Rw5G000050

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010498
Physical Location & Seq
Reverse (-)
108851 .. 112044
3194 bp
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UTR
Exon/CDS
Intron
Rw5G000050.1

Sequence Viewer

Length: 552 bp
ATGAATGCACTTACCCTCTGTAACCATAACCATAATATTTGTATCAATAGCCACCATCATTTCTCAGAGTCGGCGAAGCGCAATTGGCCCAAATTGTTTTCAATTCATCGCAGAAACCTTCGGACAAGTACTGGGCCTTCGTCTCTGGTAATCGAGACGGTGGAAGGCCAAGTTGCCGCTGATTCTGAGCAAACTCCTTCATCTGTTGCTCGTCGTCTCATTCTGCTTCGTCATGCCAAGAGTTCCTGGGAAGACCCCTCGCTGAAAGATCATGATCGACCCCTGAGTGCAAAAGGACAAGCCGATGCTGTCGAAATCTCTCACAAGCTTCAACAGTTGGGCTGGATTCCTCAGCTTATTTTATCCAGCAATGCAGTGCGAACCAGGGAGACTCTTACCATAATGCAGCGACAAGTGAGGGGCTTCTTGGAAGCTGAGGTCCATTACTTTTCCAGCTTTTATTCCATTGCGGCCATGGATGGGCAGACTGCTGAGCACCTTCAACATATTATCTGTAACTATTCAAGGGATGACATACTCACCGTCATGTAA

Protein Analysis

183

Amino Acids

20.76

Weight (kDa)

7.87

Isoelectric Point (pI)

