Rh5CG000200

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010498
Physical Location & Seq
Reverse (-)
55839 .. 58842
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG000200.1

Sequence Viewer

Length: 753 bp
ATGAATGCACTTACCCTCTGTAACCATAACCATAATATTTGTATCAATAGCCACCATCATTTCTCAGCGTCGGCGAAGCGCAATTGGCCCAAATTGTTTTCAATTCATCCCAGAAACCTTCGTACAAGTACTGGGCCTTCGTCTCTGGTAATCGAGACGGTGGAAGGCCAAGTTGCCGCTGATTCTGAGCAAACTCCTTCATCTGTTGCTCGTCGTCTCATTCTGCTTCGTCATGCCAAGAGTTCCTGGGAAGACCCCTCGCTGAAAGATCATGATCGACCCCTGAGTGCAAAAGGACAAGCCGATGCTGTCGAAATCTCTCACAAGCTTCAACAGTTGGGCTGGATTCCTCAGCTTATTTTATCCAGCAATGCAGTGCGAACCAGGGAGACTCTTACCATAATGCAGCGACAAGTGAGGGGCTTCTTGGAAGCTGAGGTCCATTACTTTTCCAGCTTTTATTCCATTGCGGCCATGGATGGGCAGACTGCTGAGCACCTTCAACATATTATCTGTAACTATTCAAGGGATGACATACTCACCGTCATGTGTATGGGACATAATAGGGGTTGGGAGGAGGCAGCATCAATGTTGACAGGTGCCTCCATAGAACTGAAGACTTGCAACGCTGCTTTGCTTGAAGCTACTGGGAAATCTTGGGATGAGGTTAGTCTTCTTTCATTCCACTATTATTTTTTAACTGCTGTACTTCCCTTCTATATGCTTATATGTCAACTTAATGTGCATGTTTAA

Protein Analysis

250

Amino Acids

28.12

Weight (kDa)

