pycom10g29170

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010498
Physical Location & Seq
Reverse (-)
29569146 .. 29571242
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g29170.3

Sequence Viewer

Length: 747 bp
ATGAATGCGGTAGCTCTCTTTAACCATAACATTAATTTGAATATTCAGCCGCATAGTTTCTCTTTCCAACCGGCTGCGAATCCCAAGTGGCCCAGAGCCTCTTCAAGTTCAATCCGTCACAGAAACCCTAGCAGTGGTACTAGGCCTTCATCTTCATCTTTGGCAGTGGAAGTGGAGAGCAAAGCTGTTTCTGATACTGAACAAACTCCTCCTCCATCCGTTGCTCGTCGCCTAATTCTGCTTCGTCACGCCAACAGTTCCTGGGTAGACCCTTCGCTAAAAGATCATGATCGCCCCCTGAGTGAAAGAGGACAAGCCGATGCTGTCAAAGTCTCTCTCAAACTCCAAGAGTTGGGTTGGATTCCTCAGCTTATTTTATCTAGCAATGCAGTGCGAACTAGGGAGACTCTTACTATAATGCAGCAACAAGTAAGAGGCTTCTTGGAGGCTGAGGTTCATTATATTTCCAGCTTTTATTCCATCGCCGCCATGGATGGGCAGACAGCTGAGCACCTTCAACACATTATCTGTAACTATTCTAGGGACGACATACTCACTGTCATGTGCATGGGGCATAATAGGGGTTGGGAGGAGGCAGCCTCAATGTTCACTGGTGCCTCCATAGAACTCAAGACTTGCAATGCTGCTTTGCTTGAAACTACTGGGAAATCTTGGGATGAGGCATTTACTTTGGCAGGATTTGGGGGGTGGAAGCTGCAGGGCATTGTGAAACCTGTTAGACTATAG

Protein Analysis

249

Amino Acids

27.43

Weight (kDa)

