FvH4_2g34940

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015304
Physical Location & Seq
Forward (+)
25860295 .. 25862112
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34940.t1

Sequence Viewer

Length: 1047 bp
ATGGAGGTAGCTATACCGAGCAGACCCGCTTTCGATTTCGACTTCAACAGCGCTCGATCTTCCCCTGCCATCACTGCTCCTTCTACTCCGAGACGCTTTGGCGACTACAGCTTCTTGAGTGCACCAACCAGTCCATCTAGAATATCTGAGTTTTACAGAGAATTTGATGAGTTCTCCGCAGTCAGTAGTAACTGGGAAGAAGAGGATGAGTTTGAACGTGCAGCACCATCCAAATCATCAAAACCAGACGACTTTGCCTTTGATATTGGTGAGGAGGTAGACAGAGTTTCTCTCTCAGCTGAAGAGCTCTTTGATGGCGGTGTTATAAGACCTTTAAAGCTTCCTTCACCTCCTCAATCCTCAACAAAGAAGATGAATGTTCGGCAAGCTCTTTCGCCCAGGAGGAGGAAAGAAAGAGATTCATATCCATCCGGGATGAACAATCCGACCAGAATGGAAACTCCCCAGAAGAAATCAACTGTTTCTTCACAGCAACAGAGAGGAAGAGAAAGAGCTTCCTCAACCTTGCCTTCTTCGAGCCGTAGAGCTTCAAGATCACTATCTCCTTTGAGGGTTTCAGAGTTTAACTGGGCACAAGAAGAAGAAGAGCTGAAACGACAGCAAGTAGCAGCACAGAACGAGAACAAGCAACTAGCTACAAAGGGTTCACTAATTTCATCTGCTACTGCTGCTCTAAATAGTTGCAAGGCTTCAAGAAAATGGAGGCTGAAAGATTTCCTGTTGTTTCGGAGCGCATCAGAGGGCAGAGCAACAGATAAAGATCCATTCAGCAAGTTCTCGAATCATAAAAATGAGCATGTGAAGACTGCTAGCTCCAGCTTCAGATCCATAGACAGCCCGGCCTCTGTTTCAAGAAGAAAAGGACCTTCAGCACATGAGTTGCATTACACCGTGAACAAGGCAGTGTCCAATGACATGAAGAAGAAGACCTTCCTGCCTTACAATCAGGGCATTCTAGGAAGACTAGCTTTCAATCCTGCTGTTCATTCTCTGTCCACTGGATTTGGGTCTCTTTCAAGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

349

Amino Acids

38.83

Weight (kDa)

9.87

Isoelectric Point (pI)

