MD15G1069100.v1.1

A-kinase anchor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010497
Physical Location & Seq
Reverse (-)
4761407 .. 4763536
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1069100.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGAGCGCCAAGCCGTTGGATTCGCTGCCTCCGACCGAAACCCTAGAGATAGAGAATGGGCTTTCGCTGGTGCCGCGCGTGAGGCTCAATCTCACGGTTTACTCGTCGACCGGAGTCGTGACGAAACCCGTCGACGAGTGGAAGATGAAGCGGACGCTCATCGACTTCCTCAAGAACTCGCTTTCCGTCCCCATCACCGTCCCCGAAGACGACCTCCAGATCCGGCGGCTCAGGGACCTCAAGAAGCGGAAGCGCGACGACCCCGTGGCTTTCGGCGTTCTCGTGATTCGCGACCTAGGGTTTCTGAAAAAGGGCGGGAAGCGAAGTGACGTGGAAGAAGAAGAGGATGCGAAGGAGTTGGAGAGGAAGTTTCTGGAATGGAGAAAGTACATTGTTGAGAAAATGGACGGGATTGAGCTCAACATCGAAGGGGTTAAGTACAAGCTGAATGTTGTTGTTCCAGATTCGGATGATTTCGAGGGCACCAAGAAGGCTTGGGAGGAGTTCTACGCCTTCGGAAATCGGGGGTTTTCGAGGGGGAAGCAAGAGCCGGATACGATAATAGTGAGGGGTGTTCCGTCACGCTGGTTCGCAGAGCCGCGGGTTTCTTCCTCCAAGTTTTCAGTGCTGGTTACCCATACCATATTTTCTACATTTGGGAAGATAAGGAATCTTAATGTTGCTGAGGACGATGGTCTAGGTAAGGATGCAAATGAGGATGGCGATGACTTAATTTCTGGTCTCCACTGTAAGATTGTGGTTCAGTTTGAGACATACCAGGACTTCTATAATGCCTTCAAGGTGTTATGCGGCCGCTCATTGCAGAAGGAAGGATCTAAATTGAGGGCTGATTATGAGGTTACATGGGACAAGGACGGATTCTTCCGATTCTCAAGAAATCAACCGGAGCAAAACAGTAGTAGGCTACGAGAAATGACGACGACAAATCAGAGAAGTGAAGCTCCCCGGCATCAGCAACGCGTTTCTCAATTCAGTGCGGACCAGGCTCCCCGAAAGAGGTTTAAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

39.54

Weight (kDa)

8.61

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_AKAP-17A PF25015 125 - 319 7.3e-33 AKAP-17A-like, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 70, 482
AccBSI CCGCTC 1 cut(s) 816
AccI GTMKAC 2 cut(s) 107, 132
AccII CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
AclWI GGATC 2 cut(s) 214, 841
AcoI YGGCCR 1 cut(s) 811
AfaI GTAC 2 cut(s) 389, 440
AfiI CCNNNNNNNGG 1 cut(s) 1017
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 1 cut(s) 799
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 2 cut(s) 777, 1002
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
AluBI AGCT 3 cut(s) 418, 445, 962
AluI AGCT 3 cut(s) 418, 445, 962
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 2 cut(s) 746, 764
AlwI GGATC 2 cut(s) 214, 841
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 1 cut(s) 25
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 3 cut(s) 8, 78, 255
AspS9I GGNCC 2 cut(s) 235, 1000
AsuC2I CCSGG 1 cut(s) 967
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 2 cut(s) 235, 1000
AvrII CCTAGG 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 485
BanI GGYRCC 2 cut(s) 70, 482
BanII GRGCYC 1 cut(s) 420
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 420
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 1 cut(s) 12
BccI CCATC 3 cut(s) 200, 686, 713
BciT130I CCWGG 2 cut(s) 779, 1004
BciVI GTATCC 1 cut(s) 547
BcnI CCSGG 1 cut(s) 967
BcoDI GTCTC 2 cut(s) 746, 764
BfaI CTAG 3 cut(s) 44, 296, 698
BfoI RGCGCY 1 cut(s) 9
