pycom08g01190

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011843
Physical Location & Seq
Forward (+)
894912 .. 897054
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01190.1

Sequence Viewer

Length: 795 bp
ATGATAGACCACCCCATCTGTCTCTCCCTCTCTCTCTCTCTCTCAAGTAGTACTAGTCCAAATAGAACACAAATTCTTTCCCTTCATTCTTTCAACTTTAGGAGTGCAACATTTTACAAAGTGAACATGGAGAAAGAAGTAGGAGGAGGAGGAGGAGGAGGAGGAATGCAAACGACGAGGACGAGGAGGGCGGAGATTGATACGAGGCGACCCTTCCGCTCTGTCAAAGAGGCCGTTGCGTTGTTCGGAGAAAAAGTTCTTGCCGGAGAGCTCTATGCCTCCCAGCTCAAACAGATGCAGAATGAAGCAAATGGAAATGGGCATCACCCTGCATTATCCAGAATTGGAACTGTGACAGCAGAACTAGAGGACACAAAACAAAGCCTCCAGAAAGCCAGAGAAGAAAGTGAGCTCATGGAAAACTGCCTCTCTTCTCTAAAACAGGAGCTCGATCGCACAAAGCAAGAGCTCCAGCAACTGAAGGAACGAGAGTATGAAAAACACTTCATAGAAACCGAGATTGAAGACGTCAGGATATTCGACGAAGACTCGACCAAGTTTGAAACCAAACCACAAGCATCTAGTGAGGAAGATGAAAGTTTTGAGTTCCAAAAGAAAAGGTATGTGACATTTGCAAATCCACCTTCCCTTGCGAAAGTGATGATTGCGCCAGGAGGTGTTGAGGAAACACTGCAAAGACACCCTTCTCTCAAGAAAAAAAAGAAGCCCTTGATTCCTCTGATTGCAGGGATCTTTTCTAAGAAGAAAGGAAGCTCCCCAGTTGCTTATGCTTGA

Protein Analysis

265

Amino Acids

29.6

Weight (kDa)

8.38

Isoelectric Point (pI)

