Rw6G040720

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015304
Physical Location & Seq
Reverse (-)
64489162 .. 64490209
1048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040720.1

Sequence Viewer

Length: 1017 bp
ATGGAGGTAGCTATACCAAGCAGACCCACCTTGGATTTCGACTTCAACAGCGCTCGATCTTCGCCTTGCATTACTACTCCTTCAACTCCGAAACGCTTCGGCGACTACAGCTTCTTGAGTGCACCAACCAGTCCATCTCGAATGTCCGAGTTTTACAGAGAATTTGATGAATTCTCCACAGTCAGTAGTAACTGGGAAGAAGAAGATGAGCCTGAACTTGCAGCACCACCCAAATCACCAAAACCAGACGACTTTGCGTTTGATGTTAGCGAGGAGGTAGACAGAGTTTCTCTCTCAGCTGAAGAGCTTTTCGATGGCGGCGTTATAAGACCTTTAAAGGCTCCTCCACCTCCTCAATTCTCAACAAAGAAGATCAATATTCGTCAAGCTCTTTCGCCCAGAAGGAGAAAAGATAGAGATTCATATCCATTCGCGGCGAACAATCCGACCGGAATGGAAACTCCCCAGAAGAGATCAGCTGTTTCTTCACAGCAACAAAGAGGAAGAGAAAGAGCTCCTCCAACCGTGTCTTCTTCAAGCCGCAGATCTTCAAGATCACTATCTCCTTTGAGGGTTTCAGAGTTTAACTGGGCACAAGAAGAAGAAGAGCTAAAACGACAAGCAGCAGCAGCACAGAACAAGCAATTAGCTACAAAGGGTTCATTAATTTCATCTGCTACTGCTACTTTGAATAGTTGCAAGGCTTCAAGAAAATGGAGGCTGAAAGATTTTCTGTTGTTTCGGAGCGCATCAGAGGGCAGAGCAACAGATAAAGATCCATTCAGAAAGTTCTGGAATCAGAAAAACGAGCATGCGAAGACCGCTAGCTCCAGCTTCAGATCCATAGACAGCCCGGCCTCTGTTTCTAGTTCAAGAAGAAGAGGACCTTCAGCACATGAGTTGCATTACACTAAGAAGACCTTCTTGCCTTACAAACAGGGCATTCTAGGAAGATTGGCTTTCAATCCTGCTGTTCATTCTCTTGCCAATGGATTTGGGTCTCTTTCAAGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

338

Amino Acids

37.75

Weight (kDa)

9.87

Isoelectric Point (pI)