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 6 cut(s) 26, 74, 227, 599, 811, 814
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 6 cut(s) 27, 75, 228, 600, 812, 815
Bme1390I CCNGG 3 cut(s) 779, 967, 1004
Bme18I GGWCC 2 cut(s) 235, 1000
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 2 cut(s) 235, 1000
BmiI GGNNCC 4 cut(s) 72, 236, 484, 1008
BmrFI CCNGG 3 cut(s) 779, 967, 1004
BmsI GCATC 3 cut(s) 337, 697, 979
BoxI GACNNNNGTC 2 cut(s) 113, 693
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 2 cut(s) 230, 684
BpuEI CTTGAG 3 cut(s) 155, 224, 877
BpuMI CCSGG 1 cut(s) 967
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 264, 295, 599, 965
BsaWI WCCGGW 2 cut(s) 110, 904
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 1017
Bse3DI GCAATG 1 cut(s) 818
BseBI CCWGG 2 cut(s) 779, 1004
BseDI CCNNGG 4 cut(s) 264, 295, 599, 965
BseGI GGATG 4 cut(s) 352, 475, 712, 724
BseLI CCNNNNNNNGG 1 cut(s) 1017
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 2 cut(s) 244, 675
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 1 cut(s) 485
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 1 cut(s) 12
Bsh1236I CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
Bsh1285I CGRYCG 3 cut(s) 36, 111, 814
BshFI GGCC 1 cut(s) 813
BshNI GGYRCC 2 cut(s) 70, 482
BsiEI CGRYCG 3 cut(s) 36, 111, 814
BsiHKAI GWGCWC 1 cut(s) 420
BsiSI CCGG 5 cut(s) 111, 223, 551, 905, 967
BslFI GGGAC 4 cut(s) 173, 185, 248, 881
BslI CCNNNNNNNGG 1 cut(s) 1017
BsmAI GTCTC 2 cut(s) 746, 764
BsmFI GGGAC 4 cut(s) 173, 185, 248, 881
BsnI GGCC 1 cut(s) 813
Bso31I GGTCTC 1 cut(s) 746
Bsp1286I GDGCHC 2 cut(s) 420, 485
Bsp143I GATC 2 cut(s) 219, 833
Bsp68I TCGCGA 1 cut(s) 291
BspANI GGCC 1 cut(s) 813
BspCNI CTCAG 2 cut(s) 243, 676
BspFNI CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
BspLI GGNNCC 4 cut(s) 72, 236, 484, 1008
BspPI GGATC 2 cut(s) 214, 841
BspT107I GGYRCC 2 cut(s) 70, 482
BspTNI GGTCTC 1 cut(s) 746
BsrBI CCGCTC 1 cut(s) 816
BsrDI GCAATG 1 cut(s) 818
BssECI CCNNGG 4 cut(s) 264, 295, 599, 965
BssMI GATC 2 cut(s) 219, 833
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 779, 1004
Bst4CI ACNGT 4 cut(s) 97, 199, 749, 917
Bst6I CTCTTC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 230, 684
BstDSI CCRYGG 2 cut(s) 264, 599
BstEII GGTNACC 1 cut(s) 631
BstF5I GGATG 4 cut(s) 352, 475, 712, 724
BstFNI CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 3 cut(s) 8, 78, 255
BstKTI GATC 2 cut(s) 222, 836
BstMAI GTCTC 2 cut(s) 746, 764
BstMBI GATC 2 cut(s) 219, 833
BstMCI CGRYCG 3 cut(s) 36, 111, 814
BstMWI GCNNNNNNNGC 3 cut(s) 73, 82, 1004
BstNI CCWGG 2 cut(s) 779, 1004
BstPAI GACNNNNGTC 2 cut(s) 113, 693
BstPI GGTNACC 1 cut(s) 631
BstSCI CCNGG 3 cut(s) 777, 965, 1002
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
BstV1I GCAGC 1 cut(s) 12
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 219, 833
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 2 cut(s) 219, 833
