MD08G1106900.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015304
Physical Location & Seq
Forward (+)
9387087 .. 9388921
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1106900.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGAGGTGATAATACCAAGTAGAGCAGCCATGGATTTCGACTTCAACAGCGCTCGATCCTCGCAGTCTGTGACTGAGCCTTCGACACCAAGGCGGTTTGGGGACTTCTACATGAGTGCACCAACAAGCCCAACTCGGATGTCCGAGTTTTATAGGGATTTTGAGGAGTTTTCCAACATGAGTAACTGGGAAGATGATGAGGCAGCATCTCAAGCAGCCACGCCAAAATCGCCTAAATCGCCTAAACGTGAGGATGACTTTGGATTCGATGACTTTGCTTTTGATGTTGGTGAAGATGTACAAAGAACTTCTCTTTCAGCTGAAGAGCTTTTCGATGGCGGCATCATTAGACCCTTTAATAAGCCTCCACCCACTCCATCATTTTTCCGTCAGGTTCTGTCTCCTAGACGGAAAAAGGGTAAAGAATATCCGAAGACCCAAATGGACAACACAACAAGAAGAAGCGAGAGAATAAGCGAACAACAACAAAGAGGTAGAGAGAGAACTCCAGCAGCCTTGTCATCCTCCCTTTCAGGCCGAAGAGCGACAAGATCGGTATCACCTTTGAGGGTCTCCGAGTACCAATGGGTAGAAGAAGAAAAACAAAACCAACAGCAGCAAAGGAAGCAACAACAAAACAACAAGCAGTTAACTCCCAAAGCTATGTTTACATCCTGCACAGATTCTAAGGCTTCAAGAAAATGGAAACTAAAGGATTTTTTGTTGTTTCGAAGCGCCTCGGAGGGAAGAGCCACAGATAAAGATCCATTCACGAAGTACTTAACTTTGTTTAGGAAAAATGAAGACGTGAAGAACTCCAGCTTCAGGTCAATAGACAGCCCGGCCTCAGTTTCGATCTCGAGGAGAAGAGGGCCGCCAGTTTCAGCTCACGAGTTGCATTACACTGCGAACAAAGCAGTGTCTAATGATATGAAGAAGAAGACCTTCTTGCCTTACAAGCAGGGCATTTTGGGCAGATTGGCCTTCAATCCCGCGGTCAGTGCCCTTGCCAATGGCTTTGGGTCTCTTTCAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

347

Amino Acids

39.44

Weight (kDa)

9.86

Isoelectric Point (pI)