74.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 100 - 320 2e-39 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26530 AT2G26530
fragaria_vesca FvH4_2g34940
malus_domestica MD08G1106900.v1.1 MD15G1087600.v1.1
prunus_persica Prupe.1G441300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0307061
rosa_laevigata RLG00000010737
rosa_multiflora Rmu_sc0005634.1_g000005
rosa_roxburghii Rroxscaffold_7G00161190
rosa_rugosa Rorug06G0355600
rosa_samantha Rh6AG467100 Rh6BG433400 Rh6CG482600 Rh6DG468900
rosa_wichuraiana Rw6G040720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccI GTMKAC 1 cut(s) 279
AciI CCGC 3 cut(s) 27, 177, 318
AclWI GGATC 2 cut(s) 776, 840
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 3 cut(s) 321, 826, 873
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 1 cut(s) 405
AgsI TTSAA 7 cut(s) 46, 215, 552, 714, 873, 994, 1038
AjnI CCWGG 1 cut(s) 398
Alw21I GWGCWC 2 cut(s) 124, 309
Alw26I GTCTC 2 cut(s) 85, 1035
Alw44I GTGCAC 1 cut(s) 120
AlwI GGATC 2 cut(s) 776, 840
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 1 cut(s) 861
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 3 cut(s) 221, 629, 689
ApoI RAATTY 1 cut(s) 161
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 2 cut(s) 734, 950
AspLEI GCGC 2 cut(s) 53, 755
AspS9I GGNCC 2 cut(s) 884, 1041
AsuC2I CCSGG 2 cut(s) 433, 860
AsuHPI GGTGA 2 cut(s) 281, 339
AsuNHI GCTAGC 1 cut(s) 830
AvaII GGWCC 2 cut(s) 884, 1041
BaeGI GKGCMC 2 cut(s) 124, 595
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 3 cut(s) 830, 953, 988
Bbv12I GWGCWC 2 cut(s) 124, 309
BbvI GCAGC 3 cut(s) 233, 641, 676
BccI CCATC 5 cut(s) 77, 142, 235, 308, 436
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 400
BcnI CCSGG 2 cut(s) 433, 860
BcoDI GTCTC 2 cut(s) 85, 1035
BfaI CTAG 5 cut(s) 138, 653, 831, 977, 986
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 3 cut(s) 222, 630, 690
BlsI GCNGC 3 cut(s) 223, 631, 691
Bme1390I CCNGG 3 cut(s) 400, 433, 860
Bme18I GGWCC 2 cut(s) 884, 1041
BmgT120I GGNCC 2 cut(s) 884, 1041
BmrFI CCNGG 3 cut(s) 400, 433, 860
BmrI ACTGGG 2 cut(s) 202, 598
BmsI GCATC 1 cut(s) 764
BmtI GCTAGC 1 cut(s) 834
BmuI ACTGGG 2 cut(s) 202, 598
BpiI GAAGAC 3 cut(s) 830, 953, 988
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 1 cut(s) 820
BpuEI CTTGAG 1 cut(s) 136
BpuMI CCSGG 2 cut(s) 433, 860
BsaBI GATNNNNATC 3 cut(s) 423, 559, 780
BsaI GGTCTC 1 cut(s) 1035
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 405
Bse1I ACTGG 4 cut(s) 129, 197, 593, 1024
Bse8I GATNNNNATC 3 cut(s) 423, 559, 780
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 4 cut(s) 211, 227, 428, 441
BseJI GATNNNNATC 3 cut(s) 423, 559, 780
BseLI CCNNNNNNNGG 1 cut(s) 405
BseMII CTCAG 2 cut(s) 138, 309
BseNI ACTGG 4 cut(s) 129, 197, 593, 1024
BseRI GAGGAG 3 cut(s) 287, 342, 418
BseSI GKGCMC 2 cut(s) 124, 595
BseXI GCAGC 3 cut(s) 233, 641, 676
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 1 cut(s) 863
BsiHKAI GWGCWC 2 cut(s) 124, 309
BsiSI CCGG 2 cut(s) 432, 860
BslI CCNNNNNNNGG 1 cut(s) 405
BsmAI GTCTC 2 cut(s) 85, 1035
BsmBI CGTCTC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 972
