FvH4_2g38540

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011843
Physical Location & Seq
Forward (+)
27857153 .. 27859135
1983 bp
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UTR
Exon/CDS
Intron
FvH4_2g38540.t1

Sequence Viewer

Length: 639 bp
ATGATGAAGAGGGCGGAGATCGATACGAGGCAACCGTTCCGCTCAGTGAAGGAAGCTGTCGCATTGTTCGGCGAACGAGTTCTTGCCGGGGAGGTCTATGCCACCAAGCTTAAACAGTTGCAGGATGAAGCAAGTGAAAATGGGCATGGCACATCCAAAATTGGAACTGTAACAGCTGAGCTAGAAGAGACAAAGCAGAACCTCCAGAAAGCTAAACAAGAAAGTGAGCTAATGGAAACTTGCCTTTCTTCTCTGAAACAAGAGCTTGATCGGACAAAGCGAGAGCTGCAGCAACTCAAGGAAAGGGAATATGACAAACACTTTATAGAATCCGATGAGACTGAAGACGTTAAGATTGTTGAGGACGATCATTCAACCAAAATCGAAAACAAAAACAGCACACAAAGAAACAACGTAGCTGATATGAATGATGTTGGACTTGAATTCCAAAAGAAGAGATATGTGACATTTGCGCATCCACCTTCTGTGGCAAAAGTAATGATTCCACAAGGTGGTGCTGGTGTTGAGAAACTGGAAAGGCAGTCATCGCTTAAGAAGAAGAAAAAGAAGCCACTGATTCCTCTAATCGCAGGCATCTTTTCGAAGAAGAAAGGAAGCGATCCGGTAGCTTATGCTTGA

Protein Analysis

213

Amino Acids

23.98

Weight (kDa)

