MD15G1087600.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015304
Physical Location & Seq
Forward (+)
6105088 .. 6106146
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1087600.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGAGGTGCTGATACCAAGTAGAGCAGACATGGATTTCGACTTTAACAGCGCTCGATCCTCGCCGTCCGTGACTGCACCTTCGACACCACGGCGGTTTGGGGACTTCTACATGAGTGCACCAACAAGCCCAACTCGGATGTCCGAGTTTTATAGGGATTTTGAGGAGTTTTCCAACACGAGTAACTGGAAAGATGACGAGACAGCATCTCAAGCAGCCACCCCAAGATCACCGAAATCACATAAACGTGAGGAGGACTTTGGATTCGATGACTTTGCTTTTGATGTTGGTGAAGAGGTACAAAGAACTTCCCTCTCAGCTGAAGAGCTTTTCGATGGCGGCATCATAAGACCCTTTAATAAGCCTCCACCTACTCCATCCATTTTCCGTAAGGTTCTTTCGCCTAGACGGAAAAAGGGTAAAGAATATCCATTTGGGTTATCGTCCCCGACCCAAATGGACAACAACACAAGAAGAAGCGAGAGAAGAAGCGATCAACAACAACAAAGAGGTAGAGAGAGAGCTCCAGCAGCCTTGTCATCCTCCATTTCAGGCAGAAGAGCGACGAGATCGGTGTCACCTTTGAGGGTTTCGGAGTACCAATGGGTAGAAGAAGAAAAACAAAATCAACAGAAGCAACAGCAGCCACAACAAAACAACAAGCAGTTAGCTCCTACAGCTATGTTTACATCCTGCATAGTTTCTAAGGCTTCAAGAAAATGGAAACTGAAGGACTTTTTGTTGTTTCGAAGCGCATCGGAGGGAAGAGCCACAGATAAAGATCCGTTCAGGAAGTACTCAACTTTGTTCAGAAAAAATGAAGACATGAAGAACTCCAGCTTCAGGTCAATAGACAGCCCGGCCTCGGTTTCGAGCTCAAGGAGAAAAGGGCCGCCAGTTTCAGCTCACGAGTTGCATTACACTGTGAACAAAGCAGCGTCTAATGATATGAAGAAGAAAACCTTCTTGCCTTACAAGCAGGGCCTTTTGGGTAGATTGGCCTTCAATCCCGCCGTCAGTGCCCTTGCCAACGGCTTTGGGTCTCTTTCAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

353

Amino Acids

39.95

Weight (kDa)

10.01

Isoelectric Point (pI)

