(s) 415, 883
BtsIMutI CAGTG 2 cut(s) 102, 278
Cac8I GCNNGC 1 cut(s) 251
CfoI GCGC 2 cut(s) 249, 255
Cfr13I GGNCC 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 2 cut(s) 163, 719
Csp6I GTAC 4 cut(s) 100, 276, 391, 442
CviAII CATG 2 cut(s) 487, 911
CviQI GTAC 4 cut(s) 100, 276, 391, 442
DdeI CTNAG 2 cut(s) 572, 687
DpnI GATC 2 cut(s) 221, 838
DpnII GATC 2 cut(s) 219, 836
EaeI YGGCCR 1 cut(s) 814
Ecl136II GAGCTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 968
Eco24I GRGCYC 2 cut(s) 423, 606
Eco31I GGTCTC 1 cut(s) 749
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 294, 375, 780
EcoT14I CCWWGG 1 cut(s) 968
EcoT38I GRGCYC 2 cut(s) 423, 606
ErhI CCWWGG 1 cut(s) 968
FaeI CATG 2 cut(s) 490, 914
FaiI YATR 9 cut(s) 161, 266, 488, 566, 642, 792, 811, 858, 912
FaqI GGGAC 3 cut(s) 185, 743, 884
FatI CATG 2 cut(s) 486, 910
FauI CCCGC 1 cut(s) 1022
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 1 cut(s) 817
FokI GGATG 2 cut(s) 422, 890
FriOI GRGCYC 2 cut(s) 423, 606
Fsp4HI GCNGC 1 cut(s) 817
FspBI CTAG 2 cut(s) 44, 701
GlaI GCGC 2 cut(s) 248, 254
GluI GCNGC 1 cut(s) 817
GsuI CTGGAG 2 cut(s) 200, 403
HaeIII GGCC 2 cut(s) 485, 816
HapII CCGG 2 cut(s) 464, 607
HgaI GACGC 2 cut(s) 163, 719
HhaI GCGC 2 cut(s) 249, 255
Hin1II CATG 2 cut(s) 490, 914
Hin6I GCGC 2 cut(s) 247, 253
HinP1I GCGC 2 cut(s) 247, 253
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 5 cut(s) 20, 228, 467, 673, 891
HpaII CCGG 2 cut(s) 464, 607
HphI GGTGA 2 cut(s) 187, 344
Hpy166II GTNNAC 3 cut(s) 100, 278, 457
Hpy188I TCNGA 4 cut(s) 224, 306, 472, 890
Hpy188III TCNNGA 6 cut(s) 217, 241, 464, 772, 826, 897
Hpy8I GTNNAC 3 cut(s) 100, 278, 457
Hpy99I CGWCG 5 cut(s) 109, 260, 458, 461, 541
HpyAV CCTTC 2 cut(s) 425, 633
HpyCH4III ACNGT 4 cut(s) 15, 97, 199, 282
HpyCH4IV ACGT 4 cut(s) 351, 453, 459, 866
HpyCH4V TGCA 1 cut(s) 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 2 cut(s) 572, 687
HpySE526I ACGT 4 cut(s) 351, 453, 459, 866
Hsp92II CATG 2 cut(s) 490, 914
HspAI GCGC 2 cut(s) 247, 253
Kpn2I TCCGGA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 219, 836
LmnI GCTCC 3 cut(s) 586, 970, 1015
LweI GCATC 2 cut(s) 982, 993
MaeI CTAG 2 cut(s) 44, 701
MaeII ACGT 4 cut(s) 351, 453, 459, 866
MaeIII GTNAC 4 cut(s) 118, 585, 634, 862
MalI GATC 2 cut(s) 221, 838
MbiI CCGCTC 1 cut(s) 819
MboI GATC 2 cut(s) 219, 836
MboII GAAGA 9 cut(s) 154, 218, 350, 353, 379, 502, 606, 676, 731
MfeI CAATTG 1 cut(s) 842
MflI RGATCY 1 cut(s) 836
MhlI GDGCHC 3 cut(s) 423, 583, 606
MluCI AATT 4 cut(s) 475, 735, 842, 992
MluI ACGCGT 1 cut(s) 76
