Rh6DG487500

A-kinase anchor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010497
Physical Location & Seq
Forward (+)
64881298 .. 64885527
4230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG487500.1

Sequence Viewer

Length: 723 bp
ATGAGCACGCTGAAGTTGGATTCGATTTCTCCTACTGAAACCGTGGAGATAGAGAATGGTCTGTCACTGGTGCCGCGGCTCCGGCTGAATATTACGGTGTATCCGTCGAGCCAAGTTGTGAGCAAACCGATTGACGAGTGGAAGATGAAGCAAACCCTAATTGACTTCCTCAAGAACTCGCTTTCCGATCCGGTCACAGTTCCGGAGGAGGATCTCAAGATCAGACGGGTGAGGGACCTGAAGAAGCGGAAGCGGGACGACCCTGTAGCTTCCGGTACCATCTTCATTCGCGACCTAGGGTTTCTGAGGAAGACGAGTAGGAAGGGTGAGGAGGTGGAGAAGAAGTTCAGTGATTGGAGGAGGTACATTGTGGAGAAGATGGATGGGATTGAGCTCAATCTCGAAGGGGTTAAGTTCAAGCTAAACGTCGCCGTTCCGGAGTCGGATGATTTCCAGGGGATGAAGAAAGGTTGGGAGGAGTTTTACGCTTTCGGAAATCGAGGGTTTTCGAGGGGTGGGAAGCAAGAGACTGATACTATAATACTGAGGGGTGTTCCTTCACGCTGGTTCGCAGAGCCACGGGTTTCGTCTAAGCCTTCAATGCTGGTTACACATACCATCTTTTCGACATTTGGGAAGATAAGGCATGTCAATGTGTTTGTTTTCTTGCTCTATCTGTTATCATATTGTAGAAAGCTACAGAGAAAATATTATGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.94

Weight (kDa)

