FvH4_2g34740

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008219
Physical Location & Seq
Reverse (-)
25726760 .. 25728215
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34740.t1

Sequence Viewer

Length: 378 bp
ATGTTGCTTAACCTTCACAAGAAGAAATGGACTGATGGGCTGACCCTAAAACGTTTTGATACTCATTCAAAAACAAATGAGCGAACTGTTCAGCAAGTATGGAAGTTGCTGATTTTGATTGATGTATGGGGTACATACTATTTAGTGCACAGAGTCATTGTTCTTTCTTTCATTTGGTCTCTGCTGGAGATGTTGAGCCTAGCTGTCAAGTACAACAAGGCCGTGCAGGAGGAGGATGAGTTGTCTCCAGAGAAGCTTGCTATTGCAAATGTGGGAAGACAAGATGCCAAGAAGCACCTAGAAGAACATGTCTCCAACTTAATGTCCTCAAACATAGTCCAGACCTTGGGAACCATGCTCGATACTGTTGTGTTCTAG

Protein Analysis

126

Amino Acids

14.51

Weight (kDa)

8.0

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPN11_C PF23594 62 - 123 1.6e-28 26S proteasome regulatory subunit RPN11 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 346
AclI AACGTT 1 cut(s) 52
AfaI GTAC 2 cut(s) 133, 212
AfiI CCNNNNNNNGG 1 cut(s) 346
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 1 cut(s) 69
AluBI AGCT 2 cut(s) 203, 256
AluI AGCT 2 cut(s) 203, 256
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 183, 249, 316
Alw44I GTGCAC 1 cut(s) 146
AoxI GGCC 1 cut(s) 219
ApaLI GTGCAC 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 150
BaeI ACNNNNGTAYC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 206
BcoDI GTCTC 3 cut(s) 183, 249, 316
BfaI CTAG 3 cut(s) 200, 299, 376
BmiI GGNNCC 1 cut(s) 352
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 283
BpmI CTGGAG 2 cut(s) 206, 231
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 345
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 346
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 3 cut(s) 183, 249, 316
BsnI GGCC 1 cut(s) 221
Bso31I GGTCTC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 150
BspANI GGCC 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 88, 367
BstC8I GCNNGC 1 cut(s) 258
BstF5I GGATG 1 cut(s) 241
BstMAI GTCTC 3 cut(s) 183, 249, 316
BstNSI RCATGY 1 cut(s) 311
BstSLI GKGCMC 1 cut(s) 150
BstV2I GAAGAC 1 cut(s) 283
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 258
Csp6I GTAC 2 cut(s) 132, 211
CviAII CATG 2 cut(s) 308, 355
CviJI RGCY 5 cut(s) 40, 198, 203, 221, 256
CviKI_1 RGCY 5 cut(s) 40, 198, 203, 221, 256
CviQI GTAC 2 cut(s) 132, 211
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 2 cut(s) 311, 358
FaiI YATR 6 cut(s) 100, 127, 136, 309, 335, 356
FatI CATG 2 cut(s) 307, 354
FokI GGATG 1 cut(s) 248
FspBI CTAG 3 cut(s) 200, 299, 376
GsuI CTGGAG 2 cut(s) 206, 231
HaeIII GGCC 1 cut(s) 221
Hin1II CATG 2 cut(s) 311, 358
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 248, 340
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 2 cut(s) 88, 367
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 148, 226, 266
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 2 cut(s) 311, 358
LpnPI CCDG 4 cut(s) 170, 212, 261, 353
LweI GCATC 1 cut(s) 274
MaeI CTAG 3 cut(s) 200, 299, 376
MaeII ACGT 1 cut(s) 52
MboII GAAGA 3 cut(s) 34, 288, 314
MhlI GDGCHC 1 cut(s) 150
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 3 cut(s) 223, 226, 337
MseI TTAA 2 cut(s) 9, 320
NlaIII CATG 2 cut(s) 311, 358
NlaIV GGNNCC 1 cut(s) 352
NspI RCATGY 1 cut(s) 311
PciI ACATGT 1 cut(s) 307
PflMI CCANNNNNTGG 1 cut(s) 346
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PscI ACATGT 1 cut(s) 307
Psp1406I AACGTT 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 352
RsaI GTAC 2 cut(s) 133, 212
RsaNI GTAC 2 cut(s) 132, 211
SaqAI TTAA 2 cut(s) 9, 320
SchI GAGTC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 150
SetI ASST 6 cut(s) 15, 55, 205, 258, 300, 347
SfaNI GCATC 1 cut(s) 274
SspMI CTAG 3 cut(s) 200, 299, 376
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 88, 367
TaiI ACGT 1 cut(s) 55
TaqI TCGA 1 cut(s) 360
TatI WGTACW 1 cut(s) 210
Tru1I TTAA 2 cut(s) 9, 320
Tru9I TTAA 2 cut(s) 9, 320
TspDTI ATGAA 1 cut(s) 160
Van91I CCANNNNNTGG 1 cut(s) 346
VneI GTGCAC 1 cut(s) 146
XceI RCATGY 1 cut(s) 311
XspI CTAG 3 cut(s) 200, 299, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.