RLG00000010737

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015304
Physical Location & Seq
Forward (+)
4027436 .. 4029042
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010737

Sequence Viewer

Length: 1050 bp
ATGGAGGTAGCTATACCAAGCAGACCCACCTTGGATTTCGACTTCAACAGCGCTCGATCTTCGCCTTGCATTACTGCTCCTTCAACTCCGAGACGCTTCGGCGACTACAGCTTCCTGAGTGCACCAACCAGTCCATCTCGAATGTCCGAGTTTTACAGAGAATTTGATGAATTCTCCACAGTCAGTGGTAGCTGGGAAGAAGAAGATGAGCCTGAAGCTGCAGCACCACCCAAATCACCAAAACCAGACGACTTTGCGTTTGATGTTAGCGAGGAGGTAGACAGAGCTTCTCTCTCAGCTGAAGAGCTTTTCGATGGCGGCGTTATAAGACCTTTAAAGGCTCCTCCATCGCCTCAATTCTCAACAAAGAAGATCAATATTCGTCAAGCTCTTTCGCCCAGAAGGAGAAAAGATAGAGATTCATATCCATTCGCGGAGAACAATTCGACCGGAATGGAAACTCCCCAGAAGAGATCAGCTGTTTCTTCACAGCAACAAAGAGGAAGAGAAAGAGCTCCTCCAACCGTGTCTTCTTCAAGCCGCAGATCTTCAAGATCACTATCTCCTTTGAGGGTTTCAGAGTTTAACTGGGCACAAGAAGAAGAAGAGCTAAAACGACAAGCAGCAGCAGCAGCACAGAACAAGCAATTAGCTACAAAGGGTTCATTAATTTCATCTGCTACTGCTGCTTTGAATAGTTGCAAGGCTTCAAGAAAATGGAGGCTGAAAGATTTTCTGTTGTTTCGGAGCGCATCAGAGGGCAGAGCAACAGATAAAGATCCATTCAGAAAGTTCTGGAATCAGAAAAACGAGCATGCGAAGACCGCTAGCTCCAGCTTCAGATCCATAGACAGCCCGGCCTCTGTTTCGAGTTCAAGAAGAAGAGGACCTTCAGCACATGAGTTGCATTACACTGTGAACAAGGCAGTGTCCGATGATATGAAGAAGAAAACCTTCTTGCCTTACAAACAGGGCATTCTAGGAAGATTGGCTTTCAATCCTGCTGTTCATTCTCTTGCCAATGGATTTGGGTCTCTTTCAAGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

350

Amino Acids

38.79

Weight (kDa)

9.78

Isoelectric Point (pI)

