FvH4_3g00180

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014539
Physical Location & Seq
Forward (+)
108585 .. 111099
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00180.t1

Sequence Viewer

Length: 1434 bp
ATGCCGCATCCAGAACCTGAGCACAAACAACTTAATCCAATCAATCCAATAGAGGTTGATGATGATGACGGTGATGAGTTGATCTTGTCATTGTCGCCGGTGAAGCTCCAAAGGCTACCCCACCTCACCGACTATCTCTCACAACTCCCAACCCACAACAACCCACTCGAACACAACCCCTTCTACCACCCCTCCGACGGCTTCTACATAACCCCATCCGATGTCATCCTCCGCCAGATTGTCTACGACCTCTCCGCCGCCGGCGCCTCCTCTGGTCCCGCCTCAGCTTACCATCGCGCCGGCCCCCGACGGCACATATTCTTCGAGCCGTCGAGCATCAAGGCGGCTATAGTGACATGCGGTGGGCTGTGCCCGGGAATGAACACGGTGGTGAGAGAGCTGGTTCTTGCCCTCTGGGACCTGTACGGAGTCCGTCAGATTTTTGGTATCAAGGCTGGCTATCGTGGATTTCACTCTATGGACCCTATCCAGCTTGACCCAAAGCTCGTCCACAACTGGCACAAGAATGGCGGCACTGTCCTCGAGACCTCCCGAGGTGGTTTTGATCTCCATAAGATTGTCGATGCCATCCAAAATCGCGGCTATAACCAGGTGTACATCATAGGTGGAGACGGCACAATGCGTGGGGCAGTTGATATATTTCATGAGATTTGCAGGCGAAAACTGAAAATTGGGGTTACTGGGATTCCAAAAACAGTTGACAATGATGTTGGCATCATTGACAAATCTTTCGGATTCCAGACAGCAGTAGAAATGGCCCAGCAGGCAATAAGTGCAGGTCATGTGGAGGCTGAAAGTGCAGTGAATGGAATAGGCTTGGTCAAGTTGATGGGTCGAAGTACTGGCCACATTGCTCTGCACGCAACATTGAGCAGCCGAGATGTGGACTGCTGCCTAATCCCTGAAACTGAGTTTTACCTGGAAGGAAAAGGAGGTTTATTTGAATTCATGGAGCAGCGACTGAGGGAGAATGGACACGCAGTGTTAGTTGTGGCTGAAGGAGCGGGACAGGATATAATTCCGAGGAACGAGGCTCAGAAACAAGAGAGGGATGAATCTGGGAACCCAGTGTTCCTAGATGTTGGCCAATGGTTGAAGTCGGAGCTCAAGAATTGGTGGAGCAGGGATCACCCCAACGAGCTGGTCACAGTCAAGTACATAGACCCTACATACATGATCCGAGCCGTCCCTGCAAATGCTACAGATAACTTGTACTGTACACTTCTTGCCCATTCAGCTATTCATGGAGTGATGGCTGGTTACACTGGATTCGTCCCTGGCCCCATCAATGGCAACTATGCTTACATTCCAGTTGAAGAGGTGGCGAAGGCTAAGAATGTGGTCAACACAAGAGACCACAAATGGGCGTGGGTAAGATCTGTCACCAACCAGCCTGATTTTAACAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

478

Amino Acids

52.81

Weight (kDa)