BsnI GGCC 1 cut(s) 863
Bso31I GGTCTC 1 cut(s) 1035
Bsp1286I GDGCHC 3 cut(s) 124, 309, 595
Bsp143I GATC 4 cut(s) 56, 554, 781, 845
BspACI CCGC 3 cut(s) 27, 177, 318
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 2 cut(s) 139, 308
BspOI GCTAGC 1 cut(s) 834
BspPI GGATC 2 cut(s) 776, 840
BspQI GCTCTTC 2 cut(s) 297, 600
BspTNI GGTCTC 1 cut(s) 1035
BsrI ACTGG 4 cut(s) 129, 197, 593, 1024
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 4 cut(s) 56, 554, 781, 845
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 2 cut(s) 481, 913
Bst6I CTCTTC 4 cut(s) 195, 297, 499, 600
BstC8I GCNNGC 2 cut(s) 387, 832
BstDEI CTNAG 2 cut(s) 147, 295
BstF5I GGATG 4 cut(s) 211, 227, 428, 441
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 755
BstKTI GATC 4 cut(s) 59, 557, 784, 848
BstMAI GTCTC 2 cut(s) 85, 1035
BstMBI GATC 4 cut(s) 56, 554, 781, 845
BstMWI GCNNNNNNNGC 3 cut(s) 74, 108, 689
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 821
BstSCI CCNGG 3 cut(s) 398, 431, 858
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 2 cut(s) 124, 595
BstV1I GCAGC 3 cut(s) 233, 641, 676
BstV2I GAAGAC 3 cut(s) 830, 953, 988
BstX2I RGATCY 2 cut(s) 781, 845
BstYI RGATCY 2 cut(s) 781, 845
BsuRI GGCC 1 cut(s) 863
BtsCI GGATG 4 cut(s) 211, 227, 428, 441
BtsI GCAGTG 2 cut(s) 72, 930
BtsIMutI CAGTG 3 cut(s) 72, 930, 1017
Cac8I GCNNGC 2 cut(s) 387, 832
CfoI GCGC 2 cut(s) 53, 755
Cfr13I GGNCC 2 cut(s) 884, 1041
CseI GACGC 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 1006, 1041
CviAII CATG 3 cut(s) 818, 896, 937
DdeI CTNAG 2 cut(s) 147, 295
DpnI GATC 4 cut(s) 58, 556, 783, 847
DpnII GATC 4 cut(s) 56, 554, 781, 845
DraI TTTAAA 1 cut(s) 336
Eam1104I CTCTTC 4 cut(s) 195, 297, 499, 600
EarI CTCTTC 4 cut(s) 195, 297, 499, 600
Ecl136II GAGCTC 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 1035
Eco47I GGWCC 2 cut(s) 884, 1041
Eco47III AGCGCT 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 3 cut(s) 321, 826, 873
EcoICRI GAGCTC 1 cut(s) 307
EcoO109I RGGNCCY 2 cut(s) 884, 1041
EcoRII CCWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 3 cut(s) 821, 899, 940
FaiI YATR 8 cut(s) 14, 326, 424, 807, 819, 851, 897, 938
FalI AAGNNNNNCTT 4 cut(s) 935, 967, 973, 1005
FatI CATG 3 cut(s) 817, 895, 936
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 222, 630, 690
FokI GGATG 4 cut(s) 214, 218, 415, 448
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 3 cut(s) 222, 630, 690
FspBI CTAG 5 cut(s) 138, 653, 831, 977, 986
GlaI GCGC 2 cut(s) 52, 754
GluI GCNGC 3 cut(s) 222, 630, 690
GsuI CTGGAG 1 cut(s) 820
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 863
HapII CCGG 2 cut(s) 432, 860
HgaI GACGC 1 cut(s) 102
HhaI GCGC 2 cut(s) 53, 755
Hin1II CATG 3 cut(s) 821, 899, 940
Hin6I GCGC 2 cut(s) 51, 753
HinP1I GCGC 2 cut(s) 51, 753
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 419, 802
HpaII CCGG 2 cut(s) 432, 860
HphI GGTGA 2 cut(s) 281, 339
Hpy166II GTNNAC 5 cut(s) 122, 280, 668, 916, 1017
Hpy188I TCNGA 7 cut(s) 90, 148, 447, 580, 750, 760, 845
Hpy188III TCNNGA 7 cut(s) 115, 138, 552, 714, 799, 873, 1044