80.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 100 - 321 3.6e-40 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26530 AT2G26530
fragaria_vesca FvH4_2g34940
malus_domestica MD08G1106900.v1.1 MD15G1087600.v1.1
prunus_persica Prupe.1G441300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0307061
rosa_laevigata RLG00000010737
rosa_multiflora Rmu_sc0005634.1_g000005
rosa_roxburghii Rroxscaffold_7G00161190
rosa_rugosa Rorug06G0355600
rosa_samantha Rh6AG467100 Rh6BG433400 Rh6CG482600 Rh6DG468900
rosa_wichuraiana Rw6G040720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 434
AciI CCGC 4 cut(s) 318, 434, 541, 825
AclWI GGATC 2 cut(s) 773, 837
AcsI RAATTY 2 cut(s) 161, 170
AcuI CTGAAG 4 cut(s) 234, 321, 823, 876
AfeI AGCGCT 1 cut(s) 52
AgsI TTSAA 9 cut(s) 46, 84, 537, 552, 694, 711, 876, 997, 1041
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
Alw21I GWGCWC 2 cut(s) 124, 517
Alw26I GTCTC 2 cut(s) 85, 1038
Alw44I GTGCAC 1 cut(s) 120
AlwI GGATC 2 cut(s) 773, 837
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 1 cut(s) 858
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 7 cut(s) 218, 221, 623, 626, 629, 632, 686
ApoI RAATTY 2 cut(s) 161, 170
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 953
AspLEI GCGC 2 cut(s) 53, 752
AspS9I GGNCC 2 cut(s) 887, 1044
AsuC2I CCSGG 1 cut(s) 857
AsuHPI GGTGA 1 cut(s) 228
AsuNHI GCTAGC 1 cut(s) 827
AvaII GGWCC 2 cut(s) 887, 1044
BaeGI GKGCMC 2 cut(s) 124, 595
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 2 cut(s) 522, 827
Bbv12I GWGCWC 2 cut(s) 124, 517
BbvI GCAGC 7 cut(s) 205, 233, 635, 638, 641, 644, 673
BccI CCATC 3 cut(s) 142, 308, 355
BcnI CCSGG 1 cut(s) 857
BcoDI GTCTC 2 cut(s) 85, 1038
BfaI CTAG 2 cut(s) 828, 980
BfmI CTRYAG 2 cut(s) 106, 219
BfoI RGCGCY 1 cut(s) 54
BglII AGATCT 1 cut(s) 545
BisI GCNGC 9 cut(s) 219, 222, 319, 541, 624, 627, 630, 633, 687
BlsI GCNGC 9 cut(s) 220, 223, 320, 542, 625, 628, 631, 634, 688
Bme1390I CCNGG 1 cut(s) 857
Bme18I GGWCC 2 cut(s) 887, 1044
BmgT120I GGNCC 2 cut(s) 887, 1044
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 857
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 1 cut(s) 761
BmtI GCTAGC 1 cut(s) 831
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 2 cut(s) 522, 827
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpmI CTGGAG 1 cut(s) 817
BpuMI CCSGG 1 cut(s) 857
BsaBI GATNNNNATC 3 cut(s) 423, 559, 777
BsaI GGTCTC 1 cut(s) 1038
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 129, 593
Bse8I GATNNNNATC 3 cut(s) 423, 559, 777
BseDI CCNNGG 1 cut(s) 30
BseJI GATNNNNATC 3 cut(s) 423, 559, 777
BseMII CTCAG 2 cut(s) 107, 309
BseNI ACTGG 2 cut(s) 129, 593
BseRI GAGGAG 3 cut(s) 287, 333, 507
BseSI GKGCMC 2 cut(s) 124, 595
BseXI GCAGC 7 cut(s) 205, 233, 635, 638, 641, 644, 673
BseYI CCCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 1 cut(s) 860
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 2 cut(s) 124, 517
BsiSI CCGG 2 cut(s) 450, 857
BsmAI GTCTC 2 cut(s) 85, 1038
BsmBI CGTCTC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 1 cut(s) 860
Bso31I GGTCTC 1 cut(s) 1038
Bsp1286I GDGCHC 3 cut(s) 124, 517, 595
Bsp143I GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