80.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 100 - 306 2.5e-32 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26530 AT2G26530
fragaria_vesca FvH4_2g34940
malus_domestica MD08G1106900.v1.1 MD15G1087600.v1.1
prunus_persica Prupe.1G441300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0307061
rosa_laevigata RLG00000010737
rosa_multiflora Rmu_sc0005634.1_g000005
rosa_roxburghii Rroxscaffold_7G00161190
rosa_rugosa Rorug06G0355600
rosa_samantha Rh6AG467100 Rh6BG433400 Rh6CG482600 Rh6DG468900
rosa_wichuraiana Rw6G040720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 434
AciI CCGC 4 cut(s) 318, 434, 541, 822
AclWI GGATC 2 cut(s) 770, 834
AcsI RAATTY 2 cut(s) 161, 170
AcuI CTGAAG 3 cut(s) 321, 820, 873
AfeI AGCGCT 1 cut(s) 52
AgsI TTSAA 9 cut(s) 46, 84, 537, 552, 691, 708, 873, 964, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
Alw21I GWGCWC 2 cut(s) 124, 517
Alw26I GTCTC 1 cut(s) 1005
Alw44I GTGCAC 1 cut(s) 120
AlwI GGATC 2 cut(s) 770, 834
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 1 cut(s) 855
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 4 cut(s) 221, 623, 626, 629
ApoI RAATTY 2 cut(s) 161, 170
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 1 cut(s) 665
Asp700I GAANNNNTTC 2 cut(s) 95, 920
AspLEI GCGC 2 cut(s) 53, 749
AspS9I GGNCC 2 cut(s) 884, 1011
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 1 cut(s) 228
AsuNHI GCTAGC 1 cut(s) 824
AvaII GGWCC 2 cut(s) 884, 1011
BaeGI GKGCMC 2 cut(s) 124, 595
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 3 cut(s) 522, 824, 923
Bbv12I GWGCWC 2 cut(s) 124, 517
BbvI GCAGC 4 cut(s) 233, 635, 638, 641
BccI CCATC 2 cut(s) 142, 308
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 1 cut(s) 1005
BfaI CTAG 3 cut(s) 825, 867, 947
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 54
BglII AGATCT 1 cut(s) 545
BisI GCNGC 7 cut(s) 222, 319, 435, 541, 624, 627, 630
BlsI GCNGC 7 cut(s) 223, 320, 436, 542, 625, 628, 631
Bme1390I CCNGG 1 cut(s) 854
Bme18I GGWCC 2 cut(s) 884, 1011
BmgT120I GGNCC 2 cut(s) 884, 1011
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 854
BmrI ACTGGG 2 cut(s) 202, 598
BmsI GCATC 1 cut(s) 758
BmtI GCTAGC 1 cut(s) 828
BmuI ACTGGG 2 cut(s) 202, 598
BpiI GAAGAC 3 cut(s) 522, 824, 923
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 1 cut(s) 814
BpuEI CTTGAG 1 cut(s) 136
BpuMI CCSGG 1 cut(s) 854
BsaBI GATNNNNATC 3 cut(s) 423, 559, 774
BsaI GGTCTC 1 cut(s) 1005
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 449
Bse1I ACTGG 3 cut(s) 129, 197, 593
Bse8I GATNNNNATC 3 cut(s) 423, 559, 774
BseDI CCNNGG 1 cut(s) 30
BseJI GATNNNNATC 3 cut(s) 423, 559, 774
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 3 cut(s) 129, 197, 593
BseRI GAGGAG 4 cut(s) 287, 333, 342, 507
BseSI GKGCMC 2 cut(s) 124, 595
BseXI GCAGC 4 cut(s) 233, 635, 638, 641
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 1 cut(s) 857
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 2 cut(s) 124, 517
BsiSI CCGG 2 cut(s) 450, 854
BsmAI GTCTC 1 cut(s) 1005
BsmI GAATGC 1 cut(s) 942
BsnI GGCC 1 cut(s) 857
Bso31I GGTCTC 1 cut(s) 1005
Bsp1286I GDGCHC 3 cut(s) 124, 517, 595
Bsp143I GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
BspACI CCGC 4 cut(s) 318, 434, 541, 822
BspANI GGCC 1 cut(s) 857
BspCNI CTCAG 1 cut(s) 308
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 1 cut(s) 342
BspOI GCTAGC 1 cut(s) 828
BspPI GGATC 2 cut(s) 770, 834
BspQI GCTCTTC 2 cut(s) 297, 600
BspTNI GGTCTC 1 cut(s) 1005
BsrI ACTGG 3 cut(s) 129, 197, 593
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 181, 526
Bst6I CTCTTC 5 cut(s) 297, 464, 499, 600, 874
BstC8I GCNNGC 2 cut(s) 813, 826
BstDEI CTNAG 2 cut(s) 295, 912
BstFNI CGCG 1 cut(s) 434
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 749
BstKTI GATC 7 cut(s) 59, 375, 476, 548, 557, 778, 842
BstMAI GTCTC 1 cut(s) 1005
BstMBI GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 3 cut(s) 108, 629, 821
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 1 cut(s) 852
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 2 cut(s) 124, 595
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 4 cut(s) 233, 635, 638, 641
BstV2I GAAGAC 3 cut(s) 522, 824, 923
BstX2I RGATCY 3 cut(s) 545, 775, 839
BstYI RGATCY 3 cut(s) 545, 775, 839
BsuRI GGCC 1 cut(s) 857
Cac8I GCNNGC 2 cut(s) 813, 826
CfoI GCGC 2 cut(s) 53, 749
Cfr13I GGNCC 2 cut(s) 884, 1011
CviAII CATG 2 cut(s) 812, 896
DdeI CTNAG 2 cut(s) 295, 912
DpnI GATC 7 cut(s) 58, 374, 475, 547, 556, 777, 841
DpnII GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
DraI TTTAAA 1 cut(s) 336