77.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 107 - 324 1.4e-44 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26530 AT2G26530
fragaria_vesca FvH4_2g34940
malus_domestica MD08G1106900.v1.1 MD15G1087600.v1.1
prunus_persica Prupe.1G441300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0307061
rosa_laevigata RLG00000010737
rosa_multiflora Rmu_sc0005634.1_g000005
rosa_roxburghii Rroxscaffold_7G00161190
rosa_rugosa Rorug06G0355600
rosa_samantha Rh6AG467100 Rh6BG433400 Rh6CG482600 Rh6DG468900
rosa_wichuraiana Rw6G040720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 94, 339, 893, 1011
AclWI GGATC 2 cut(s) 51, 776
AcuI CTGAAG 3 cut(s) 342, 749, 826
AfaI GTAC 3 cut(s) 300, 599, 797
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 843, 865
AgsI TTSAA 3 cut(s) 714, 1006, 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AluBI AGCT 8 cut(s) 320, 328, 524, 671, 680, 840, 876, 905
AluI AGCT 8 cut(s) 320, 328, 524, 671, 680, 840, 876, 905
Alw21I GWGCWC 3 cut(s) 121, 526, 878
Alw26I GTCTC 2 cut(s) 194, 1047
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 2 cut(s) 51, 776
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 4 cut(s) 861, 890, 982, 999
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 4 cut(s) 215, 530, 643, 935
ArsI GACNNNNNNTTYG 8 cut(s) 20, 52, 64, 96, 248, 263, 280, 295
Asp700I GAANNNNTTC 1 cut(s) 962
AspLEI GCGC 2 cut(s) 53, 755
AspS9I GGNCC 2 cut(s) 890, 982
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 3 cut(s) 222, 302, 570
AsuII TTCGAA 1 cut(s) 748
BaeGI GKGCMC 2 cut(s) 121, 1024
BanII GRGCYC 2 cut(s) 526, 878
BauI CACGAG 2 cut(s) 178, 908
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 3 cut(s) 121, 526, 878
BbvI GCAGC 4 cut(s) 227, 542, 655, 947
BccI CCATC 2 cut(s) 329, 385
BceAI ACGGC 4 cut(s) 49, 107, 998, 1048
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BcnI CCSGG 1 cut(s) 860
BcoDI GTCTC 2 cut(s) 194, 1047
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 6 cut(s) 216, 340, 531, 644, 893, 936
BlsI GCNGC 6 cut(s) 217, 341, 532, 645, 894, 937
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 1 cut(s) 860
BmgT120I GGNCC 2 cut(s) 890, 982
BmrFI CCNGG 1 cut(s) 860
BmsI GCATC 3 cut(s) 215, 351, 764
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 2 cut(s) 510, 820
Bpu14I TTCGAA 1 cut(s) 748
BpuEI CTTGAG 2 cut(s) 195, 862
BpuMI CCSGG 1 cut(s) 860
BsaBI GATNNNNATC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 2 cut(s) 89, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 843, 865
Bse1I ACTGG 2 cut(s) 191, 896
Bse8I GATNNNNATC 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 89, 864
BseGI GGATG 4 cut(s) 144, 377, 539, 689
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 843, 865
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 191, 896
BseRI GAGGAG 2 cut(s) 179, 266
BseSI GKGCMC 2 cut(s) 121, 1024
BseXI GCAGC 4 cut(s) 227, 542, 655, 947
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 4 cut(s) 863, 892, 984, 1001
BsiHKAI GWGCWC 3 cut(s) 121, 526, 878
BsiSI CCGG 1 cut(s) 860
BslFI GGGAC 2 cut(s) 116, 430
BslI CCNNNNNNNGG 2 cut(s) 843, 865
BsmAI GTCTC 2 cut(s) 194, 1047
BsmFI GGGAC 2 cut(s) 116, 430
BsnI GGCC 4 cut(s) 863, 892, 984, 1001
Bso31I GGTCTC 1 cut(s) 1047
Bsp119I TTCGAA 1 cut(s) 748
Bsp1286I GDGCHC 4 cut(s) 121, 526, 878, 1024
Bsp143I GATC 5 cut(s) 56, 227, 493, 569, 781
BspACI CCGC 4 cut(s) 94, 339, 893, 1011
BspANI GGCC 4 cut(s) 863, 892, 984, 1001
BspCNI CTCAG 1 cut(s) 329
BspPI GGATC 2 cut(s) 51, 776
BspQI GCTCTTC 3 cut(s) 318, 553, 760
BspT104I TTCGAA 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 1047
BsrI ACTGG 2 cut(s) 191, 896
BssECI CCNNGG 2 cut(s) 89, 864
BssMI GATC 5 cut(s) 56, 227, 493, 569, 781
BssSI CACGAG 2 cut(s) 178, 908
Bst2BI CACGAG 2 cut(s) 178, 908
Bst4CI ACNGT 1 cut(s) 925
Bst6I CTCTTC 4 cut(s) 288, 318, 553, 760
BstBI TTCGAA 1 cut(s) 748
BstDEI CTNAG 2 cut(s) 316, 705
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 4 cut(s) 144, 377, 539, 689
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 755
BstKTI GATC 5 cut(s) 59, 230, 496, 572, 784
BstMAI GTCTC 2 cut(s) 194, 1047
BstMBI GATC 5 cut(s) 56, 227, 493, 569, 781
BstMWI GCNNNNNNNGC 6 cut(s) 212, 530, 643, 677, 976, 1019
BstSCI CCNGG 1 cut(s) 858
BstSFI CTRYAG 1 cut(s) 675
BstSLI GKGCMC 2 cut(s) 121, 1024
BstV1I GCAGC 4 cut(s) 227, 542, 655, 947
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuRI GGCC 4 cut(s) 863, 892, 984, 1001
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 4 cut(s) 144, 377, 539, 689
BtsIMutI CAGTG 2 cut(s) 921, 1024