7.82

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52155 AT3G52155 AT3G52155 AT3G52155
fragaria_vesca FvH4_3g00011
malus_domestica MD10G1342400.v1.1
prunus_persica Prupe.4G000400_v2.0.a1
pyrus_communis pycom10g29170
rosa_chinensis RchiOBHm_Chr5g0000011
rosa_laevigata RLG00000030832
rosa_multiflora Rmu_sc0007644.1_g000011
rosa_roxburghii Rroxscaffold_1G00076050
rosa_rugosa Rorug04G0380000 Rorug04G0380100 Rorug04G0380200 Rorug04G0380300
rosa_samantha Rh5AG000200 Rh5BG001400 Rh5CG000200 Rh5DG000200
rosa_wichuraiana Rw5G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 352
AccI GTMKAC 1 cut(s) 267
AciI CCGC 3 cut(s) 8, 50, 486
AfaI GTAC 1 cut(s) 139
AfiI CCNNNNNNNGG 3 cut(s) 134, 352, 495
AgsI TTSAA 5 cut(s) 40, 105, 111, 518, 656
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 6 cut(s) 14, 185, 370, 471, 506, 715
AluI AGCT 6 cut(s) 14, 185, 370, 471, 506, 715
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 2 cut(s) 337, 398
AlwNI CAGNNNCTG 1 cut(s) 261
AoxI GGCC 2 cut(s) 89, 143
ApeKI GCWGC 5 cut(s) 74, 421, 596, 644, 715
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 614
BarI GAAGNNNNNNTAC 2 cut(s) 130, 162
Bbv12I GWGCWC 1 cut(s) 513
BbvCI CCTCAGC 2 cut(s) 366, 450
BbvI GCAGC 5 cut(s) 61, 433, 608, 631, 702
BccI CCATC 3 cut(s) 223, 488, 488
BciT130I CCWGG 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 337, 398
BfaI CTAG 5 cut(s) 129, 141, 381, 399, 540
BfmI CTRYAG 2 cut(s) 716, 743
BisI GCNGC 7 cut(s) 50, 75, 422, 486, 597, 645, 716
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 7 cut(s) 51, 76, 423, 487, 598, 646, 717
Bme1390I CCNGG 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 262
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 672
BplI GAGNNNNNCTC 2 cut(s) 584, 616
Bpu10I CCTNAGC 2 cut(s) 366, 450
Bpu1102I GCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 261, 489
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 352, 495
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 2 cut(s) 616, 667
Bse3DI GCAATG 2 cut(s) 391, 646
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 261, 489
BseGI GGATG 3 cut(s) 215, 499, 682
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 3 cut(s) 134, 352, 495
BseMI GCAATG 2 cut(s) 391, 646
BseMII CTCAG 4 cut(s) 290, 380, 441, 498
BseNI ACTGG 2 cut(s) 616, 667
BseRI GAGGAG 3 cut(s) 198, 201, 605
BseXI GCAGC 5 cut(s) 61, 433, 608, 631, 702
BshFI GGCC 2 cut(s) 91, 145
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 513
BsiSI CCGG 1 cut(s) 71
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 3 cut(s) 134, 352, 495
BsmAI GTCTC 2 cut(s) 337, 398
BsmFI GGGAC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 91, 145
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 2 cut(s) 283, 289
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 3 cut(s) 8, 50, 486
BspANI GGCC 2 cut(s) 91, 145
BspCNI CTCAG 4 cut(s) 291, 379, 442, 499
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 391, 646
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 2 cut(s) 616, 667
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 261, 489
BssMI GATC 2 cut(s) 283, 289
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 257, 559
Bst6I CTCTTC 1 cut(s) 106
BstDEI CTNAG 4 cut(s) 299, 366, 450, 507
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 3 cut(s) 215, 499, 682
BstKTI GATC 2 cut(s) 286, 292
BstMAI GTCTC 2 cut(s) 337, 398
BstMBI GATC 2 cut(s) 283, 289
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 2 cut(s) 716, 743
BstV1I GCAGC 5 cut(s) 61, 433, 608, 631, 702
BsuRI GGCC 2 cut(s) 91, 145
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 3 cut(s) 215, 499, 682
BtsI GCAGTG 3 cut(s) 139, 171, 396
BtsIMutI CAGTG 5 cut(s) 139, 171, 396, 555, 609
CaiI CAGNNNCTG 1 cut(s) 261
CciI TCATGA 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 90
Csp6I GTAC 1 cut(s) 138
CviAII CATG 4 cut(s) 287, 490, 562, 568
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 4 cut(s) 299, 366, 450, 507
DpnI GATC 2 cut(s) 285, 291
DpnII GATC 2 cut(s) 283, 289
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 489
Eco147I AGGCCT 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 4 cut(s) 290, 493, 565, 571
FaqI GGGAC 1 cut(s) 557
FatI CATG 4 cut(s) 286, 489, 561, 567
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 7 cut(s) 50, 75, 422, 486, 597, 645, 716
FokI GGATG 3 cut(s) 202, 506, 689
Fsp4HI GCNGC 7 cut(s) 50, 75, 422, 486, 597, 645, 716
FspBI CTAG 5 cut(s) 129, 141, 381, 399, 540
GluI GCNGC 7 cut(s) 50, 75, 422, 486, 597, 645, 716
HaeIII GGCC 2 cut(s) 91, 145
HapII CCGG 1 cut(s) 71
Hin1II CATG 4 cut(s) 290, 493, 565, 571
HinfI GANTC 3 cut(s) 79, 361, 406
HpaII CCGG 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 268, 609
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 2 cut(s) 287, 631
Hpy8I GTNNAC 2 cut(s) 268, 609
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 3 cut(s) 156, 282, 524
HpyCH4III ACNGT 2 cut(s) 257, 559
HpyCH4V TGCA 5 cut(s) 389, 421, 567, 639, 718
HpyF3I CTNAG 4 cut(s) 299, 366, 450, 507
Hsp92II CATG 4 cut(s) 290, 493, 565, 571
Kzo9I GATC 2 cut(s) 283, 289
Lsp1109I GCAGC 5 cut(s) 61, 433, 608, 631, 702
LweI GCATC 1 cut(s) 310
MaeI CTAG 5 cut(s) 129, 141, 381, 399, 540
MaeIII GTNAC 3 cut(s) 116, 245, 530
MalI GATC 2 cut(s) 285, 291
MboI GATC 2 cut(s) 283, 289
MboII GAAGA 2 cut(s) 93, 144
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 2 cut(s) 34, 234
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 91, 338
MseI TTAA 2 cut(s) 21, 33
MslI CAYNNNNRTG 2 cut(s) 560, 566
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 262
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 262
NcoI CCATGG 1 cut(s) 489
NdeII GATC 2 cut(s) 283, 289
NlaIII CATG 4 cut(s) 290, 493, 565, 571
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 2 cut(s) 116, 245
PagI TCATGA 1 cut(s) 286
PceI AGGCCT 1 cut(s) 145
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 79, 361
PflMI CCANNNNNTGG 1 cut(s) 352
PkrI GCNGC 7 cut(s) 51, 76, 423, 487, 598, 646, 717
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PshBI ATTAAT 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 1 cut(s) 90
PstI CTGCAG 1 cut(s) 720
PstNI CAGNNNCTG 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
RseI CAYNNNNRTG 2 cut(s) 560, 566
SaqAI TTAA 2 cut(s) 21, 33
SatI GCNGC 7 cut(s) 50, 75, 422, 486, 597, 645, 716
Sau3AI GATC 2 cut(s) 283, 289
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 1 cut(s) 513
SetI ASST 9 cut(s) 16, 187, 372, 456, 473, 508, 516, 717, 736
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 2 cut(s) 716, 743
SmiMI CAYNNNNRTG 2 cut(s) 560, 566
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 2 cut(s) 34, 234
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 3 cut(s) 8, 50, 486
SspI AATATT 1 cut(s) 43
SspMI CTAG 5 cut(s) 129, 141, 381, 399, 540
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 2 cut(s) 257, 559
TasI AATT 2 cut(s) 34, 234
TauI GCSGC 2 cut(s) 52, 488
TfiI GAWTC 2 cut(s) 79, 361
Tru1I TTAA 2 cut(s) 21, 33
Tru9I TTAA 2 cut(s) 21, 33
TscAI CASTG 5 cut(s) 139, 171, 396, 562, 616
TseFI GTSAC 2 cut(s) 116, 245
TseI GCWGC 5 cut(s) 74, 421, 596, 644, 715
Tsp45I GTSAC 2 cut(s) 116, 245
TspDTI ATGAA 4 cut(s) 17, 138, 144, 446
TspGWI ACGGA 2 cut(s) 104, 208
TspRI CASTG 5 cut(s) 139, 171, 396, 562, 616
Van91I CCANNNNNTGG 1 cut(s) 352
VspI ATTAAT 1 cut(s) 33
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 5 cut(s) 129, 141, 381, 399, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.