6.01

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 115 - 422 6.8e-54 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 1025
AccI GTMKAC 1 cut(s) 243
AccII CGCG 2 cut(s) 297, 600
AclWI GGATC 2 cut(s) 1155, 1192
AcoI YGGCCR 2 cut(s) 865, 1105
AcsI RAATTY 1 cut(s) 965
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 264
AdeI CACNNNGTG 1 cut(s) 1003
AfaI GTAC 6 cut(s) 425, 617, 862, 1178, 1235, 1240
AfiI CCNNNNNNNGG 4 cut(s) 197, 904, 1310, 1384
AgsI TTSAA 3 cut(s) 965, 1117, 1337
AjnI CCWGG 3 cut(s) 609, 939, 1297
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 8 cut(s) 106, 287, 400, 493, 505, 1126, 1162, 1259
AluI AGCT 8 cut(s) 106, 287, 400, 493, 505, 1126, 1162, 1259
Alw21I GWGCWC 2 cut(s) 24, 1128
Alw26I GTCTC 3 cut(s) 539, 624, 1368
AlwI GGATC 2 cut(s) 1155, 1192
AlwNI CAGNNNCTG 2 cut(s) 17, 982
Ama87I CYCGRG 3 cut(s) 373, 542, 552
AoxI GGCC 5 cut(s) 301, 777, 865, 1105, 1300
ApeKI GCWGC 3 cut(s) 894, 912, 976
ApoI RAATTY 1 cut(s) 965
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AspLEI GCGC 2 cut(s) 266, 299
AspS9I GGNCC 6 cut(s) 275, 302, 418, 481, 778, 1301
AsuC2I CCSGG 2 cut(s) 374, 375
AsuHPI GGTGA 6 cut(s) 83, 112, 118, 403, 1142, 1396
AvaI CYCGRG 3 cut(s) 373, 542, 552
AvaII GGWCC 3 cut(s) 275, 418, 481
BaeGI GKGCMC 1 cut(s) 374
BalI TGGCCA 2 cut(s) 867, 1107
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 1128
Bbv12I GWGCWC 2 cut(s) 24, 1128
BbvCI CCTCAGC 1 cut(s) 283
BbvI GCAGC 3 cut(s) 899, 906, 988
BccI CCATC 6 cut(s) 223, 300, 596, 844, 1267, 1313
BceAI ACGGC 5 cut(s) 214, 313, 326, 649, 1190
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 3 cut(s) 611, 941, 1299
BcnI CCSGG 2 cut(s) 374, 375
BcoDI GTCTC 3 cut(s) 539, 624, 1368
BfaI CTAG 1 cut(s) 1097
BfmI CTRYAG 2 cut(s) 348, 1221
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 788
BglI GCCNNNNNGGC 1 cut(s) 785
BglII AGATCT 1 cut(s) 1397
BisI GCNGC 8 cut(s) 5, 258, 345, 532, 601, 895, 913, 977
BlsI GCNGC 8 cut(s) 6, 259, 346, 533, 602, 896, 914, 978
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 5 cut(s) 374, 375, 611, 941, 1299
Bme18I GGWCC 3 cut(s) 275, 418, 481
BmeT110I CYCGRG 3 cut(s) 373, 542, 552
BmgT120I GGNCC 6 cut(s) 275, 302, 418, 481, 778, 1301
BmiI GGNNCC 7 cut(s) 265, 277, 304, 419, 483, 1085, 1303
BmrFI CCNGG 5 cut(s) 374, 375, 611, 941, 1299
BmrI ACTGGG 2 cut(s) 711, 1082
BmsI GCATC 4 cut(s) 16, 345, 574, 744
BmuI ACTGGG 2 cut(s) 711, 1082
Bpu10I CCTNAGC 2 cut(s) 18, 283
BpuEI CTTGAG 1 cut(s) 1112
BpuMI CCSGG 2 cut(s) 374, 375
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 2 cut(s) 539, 1368
BsaJI CCNNGG 4 cut(s) 373, 553, 1043, 1297
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 236, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 904, 1310, 1384
Bse118I RCCGGY 3 cut(s) 97, 260, 299
Bse1I ACTGG 6 cut(s) 521, 706, 868, 1088, 1291, 1331
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 3 cut(s) 611, 941, 1299
BseDI CCNNGG 4 cut(s) 373, 553, 1043, 1297
BseGI GGATG 5 cut(s) 7, 215, 225, 588, 1078
BseLI CCNNNNNNNGG 4 cut(s) 197, 904, 1310, 1384
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 5 cut(s) 9, 297, 921, 974, 1070
BseNI ACTGG 6 cut(s) 521, 706, 868, 1088, 1291, 1331
BseRI GAGGAG 1 cut(s) 259
BseSI GKGCMC 1 cut(s) 374
BseXI GCAGC 3 cut(s) 899, 906, 988
BseYI CCCAGC 1 cut(s) 780
BsgI GTGCAG 3 cut(s) 816, 840, 863
Bsh1236I CGCG 2 cut(s) 297, 600
BshFI GGCC 5 cut(s) 303, 779, 867, 1107, 1302
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 24, 1128