76.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 107 - 318 1.3e-45 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26530 AT2G26530
fragaria_vesca FvH4_2g34940
malus_domestica MD08G1106900.v1.1 MD15G1087600.v1.1
prunus_persica Prupe.1G441300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0307061
rosa_laevigata RLG00000010737
rosa_multiflora Rmu_sc0005634.1_g000005
rosa_roxburghii Rroxscaffold_7G00161190
rosa_rugosa Rorug06G0355600
rosa_samantha Rh6AG467100 Rh6BG433400 Rh6CG482600 Rh6DG468900
rosa_wichuraiana Rw6G040720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 995
AciI CCGC 5 cut(s) 94, 339, 875, 993, 995
AclWI GGATC 2 cut(s) 51, 758
AcuI CTGAAG 2 cut(s) 342, 808
AfaI GTAC 3 cut(s) 300, 581, 779
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 1 cut(s) 825
AgsI TTSAA 4 cut(s) 46, 696, 988, 1032
AjiI CACGTC 1 cut(s) 808
AluBI AGCT 5 cut(s) 320, 328, 662, 822, 887
AluI AGCT 5 cut(s) 320, 328, 662, 822, 887
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 3 cut(s) 405, 577, 1029
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 2 cut(s) 51, 758
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 859
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 4 cut(s) 535, 843, 872, 981
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 5 cut(s) 26, 203, 215, 512, 616
ArsI GACNNNNNNTTYG 6 cut(s) 64, 96, 248, 263, 280, 295
Asp700I GAANNNNTTC 1 cut(s) 944
AspLEI GCGC 2 cut(s) 53, 737
AspS9I GGNCC 1 cut(s) 872
AsuC2I CCSGG 1 cut(s) 842
AsuHPI GGTGA 3 cut(s) 19, 302, 552
AsuII TTCGAA 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 859
BaeGI GKGCMC 2 cut(s) 121, 1006
BauI CACGAG 1 cut(s) 890
BbsI GAAGAC 3 cut(s) 440, 810, 947
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 5 cut(s) 38, 215, 227, 524, 628
BccI CCATC 2 cut(s) 329, 385
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BcnI CCSGG 1 cut(s) 842
BcoDI GTCTC 3 cut(s) 405, 577, 1029
BfaI CTAG 1 cut(s) 405
BfoI RGCGCY 2 cut(s) 54, 738
BisI GCNGC 7 cut(s) 27, 204, 216, 340, 513, 617, 875
BlsI GCNGC 7 cut(s) 28, 205, 217, 341, 514, 618, 876
BmcAI AGTACT 1 cut(s) 779
Bme1390I CCNGG 1 cut(s) 842
BmeT110I CYCGRG 1 cut(s) 859
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 872
BmrFI CCNGG 1 cut(s) 842
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 2 cut(s) 215, 351
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 440, 810, 947
BpmI CTGGAG 2 cut(s) 492, 802
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 842
BsaBI GATNNNNATC 2 cut(s) 556, 762
BsaI GGTCTC 2 cut(s) 577, 1029
BsaJI CCNNGG 4 cut(s) 30, 89, 738, 993
Bsc4I CCNNNNNNNGG 1 cut(s) 825
Bse1I ACTGG 2 cut(s) 191, 878
Bse8I GATNNNNATC 2 cut(s) 556, 762
BseDI CCNNGG 4 cut(s) 30, 89, 738, 993
BseGI GGATG 4 cut(s) 144, 259, 521, 671
BseJI GATNNNNATC 2 cut(s) 556, 762
BseLI CCNNNNNNNGG 1 cut(s) 825
BseMII CTCAG 2 cut(s) 66, 861
BseNI ACTGG 2 cut(s) 191, 878
BseRI GAGGAG 2 cut(s) 179, 877
BseSI GKGCMC 2 cut(s) 121, 1006
BseXI GCAGC 5 cut(s) 38, 215, 227, 524, 628
BsgI GTGCAG 1 cut(s) 661
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 4 cut(s) 537, 845, 874, 983
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 859
BsiSI CCGG 1 cut(s) 842