MlyI GAGTC 2 cut(s) 222, 476
MmeI TCCRAC 2 cut(s) 342, 1026
MroI TCCGGA 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 371
MseI TTAA 3 cut(s) 678, 734, 1026
MslI CAYNNNNRTG 1 cut(s) 957
MspI CCGG 2 cut(s) 464, 607
MspR9I CCNGG 5 cut(s) 296, 377, 607, 608, 782
MunI CAATTG 1 cut(s) 842
MvaI CCWGG 3 cut(s) 296, 377, 782
MvnI CGCG 3 cut(s) 78, 255, 713
MwoI GCNNNNNNNGC 1 cut(s) 628
NciI CCSGG 2 cut(s) 607, 608
NdeII GATC 2 cut(s) 219, 836
NlaIII CATG 2 cut(s) 490, 914
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 2 cut(s) 118, 585
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 3 cut(s) 20, 673, 891
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 818
PleI GAGTC 2 cut(s) 222, 475
PpsI GAGTC 2 cut(s) 222, 475
Ppu21I YACGTR 1 cut(s) 867
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 423
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 294, 375, 780
PspGI CCWGG 3 cut(s) 294, 375, 780
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 1 cut(s) 836
RsaI GTAC 4 cut(s) 101, 277, 392, 443
RsaNI GTAC 4 cut(s) 100, 276, 391, 442
RseI CAYNNNNRTG 1 cut(s) 957
SacI GAGCTC 1 cut(s) 423
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 3 cut(s) 678, 734, 1026
SatI GCNGC 1 cut(s) 817
Sau3AI GATC 2 cut(s) 219, 836
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 222, 476
ScrFI CCNGG 5 cut(s) 296, 377, 607, 608, 782
SduI GDGCHC 3 cut(s) 423, 583, 606
SfaNI GCATC 2 cut(s) 982, 993
SfcI CTRYAG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 617
SgrDI CGTCGACG 1 cut(s) 455
SinI GGWCC 1 cut(s) 235
SmaI CCCGGG 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 3 cut(s) 230, 239, 895
SmoI CTYRAG 3 cut(s) 230, 239, 895
Sse9I AATT 4 cut(s) 475, 735, 842, 992
SsiI CCGC 3 cut(s) 817, 908, 1015
SspMI CTAG 2 cut(s) 44, 701
SstI GAGCTC 1 cut(s) 423
StyD4I CCNGG 5 cut(s) 294, 375, 605, 606, 780
StyI CCWWGG 1 cut(s) 968
TaaI ACNGT 4 cut(s) 15, 97, 199, 282
TaiI ACGT 4 cut(s) 354, 456, 462, 869
TaqI TCGA 7 cut(s) 107, 132, 429, 456, 536, 617, 771
TasI AATT 4 cut(s) 475, 735, 842, 992
TatI WGTACW 3 cut(s) 99, 390, 441
TauI GCSGC 1 cut(s) 819
TfiI GAWTC 3 cut(s) 20, 673, 891
Tru1I TTAA 3 cut(s) 678, 734, 1026
Tru9I TTAA 3 cut(s) 678, 734, 1026
TscAI CASTG 2 cut(s) 102, 285
TseFI GTSAC 2 cut(s) 118, 585
Tsp45I GTSAC 2 cut(s) 118, 585
TspDTI ATGAA 4 cut(s) 161, 274, 503, 927
TspGWI ACGGA 1 cut(s) 573
TspMI CCCGGG 1 cut(s) 606
TspRI CASTG 2 cut(s) 102, 285
VpaK11BI GGWCC 1 cut(s) 235
XmaI CCCGGG 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 2 cut(s) 44, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.