BspACI CCGC 4 cut(s) 318, 434, 541, 825
BspANI GGCC 1 cut(s) 860
BspCNI CTCAG 2 cut(s) 108, 308
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 1 cut(s) 342
BspMAI CTGCAG 1 cut(s) 223
BspOI GCTAGC 1 cut(s) 831
BspPI GGATC 2 cut(s) 773, 837
BspQI GCTCTTC 2 cut(s) 297, 600
BspTNI GGTCTC 1 cut(s) 1038
BsrI ACTGG 2 cut(s) 129, 593
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 3 cut(s) 181, 526, 916
Bst6I CTCTTC 5 cut(s) 297, 464, 499, 600, 877
BstC8I GCNNGC 2 cut(s) 816, 829
BstDEI CTNAG 2 cut(s) 116, 295
BstFNI CGCG 1 cut(s) 434
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 752
BstKTI GATC 7 cut(s) 59, 375, 476, 548, 557, 781, 845
BstMAI GTCTC 2 cut(s) 85, 1038
BstMBI GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 5 cut(s) 108, 629, 632, 686, 824
BstNSI RCATGY 1 cut(s) 818
BstSCI CCNGG 1 cut(s) 855
BstSFI CTRYAG 2 cut(s) 106, 219
BstSLI GKGCMC 2 cut(s) 124, 595
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 7 cut(s) 205, 233, 635, 638, 641, 644, 673
BstV2I GAAGAC 2 cut(s) 522, 827
BstX2I RGATCY 3 cut(s) 545, 778, 842
BstYI RGATCY 3 cut(s) 545, 778, 842
BsuRI GGCC 1 cut(s) 860
BtgZI GCGATG 1 cut(s) 333
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 3 cut(s) 190, 912, 933
Cac8I GCNNGC 2 cut(s) 816, 829
CfoI GCGC 2 cut(s) 53, 752
Cfr13I GGNCC 2 cut(s) 887, 1044
CseI GACGC 1 cut(s) 102
CviAII CATG 2 cut(s) 815, 899
DdeI CTNAG 2 cut(s) 116, 295
DpnI GATC 7 cut(s) 58, 374, 475, 547, 556, 780, 844
DpnII GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
DraI TTTAAA 1 cut(s) 336
Eam1104I CTCTTC 5 cut(s) 297, 464, 499, 600, 877
EarI CTCTTC 5 cut(s) 297, 464, 499, 600, 877
Ecl136II GAGCTC 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 517
Eco31I GGTCTC 1 cut(s) 1038
Eco47I GGWCC 2 cut(s) 887, 1044
Eco47III AGCGCT 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 515
Eco57I CTGAAG 4 cut(s) 234, 321, 823, 876
EcoICRI GAGCTC 1 cut(s) 515
EcoO109I RGGNCCY 2 cut(s) 887, 1044
EcoRI GAATTC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 2 cut(s) 818, 902
FaiI YATR 7 cut(s) 14, 326, 424, 816, 848, 900, 941
FalI AAGNNNNNCTT 6 cut(s) 874, 906, 938, 970, 976, 1008
FatI CATG 2 cut(s) 814, 898
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 9 cut(s) 219, 222, 319, 541, 624, 627, 630, 633, 687
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 9 cut(s) 219, 222, 319, 541, 624, 627, 630, 633, 687
FspBI CTAG 2 cut(s) 828, 980
GlaI GCGC 2 cut(s) 52, 751
GluI GCNGC 9 cut(s) 219, 222, 319, 541, 624, 627, 630, 633, 687
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 1 cut(s) 817
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 860
HapII CCGG 2 cut(s) 450, 857
HgaI GACGC 1 cut(s) 102
HhaI GCGC 2 cut(s) 53, 752
Hin1II CATG 2 cut(s) 818, 902
Hin6I GCGC 2 cut(s) 51, 750
HinP1I GCGC 2 cut(s) 51, 750
HinfI GANTC 2 cut(s) 419, 799
HpaII CCGG 2 cut(s) 450, 857
HphI GGTGA 1 cut(s) 228
Hpy166II GTNNAC 3 cut(s) 122, 280, 919
Hpy188I TCNGA 9 cut(s) 90, 148, 580, 747, 757, 788, 804, 842, 934
Hpy188III TCNNGA 7 cut(s) 115, 138, 552, 711, 796, 876, 1047
Hpy8I GTNNAC 3 cut(s) 122, 280, 919
HpyAV CCTTC 4 cut(s) 90, 396, 900, 964
HpyCH4III ACNGT 3 cut(s) 181, 526, 916
