MD08G1013500.v1.1

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011843
Physical Location & Seq
Forward (+)
1028125 .. 1030217
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1013500.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGAGAAAGAAGTAGGAGGAGGAAGAGGAGGAGGAATGCAAACGACGAGGACAAGGAGGGCGGAGATTGATACGAGGCGACCCTTCCGCTCTGTCAAAGAGGCCGTTGCGTTGTTCGGAGAAAAAGTTCTTGCCGGAGAGCTCTATGCCTCCCAGCTCAAACAGATGCAGAATGAAGCAAATAGAAATGGGCATAATCCTGCATTATCCAGAATTGGAACTGTGACAGCAGAACTAGAGGACACAAAACAAAGCCTCCAGAAAGCCAGAGAAGAAAGTGAGCTCATGGAAAACTGCCTCTCTTCTCTAAAACAGGAGCTCGATCTCACAAAGCGAGAGCTCCAGCAACTGAAGGAACGAGAGTACGAAAAACACTTCATAGAAACCGAGATTGAAGACGTCAGGACATTCGACGAAGACTCGACCAAGTTTGAAACCAAACCACAAGCATCTAGTGAGGAAGATGAAAGTTTTGAGTTCCAAAAGAAAAGGTATGTGACATTTGCAAATCCACCTTCCCTTGCGAAAGTGATGATTGCGCCGGGAGGGGTTGAGGAAACACTGGAAAGACACCCTTCTCTCAAGAAAAAGAAGAAGCCCTTGATTCCTCTGATTTCAGGGATCTTTTCTAAGAAGAAAGGAAGCTCGCCAGTCGCTTACGCTTGA

Protein Analysis

222

Amino Acids

25.01

Weight (kDa)

7.76

Isoelectric Point (pI)

