MD15G1013000.v1.1

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011843
Physical Location & Seq
Forward (+)
715671 .. 717719
2049 bp
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UTR
Exon/CDS
Intron
MD15G1013000.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGAGAAAGAAGTAGGAGGAGGAGGAGGAGGAGGAATGCAAGTGACGAGGACAAGGAGGGCGGAGATCGATACAAGGCAACCGTTCCGCTCCGTCAAAGAGGCAGTTGCGTTGTTCGGAGAAAAAGTTCTTGCCGGAGAGCTCTATGCCTCCCAGCTCAAACAGATGCAGAACGAAGCAAATGAAAATGGGCATGGCCCCGCATTATCCAGAATTGGAACTGTGAGAGCAGAACTAGAGGACACAAAACAAAGCCTCCAAAAAGCCAGACAAGAAAGTGAGCTCATGGAAAACTGCCTCTCTTCTCTAAAACAGGAGCTCGAACGCACAAAGCGAGAGCTCCAGCAACTGAAGGAACGGGAGTACGAAAAACACTTCATCGAAACGGAGATTGAAGACGTTAGGATTGTCGACGAAGACTCGACCAAGTTTACAACCAAAACACAAGCATCTGGTGAGGAAGATGAAAGTTTTGAGTTCCAAAAGAAAAGATATGTGACATTTGGAAATCCACCTTCTCGGTCTCAAGTGATGATTGCACAAGGTGGTGTTGATCAAACATTGGAAAGACACCATTCTCTCACGAAAAAGAAAAAGAAGCCCATGATTCCTCTGATCGCAGGGATCTTTTCTAAGAAGAAAGGAAGCTCACAGGTTGCTTATGCTTGA

Protein Analysis

223

Amino Acids

25.05

Weight (kDa)

