FvH4_3g00011

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010498
Physical Location & Seq
Reverse (-)
18147 .. 20772
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00011.t1

Sequence Viewer

Length: 729 bp
ATGAATGCACTTACCCTATGTCACCATAACTATAATATTCATATCAATAGCCACCATCATTTCTCAGCGTCGGCGAAGCCCAATTGGCCCAAATTGTTGTCAGTTCCTCATCCCCGAAACCATGGCACTAGTACAAGGCCTTCGTCCCTGCTAATCGAGACGGTGGAAGGCCAAGTTGCCGCTGATTCCGAGCAAGCTCCTTCATCCGTTGCTCGTCGTCTCATTCTGCTTCGTCATGCCAAGAGTTCCTGGGAAGACCCCTCGCTAAAAGATCATGATCGACCCCTGAGTGAAAAAGGACAAGCGGATGCTGTCGAAATCTCTCACAAGCTCCAACAGTTGGGCTGGGTTCCTCAGCTTATTTTATCCAGCAATGCACTGCGAACCAGGGAGACTCTTAGAATAATGCAGCAACAAGTGAAAGGCTTCTTGGAAGCTGAGGTCCATTACTTTTCCAGCTTTTATTCCATTGCCGCCATGGATGGGCAGACTGCTGAGCACCTTCAACGTATTATCTGCAACTATTCAAGGGATGACATACTCACGGTCATGTGTATGGGACATAATAGGGGTTGGGAGGAGGCAGCATCAATGTTGACAGGTGCCTCCATAGAACTGAAGACTTGCAACGCTGCTTTGCTTGAAACTACTGGGAAATCTTGGGATGAGGCATTTGCTTTGGCAGGACATGGGGGGTGGAAACTGCAAGGCATTGTGAAACCAAGTTAG

Protein Analysis

243

Amino Acids

26.92

Weight (kDa)