61.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 50 - 122 4.7e-06 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 402
AccBSI CCGCTC 1 cut(s) 90
AciI CCGC 2 cut(s) 62, 88
AclWI GGATC 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 2 cut(s) 395, 434
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 6 cut(s) 142, 157, 283, 319, 340, 645
AluI AGCT 6 cut(s) 142, 157, 283, 319, 340, 645
Alw21I GWGCWC 4 cut(s) 144, 285, 321, 342
AlwI GGATC 1 cut(s) 629
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 541
AsuC2I CCSGG 1 cut(s) 543
BanII GRGCYC 4 cut(s) 144, 285, 321, 342
BbsI GAAGAC 2 cut(s) 402, 423
Bbv12I GWGCWC 4 cut(s) 144, 285, 321, 342
BceAI ACGGC 1 cut(s) 89
BcnI CCSGG 1 cut(s) 543
BfaI CTAG 2 cut(s) 236, 453
Bme1390I CCNGG 1 cut(s) 543
BmrFI CCNGG 1 cut(s) 543
BmsI GCATC 2 cut(s) 156, 458
BpiI GAAGAC 2 cut(s) 402, 423
BpmI CTGGAG 2 cut(s) 242, 326
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 399
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 240, 270
Bse1I ACTGG 2 cut(s) 567, 650
BseNI ACTGG 2 cut(s) 567, 650
BseRI GAGGAG 3 cut(s) 33, 42, 45
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 1 cut(s) 104
BsiHKAI GWGCWC 4 cut(s) 144, 285, 321, 342
BsiSI CCGG 2 cut(s) 135, 542
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 4 cut(s) 144, 285, 321, 342
Bsp143I GATC 2 cut(s) 322, 621
BspACI CCGC 2 cut(s) 62, 88
BspANI GGCC 1 cut(s) 104
BspPI GGATC 1 cut(s) 629
BsrBI CCGCTC 1 cut(s) 90
BsrI ACTGG 2 cut(s) 567, 650
BssMI GATC 2 cut(s) 322, 621
BssNI GRCGYC 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 19, 307
BstACI GRCGYC 1 cut(s) 399
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 1 cut(s) 630
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 2 cut(s) 325, 624
BstMBI GATC 2 cut(s) 322, 621
BstSCI CCNGG 1 cut(s) 541
BstV2I GAAGAC 2 cut(s) 402, 423
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuRI GGCC 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 560
Cac8I GCNNGC 1 cut(s) 647
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 364
CviAII CATG 1 cut(s) 286
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 630
DpnI GATC 2 cut(s) 324, 623
DpnII GATC 2 cut(s) 322, 621
Eam1104I CTCTTC 2 cut(s) 19, 307
EarI CTCTTC 2 cut(s) 19, 307
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 4 cut(s) 142, 283, 319, 340
Eco24I GRGCYC 4 cut(s) 144, 285, 321, 342
Eco53kI GAGCTC 4 cut(s) 142, 283, 319, 340
Eco57I CTGAAG 1 cut(s) 371
EcoICRI GAGCTC 4 cut(s) 142, 283, 319, 340
EcoT38I GRGCYC 4 cut(s) 144, 285, 321, 342
FaeI CATG 1 cut(s) 289
FaiI YATR 5 cut(s) 147, 195, 287, 380, 495
FalI AAGNNNNNCTT 4 cut(s) 559, 591, 584, 616
FatI CATG 1 cut(s) 285
FriOI GRGCYC 4 cut(s) 144, 285, 321, 342
FspBI CTAG 2 cut(s) 236, 453
GlaI GCGC 1 cut(s) 540
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 2 cut(s) 242, 326
HaeIII GGCC 1 cut(s) 104
HapII CCGG 2 cut(s) 135, 542
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HinfI GANTC 2 cut(s) 419, 604
HpaII CCGG 2 cut(s) 135, 542
Hpy188I TCNGA 2 cut(s) 119, 612
Hpy188III TCNNGA 4 cut(s) 210, 259, 403, 583
Hpy99I CGWCG 2 cut(s) 49, 416
HpyAV CCTTC 4 cut(s) 94, 346, 525, 585
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 40, 169, 203, 506
HpyF3I CTNAG 1 cut(s) 630
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 1 cut(s) 539
Kzo9I GATC 2 cut(s) 322, 621
LmnI GCTCC 2 cut(s) 316, 345
LweI GCATC 2 cut(s) 156, 458
MaeI CTAG 2 cut(s) 236, 453
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 223, 496
MalI GATC 2 cut(s) 324, 623
MbiI CCGCTC 1 cut(s) 90
MboI GATC 2 cut(s) 322, 621
MboII GAAGA 8 cut(s) 36, 284, 294, 407, 428, 473, 604, 646
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 4 cut(s) 144, 285, 321, 342
MluCI AATT 1 cut(s) 213
MlyI GAGTC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 126
MspI CCGG 2 cut(s) 135, 542
MspR9I CCNGG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 543
NdeII GATC 2 cut(s) 322, 621
NlaIII CATG 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 223, 496
PctI GAATGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 604
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp124BI GAGCTC 4 cut(s) 144, 285, 321, 342
PspFI CCCAGC 1 cut(s) 153
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 621
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SacI GAGCTC 4 cut(s) 144, 285, 321, 342
Sau3AI GATC 2 cut(s) 322, 621
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 4 cut(s) 144, 285, 321, 342
SetI ASST 9 cut(s) 144, 159, 285, 321, 342, 402, 494, 517, 647
SfaNI GCATC 2 cut(s) 156, 458
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 1 cut(s) 213
SsiI CCGC 2 cut(s) 62, 88
SspMI CTAG 2 cut(s) 236, 453
SstI GAGCTC 4 cut(s) 144, 285, 321, 342
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 402
TaqI TCGA 3 cut(s) 321, 411, 422
TasI AATT 1 cut(s) 213
TfiI GAWTC 1 cut(s) 604
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 2 cut(s) 223, 496
Tsp45I GTSAC 2 cut(s) 223, 496
TspDTI ATGAA 3 cut(s) 189, 367, 480
TspRI CASTG 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 2 cut(s) 236, 453
ZraI GACGTC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.