Hpy8I GTNNAC 5 cut(s) 122, 280, 668, 916, 1017
HpyAV CCTTC 5 cut(s) 90, 354, 540, 897, 961
HpyCH4III ACNGT 2 cut(s) 481, 913
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 4 cut(s) 122, 221, 705, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 108, 689
HpyF3I CTNAG 2 cut(s) 147, 295
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 3 cut(s) 821, 899, 940
HspAI GCGC 2 cut(s) 51, 753
Kzo9I GATC 4 cut(s) 56, 554, 781, 845
LguI GCTCTTC 2 cut(s) 297, 600
LmnI GCTCC 3 cut(s) 82, 750, 839
Lsp1109I GCAGC 3 cut(s) 233, 641, 676
LweI GCATC 1 cut(s) 764
MaeI CTAG 5 cut(s) 138, 653, 831, 977, 986
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 1 cut(s) 188
MalI GATC 4 cut(s) 58, 556, 783, 847
MboI GATC 4 cut(s) 56, 554, 781, 845
MflI RGATCY 2 cut(s) 781, 845
MhlI GDGCHC 3 cut(s) 124, 309, 595
MluCI AATT 2 cut(s) 161, 672
MmeI TCCRAC 1 cut(s) 470
MroXI GAANNNNTTC 2 cut(s) 734, 950
MseI TTAA 2 cut(s) 335, 585
MslI CAYNNNNRTG 1 cut(s) 810
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 432, 860
MspR9I CCNGG 3 cut(s) 400, 433, 860
Mva1269I GAATGC 1 cut(s) 972
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 3 cut(s) 74, 108, 689
NciI CCSGG 2 cut(s) 433, 860
NdeII GATC 4 cut(s) 56, 554, 781, 845
NheI GCTAGC 1 cut(s) 830
NlaIII CATG 3 cut(s) 821, 899, 940
NspI RCATGY 1 cut(s) 821
PciSI GCTCTTC 2 cut(s) 297, 600
PctI GAATGC 1 cut(s) 972
PdmI GAANNNNTTC 2 cut(s) 734, 950
PfeI GAWTC 2 cut(s) 419, 802
PfoI TCCNGGA 1 cut(s) 431
PkrI GCNGC 3 cut(s) 223, 631, 691
PpuMI RGGWCCY 2 cut(s) 884, 1041
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 309
Psp5II RGGWCCY 2 cut(s) 884, 1041
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspPI GGNCC 2 cut(s) 884, 1041
PspPPI RGGWCCY 2 cut(s) 884, 1041
PsuI RGATCY 2 cut(s) 781, 845
PvuII CAGCTG 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 810
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 2 cut(s) 297, 600
SaqAI TTAA 2 cut(s) 335, 585
SatI GCNGC 3 cut(s) 222, 630, 690
Sau3AI GATC 4 cut(s) 56, 554, 781, 845
Sau96I GGNCC 2 cut(s) 884, 1041
ScrFI CCNGG 3 cut(s) 400, 433, 860
SduI GDGCHC 3 cut(s) 124, 309, 595
SfaNI GCATC 1 cut(s) 764
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 2 cut(s) 884, 1041
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
Sse9I AATT 2 cut(s) 161, 672
SsiI CCGC 3 cut(s) 27, 177, 318
SspMI CTAG 5 cut(s) 138, 653, 831, 977, 986
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 3 cut(s) 398, 431, 858
TaaI ACNGT 2 cut(s) 481, 913
TaiI ACGT 1 cut(s) 220
TaqI TCGA 5 cut(s) 33, 39, 55, 536, 800
TasI AATT 2 cut(s) 161, 672
TfiI GAWTC 2 cut(s) 419, 802
Tru1I TTAA 2 cut(s) 335, 585
Tru9I TTAA 2 cut(s) 335, 585
TscAI CASTG 3 cut(s) 79, 930, 1024
TseI GCWGC 3 cut(s) 221, 629, 689
TspDTI ATGAA 6 cut(s) 389, 411, 452, 666, 953, 995
TspRI CASTG 3 cut(s) 79, 930, 1024
VneI GTGCAC 1 cut(s) 120
VpaK11BI GGWCC 2 cut(s) 884, 1041
XapI RAATTY 1 cut(s) 161
XbaI TCTAGA 1 cut(s) 137
XceI RCATGY 1 cut(s) 821
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 2 cut(s) 734, 950
XspI CTAG 5 cut(s) 138, 653, 831, 977, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.