BstZI CGGCCG 1 cut(s) 811
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 1 cut(s) 813
BtgI CCRYGG 2 cut(s) 264, 599
BtgZI GCGATG 1 cut(s) 738
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 4 cut(s) 352, 475, 712, 724
BtsIMutI CAGTG 3 cut(s) 630, 745, 1000
BtuMI TCGCGA 1 cut(s) 291
CciNI GCGGCCGC 1 cut(s) 811
CfoI GCGC 3 cut(s) 8, 78, 255
Cfr13I GGNCC 2 cut(s) 235, 1000
Cfr42I CCGCGG 1 cut(s) 602
CseI GACGC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 388, 439
CviAII CATG 1 cut(s) 864
CviQI GTAC 2 cut(s) 388, 439
DdeI CTNAG 2 cut(s) 230, 684
DpnI GATC 2 cut(s) 221, 835
DpnII GATC 2 cut(s) 219, 833
EaeI YGGCCR 1 cut(s) 811
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 235, 1000
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 418
Eco91I GGTNACC 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 418
EcoO109I RGGNCCY 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 777, 1002
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 867
FaiI YATR 7 cut(s) 639, 644, 775, 789, 808, 855, 865
FaqI GGGAC 4 cut(s) 173, 185, 248, 881
FatI CATG 1 cut(s) 863
FauI CCCGC 2 cut(s) 308, 594
FblI GTMKAC 2 cut(s) 107, 132
Fnu4HI GCNGC 6 cut(s) 26, 74, 227, 599, 811, 814
FokI GGATG 4 cut(s) 359, 482, 719, 731
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 6 cut(s) 26, 74, 227, 599, 811, 814
FspBI CTAG 3 cut(s) 44, 296, 698
GlaI GCGC 3 cut(s) 7, 77, 254
GluI GCNGC 6 cut(s) 26, 74, 227, 599, 811, 814
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 813
HapII CCGG 5 cut(s) 111, 223, 551, 905, 967
HgaI GACGC 1 cut(s) 163
HhaI GCGC 3 cut(s) 8, 78, 255
Hin1II CATG 1 cut(s) 867
Hin6I GCGC 3 cut(s) 6, 76, 253
HinP1I GCGC 3 cut(s) 6, 76, 253
HincII GTYRAC 2 cut(s) 108, 133
HindII GTYRAC 2 cut(s) 108, 133
HinfI GANTC 7 cut(s) 20, 114, 286, 464, 670, 879, 888
HpaII CCGG 5 cut(s) 111, 223, 551, 905, 967
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 3 cut(s) 100, 108, 133
Hpy188I TCNGA 6 cut(s) 33, 306, 469, 518, 887, 951
Hpy188III TCNNGA 9 cut(s) 118, 172, 217, 241, 283, 290, 374, 461, 894
Hpy8I GTNNAC 3 cut(s) 100, 108, 133
Hpy99I CGWCG 5 cut(s) 109, 134, 137, 260, 943
HpyAV CCTTC 7 cut(s) 346, 422, 484, 523, 805, 820, 824
HpyCH4III ACNGT 4 cut(s) 97, 199, 749, 917
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 2 cut(s) 710, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 82, 1004
HpyF3I CTNAG 2 cut(s) 230, 684
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 1 cut(s) 867
HspAI GCGC 3 cut(s) 6, 76, 253
KspI CCGCGG 1 cut(s) 602
Kzo9I GATC 2 cut(s) 219, 833
LmnI GCTCC 3 cut(s) 907, 967, 1012
Lsp1109I GCAGC 1 cut(s) 12
LweI GCATC 3 cut(s) 337, 697, 979
MaeI CTAG 3 cut(s) 44, 296, 698
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 5 cut(s) 118, 326, 579, 631, 859
MalI GATC 2 cut(s) 221, 835
MbiI CCGCTC 1 cut(s) 816
MboI GATC 2 cut(s) 219, 833