Eam1104I CTCTTC 5 cut(s) 297, 464, 499, 600, 874
EarI CTCTTC 5 cut(s) 297, 464, 499, 600, 874
Ecl136II GAGCTC 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 517
Eco31I GGTCTC 1 cut(s) 1005
Eco47I GGWCC 2 cut(s) 884, 1011
Eco47III AGCGCT 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 515
Eco57I CTGAAG 3 cut(s) 321, 820, 873
EcoICRI GAGCTC 1 cut(s) 515
EcoO109I RGGNCCY 2 cut(s) 884, 1011
EcoRI GAATTC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 815, 899
FaiI YATR 6 cut(s) 14, 326, 424, 813, 845, 897
FalI AAGNNNNNCTT 8 cut(s) 871, 903, 905, 937, 908, 940, 943, 975
FatI CATG 2 cut(s) 811, 895
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 7 cut(s) 222, 319, 435, 541, 624, 627, 630
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 7 cut(s) 222, 319, 435, 541, 624, 627, 630
FspBI CTAG 3 cut(s) 825, 867, 947
GlaI GCGC 2 cut(s) 52, 748
GluI GCNGC 7 cut(s) 222, 319, 435, 541, 624, 627, 630
GsuI CTGGAG 1 cut(s) 814
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 857
HapII CCGG 2 cut(s) 450, 854
HhaI GCGC 2 cut(s) 53, 749
Hin1II CATG 2 cut(s) 815, 899
Hin6I GCGC 2 cut(s) 51, 747
HinP1I GCGC 2 cut(s) 51, 747
HinfI GANTC 2 cut(s) 419, 796
HpaII CCGG 2 cut(s) 450, 854
HphI GGTGA 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 122, 280
Hpy188I TCNGA 9 cut(s) 90, 148, 447, 580, 744, 754, 785, 801, 839
Hpy188III TCNNGA 7 cut(s) 115, 138, 552, 708, 793, 873, 1014
Hpy8I GTNNAC 2 cut(s) 122, 280
HpyAV CCTTC 4 cut(s) 90, 396, 897, 931
HpyCH4III ACNGT 2 cut(s) 181, 526
HpyCH4V TGCA 5 cut(s) 69, 122, 221, 699, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 629, 821
HpyF3I CTNAG 2 cut(s) 295, 912
Hsp92II CATG 2 cut(s) 815, 899
HspAI GCGC 2 cut(s) 51, 747
Kzo9I GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
LguI GCTCTTC 2 cut(s) 297, 600
LmnI GCTCC 4 cut(s) 346, 520, 744, 833
Lsp1109I GCAGC 4 cut(s) 233, 635, 638, 641
LweI GCATC 1 cut(s) 758
MaeI CTAG 3 cut(s) 825, 867, 947
MaeIII GTNAC 1 cut(s) 188
MalI GATC 7 cut(s) 58, 374, 475, 547, 556, 777, 841
MboI GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
MflI RGATCY 3 cut(s) 545, 775, 839
MhlI GDGCHC 3 cut(s) 124, 517, 595
MluCI AATT 5 cut(s) 161, 170, 356, 644, 666
MmeI TCCRAC 2 cut(s) 470, 545
MroXI GAANNNNTTC 2 cut(s) 95, 920
MseI TTAA 3 cut(s) 335, 585, 665
MspA1I CMGCKG 2 cut(s) 299, 479
MspI CCGG 2 cut(s) 450, 854
MspR9I CCNGG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 942
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 3 cut(s) 108, 629, 821
NciI CCSGG 1 cut(s) 854
NdeII GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
NheI GCTAGC 1 cut(s) 824
NlaIII CATG 2 cut(s) 815, 899
NlaIV GGNNCC 1 cut(s) 342
NspI RCATGY 1 cut(s) 815
PaeI GCATGC 1 cut(s) 815
PciSI GCTCTTC 2 cut(s) 297, 600
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 1 cut(s) 942
PdmI GAANNNNTTC 2 cut(s) 95, 920
PfeI GAWTC 2 cut(s) 419, 796
PkrI GCNGC 7 cut(s) 223, 320, 436, 542, 625, 628, 631
PpuMI RGGWCCY 2 cut(s) 884, 1011
PshBI ATTAAT 1 cut(s) 665
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 517
Psp5II RGGWCCY 2 cut(s) 884, 1011
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 2 cut(s) 884, 1011
PspPPI RGGWCCY 2 cut(s) 884, 1011
PsuI RGATCY 3 cut(s) 545, 775, 839
PvuII CAGCTG 2 cut(s) 299, 479
SacI GAGCTC 1 cut(s) 517
SapI GCTCTTC 2 cut(s) 297, 600
SaqAI TTAA 3 cut(s) 335, 585, 665
SatI GCNGC 7 cut(s) 222, 319, 435, 541, 624, 627, 630
Sau3AI GATC 7 cut(s) 56, 372, 473, 545, 554, 775, 839
Sau96I GGNCC 2 cut(s) 884, 1011
ScrFI CCNGG 1 cut(s) 854
SduI GDGCHC 3 cut(s) 124, 517, 595
SfaNI GCATC 1 cut(s) 758
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 2 cut(s) 884, 1011
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
SphI GCATGC 1 cut(s) 815
Sse9I AATT 5 cut(s) 161, 170, 356, 644, 666
SsiI CCGC 4 cut(s) 318, 434, 541, 822
SspI AATATT 1 cut(s) 379
SspMI CTAG 3 cut(s) 825, 867, 947
SstI GAGCTC 1 cut(s) 517
StyD4I CCNGG 1 cut(s) 852
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 181, 526
TaqI TCGA 4 cut(s) 39, 55, 139, 312
TasI AATT 5 cut(s) 161, 170, 356, 644, 666
TauI GCSGC 3 cut(s) 321, 437, 543
TfiI GAWTC 2 cut(s) 419, 796
Tru1I TTAA 3 cut(s) 335, 585, 665
Tru9I TTAA 3 cut(s) 335, 585, 665
TseI GCWGC 4 cut(s) 221, 623, 626, 629
TspDTI ATGAA 5 cut(s) 183, 411, 651, 660, 965
VneI GTGCAC 1 cut(s) 120
VpaK11BI GGWCC 2 cut(s) 884, 1011
VspI ATTAAT 1 cut(s) 665
XapI RAATTY 2 cut(s) 161, 170
XceI RCATGY 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 2 cut(s) 95, 920
XspI CTAG 3 cut(s) 825, 867, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.