HpyCH4V TGCA 5 cut(s) 69, 122, 221, 702, 907
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 629, 632, 686, 824
HpyF3I CTNAG 2 cut(s) 116, 295
Hsp92II CATG 2 cut(s) 818, 902
HspAI GCGC 2 cut(s) 51, 750
Kzo9I GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
LguI GCTCTTC 2 cut(s) 297, 600
LmnI GCTCC 5 cut(s) 82, 346, 520, 747, 836
Lsp1109I GCAGC 7 cut(s) 205, 233, 635, 638, 641, 644, 673
LweI GCATC 1 cut(s) 761
MaeI CTAG 2 cut(s) 828, 980
MalI GATC 7 cut(s) 58, 374, 475, 547, 556, 780, 844
MboI GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
MflI RGATCY 3 cut(s) 545, 778, 842
MhlI GDGCHC 3 cut(s) 124, 517, 595
MluCI AATT 6 cut(s) 161, 170, 356, 442, 647, 669
MmeI TCCRAC 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 953
MseI TTAA 3 cut(s) 335, 585, 668
MspA1I CMGCKG 2 cut(s) 299, 479
MspI CCGG 2 cut(s) 450, 857
MspR9I CCNGG 1 cut(s) 857
Mva1269I GAATGC 1 cut(s) 975
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 5 cut(s) 108, 629, 632, 686, 824
NciI CCSGG 1 cut(s) 857
NdeII GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
NheI GCTAGC 1 cut(s) 827
NlaIII CATG 2 cut(s) 818, 902
NlaIV GGNNCC 1 cut(s) 342
NspI RCATGY 1 cut(s) 818
PaeI GCATGC 1 cut(s) 818
PciSI GCTCTTC 2 cut(s) 297, 600
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 1 cut(s) 975
PdmI GAANNNNTTC 1 cut(s) 953
PfeI GAWTC 2 cut(s) 419, 799
PkrI GCNGC 9 cut(s) 220, 223, 320, 542, 625, 628, 631, 634, 688
PpuMI RGGWCCY 2 cut(s) 887, 1044
PshBI ATTAAT 1 cut(s) 668
PsiI TTATAA 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 517
Psp5II RGGWCCY 2 cut(s) 887, 1044
PspFI CCCAGC 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 2 cut(s) 887, 1044
PspPPI RGGWCCY 2 cut(s) 887, 1044
PstI CTGCAG 1 cut(s) 223
PsuI RGATCY 3 cut(s) 545, 778, 842
PvuII CAGCTG 2 cut(s) 299, 479
SacI GAGCTC 1 cut(s) 517
SapI GCTCTTC 2 cut(s) 297, 600
SaqAI TTAA 3 cut(s) 335, 585, 668
SatI GCNGC 9 cut(s) 219, 222, 319, 541, 624, 627, 630, 633, 687
Sau3AI GATC 7 cut(s) 56, 372, 473, 545, 554, 778, 842
Sau96I GGNCC 2 cut(s) 887, 1044
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 3 cut(s) 124, 517, 595
SfaNI GCATC 1 cut(s) 761
SfcI CTRYAG 2 cut(s) 106, 219
SinI GGWCC 2 cut(s) 887, 1044
SphI GCATGC 1 cut(s) 818
Sse9I AATT 6 cut(s) 161, 170, 356, 442, 647, 669
SsiI CCGC 4 cut(s) 318, 434, 541, 825
SspI AATATT 1 cut(s) 379
SspMI CTAG 2 cut(s) 828, 980
SstI GAGCTC 1 cut(s) 517
StyD4I CCNGG 1 cut(s) 855
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 3 cut(s) 181, 526, 916
TaqI TCGA 6 cut(s) 39, 55, 139, 312, 446, 869
TasI AATT 6 cut(s) 161, 170, 356, 442, 647, 669
TauI GCSGC 2 cut(s) 321, 543
TfiI GAWTC 2 cut(s) 419, 799
Tru1I TTAA 3 cut(s) 335, 585, 668
Tru9I TTAA 3 cut(s) 335, 585, 668
TscAI CASTG 3 cut(s) 190, 919, 933
TseI GCWGC 7 cut(s) 218, 221, 623, 626, 629, 632, 686
TspDTI ATGAA 6 cut(s) 183, 411, 654, 663, 956, 998
TspRI CASTG 3 cut(s) 190, 919, 933
VneI GTGCAC 1 cut(s) 120
VpaK11BI GGWCC 2 cut(s) 887, 1044
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 2 cut(s) 161, 170
XceI RCATGY 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 2 cut(s) 828, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.