MboII GAAGA 8 cut(s) 154, 218, 347, 350, 353, 600, 673, 874
MflI RGATCY 2 cut(s) 219, 833
MhlI GDGCHC 2 cut(s) 420, 485
MluCI AATT 3 cut(s) 732, 839, 989
MluI ACGCGT 1 cut(s) 979
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 2 cut(s) 56, 339
MseI TTAA 4 cut(s) 435, 675, 731, 1023
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 5 cut(s) 111, 223, 551, 905, 967
MspR9I CCNGG 3 cut(s) 779, 967, 1004
MvaI CCWGG 2 cut(s) 779, 1004
MvnI CGCG 6 cut(s) 76, 78, 255, 291, 601, 981
MwoI GCNNNNNNNGC 3 cut(s) 73, 82, 1004
NciI CCSGG 1 cut(s) 967
NdeII GATC 2 cut(s) 219, 833
NlaIII CATG 1 cut(s) 867
NlaIV GGNNCC 4 cut(s) 72, 236, 484, 1008
NmuCI GTSAC 3 cut(s) 118, 326, 579
NotI GCGGCCGC 1 cut(s) 811
NruI TCGCGA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 4 cut(s) 29, 101, 279, 288
PfeI GAWTC 6 cut(s) 20, 286, 464, 670, 879, 888
PkrI GCNGC 6 cut(s) 27, 75, 228, 600, 812, 815
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 2 cut(s) 113, 693
Psp124BI GAGCTC 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 777, 1002
PspEI GGTNACC 1 cut(s) 631
PspGI CCWGG 2 cut(s) 777, 1002
PspN4I GGNNCC 4 cut(s) 72, 236, 484, 1008
PspPI GGNCC 2 cut(s) 235, 1000
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 2 cut(s) 219, 833
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 389, 440
RsaNI GTAC 2 cut(s) 388, 439
SacI GAGCTC 1 cut(s) 420
SacII CCGCGG 1 cut(s) 602
SalI GTCGAC 2 cut(s) 106, 131
SaqAI TTAA 4 cut(s) 435, 675, 731, 1023
SatI GCNGC 6 cut(s) 26, 74, 227, 599, 811, 814
Sau3AI GATC 2 cut(s) 219, 833
Sau96I GGNCC 2 cut(s) 235, 1000
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 3 cut(s) 779, 967, 1004
SduI GDGCHC 2 cut(s) 420, 485
SfaNI GCATC 3 cut(s) 337, 697, 979
Sfr303I CCGCGG 1 cut(s) 602
SgrBI CCGCGG 1 cut(s) 602
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 2 cut(s) 235, 1000
SmlI CTYRAG 3 cut(s) 170, 239, 892
SmoI CTYRAG 3 cut(s) 170, 239, 892
Sse9I AATT 3 cut(s) 732, 839, 989
SspMI CTAG 3 cut(s) 44, 296, 698
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 777, 965, 1002
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 97, 199, 749, 917
TaiI ACGT 1 cut(s) 333
TaqI TCGA 6 cut(s) 107, 132, 162, 426, 477, 533
TaqII GACCGA 1 cut(s) 50
TasI AATT 3 cut(s) 732, 839, 989
TatI WGTACW 2 cut(s) 387, 438
TauI GCSGC 5 cut(s) 76, 229, 601, 813, 816
TfiI GAWTC 6 cut(s) 20, 286, 464, 670, 879, 888
Tru1I TTAA 4 cut(s) 435, 675, 731, 1023
Tru9I TTAA 4 cut(s) 435, 675, 731, 1023
TscAI CASTG 3 cut(s) 630, 752, 1000
TseFI GTSAC 3 cut(s) 118, 326, 579
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 3 cut(s) 118, 326, 579
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 3 cut(s) 175, 567, 891
TspRI CASTG 3 cut(s) 630, 752, 1000
VpaK11BI GGWCC 2 cut(s) 235, 1000
XmaJI CCTAGG 1 cut(s) 295
XmiI GTMKAC 2 cut(s) 107, 132
XspI CTAG 3 cut(s) 44, 296, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.