CfoI GCGC 2 cut(s) 53, 755
Cfr13I GGNCC 2 cut(s) 890, 982
CseI GACGC 1 cut(s) 927
Csp6I GTAC 3 cut(s) 299, 598, 796
CviAII CATG 3 cut(s) 31, 112, 826
CviQI GTAC 3 cut(s) 299, 598, 796
DdeI CTNAG 2 cut(s) 316, 705
DpnI GATC 5 cut(s) 58, 229, 495, 571, 783
DpnII GATC 5 cut(s) 56, 227, 493, 569, 781
Eam1104I CTCTTC 4 cut(s) 288, 318, 553, 760
EarI CTCTTC 4 cut(s) 288, 318, 553, 760
Ecl136II GAGCTC 2 cut(s) 524, 876
Eco24I GRGCYC 2 cut(s) 526, 878
Eco31I GGTCTC 1 cut(s) 1047
Eco47III AGCGCT 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 524, 876
Eco57I CTGAAG 3 cut(s) 342, 749, 826
EcoICRI GAGCTC 2 cut(s) 524, 876
EcoO109I RGGNCCY 1 cut(s) 982
EcoT38I GRGCYC 2 cut(s) 526, 878
FaeI CATG 3 cut(s) 34, 115, 829
FaiI YATR 9 cut(s) 32, 113, 153, 243, 347, 683, 698, 827, 950
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FaqI GGGAC 2 cut(s) 116, 430
FatI CATG 3 cut(s) 30, 111, 825
FauI CCCGC 1 cut(s) 1018
Fnu4HI GCNGC 6 cut(s) 216, 340, 531, 644, 893, 936
FokI GGATG 4 cut(s) 151, 364, 526, 676
FriOI GRGCYC 2 cut(s) 526, 878
Fsp4HI GCNGC 6 cut(s) 216, 340, 531, 644, 893, 936
FspBI CTAG 1 cut(s) 405
GlaI GCGC 2 cut(s) 52, 754
GluI GCNGC 6 cut(s) 216, 340, 531, 644, 893, 936
GsuI CTGGAG 2 cut(s) 510, 820
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 4 cut(s) 863, 892, 984, 1001
HapII CCGG 1 cut(s) 860
HgaI GACGC 1 cut(s) 927
HhaI GCGC 2 cut(s) 53, 755
Hin1II CATG 3 cut(s) 34, 115, 829
Hin6I GCGC 2 cut(s) 51, 753
HinP1I GCGC 2 cut(s) 51, 753
HinfI GANTC 1 cut(s) 264
HpaII CCGG 1 cut(s) 860
HphI GGTGA 3 cut(s) 222, 302, 570
Hpy166II GTNNAC 3 cut(s) 119, 687, 928
Hpy188I TCNGA 5 cut(s) 138, 145, 595, 760, 812
Hpy188III TCNNGA 4 cut(s) 714, 790, 908, 1050
Hpy8I GTNNAC 3 cut(s) 119, 687, 928
Hpy99I CGWCG 1 cut(s) 568
HpyAV CCTTC 4 cut(s) 90, 724, 973, 1012
HpyCH4III ACNGT 1 cut(s) 925
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 4 cut(s) 77, 119, 696, 916
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 530, 643, 677, 976, 1019
HpyF3I CTNAG 2 cut(s) 316, 705
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 3 cut(s) 34, 115, 829
HspAI GCGC 2 cut(s) 51, 753
Kzo9I GATC 5 cut(s) 56, 227, 493, 569, 781
LguI GCTCTTC 3 cut(s) 318, 553, 760
LmnI GCTCC 2 cut(s) 529, 676
Lsp1109I GCAGC 4 cut(s) 227, 542, 655, 947
LweI GCATC 3 cut(s) 215, 351, 764
MaeI CTAG 1 cut(s) 405
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 3 cut(s) 70, 182, 576
MalI GATC 5 cut(s) 58, 229, 495, 571, 783
MboI GATC 5 cut(s) 56, 227, 493, 569, 781
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 4 cut(s) 121, 526, 878, 1024
MmeI TCCRAC 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 962
MseI TTAA 2 cut(s) 45, 357
MslI CAYNNNNRTG 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 860
MspR9I CCNGG 1 cut(s) 860
MwoI GCNNNNNNNGC 6 cut(s) 212, 530, 643, 677, 976, 1019
NciI CCSGG 1 cut(s) 860
NdeII GATC 5 cut(s) 56, 227, 493, 569, 781
NlaIII CATG 3 cut(s) 34, 115, 829
NmuCI GTSAC 2 cut(s) 70, 576
NspV TTCGAA 1 cut(s) 748
PciSI GCTCTTC 3 cut(s) 318, 553, 760
PdmI GAANNNNTTC 1 cut(s) 962
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 6 cut(s) 217, 341, 532, 645, 894, 937
Psp124BI GAGCTC 2 cut(s) 526, 878
PspPI GGNCC 2 cut(s) 890, 982
PsuI RGATCY 1 cut(s) 781
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 3 cut(s) 300, 599, 797
RsaNI GTAC 3 cut(s) 299, 598, 796
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 2 cut(s) 526, 878
SapI GCTCTTC 3 cut(s) 318, 553, 760
SaqAI TTAA 2 cut(s) 45, 357
SatI GCNGC 6 cut(s) 216, 340, 531, 644, 893, 936
Sau3AI GATC 5 cut(s) 56, 227, 493, 569, 781
Sau96I GGNCC 2 cut(s) 890, 982
ScaI AGTACT 1 cut(s) 797
ScrFI CCNGG 1 cut(s) 860
SduI GDGCHC 4 cut(s) 121, 526, 878, 1024
SfaNI GCATC 3 cut(s) 215, 351, 764
SfcI CTRYAG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 2 cut(s) 210, 877
SmoI CTYRAG 2 cut(s) 210, 877
SsiI CCGC 4 cut(s) 94, 339, 893, 1011
SspMI CTAG 1 cut(s) 405
SstI GAGCTC 2 cut(s) 526, 878
StyD4I CCNGG 1 cut(s) 858
TaaI ACNGT 1 cut(s) 925
TaiI ACGT 1 cut(s) 250
TaqI TCGA 7 cut(s) 39, 55, 83, 267, 333, 748, 872
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 342, 895
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 2 cut(s) 45, 357
Tru9I TTAA 2 cut(s) 45, 357
TscAI CASTG 2 cut(s) 928, 1024
TseFI GTSAC 2 cut(s) 70, 576
TseI GCWGC 4 cut(s) 215, 530, 643, 935
Tsp45I GTSAC 2 cut(s) 70, 576
TspDTI ATGAA 3 cut(s) 834, 842, 965
TspGWI ACGGA 4 cut(s) 58, 377, 424, 774
TspRI CASTG 2 cut(s) 928, 1024
VneI GTGCAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 962
XspI CTAG 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.