6.72

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 73 - 140 1.2e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52155 AT3G52155 AT3G52155 AT3G52155
fragaria_vesca FvH4_3g00011
malus_domestica MD10G1342400.v1.1
prunus_persica Prupe.4G000400_v2.0.a1
pyrus_communis pycom10g29170
rosa_chinensis RchiOBHm_Chr5g0000011
rosa_laevigata RLG00000030832
rosa_multiflora Rmu_sc0007644.1_g000011
rosa_roxburghii Rroxscaffold_1G00076050
rosa_rugosa Rorug04G0380000 Rorug04G0380100 Rorug04G0380200 Rorug04G0380300
rosa_samantha Rh5AG000200 Rh5BG001400 Rh5CG000200 Rh5DG000200
rosa_wichuraiana Rw5G000050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 599
AciI CCGC 2 cut(s) 177, 470
AcoI YGGCCR 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 635
AfaI GTAC 3 cut(s) 124, 130, 708
AfiI CCNNNNNNNGG 1 cut(s) 480
AgsI TTSAA 5 cut(s) 102, 332, 503, 525, 641
AjnI CCWGG 2 cut(s) 245, 383
AluBI AGCT 5 cut(s) 328, 355, 434, 456, 644
AluI AGCT 5 cut(s) 328, 355, 434, 456, 644
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 4 cut(s) 147, 149, 221, 383
AoxI GGCC 4 cut(s) 86, 134, 166, 471
ApeKI GCWGC 3 cut(s) 406, 581, 629
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 3 cut(s) 87, 134, 439
AsuHPI GGTGA 1 cut(s) 532
AvaII GGWCC 1 cut(s) 439
BanI GGYRCC 1 cut(s) 599
BarI GAAGNNNNNNTAC 2 cut(s) 121, 153
BbsI GAAGAC 3 cut(s) 258, 623, 665
Bbv12I GWGCWC 1 cut(s) 498
BbvCI CCTCAGC 2 cut(s) 351, 435
BbvI GCAGC 3 cut(s) 418, 593, 616
BccI CCATC 2 cut(s) 63, 473
BciT130I CCWGG 2 cut(s) 247, 385
BcoDI GTCTC 4 cut(s) 147, 149, 221, 383
BisI GCNGC 5 cut(s) 177, 407, 471, 582, 630
BlpI GCTNAGC 1 cut(s) 492
BlsI GCNGC 5 cut(s) 178, 408, 472, 583, 631
BmcAI AGTACT 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 247, 385
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 87, 134, 439
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 2 cut(s) 247, 385
BmrI ACTGGG 2 cut(s) 141, 657
BmsI GCATC 2 cut(s) 295, 593
BmuI ACTGGG 2 cut(s) 141, 657
BpiI GAAGAC 3 cut(s) 258, 623, 665
Bpu10I CCTNAGC 2 cut(s) 351, 435
Bpu1102I GCTNAGC 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 246, 384, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 480
Bse1I ACTGG 2 cut(s) 136, 652
Bse3DI GCAATG 2 cut(s) 376, 465
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 2 cut(s) 247, 385
BseDI CCNNGG 3 cut(s) 246, 384, 474
BseGI GGATG 4 cut(s) 106, 484, 535, 667
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 480
BseMI GCAATG 2 cut(s) 376, 465
BseMII CTCAG 6 cut(s) 78, 177, 275, 365, 426, 483
BseNI ACTGG 2 cut(s) 136, 652
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 3 cut(s) 418, 593, 616
BshFI GGCC 4 cut(s) 88, 136, 168, 473
BshNI GGYRCC 1 cut(s) 599
BsiHKAI GWGCWC 1 cut(s) 498
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 1 cut(s) 480
BsmAI GTCTC 4 cut(s) 147, 149, 221, 383
BsmBI CGTCTC 3 cut(s) 147, 149, 221
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 4 cut(s) 88, 136, 168, 473
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 2 cut(s) 268, 274
Bsp1720I GCTNAGC 1 cut(s) 492
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 177, 470
BspANI GGCC 4 cut(s) 88, 136, 168, 473
BspCNI CTCAG 6 cut(s) 77, 178, 276, 364, 427, 484
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 599
BsrDI GCAATG 2 cut(s) 376, 465
BsrI ACTGG 2 cut(s) 136, 652
BssECI CCNNGG 3 cut(s) 246, 384, 474
BssMI GATC 2 cut(s) 268, 274
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 247, 385
Bst4CI ACNGT 3 cut(s) 160, 336, 544
BstDEI CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 4 cut(s) 106, 484, 535, 667
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 2 cut(s) 271, 277
BstMAI GTCTC 4 cut(s) 147, 149, 221, 383
BstMBI GATC 2 cut(s) 268, 274
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstNI CCWGG 2 cut(s) 247, 385
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 2 cut(s) 245, 383
BstV1I GCAGC 3 cut(s) 418, 593, 616
BstV2I GAAGAC 3 cut(s) 258, 623, 665
BsuRI GGCC 4 cut(s) 88, 136, 168, 473