6.95

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 74 - 144 6.2e-08 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52155 AT3G52155 AT3G52155 AT3G52155
fragaria_vesca FvH4_3g00011
malus_domestica MD10G1342400.v1.1
prunus_persica Prupe.4G000400_v2.0.a1
pyrus_communis pycom10g29170
rosa_chinensis RchiOBHm_Chr5g0000011
rosa_laevigata RLG00000030832
rosa_multiflora Rmu_sc0007644.1_g000011
rosa_roxburghii Rroxscaffold_1G00076050
rosa_rugosa Rorug04G0380000 Rorug04G0380100 Rorug04G0380200 Rorug04G0380300
rosa_samantha Rh5AG000200 Rh5BG001400 Rh5CG000200 Rh5DG000200
rosa_wichuraiana Rw5G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 602
AccB7I CCANNNNNTGG 1 cut(s) 340
AciI CCGC 3 cut(s) 180, 305, 474
AcuI CTGAAG 1 cut(s) 638
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 340, 483
AgsI TTSAA 3 cut(s) 506, 528, 644
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 2 cut(s) 248, 386
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 5 cut(s) 197, 331, 358, 437, 459
AluI AGCT 5 cut(s) 197, 331, 358, 437, 459
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 3 cut(s) 152, 224, 386
AoxI GGCC 3 cut(s) 86, 137, 169
ApeKI GCWGC 3 cut(s) 409, 584, 632
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 87, 442
AsuHPI GGTGA 1 cut(s) 14
AvaII GGWCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 602
BarI GAAGNNNNNNTAC 2 cut(s) 124, 156
BbsI GAAGAC 2 cut(s) 261, 626
Bbv12I GWGCWC 1 cut(s) 501
BbvCI CCTCAGC 2 cut(s) 354, 438
BbvI GCAGC 3 cut(s) 421, 596, 619
BccI CCATC 2 cut(s) 63, 476
BciT130I CCWGG 2 cut(s) 250, 388
BcoDI GTCTC 3 cut(s) 152, 224, 386
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 85
BisI GCNGC 5 cut(s) 180, 410, 474, 585, 633
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 5 cut(s) 181, 411, 475, 586, 634
Bme1390I CCNGG 2 cut(s) 250, 388
Bme18I GGWCC 1 cut(s) 442
BmgT120I GGNCC 2 cut(s) 87, 442
BmiI GGNNCC 2 cut(s) 351, 604
BmrFI CCNGG 2 cut(s) 250, 388
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 2 cut(s) 298, 596
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 261, 626
Bpu10I CCTNAGC 2 cut(s) 354, 438
Bpu1102I GCTNAGC 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 121, 249, 387, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 483
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 2 cut(s) 379, 468
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 2 cut(s) 250, 388
BseDI CCNNGG 4 cut(s) 121, 249, 387, 477
BseGI GGATG 6 cut(s) 109, 203, 313, 487, 538, 670
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 2 cut(s) 340, 483
BseMI GCAATG 2 cut(s) 379, 468
BseMII CTCAG 5 cut(s) 78, 278, 368, 429, 486
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 593
BseXI GCAGC 3 cut(s) 421, 596, 619
BseYI CCCAGC 1 cut(s) 345
BshFI GGCC 3 cut(s) 88, 139, 171
BshNI GGYRCC 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 501
BslFI GGGAC 2 cut(s) 130, 573
BslI CCNNNNNNNGG 2 cut(s) 340, 483
BsmAI GTCTC 3 cut(s) 152, 224, 386
BsmBI CGTCTC 2 cut(s) 152, 224
BsmFI GGGAC 2 cut(s) 130, 573
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 88, 139, 171
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 2 cut(s) 271, 277
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 2 cut(s) 121, 477
BspACI CCGC 3 cut(s) 180, 305, 474
BspANI GGCC 3 cut(s) 88, 139, 171
BspCNI CTCAG 5 cut(s) 77, 279, 367, 430, 487
BspHI TCATGA 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 351, 604
BspT107I GGYRCC 1 cut(s) 602
BsrDI GCAATG 2 cut(s) 379, 468
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 4 cut(s) 121, 249, 387, 477
BssMI GATC 2 cut(s) 271, 277
BssT1I CCWWGG 2 cut(s) 121, 477
Bst2UI CCWGG 2 cut(s) 250, 388
Bst4CI ACNGT 3 cut(s) 163, 339, 547
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 6 cut(s) 64, 287, 354, 398, 438, 495
BstDSI CCRYGG 2 cut(s) 121, 477
BstF5I GGATG 6 cut(s) 109, 203, 313, 487, 538, 670
BstKTI GATC 2 cut(s) 274, 280
BstMAI GTCTC 3 cut(s) 152, 224, 386
BstMBI GATC 2 cut(s) 271, 277
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstNI CCWGG 2 cut(s) 250, 388
BstSCI CCNGG 2 cut(s) 248, 386
BstV1I GCAGC 3 cut(s) 421, 596, 619
BstV2I GAAGAC 2 cut(s) 261, 626