59.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 73 - 143 5.6e-07 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52155 AT3G52155 AT3G52155 AT3G52155
fragaria_vesca FvH4_3g00011
malus_domestica MD10G1342400.v1.1
prunus_persica Prupe.4G000400_v2.0.a1
pyrus_communis pycom10g29170
rosa_chinensis RchiOBHm_Chr5g0000011
rosa_laevigata RLG00000030832
rosa_multiflora Rmu_sc0007644.1_g000011
rosa_roxburghii Rroxscaffold_1G00076050
rosa_rugosa Rorug04G0380000 Rorug04G0380100 Rorug04G0380200 Rorug04G0380300
rosa_samantha Rh5AG000200 Rh5BG001400 Rh5CG000200 Rh5DG000200
rosa_wichuraiana Rw5G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 177, 470
AcoI YGGCCR 1 cut(s) 471
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 480
AgsI TTSAA 4 cut(s) 102, 332, 503, 525
AjnI CCWGG 2 cut(s) 245, 383
AluBI AGCT 4 cut(s) 328, 355, 434, 456
AluI AGCT 4 cut(s) 328, 355, 434, 456
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 4 cut(s) 147, 149, 221, 383
AoxI GGCC 4 cut(s) 86, 134, 166, 471
ApeKI GCWGC 1 cut(s) 406
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 3 cut(s) 87, 134, 439
AsuHPI GGTGA 1 cut(s) 532
AvaII GGWCC 1 cut(s) 439
BarI GAAGNNNNNNTAC 2 cut(s) 121, 153
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 498
BbvCI CCTCAGC 2 cut(s) 351, 435
BbvI GCAGC 1 cut(s) 418
BccI CCATC 2 cut(s) 63, 473
BciT130I CCWGG 2 cut(s) 247, 385
BcoDI GTCTC 4 cut(s) 147, 149, 221, 383
BisI GCNGC 3 cut(s) 177, 407, 471
BlpI GCTNAGC 1 cut(s) 492
BlsI GCNGC 3 cut(s) 178, 408, 472
BmcAI AGTACT 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 247, 385
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 87, 134, 439
BmrFI CCNGG 2 cut(s) 247, 385
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 295
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 258
Bpu10I CCTNAGC 2 cut(s) 351, 435
Bpu1102I GCTNAGC 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 246, 384, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 480
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 2 cut(s) 376, 465
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 2 cut(s) 247, 385
BseDI CCNNGG 3 cut(s) 246, 384, 474
BseGI GGATG 2 cut(s) 484, 535
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 480
BseMI GCAATG 2 cut(s) 376, 465
BseMII CTCAG 6 cut(s) 78, 177, 275, 365, 426, 483
BseNI ACTGG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 418
BshFI GGCC 4 cut(s) 88, 136, 168, 473
BsiHKAI GWGCWC 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 480
BsmAI GTCTC 4 cut(s) 147, 149, 221, 383
BsmBI CGTCTC 3 cut(s) 147, 149, 221
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 4 cut(s) 88, 136, 168, 473
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 2 cut(s) 268, 274
Bsp1720I GCTNAGC 1 cut(s) 492
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 177, 470
BspANI GGCC 4 cut(s) 88, 136, 168, 473
BspCNI CTCAG 6 cut(s) 77, 178, 276, 364, 427, 484
BspHI TCATGA 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 376, 465
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 3 cut(s) 246, 384, 474
BssMI GATC 2 cut(s) 268, 274
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 247, 385
Bst4CI ACNGT 3 cut(s) 160, 336, 544
BstDEI CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 2 cut(s) 484, 535
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 2 cut(s) 271, 277
BstMAI GTCTC 4 cut(s) 147, 149, 221, 383
BstMBI GATC 2 cut(s) 268, 274
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstNI CCWGG 2 cut(s) 247, 385
BstSCI CCNGG 2 cut(s) 245, 383
BstV1I GCAGC 1 cut(s) 418
BstV2I GAAGAC 1 cut(s) 258
BsuRI GGCC 4 cut(s) 88, 136, 168, 473
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 92
BtsCI GGATG 2 cut(s) 484, 535
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 381
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 87, 134, 439
Csp6I GTAC 1 cut(s) 129
CviAII CATG 4 cut(s) 233, 272, 475, 547
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
DpnI GATC 2 cut(s) 270, 276
DpnII GATC 2 cut(s) 268, 274
EaeI YGGCCR 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 245, 383
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 3 cut(s) 147, 149, 221
FaeI CATG 4 cut(s) 236, 275, 478, 550
FaiI YATR 9 cut(s) 27, 33, 234, 273, 401, 476, 507, 536, 548
FatI CATG 4 cut(s) 232, 271, 474, 546
Fnu4HI GCNGC 3 cut(s) 177, 407, 471
FokI GGATG 2 cut(s) 491, 542
Fsp4HI GCNGC 3 cut(s) 177, 407, 471
GlaI GCGC 1 cut(s) 80
GluI GCNGC 3 cut(s) 177, 407, 471
HaeIII GGCC 4 cut(s) 88, 136, 168, 473
HhaI GCGC 1 cut(s) 81
Hin1II CATG 4 cut(s) 236, 275, 478, 550
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 68, 182, 346, 391
HphI GGTGA 1 cut(s) 532
Hpy188I TCNGA 3 cut(s) 67, 123, 187
Hpy188III TCNNGA 2 cut(s) 154, 272
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 5 cut(s) 128, 147, 158, 207, 509
HpyCH4III ACNGT 3 cut(s) 160, 336, 544
HpyCH4V TGCA 4 cut(s) 8, 290, 374, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
Hsp92II CATG 4 cut(s) 236, 275, 478, 550
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 268, 274
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 295
MaeIII GTNAC 2 cut(s) 20, 515
MalI GATC 2 cut(s) 270, 276
MboI GATC 2 cut(s) 268, 274
MboII GAAGA 1 cut(s) 263
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 3 cut(s) 82, 92, 102
MlyI GAGTC 2 cut(s) 77, 385
MnlI CCTC 5 cut(s) 26, 268, 360, 411, 430
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 247, 385
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 247, 385
MwoI GCNNNNNNNGC 1 cut(s) 85
NcoI CCATGG 1 cut(s) 474
NdeII GATC 2 cut(s) 268, 274
NlaIII CATG 4 cut(s) 236, 275, 478, 550
PagI TCATGA 1 cut(s) 271
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 182, 346
PkrI GCNGC 3 cut(s) 178, 408, 472
PleI GAGTC 2 cut(s) 76, 385
PpsI GAGTC 2 cut(s) 76, 385
Psp6I CCWGG 2 cut(s) 245, 383
PspGI CCWGG 2 cut(s) 245, 383
PspPI GGNCC 3 cut(s) 87, 134, 439
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 1 cut(s) 545
SatI GCNGC 3 cut(s) 177, 407, 471
Sau3AI GATC 2 cut(s) 268, 274
Sau96I GGNCC 3 cut(s) 87, 134, 439
ScaI AGTACT 1 cut(s) 130
SchI GAGTC 2 cut(s) 77, 385
ScrFI CCNGG 2 cut(s) 247, 385
SduI GDGCHC 1 cut(s) 498
SetI ASST 7 cut(s) 120, 330, 357, 436, 441, 458, 501
SfaNI GCATC 1 cut(s) 295
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 3 cut(s) 82, 92, 102
SsiI CCGC 2 cut(s) 177, 470
SspI AATATT 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 245, 383
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 160, 336, 544
TaqI TCGA 3 cut(s) 153, 277, 312
TasI AATT 3 cut(s) 82, 92, 102
TatI WGTACW 1 cut(s) 128
TauI GCSGC 2 cut(s) 179, 473
TfiI GAWTC 2 cut(s) 182, 346
TscAI CASTG 1 cut(s) 381
TseI GCWGC 1 cut(s) 406
TspDTI ATGAA 3 cut(s) 17, 95, 189
TspRI CASTG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.