60.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 90 - 165 4.9e-07 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 531
AccBSI CCGCTC 1 cut(s) 219
AciI CCGC 2 cut(s) 191, 217
AclWI GGATC 1 cut(s) 758
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 1 cut(s) 52
AgsI TTSAA 3 cut(s) 94, 524, 563
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 670
AloI GAACNNNNNNTCC 2 cut(s) 240, 272
AluBI AGCT 6 cut(s) 271, 286, 412, 448, 469, 774
AluI AGCT 6 cut(s) 271, 286, 412, 448, 469, 774
Alw21I GWGCWC 4 cut(s) 273, 414, 450, 471
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 758
AlwNI CAGNNNCTG 1 cut(s) 478
AoxI GGCC 1 cut(s) 231
ApoI RAATTY 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 670
AsuHPI GGTGA 1 cut(s) 317
BanII GRGCYC 4 cut(s) 273, 414, 450, 471
BbsI GAAGAC 2 cut(s) 531, 552
Bbv12I GWGCWC 4 cut(s) 273, 414, 450, 471
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 672
BcoDI GTCTC 1 cut(s) 26
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 3 cut(s) 54, 365, 582
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 672
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 3 cut(s) 285, 331, 587
BmuI ACTGGG 1 cut(s) 773
BpiI GAAGAC 2 cut(s) 531, 552
BpmI CTGGAG 2 cut(s) 371, 455
BpuEI CTTGAG 2 cut(s) 28, 695
BsaHI GRCGYC 1 cut(s) 528
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 369, 399
Bse1I ACTGG 1 cut(s) 779
BseBI CCWGG 1 cut(s) 672
BseNI ACTGG 1 cut(s) 779
BseRI GAGGAG 7 cut(s) 159, 162, 165, 168, 171, 174, 199
BseYI CCCAGC 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 4 cut(s) 273, 414, 450, 471
BsiSI CCGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 4 cut(s) 273, 414, 450, 471
Bsp143I GATC 2 cut(s) 451, 750
BspACI CCGC 2 cut(s) 191, 217
BspANI GGCC 1 cut(s) 233
BspPI GGATC 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 219
BsrI ACTGG 1 cut(s) 779
BssMI GATC 2 cut(s) 451, 750
BssNI GRCGYC 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 528
BstDEI CTNAG 1 cut(s) 759
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 2 cut(s) 454, 753
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 2 cut(s) 451, 750
BstMCI CGRYCG 1 cut(s) 454
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 670
BstV2I GAAGAC 2 cut(s) 531, 552
BstX2I RGATCY 1 cut(s) 750
BstYI RGATCY 1 cut(s) 750
BsuRI GGCC 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 1 cut(s) 689
CaiI CAGNNNCTG 1 cut(s) 478
CfoI GCGC 1 cut(s) 670
Csp6I GTAC 1 cut(s) 51
CviAII CATG 2 cut(s) 127, 415
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 759
DpnI GATC 2 cut(s) 453, 752
DpnII GATC 2 cut(s) 451, 750
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 4 cut(s) 271, 412, 448, 469
Eco24I GRGCYC 4 cut(s) 273, 414, 450, 471
Eco53kI GAGCTC 4 cut(s) 271, 412, 448, 469
Eco57I CTGAAG 1 cut(s) 500
EcoICRI GAGCTC 4 cut(s) 271, 412, 448, 469
EcoRII CCWGG 1 cut(s) 670
EcoT38I GRGCYC 4 cut(s) 273, 414, 450, 471
FaeI CATG 2 cut(s) 130, 418
FaiI YATR 7 cut(s) 128, 276, 416, 495, 509, 624, 789
FalI AAGNNNNNCTT 4 cut(s) 688, 720, 713, 745
FatI CATG 2 cut(s) 126, 414
FriOI GRGCYC 4 cut(s) 273, 414, 450, 471
FspBI CTAG 3 cut(s) 54, 365, 582
GlaI GCGC 1 cut(s) 669
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 2 cut(s) 371, 455
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 670
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 2 cut(s) 130, 418
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HinfI GANTC 2 cut(s) 548, 733
HpaII CCGG 1 cut(s) 264
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 248, 741
Hpy188III TCNNGA 4 cut(s) 339, 388, 532, 712
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 2 cut(s) 178, 545
HpyAV CCTTC 5 cut(s) 92, 223, 475, 654, 714
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 7 cut(s) 107, 169, 298, 332, 635, 694, 746
HpyF3I CTNAG 1 cut(s) 759
HpySE526I ACGT 1 cut(s) 528
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 2 cut(s) 130, 418
HspAI GCGC 1 cut(s) 668
Kzo9I GATC 2 cut(s) 451, 750
LmnI GCTCC 3 cut(s) 445, 474, 779
LweI GCATC 3 cut(s) 285, 331, 587
MaeI CTAG 3 cut(s) 54, 365, 582
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 2 cut(s) 352, 625
MalI GATC 2 cut(s) 453, 752
MbiI CCGCTC 1 cut(s) 219
MboI GATC 2 cut(s) 451, 750
MboII GAAGA 6 cut(s) 413, 423, 536, 557, 602, 775
MflI RGATCY 1 cut(s) 750
MhlI GDGCHC 4 cut(s) 273, 414, 450, 471
MluCI AATT 2 cut(s) 72, 342
MlyI GAGTC 1 cut(s) 542
MroXI GAANNNNTTC 1 cut(s) 255
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 672
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 1 cut(s) 672
NdeII GATC 2 cut(s) 451, 750
NlaIII CATG 2 cut(s) 130, 418
NmuCI GTSAC 2 cut(s) 352, 625
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PctI GAATGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 733
Ple19I CGATCG 1 cut(s) 454
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
Psp124BI GAGCTC 4 cut(s) 273, 414, 450, 471
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 750
PvuI CGATCG 1 cut(s) 454
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
SacI GAGCTC 4 cut(s) 273, 414, 450, 471
Sau3AI GATC 2 cut(s) 451, 750
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 4 cut(s) 273, 414, 450, 471
SfaNI GCATC 3 cut(s) 285, 331, 587
SmlI CTYRAG 2 cut(s) 43, 710
SmoI CTYRAG 2 cut(s) 43, 710
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 2 cut(s) 72, 342
SsiI CCGC 2 cut(s) 191, 217
SspMI CTAG 3 cut(s) 54, 365, 582
SstI GAGCTC 4 cut(s) 273, 414, 450, 471
StyD4I CCNGG 1 cut(s) 670
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 1 cut(s) 531
TaqI TCGA 3 cut(s) 450, 540, 551
TasI AATT 2 cut(s) 72, 342
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 733
TscAI CASTG 1 cut(s) 696
TseFI GTSAC 2 cut(s) 352, 625
Tsp45I GTSAC 2 cut(s) 352, 625
TspDTI ATGAA 5 cut(s) 74, 318, 496, 510, 609
TspRI CASTG 1 cut(s) 696
XapI RAATTY 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 3 cut(s) 54, 365, 582
ZraI GACGTC 1 cut(s) 529
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.