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 4 cut(s) 106, 484, 535, 667
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 381
CciI TCATGA 1 cut(s) 271
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 87, 134, 439
CseI GACGC 1 cut(s) 57
Csp6I GTAC 3 cut(s) 123, 129, 707
CviAII CATG 5 cut(s) 233, 272, 475, 547, 746
CviQI GTAC 3 cut(s) 123, 129, 707
DdeI CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
DpnI GATC 2 cut(s) 270, 276
DpnII GATC 2 cut(s) 268, 274
EaeI YGGCCR 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 245, 383
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 3 cut(s) 147, 149, 221
FaeI CATG 5 cut(s) 236, 275, 478, 550, 749
FaqI GGGAC 1 cut(s) 570
FatI CATG 5 cut(s) 232, 271, 474, 546, 745
Fnu4HI GCNGC 5 cut(s) 177, 407, 471, 582, 630
FokI GGATG 4 cut(s) 93, 491, 542, 674
Fsp4HI GCNGC 5 cut(s) 177, 407, 471, 582, 630
GlaI GCGC 1 cut(s) 80
GluI GCNGC 5 cut(s) 177, 407, 471, 582, 630
HaeIII GGCC 4 cut(s) 88, 136, 168, 473
HgaI GACGC 1 cut(s) 57
HhaI GCGC 1 cut(s) 81
Hin1II CATG 5 cut(s) 236, 275, 478, 550, 749
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HincII GTYRAC 2 cut(s) 594, 734
HindII GTYRAC 2 cut(s) 594, 734
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 182, 346, 391
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 2 cut(s) 594, 734
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 2 cut(s) 154, 272
Hpy8I GTNNAC 2 cut(s) 594, 734
Hpy99I CGWCG 2 cut(s) 73, 216
HpyAV CCTTC 6 cut(s) 128, 147, 158, 207, 509, 724
HpyCH4III ACNGT 3 cut(s) 160, 336, 544
HpyCH4V TGCA 6 cut(s) 8, 290, 374, 406, 624, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 6 cut(s) 64, 186, 284, 351, 435, 492
Hsp92II CATG 5 cut(s) 236, 275, 478, 550, 749
HspAI GCGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 268, 274
Lsp1109I GCAGC 3 cut(s) 418, 593, 616
LweI GCATC 2 cut(s) 295, 593
MaeIII GTNAC 2 cut(s) 20, 515
MalI GATC 2 cut(s) 270, 276
MboI GATC 2 cut(s) 268, 274
MboII GAAGA 3 cut(s) 263, 628, 665
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 3 cut(s) 82, 92, 102
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 9 cut(s) 26, 268, 360, 411, 430, 568, 571, 613, 658
MseI TTAA 3 cut(s) 698, 738, 751
MslI CAYNNNNRTG 2 cut(s) 545, 551
MspA1I CMGCKG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 247, 385
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 247, 385
MwoI GCNNNNNNNGC 1 cut(s) 85
NcoI CCATGG 1 cut(s) 474
NdeII GATC 2 cut(s) 268, 274
NlaIII CATG 5 cut(s) 236, 275, 478, 550, 749
NlaIV GGNNCC 1 cut(s) 601
NspI RCATGY 1 cut(s) 749
PagI TCATGA 1 cut(s) 271
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 182, 346
PkrI GCNGC 5 cut(s) 178, 408, 472, 583, 631
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 245, 383
PspGI CCWGG 2 cut(s) 245, 383
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 3 cut(s) 87, 134, 439
RsaI GTAC 3 cut(s) 124, 130, 708
RsaNI GTAC 3 cut(s) 123, 129, 707
RseI CAYNNNNRTG 2 cut(s) 545, 551
SaqAI TTAA 3 cut(s) 698, 738, 751
SatI GCNGC 5 cut(s) 177, 407, 471, 582, 630
Sau3AI GATC 2 cut(s) 268, 274
Sau96I GGNCC 3 cut(s) 87, 134, 439
ScaI AGTACT 1 cut(s) 130
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 2 cut(s) 247, 385
SduI GDGCHC 1 cut(s) 498
SfaNI GCATC 2 cut(s) 295, 593
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 2 cut(s) 545, 551
Sse9I AATT 3 cut(s) 82, 92, 102
SsiI CCGC 2 cut(s) 177, 470
SspI AATATT 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 245, 383
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 160, 336, 544
TaqI TCGA 3 cut(s) 153, 277, 312
TasI AATT 3 cut(s) 82, 92, 102
TatI WGTACW 2 cut(s) 128, 706
TauI GCSGC 2 cut(s) 179, 473
TfiI GAWTC 2 cut(s) 182, 346
Tru1I TTAA 3 cut(s) 698, 738, 751
Tru9I TTAA 3 cut(s) 698, 738, 751
TscAI CASTG 1 cut(s) 381
TseI GCWGC 3 cut(s) 406, 581, 629
TspDTI ATGAA 4 cut(s) 17, 95, 189, 669
TspRI CASTG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 439
XceI RCATGY 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.