8.5

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 48 - 123 3.6e-07 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75720 AT1G75720 AT1G75720
fragaria_vesca FvH4_2g38540
malus_domestica MD08G1013500.v1.1 MD15G1013000.v1.1
prunus_persica Prupe.1G366100_v2.0.a1
pyrus_communis pycom08g01190 pycom15g01130
rosa_chinensis RchiOBHm_Chr6g0302771
rosa_laevigata RLG00000011139
rosa_roxburghii Rroxscaffold_7G00165340
rosa_rugosa Rorug06G0316700 Rorug06G0316800
rosa_samantha Rh6AG430200 Rh6BG472400 Rh6CG442700 Rh6DG429400
rosa_wichuraiana Rw6G037260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 411
AciI CCGC 3 cut(s) 62, 88, 201
AclWI GGATC 1 cut(s) 632
AcuI CTGAAG 1 cut(s) 371
AdeI CACNNNGTG 1 cut(s) 545
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 1 cut(s) 395
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 6 cut(s) 142, 157, 283, 319, 340, 648
AluI AGCT 6 cut(s) 142, 157, 283, 319, 340, 648
Alw21I GWGCWC 4 cut(s) 144, 285, 321, 342
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 467
BanII GRGCYC 4 cut(s) 144, 285, 321, 342
BbsI GAAGAC 2 cut(s) 402, 423
Bbv12I GWGCWC 4 cut(s) 144, 285, 321, 342
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 2 cut(s) 156, 458
BpiI GAAGAC 2 cut(s) 402, 423
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 510
Bsa29I ATCGAT 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 528
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 240, 270
BseCI ATCGAT 1 cut(s) 69
BseRI GAGGAG 5 cut(s) 33, 36, 39, 42, 45
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 1 cut(s) 198
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 4 cut(s) 144, 285, 321, 342
BsiSI CCGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 528
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 528
Bsp1286I GDGCHC 4 cut(s) 144, 285, 321, 342
Bsp143I GATC 4 cut(s) 66, 553, 615, 624
BspACI CCGC 3 cut(s) 62, 88, 201
BspANI GGCC 1 cut(s) 198
BspDI ATCGAT 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 528
BsrBI CCGCTC 1 cut(s) 90
BssMI GATC 4 cut(s) 66, 553, 615, 624
Bst4CI ACNGT 2 cut(s) 84, 223
Bst6I CTCTTC 1 cut(s) 307
BstDEI CTNAG 1 cut(s) 633
BstKTI GATC 4 cut(s) 69, 556, 618, 627
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 4 cut(s) 66, 553, 615, 624
BstV2I GAAGAC 2 cut(s) 402, 423
BstX2I RGATCY 1 cut(s) 624
BstYI RGATCY 1 cut(s) 624
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 1 cut(s) 198
BsuTUI ATCGAT 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 197
ClaI ATCGAT 1 cut(s) 69
Csp6I GTAC 1 cut(s) 364
CviAII CATG 3 cut(s) 194, 286, 604
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 633
DpnI GATC 4 cut(s) 68, 555, 617, 626
DpnII GATC 4 cut(s) 66, 553, 615, 624
DraIII CACNNNGTG 1 cut(s) 545
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 4 cut(s) 142, 283, 319, 340
Eco24I GRGCYC 4 cut(s) 144, 285, 321, 342
Eco31I GGTCTC 1 cut(s) 528
Eco53kI GAGCTC 4 cut(s) 142, 283, 319, 340
Eco57I CTGAAG 1 cut(s) 371
EcoICRI GAGCTC 4 cut(s) 142, 283, 319, 340
EcoT38I GRGCYC 4 cut(s) 144, 285, 321, 342
FaeI CATG 3 cut(s) 197, 289, 607
FaiI YATR 6 cut(s) 147, 195, 287, 495, 605, 663
FatI CATG 3 cut(s) 193, 285, 603
FauI CCCGC 1 cut(s) 208
FbaI TGATCA 1 cut(s) 553
FblI GTMKAC 1 cut(s) 411
FriOI GRGCYC 4 cut(s) 144, 285, 321, 342
FspBI CTAG 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 1 cut(s) 198
HapII CCGG 1 cut(s) 135
Hin1II CATG 3 cut(s) 197, 289, 607
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 2 cut(s) 419, 607
HpaII CCGG 1 cut(s) 135
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 2 cut(s) 412, 432
Hpy188I TCNGA 2 cut(s) 119, 615
Hpy188III TCNNGA 2 cut(s) 210, 583
Hpy8I GTNNAC 2 cut(s) 412, 432
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 2 cut(s) 346, 525
HpyCH4III ACNGT 2 cut(s) 84, 223
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 40, 169, 539
HpyF3I CTNAG 1 cut(s) 633
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 3 cut(s) 197, 289, 607
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 66, 553, 615, 624
LmnI GCTCC 3 cut(s) 95, 316, 345
LpnPI CCDG 9 cut(s) 148, 167, 223, 280, 299, 356, 438, 606, 638
LweI GCATC 2 cut(s) 156, 458
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 43, 496
MalI GATC 4 cut(s) 68, 555, 617, 626
MbiI CCGCTC 1 cut(s) 90
MboI GATC 4 cut(s) 66, 553, 615, 624
MboII GAAGA 5 cut(s) 294, 407, 428, 473, 649
MflI RGATCY 1 cut(s) 624
MhlI GDGCHC 4 cut(s) 144, 285, 321, 342
MluCI AATT 1 cut(s) 213
MlyI GAGTC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 126
MspI CCGG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 42
NdeII GATC 4 cut(s) 66, 553, 615, 624
NlaIII CATG 3 cut(s) 197, 289, 607
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 43, 496
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PctI GAATGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 607
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp124BI GAGCTC 4 cut(s) 144, 285, 321, 342
PspFI CCCAGC 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 624
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SacI GAGCTC 4 cut(s) 144, 285, 321, 342
SalI GTCGAC 1 cut(s) 410
Sau3AI GATC 4 cut(s) 66, 553, 615, 624
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 1 cut(s) 413
SduI GDGCHC 4 cut(s) 144, 285, 321, 342
SfaNI GCATC 2 cut(s) 156, 458
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 1 cut(s) 213
SsiI CCGC 3 cut(s) 62, 88, 201
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 4 cut(s) 144, 285, 321, 342
TaaI ACNGT 2 cut(s) 84, 223
TaiI ACGT 1 cut(s) 402
TaqI TCGA 5 cut(s) 69, 321, 381, 411, 422
TaqII GACCGA 1 cut(s) 510
TasI AATT 1 cut(s) 213
TfiI GAWTC 1 cut(s) 607
TseFI GTSAC 2 cut(s) 43, 496
Tsp45I GTSAC 2 cut(s) 43, 496
TspDTI ATGAA 3 cut(s) 198, 367, 480
TspGWI ACGGA 2 cut(s) 82, 401
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.