Rh6CG442700

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011843
Physical Location & Seq
Reverse (-)
62170407 .. 62172591
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG442700.1

Sequence Viewer

Length: 642 bp
ATGATGAAGAGGGCGGAGATCGATACAAGGCAACCGTTCCGGTCAGTGAAGGAAGCTGTTGCACTGTTCGGTGAAAGAGTTCTTGCCGGAGAGGTTTATGCCACCAAGCTCAAACAGTTGCAGGATGGAGCAAGTGAAAATGGGCATGGCACATCGAAAGTTGGAACTATAACAGCTGAGCTAGAGGAGACAAGGCAGAACCTCCAAAAAGCCAAACAGGAAAGTGAGCTAATGGAAACTTGCCTTTCTTCTCTGAAACAAGAGCTTGATCGGACGAAGCGAGAGCTGCAGCAACTCAAGGAGAGGGAATATGACAAACACTTTATAGAATCCGATGAGATTGAAGACGTTAAGATTGTTGAAGATGATCGTTCAGCCAAAATCGAAAGCAAAAACAGCACACGAAGAAACAATGTAGCTGACATGGATGATGAAGGACTTGAATTCCAAAAGAAGAGATATGTGACATTTTCACACCCACCTTCTGTGGCTAAAGTAATGATTCCACAAGGTGGTGCTGATGTTGAGAAATTGGAAAGACATTCATCATCAATTAAGAAGAAGAAAAAGAAGCCATTGATTCCTCTAATTGCAGGTATCTTTTCGAAGAAGAAAGGAAGCCATCCAGTAGCCTATGCTTGA

Protein Analysis

213

Amino Acids

24.16

Weight (kDa)

8.95

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 46 - 104 9.6e-07 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 584
AccB7I CCANNNNNTGG 1 cut(s) 512
AciI CCGC 1 cut(s) 14
AcsI RAATTY 1 cut(s) 443
AdeI CACNNNGTG 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 3 cut(s) 344, 362, 443
AluBI AGCT 8 cut(s) 56, 109, 176, 181, 229, 265, 286, 419
AluI AGCT 8 cut(s) 56, 109, 176, 181, 229, 265, 286, 419
Alw26I GTCTC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 286, 289
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 83
AsuII TTCGAA 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 273, 301
BccI CCATC 2 cut(s) 119, 630
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 584
BisI GCNGC 2 cut(s) 287, 290
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 2 cut(s) 288, 291
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse1I ACTGG 1 cut(s) 626
BseCI ATCGAT 1 cut(s) 21
BseGI GGATG 3 cut(s) 130, 433, 622
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 273, 301
BshVI ATCGAT 1 cut(s) 21
BsiSI CCGG 2 cut(s) 40, 87
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 605
Bsp143I GATC 3 cut(s) 18, 268, 367
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 291
BspMI ACCTGC 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 605
BsrI ACTGG 1 cut(s) 626
BssMI GATC 3 cut(s) 18, 268, 367
Bst4CI ACNGT 3 cut(s) 36, 66, 117
Bst6I CTCTTC 2 cut(s) 2, 449
BstBI TTCGAA 1 cut(s) 605
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 130, 433, 622
BstKTI GATC 3 cut(s) 21, 271, 370
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 3 cut(s) 18, 268, 367
BstMWI GCNNNNNNNGC 2 cut(s) 286, 396
BstSFI CTRYAG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 273, 301
BstV2I GAAGAC 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 21
BsuTUI ATCGAT 1 cut(s) 21
BtsCI GGATG 3 cut(s) 130, 433, 622
BtsIMutI CAGTG 2 cut(s) 51, 62
BveI ACCTGC 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 21
CviAII CATG 2 cut(s) 146, 424
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 20, 270, 369
DpnII GATC 3 cut(s) 18, 268, 367
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 2 cut(s) 2, 449
EarI CTCTTC 2 cut(s) 2, 449
EciI GGCGGA 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 443
FaeI CATG 2 cut(s) 149, 427
FaiI YATR 8 cut(s) 99, 147, 170, 312, 326, 425, 462, 636
FatI CATG 2 cut(s) 145, 423
Fnu4HI GCNGC 2 cut(s) 287, 290
FokI GGATG 3 cut(s) 137, 440, 609
Fsp4HI GCNGC 2 cut(s) 287, 290
FspBI CTAG 1 cut(s) 182
GluI GCNGC 2 cut(s) 287, 290
HapII CCGG 2 cut(s) 40, 87
Hin1II CATG 2 cut(s) 149, 427
HinfI GANTC 3 cut(s) 329, 502, 580
HpaII CCGG 2 cut(s) 40, 87
HphI GGTGA 1 cut(s) 83
Hpy188I TCNGA 3 cut(s) 255, 273, 334
HpyAV CCTTC 3 cut(s) 43, 428, 492
HpyCH4III ACNGT 3 cut(s) 36, 66, 117
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 62, 121, 289, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 396
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 2 cut(s) 149, 427
Kzo9I GATC 3 cut(s) 18, 268, 367
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 5 cut(s) 53, 100, 107, 203, 579
Lsp1109I GCAGC 2 cut(s) 273, 301
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 463
MalI GATC 3 cut(s) 20, 270, 369
MboI GATC 3 cut(s) 18, 268, 367
MluCI AATT 4 cut(s) 443, 530, 552, 588
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 6 cut(s) 3, 85, 178, 212, 297, 594
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 2 cut(s) 351, 555
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 2 cut(s) 40, 87
MwoI GCNNNNNNNGC 2 cut(s) 286, 396
NdeII GATC 3 cut(s) 18, 268, 367
NlaIII CATG 2 cut(s) 149, 427
NmuCI GTSAC 1 cut(s) 463
NspV TTCGAA 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 329, 502, 580
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 2 cut(s) 288, 291
PstI CTGCAG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 176
SaqAI TTAA 2 cut(s) 351, 555
SatI GCNGC 2 cut(s) 287, 290
Sau3AI GATC 3 cut(s) 18, 268, 367
SfcI CTRYAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 605
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 4 cut(s) 443, 530, 552, 588
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 182
TaaI ACNGT 3 cut(s) 36, 66, 117
TaiI ACGT 1 cut(s) 351
TaqI TCGA 4 cut(s) 21, 155, 384, 605
TasI AATT 4 cut(s) 443, 530, 552, 588
TfiI GAWTC 3 cut(s) 329, 502, 580
Tru1I TTAA 2 cut(s) 351, 555
Tru9I TTAA 2 cut(s) 351, 555
TscAI CASTG 2 cut(s) 51, 69
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 2 cut(s) 286, 289
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 20, 447, 534
TspRI CASTG 2 cut(s) 51, 69
Van91I CCANNNNNTGG 1 cut(s) 512
XapI RAATTY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.