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 825
BsmAI GTCTC 3 cut(s) 405, 577, 1029
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 4 cut(s) 537, 845, 874, 983
Bso31I GGTCTC 2 cut(s) 577, 1029
BsoBI CYCGRG 1 cut(s) 859
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 2 cut(s) 121, 1006
Bsp1407I TGTACA 1 cut(s) 298
Bsp143I GATC 4 cut(s) 56, 551, 763, 855
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 5 cut(s) 94, 339, 875, 993, 995
BspANI GGCC 4 cut(s) 537, 845, 874, 983
BspCNI CTCAG 2 cut(s) 67, 860
BspFNI CGCG 1 cut(s) 995
BspPI GGATC 2 cut(s) 51, 758
BspQI GCTCTTC 3 cut(s) 318, 535, 742
BspT104I TTCGAA 1 cut(s) 730
BspTNI GGTCTC 2 cut(s) 577, 1029
BsrGI TGTACA 1 cut(s) 298
BsrI ACTGG 2 cut(s) 191, 878
BssECI CCNNGG 4 cut(s) 30, 89, 738, 993
BssMI GATC 4 cut(s) 56, 551, 763, 855
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 2 cut(s) 30, 89
Bst2BI CACGAG 1 cut(s) 890
Bst6I CTCTTC 4 cut(s) 318, 535, 742, 862
BstAUI TGTACA 1 cut(s) 298
BstBI TTCGAA 1 cut(s) 730
BstDEI CTNAG 3 cut(s) 75, 687, 847
BstDSI CCRYGG 2 cut(s) 30, 993
BstF5I GGATG 4 cut(s) 144, 259, 521, 671
BstFNI CGCG 1 cut(s) 995
BstH2I RGCGCY 2 cut(s) 54, 738
BstHHI GCGC 2 cut(s) 53, 737
BstKTI GATC 4 cut(s) 59, 554, 766, 858
BstMAI GTCTC 3 cut(s) 405, 577, 1029
BstMBI GATC 4 cut(s) 56, 551, 763, 855
BstMWI GCNNNNNNNGC 8 cut(s) 212, 229, 238, 625, 914, 958, 972, 1001
BstSCI CCNGG 1 cut(s) 840
BstSLI GKGCMC 2 cut(s) 121, 1006
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 5 cut(s) 38, 215, 227, 524, 628
BstV2I GAAGAC 3 cut(s) 440, 810, 947
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
BsuRI GGCC 4 cut(s) 537, 845, 874, 983
BtgI CCRYGG 2 cut(s) 30, 993
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 4 cut(s) 144, 259, 521, 671
BtsI GCAGTG 2 cut(s) 903, 924
BtsIMutI CAGTG 3 cut(s) 903, 924, 1006
CaiI CAGNNNCTG 1 cut(s) 397
CfoI GCGC 2 cut(s) 53, 737
Cfr13I GGNCC 1 cut(s) 872
Cfr42I CCGCGG 1 cut(s) 996
Csp6I GTAC 3 cut(s) 299, 580, 778
CviAII CATG 3 cut(s) 31, 112, 178
CviQI GTAC 3 cut(s) 299, 580, 778
DdeI CTNAG 3 cut(s) 75, 687, 847
DpnI GATC 4 cut(s) 58, 553, 765, 857
DpnII GATC 4 cut(s) 56, 551, 763, 855
Eam1104I CTCTTC 4 cut(s) 318, 535, 742, 862
EarI CTCTTC 4 cut(s) 318, 535, 742, 862
Eco130I CCWWGG 2 cut(s) 30, 89
Eco31I GGTCTC 2 cut(s) 577, 1029
Eco47III AGCGCT 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 342, 808
Eco88I CYCGRG 1 cut(s) 859
EcoT14I CCWWGG 2 cut(s) 30, 89
ErhI CCWWGG 2 cut(s) 30, 89
FaeI CATG 3 cut(s) 34, 115, 181
FaiI YATR 6 cut(s) 32, 113, 153, 179, 665, 932
FalI AAGNNNNNCTT 4 cut(s) 929, 961, 932, 964
FaqI GGGAC 1 cut(s) 116
FatI CATG 3 cut(s) 30, 111, 177
FauI CCCGC 1 cut(s) 1000
Fnu4HI GCNGC 7 cut(s) 27, 204, 216, 340, 513, 617, 875
FokI GGATG 4 cut(s) 151, 266, 508, 658
Fsp4HI GCNGC 7 cut(s) 27, 204, 216, 340, 513, 617, 875
FspBI CTAG 1 cut(s) 405
GlaI GCGC 2 cut(s) 52, 736
GluI GCNGC 7 cut(s) 27, 204, 216, 340, 513, 617, 875
GsuI CTGGAG 2 cut(s) 492, 802
HaeII RGCGCY 2 cut(s) 54, 738
HaeIII GGCC 4 cut(s) 537, 845, 874, 983
HapII CCGG 1 cut(s) 842
HhaI GCGC 2 cut(s) 53, 737
Hin1II CATG 3 cut(s) 34, 115, 181
Hin6I GCGC 2 cut(s) 51, 735
HinP1I GCGC 2 cut(s) 51, 735