8.74

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 44 - 123 1e-06 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 474
AccB7I CCANNNNNTGG 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 42
AciI CCGC 2 cut(s) 14, 40
AclWI GGATC 1 cut(s) 614
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 363
AdeI CACNNNGTG 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 512
AflII CTTAAG 1 cut(s) 551
AgsI TTSAA 2 cut(s) 375, 443
Alw26I GTCTC 2 cut(s) 182, 332
AlwI GGATC 1 cut(s) 614
ApeKI GCWGC 2 cut(s) 286, 289
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 475
AsuC2I CCSGG 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 273, 301
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 182, 332
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 287
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 2 cut(s) 287, 290
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 2 cut(s) 288, 291
Bme1390I CCNGG 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 484, 603
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 88
Bsa29I ATCGAT 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 622
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 537
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 130, 152, 475
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 2 cut(s) 57, 168
BseNI ACTGG 1 cut(s) 537
BseXI GCAGC 2 cut(s) 273, 301
BshVI ATCGAT 1 cut(s) 21
BsiSI CCGG 2 cut(s) 87, 623
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 2 cut(s) 182, 332
Bsp119I TTCGAA 1 cut(s) 602
Bsp143I GATC 4 cut(s) 18, 268, 367, 619
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 2 cut(s) 14, 40
BspCNI CTCAG 2 cut(s) 56, 169
BspDI ATCGAT 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 291
BspPI GGATC 1 cut(s) 614
BspT104I TTCGAA 1 cut(s) 602
BspTI CTTAAG 1 cut(s) 551
BsrBI CCGCTC 1 cut(s) 42
BsrI ACTGG 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 4 cut(s) 18, 268, 367, 619
Bst4CI ACNGT 3 cut(s) 36, 117, 169
Bst6I CTCTTC 3 cut(s) 2, 180, 449
BstAFI CTTAAG 1 cut(s) 551
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 2 cut(s) 43, 177
BstF5I GGATG 3 cut(s) 130, 152, 475
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 4 cut(s) 21, 271, 370, 622
BstMAI GTCTC 2 cut(s) 182, 332
BstMBI GATC 4 cut(s) 18, 268, 367, 619
BstMWI GCNNNNNNNGC 2 cut(s) 286, 547
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 273, 301
BstV2I GAAGAC 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 21
BsuTUI ATCGAT 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 3 cut(s) 130, 152, 475
BtsIMutI CAGTG 2 cut(s) 51, 572
Cac8I GCNNGC 1 cut(s) 592
CfoI GCGC 1 cut(s) 475
ClaI ATCGAT 1 cut(s) 21
CviAII CATG 1 cut(s) 146
DdeI CTNAG 2 cut(s) 43, 177
DpnI GATC 4 cut(s) 20, 270, 369, 621
DpnII GATC 4 cut(s) 18, 268, 367, 619
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 3 cut(s) 2, 180, 449
EarI CTCTTC 3 cut(s) 2, 180, 449
EciI GGCGGA 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 443
FaeI CATG 1 cut(s) 149
FaiI YATR 7 cut(s) 99, 147, 312, 326, 425, 462, 633
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 287, 290
FokI GGATG 3 cut(s) 137, 139, 462
Fsp4HI GCNGC 2 cut(s) 287, 290
FspBI CTAG 1 cut(s) 182
FspI TGCGCA 1 cut(s) 474
GlaI GCGC 1 cut(s) 474
GluI GCNGC 2 cut(s) 287, 290
GsuI CTGGAG 1 cut(s) 188
HapII CCGG 2 cut(s) 87, 623
HhaI GCGC 1 cut(s) 475
Hin1II CATG 1 cut(s) 149
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 3 cut(s) 329, 502, 577
HpaII CCGG 2 cut(s) 87, 623
Hpy188I TCNGA 3 cut(s) 255, 273, 334
Hpy188III TCNNGA 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 43, 492
HpyCH4III ACNGT 3 cut(s) 36, 117, 169
HpyCH4IV ACGT 2 cut(s) 348, 414
HpyCH4V TGCA 2 cut(s) 121, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 547
HpyF3I CTNAG 2 cut(s) 43, 177
HpySE526I ACGT 2 cut(s) 348, 414
Hsp92II CATG 1 cut(s) 149
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 4 cut(s) 18, 268, 367, 619
LpnPI CCDG 6 cut(s) 100, 107, 218, 504, 518, 576
Lsp1109I GCAGC 2 cut(s) 273, 301
LweI GCATC 2 cut(s) 484, 603
MaeI CTAG 1 cut(s) 182
MaeII ACGT 2 cut(s) 348, 414
MaeIII GTNAC 2 cut(s) 169, 463
MalI GATC 4 cut(s) 20, 270, 369, 621
MbiI CCGCTC 1 cut(s) 42
MboI GATC 4 cut(s) 18, 268, 367, 619
MboII GAAGA 9 cut(s) 19, 197, 240, 356, 466, 568, 571, 616, 619
MluCI AATT 2 cut(s) 159, 443
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 6 cut(s) 3, 21, 85, 212, 355, 591
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 3 cut(s) 111, 351, 552
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 2 cut(s) 87, 623
MspR9I CCNGG 1 cut(s) 88
MwoI GCNNNNNNNGC 2 cut(s) 286, 547
NciI CCSGG 1 cut(s) 88
NdeII GATC 4 cut(s) 18, 268, 367, 619
NlaIII CATG 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 463
NsbI TGCGCA 1 cut(s) 474
NspV TTCGAA 1 cut(s) 602
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 277
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 329, 502, 577
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 2 cut(s) 288, 291
PstI CTGCAG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 176
SaqAI TTAA 3 cut(s) 111, 351, 552
SatI GCNGC 2 cut(s) 287, 290
Sau3AI GATC 4 cut(s) 18, 268, 367, 619
ScrFI CCNGG 1 cut(s) 88
SfaNI GCATC 2 cut(s) 484, 603
SfcI CTRYAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 296, 551
SmoI CTYRAG 2 cut(s) 296, 551
Sse9I AATT 2 cut(s) 159, 443
SsiI CCGC 2 cut(s) 14, 40
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 86
TaaI ACNGT 3 cut(s) 36, 117, 169
TaiI ACGT 2 cut(s) 351, 417
TaqI TCGA 3 cut(s) 21, 384, 602
TasI AATT 2 cut(s) 159, 443
TfiI GAWTC 3 cut(s) 329, 502, 577
Tru1I TTAA 3 cut(s) 111, 351, 552
Tru9I TTAA 3 cut(s) 111, 351, 552
TscAI CASTG 2 cut(s) 51, 579
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 2 cut(s) 286, 289
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 20, 141, 440
TspRI CASTG 2 cut(s) 51, 579
Van91I CCANNNNNTGG 1 cut(s) 512
Vha464I CTTAAG 1 cut(s) 551
XapI RAATTY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.