BsuRI GGCC 3 cut(s) 88, 139, 171
BtgI CCRYGG 2 cut(s) 121, 477
BtsCI GGATG 6 cut(s) 109, 203, 313, 487, 538, 670
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 195
CciI TCATGA 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 87, 442
CseI GACGC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 132
CviAII CATG 6 cut(s) 122, 236, 275, 478, 550, 689
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 6 cut(s) 64, 287, 354, 398, 438, 495
DpnI GATC 2 cut(s) 273, 279
DpnII GATC 2 cut(s) 271, 277
Eco130I CCWWGG 2 cut(s) 121, 477
Eco147I AGGCCT 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 248, 386
EcoT14I CCWWGG 2 cut(s) 121, 477
ErhI CCWWGG 2 cut(s) 121, 477
Esp3I CGTCTC 2 cut(s) 152, 224
FaeI CATG 6 cut(s) 125, 239, 278, 481, 553, 692
FaqI GGGAC 2 cut(s) 130, 573
FatI CATG 6 cut(s) 121, 235, 274, 477, 549, 688
Fnu4HI GCNGC 5 cut(s) 180, 410, 474, 585, 633
FokI GGATG 6 cut(s) 96, 190, 320, 494, 545, 677
Fsp4HI GCNGC 5 cut(s) 180, 410, 474, 585, 633
FspBI CTAG 1 cut(s) 129
GluI GCNGC 5 cut(s) 180, 410, 474, 585, 633
GsaI CCCAGC 1 cut(s) 349
HaeIII GGCC 3 cut(s) 88, 139, 171
HgaI GACGC 1 cut(s) 57
Hin1II CATG 6 cut(s) 125, 239, 278, 481, 553, 692
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HinfI GANTC 2 cut(s) 185, 394
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 2 cut(s) 157, 275
Hpy8I GTNNAC 1 cut(s) 597
Hpy99I CGWCG 2 cut(s) 73, 219
HpyAV CCTTC 4 cut(s) 150, 161, 210, 512
HpyCH4III ACNGT 3 cut(s) 163, 339, 547
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 6 cut(s) 8, 377, 409, 519, 627, 706
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 6 cut(s) 64, 287, 354, 398, 438, 495
HpySE526I ACGT 1 cut(s) 508
Hsp92II CATG 6 cut(s) 125, 239, 278, 481, 553, 692
Kzo9I GATC 2 cut(s) 271, 277
LmnI GCTCC 2 cut(s) 202, 336
Lsp1109I GCAGC 3 cut(s) 421, 596, 619
LweI GCATC 2 cut(s) 298, 596
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 508
MaeIII GTNAC 1 cut(s) 20
MalI GATC 2 cut(s) 273, 279
MboI GATC 2 cut(s) 271, 277
MboII GAAGA 2 cut(s) 266, 631
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 2 cut(s) 82, 92
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 8 cut(s) 117, 271, 363, 433, 571, 574, 616, 661
MroXI GAANNNNTTC 1 cut(s) 425
MslI CAYNNNNRTG 2 cut(s) 548, 554
MspA1I CMGCKG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 250, 388
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 250, 388
MwoI GCNNNNNNNGC 1 cut(s) 85
NcoI CCATGG 2 cut(s) 121, 477
NdeII GATC 2 cut(s) 271, 277
NlaIII CATG 6 cut(s) 125, 239, 278, 481, 553, 692
NlaIV GGNNCC 2 cut(s) 351, 604
NmuCI GTSAC 1 cut(s) 20
PagI TCATGA 1 cut(s) 274
PceI AGGCCT 1 cut(s) 139
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 340
PkrI GCNGC 5 cut(s) 181, 411, 475, 586, 634
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 248, 386
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 2 cut(s) 248, 386
PspN4I GGNNCC 2 cut(s) 351, 604
PspPI GGNCC 2 cut(s) 87, 442
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 2 cut(s) 548, 554
SatI GCNGC 5 cut(s) 180, 410, 474, 585, 633
Sau3AI GATC 2 cut(s) 271, 277
Sau96I GGNCC 2 cut(s) 87, 442
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 2 cut(s) 250, 388
SduI GDGCHC 1 cut(s) 501
SetI ASST 9 cut(s) 199, 333, 360, 439, 444, 461, 504, 511, 604
SfaNI GCATC 2 cut(s) 298, 596
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 2 cut(s) 548, 554
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 2 cut(s) 82, 92
SseBI AGGCCT 1 cut(s) 139
SsiI CCGC 3 cut(s) 180, 305, 474
SspI AATATT 1 cut(s) 37
SspMI CTAG 1 cut(s) 129
StuI AGGCCT 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 248, 386
StyI CCWWGG 2 cut(s) 121, 477
TaaI ACNGT 3 cut(s) 163, 339, 547
TaiI ACGT 1 cut(s) 511
TaqI TCGA 3 cut(s) 156, 280, 315
TasI AATT 2 cut(s) 82, 92
TatI WGTACW 1 cut(s) 131
TauI GCSGC 2 cut(s) 182, 476
TfiI GAWTC 1 cut(s) 185
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 3 cut(s) 409, 584, 632
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 3 cut(s) 17, 29, 192
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 1 cut(s) 384
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.