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HinfI GANTC 2 cut(s) 264, 683
HpaI GTTAAC 1 cut(s) 651
HpaII CCGG 1 cut(s) 842
HphI GGTGA 3 cut(s) 19, 302, 552
Hpy166II GTNNAC 3 cut(s) 119, 651, 669
Hpy188I TCNGA 5 cut(s) 138, 145, 432, 577, 742
Hpy188III TCNNGA 5 cut(s) 696, 772, 859, 890, 1032
Hpy8I GTNNAC 3 cut(s) 119, 651, 669
HpyAV CCTTC 3 cut(s) 90, 955, 994
HpyCH4IV ACGT 2 cut(s) 247, 807
HpyCH4V TGCA 3 cut(s) 119, 678, 898
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 229, 238, 625, 914, 958, 972, 1001
HpyF3I CTNAG 3 cut(s) 75, 687, 847
HpySE526I ACGT 2 cut(s) 247, 807
Hsp92II CATG 3 cut(s) 34, 115, 181
HspAI GCGC 2 cut(s) 51, 735
KspAI GTTAAC 1 cut(s) 651
KspI CCGCGG 1 cut(s) 996
Kzo9I GATC 4 cut(s) 56, 551, 763, 855
LguI GCTCTTC 3 cut(s) 318, 535, 742
Lsp1109I GCAGC 5 cut(s) 38, 215, 227, 524, 628
LweI GCATC 2 cut(s) 215, 351
MaeI CTAG 1 cut(s) 405
MaeII ACGT 2 cut(s) 247, 807
MaeIII GTNAC 2 cut(s) 70, 182
MalI GATC 4 cut(s) 58, 553, 765, 857
MboI GATC 4 cut(s) 56, 551, 763, 855
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 2 cut(s) 121, 1006
MmeI TCCRAC 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 944
MseI TTAA 3 cut(s) 357, 650, 782
MspA1I CMGCKG 2 cut(s) 320, 995
MspI CCGG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 842
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 8 cut(s) 212, 229, 238, 625, 914, 958, 972, 1001
NciI CCSGG 1 cut(s) 842
NcoI CCATGG 1 cut(s) 30
NdeII GATC 4 cut(s) 56, 551, 763, 855
NlaIII CATG 3 cut(s) 34, 115, 181
NmuCI GTSAC 1 cut(s) 70
NspV TTCGAA 1 cut(s) 730
PaeR7I CTCGAG 1 cut(s) 859
PciSI GCTCTTC 3 cut(s) 318, 535, 742
PdmI GAANNNNTTC 1 cut(s) 944
PfeI GAWTC 2 cut(s) 264, 683
PkrI GCNGC 7 cut(s) 28, 205, 217, 341, 514, 618, 876
PspPI GGNCC 1 cut(s) 872
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 3 cut(s) 300, 581, 779
RsaNI GTAC 3 cut(s) 299, 580, 778
SacII CCGCGG 1 cut(s) 996
SapI GCTCTTC 3 cut(s) 318, 535, 742
SaqAI TTAA 3 cut(s) 357, 650, 782
SatI GCNGC 7 cut(s) 27, 204, 216, 340, 513, 617, 875
Sau3AI GATC 4 cut(s) 56, 551, 763, 855
Sau96I GGNCC 1 cut(s) 872
ScaI AGTACT 1 cut(s) 779
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 2 cut(s) 121, 1006
SfaNI GCATC 2 cut(s) 215, 351
Sfr274I CTCGAG 1 cut(s) 859
Sfr303I CCGCGG 1 cut(s) 996
SfuI TTCGAA 1 cut(s) 730
SgrBI CCGCGG 1 cut(s) 996
SlaI CTCGAG 1 cut(s) 859
SmlI CTYRAG 2 cut(s) 210, 859
SmoI CTYRAG 2 cut(s) 210, 859
SsiI CCGC 5 cut(s) 94, 339, 875, 993, 995
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 840
StyI CCWWGG 2 cut(s) 30, 89
TaiI ACGT 2 cut(s) 250, 810
TaqI TCGA 8 cut(s) 39, 55, 83, 267, 333, 730, 854, 860
TatI WGTACW 2 cut(s) 298, 777
TauI GCSGC 2 cut(s) 342, 877
TfiI GAWTC 2 cut(s) 264, 683
Tru1I TTAA 3 cut(s) 357, 650, 782
Tru9I TTAA 3 cut(s) 357, 650, 782
TscAI CASTG 3 cut(s) 910, 924, 1006
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 5 cut(s) 26, 203, 215, 512, 616
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 2 cut(s) 816, 947
TspGWI ACGGA 2 cut(s) 377, 424
TspRI CASTG 3 cut(s) 910, 924, 1006
VneI GTGCAC 1 cut(s) 117
XhoI CTCGAG 1 cut(s) 859
XmnI GAANNNNTTC 1 cut(s) 944
XspI CTAG 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.