9.7

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_AKAP-17A PF25015 113 - 219 3.2e-21 AKAP-17A-like, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 70, 275
AccII CGCG 2 cut(s) 76, 291
AccIII TCCGGA 2 cut(s) 202, 436
AciI CCGC 4 cut(s) 74, 76, 247, 253
AclWI GGATC 2 cut(s) 182, 219
AcuI CTGAAG 2 cut(s) 32, 260
AfaI GTAC 2 cut(s) 277, 365
AgsI TTSAA 2 cut(s) 418, 600
AjnI CCWGG 1 cut(s) 453
AloI GAACNNNNNNTCC 4 cut(s) 167, 199, 329, 361
AluBI AGCT 4 cut(s) 269, 394, 421, 697
AluI AGCT 4 cut(s) 269, 394, 421, 697
Alw21I GWGCWC 2 cut(s) 8, 396
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 2 cut(s) 182, 219
Aor13HI TCCGGA 2 cut(s) 202, 436
Asp700I GAANNNNTTC 1 cut(s) 344
Asp718I GGTACC 1 cut(s) 275
AspA2I CCTAGG 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 241, 338
AvaII GGWCC 1 cut(s) 235
AvrII CCTAGG 1 cut(s) 295
BanI GGYRCC 2 cut(s) 70, 275
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 8, 396
BccI CCATC 4 cut(s) 287, 373, 377, 626
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 455
BciVI GTATCC 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 264, 698
BfuI GTATCC 1 cut(s) 111
BisI GCNGC 2 cut(s) 74, 77
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 2 cut(s) 75, 78
Bme1390I CCNGG 1 cut(s) 455
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 4 cut(s) 72, 80, 236, 277
BmrFI CCNGG 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 317
BpuEI CTTGAG 2 cut(s) 155, 200
BsaJI CCNNGG 5 cut(s) 42, 74, 295, 454, 578
BsaWI WCCGGW 4 cut(s) 190, 202, 272, 436
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse1I ACTGG 1 cut(s) 72
BseAI TCCGGA 2 cut(s) 202, 436
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 5 cut(s) 42, 74, 295, 454, 578
BseGI GGATG 3 cut(s) 388, 451, 465
BseMII CTCAG 2 cut(s) 296, 536
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 4 cut(s) 221, 344, 373, 491
Bsh1236I CGCG 2 cut(s) 76, 291
BshNI GGYRCC 2 cut(s) 70, 275
BsiHKAI GWGCWC 2 cut(s) 8, 396
BsiSI CCGG 5 cut(s) 82, 191, 203, 273, 437
BslFI GGGAC 2 cut(s) 248, 269
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 2 cut(s) 248, 269
Bsp1286I GDGCHC 2 cut(s) 8, 396
Bsp13I TCCGGA 2 cut(s) 202, 436
Bsp143I GATC 3 cut(s) 187, 211, 219
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 4 cut(s) 74, 76, 247, 253
BspCNI CTCAG 2 cut(s) 297, 537
BspEI TCCGGA 2 cut(s) 202, 436
BspFNI CGCG 2 cut(s) 76, 291
BspLI GGNNCC 4 cut(s) 72, 80, 236, 277
BspPI GGATC 2 cut(s) 182, 219
BspT107I GGYRCC 2 cut(s) 70, 275
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 5 cut(s) 42, 74, 295, 454, 578
BssMI GATC 3 cut(s) 187, 211, 219
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 3 cut(s) 43, 97, 199
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 3 cut(s) 305, 545, 591
BstDSI CCRYGG 3 cut(s) 42, 74, 578
BstF5I GGATG 3 cut(s) 388, 451, 465
BstFNI CGCG 2 cut(s) 76, 291
BstKTI GATC 3 cut(s) 190, 214, 222
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 3 cut(s) 187, 211, 219
BstMWI GCNNNNNNNGC 2 cut(s) 82, 601
BstNI CCWGG 1 cut(s) 455
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 1 cut(s) 453
BstSFI CTRYAG 2 cut(s) 264, 698
BstUI CGCG 2 cut(s) 76, 291
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuI GTATCC 1 cut(s) 111
BtgI CCRYGG 3 cut(s) 42, 74, 578
BtsCI GGATG 3 cut(s) 388, 451, 465
BtsIMutI CAGTG 2 cut(s) 65, 355
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 77
Csp6I GTAC 2 cut(s) 276, 364
CviAII CATG 1 cut(s) 647
CviJI RGCY 9 cut(s) 79, 85, 111, 269, 394, 421, 577, 595, 697
CviKI_1 RGCY 9 cut(s) 79, 85, 111, 269, 394, 421, 577, 595, 697
CviQI GTAC 2 cut(s) 276, 364
DdeI CTNAG 3 cut(s) 305, 545, 591
DpnI GATC 3 cut(s) 189, 213, 221
DpnII GATC 3 cut(s) 187, 211, 219
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 32, 260
EcoICRI GAGCTC 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 650
FaiI YATR 5 cut(s) 539, 615, 648, 685, 714
FaqI GGGAC 2 cut(s) 248, 269
FatI CATG 1 cut(s) 646
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 74, 77
FokI GGATG 3 cut(s) 395, 458, 472
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 2 cut(s) 74, 77
FspBI CTAG 1 cut(s) 296
GluI GCNGC 2 cut(s) 74, 77
HapII CCGG 5 cut(s) 82, 191, 203, 273, 437
Hin1II CATG 1 cut(s) 650
HinfI GANTC 2 cut(s) 20, 440
HpaII CCGG 5 cut(s) 82, 191, 203, 273, 437
HphI GGTGA 2 cut(s) 241, 338
Hpy188I TCNGA 5 cut(s) 187, 224, 306, 445, 494
Hpy188III TCNNGA 6 cut(s) 172, 203, 217, 290, 401, 437
Hpy99I CGWCG 2 cut(s) 109, 431
HpyAV CCTTC 4 cut(s) 316, 398, 567, 606
HpyCH4III ACNGT 3 cut(s) 43, 97, 199
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 601
HpyF3I CTNAG 3 cut(s) 305, 545, 591
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 1 cut(s) 650
Kpn2I TCCGGA 2 cut(s) 202, 436
KpnI GGTACC 1 cut(s) 279
KspI CCGCGG 1 cut(s) 77
Kzo9I GATC 3 cut(s) 187, 211, 219
LmnI GCTCC 1 cut(s) 84
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 3 cut(s) 63, 193, 607
MalI GATC 3 cut(s) 189, 213, 221
MboI GATC 3 cut(s) 187, 211, 219
MboII GAAGA 8 cut(s) 154, 253, 274, 322, 352, 388, 475, 649
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 8, 396
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 423
MroI TCCGGA 2 cut(s) 202, 436
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 5 cut(s) 82, 191, 203, 273, 437
MspR9I CCNGG 1 cut(s) 455
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 2 cut(s) 76, 291
MwoI GCNNNNNNNGC 2 cut(s) 82, 601
NdeII GATC 3 cut(s) 187, 211, 219
NlaIII CATG 1 cut(s) 650
NlaIV GGNNCC 4 cut(s) 72, 80, 236, 277
NmuCI GTSAC 2 cut(s) 63, 193
NruI TCGCGA 1 cut(s) 291
NspI RCATGY 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 2 cut(s) 75, 78
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 453
PspGI CCWGG 1 cut(s) 453
PspN4I GGNNCC 4 cut(s) 72, 80, 236, 277
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 1 cut(s) 211
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 277, 365
RsaNI GTAC 2 cut(s) 276, 364
RseI CAYNNNNRTG 1 cut(s) 651
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 77
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 74, 77
Sau3AI GATC 3 cut(s) 187, 211, 219
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 455
SduI GDGCHC 2 cut(s) 8, 396
SfcI CTRYAG 2 cut(s) 264, 698
Sfr303I CCGCGG 1 cut(s) 77
SgrBI CCGCGG 1 cut(s) 77
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 2 cut(s) 170, 215
SmoI CTYRAG 2 cut(s) 170, 215
Sse9I AATT 1 cut(s) 159
SsiI CCGC 4 cut(s) 74, 76, 247, 253
SspI AATATT 2 cut(s) 91, 710
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 453
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 3 cut(s) 43, 97, 199
TaiI ACGT 1 cut(s) 429
TaqI TCGA 6 cut(s) 23, 107, 402, 499, 509, 626
TasI AATT 1 cut(s) 159
TauI GCSGC 2 cut(s) 76, 79
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 72, 355
TseFI GTSAC 2 cut(s) 63, 193
Tsp45I GTSAC 2 cut(s) 63, 193
TspDTI ATGAA 3 cut(s) 161, 274, 476
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 72, 355
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 1 cut(s) 650
XmaJI CCTAGG 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.