BsiHKCI CYCGRG 3 cut(s) 373, 542, 552
BsiSI CCGG 4 cut(s) 98, 261, 300, 374
BslFI GGGAC 5 cut(s) 261, 431, 1041, 1193, 1280
BslI CCNNNNNNNGG 4 cut(s) 197, 904, 1310, 1384
BsmAI GTCTC 3 cut(s) 539, 624, 1368
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 5 cut(s) 261, 431, 1041, 1193, 1280
BsnI GGCC 5 cut(s) 303, 779, 867, 1107, 1302
Bso31I GGTCTC 2 cut(s) 539, 1368
BsoBI CYCGRG 3 cut(s) 373, 542, 552
Bsp1286I GDGCHC 3 cut(s) 24, 374, 1128
Bsp1407I TGTACA 2 cut(s) 615, 1238
Bsp143I GATC 5 cut(s) 81, 565, 1147, 1197, 1397
BspANI GGCC 5 cut(s) 303, 779, 867, 1107, 1302
BspCNI CTCAG 5 cut(s) 10, 296, 922, 975, 1069
BspFNI CGCG 2 cut(s) 297, 600
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 7 cut(s) 265, 277, 304, 419, 483, 1085, 1303
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 1155, 1192
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 2 cut(s) 539, 1368
BsrBI CCGCTC 1 cut(s) 1025
BsrDI GCAATG 1 cut(s) 870
BsrFI RCCGGY 3 cut(s) 97, 260, 299
BsrGI TGTACA 2 cut(s) 615, 1238
BsrI ACTGG 6 cut(s) 521, 706, 868, 1088, 1291, 1331
BssAI RCCGGY 3 cut(s) 97, 260, 299
BssECI CCNNGG 4 cut(s) 373, 553, 1043, 1297
BssMI GATC 5 cut(s) 81, 565, 1147, 1197, 1397
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 611, 941, 1299
Bst4CI ACNGT 6 cut(s) 71, 388, 538, 718, 1171, 1238
Bst6I CTCTTC 1 cut(s) 1332
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 2 cut(s) 615, 1238
BstC8I GCNNGC 6 cut(s) 262, 301, 457, 677, 786, 882
BstDEI CTNAG 6 cut(s) 18, 283, 930, 983, 1056, 1353
BstF5I GGATG 5 cut(s) 7, 215, 225, 588, 1078
BstFNI CGCG 2 cut(s) 297, 600
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 2 cut(s) 266, 299
BstKTI GATC 5 cut(s) 84, 568, 1150, 1200, 1400
BstMAI GTCTC 3 cut(s) 539, 624, 1368
BstMBI GATC 5 cut(s) 81, 565, 1147, 1197, 1397
BstNI CCWGG 3 cut(s) 611, 941, 1299
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 5 cut(s) 372, 373, 609, 939, 1297
BstSFI CTRYAG 2 cut(s) 348, 1221
BstSLI GKGCMC 1 cut(s) 374
BstUI CGCG 2 cut(s) 297, 600
BstV1I GCAGC 3 cut(s) 899, 906, 988
BstX2I RGATCY 1 cut(s) 1397
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1397
BsuRI GGCC 5 cut(s) 303, 779, 867, 1107, 1302
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 7, 215, 225, 588, 1078
BtsI GCAGTG 2 cut(s) 828, 1008
BtsIMutI CAGTG 5 cut(s) 534, 828, 1008, 1095, 1284
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 6 cut(s) 262, 301, 457, 677, 786, 882
CaiI CAGNNNCTG 2 cut(s) 17, 982
CciI TCATGA 1 cut(s) 664
CfoI GCGC 2 cut(s) 266, 299
Cfr10I RCCGGY 3 cut(s) 97, 260, 299
Cfr13I GGNCC 6 cut(s) 275, 302, 418, 481, 778, 1301
Cfr9I CCCGGG 1 cut(s) 373
CsiI ACCWGGT 1 cut(s) 609
Csp6I GTAC 6 cut(s) 424, 616, 861, 1177, 1234, 1239
CviAII CATG 6 cut(s) 357, 665, 803, 970, 1195, 1265
CviQI GTAC 6 cut(s) 424, 616, 861, 1177, 1234, 1239
DdeI CTNAG 6 cut(s) 18, 283, 930, 983, 1056, 1353
DinI GGCGCC 1 cut(s) 265
DpnI GATC 5 cut(s) 83, 567, 1149, 1199, 1399
DpnII GATC 5 cut(s) 81, 565, 1147, 1197, 1397
DraIII CACNNNGTG 1 cut(s) 1003
EaeI YGGCCR 2 cut(s) 865, 1105
Eam1104I CTCTTC 1 cut(s) 1332
EarI CTCTTC 1 cut(s) 1332
EciI GGCGGA 2 cut(s) 221, 244
Ecl136II GAGCTC 1 cut(s) 1126
Eco24I GRGCYC 1 cut(s) 1128
Eco31I GGTCTC 2 cut(s) 539, 1368
Eco47I GGWCC 3 cut(s) 275, 418, 481
Eco53kI GAGCTC 1 cut(s) 1126
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 3 cut(s) 373, 542, 552
EcoICRI GAGCTC 1 cut(s) 1126
EcoO109I RGGNCCY 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 609, 939, 1297
EcoT38I GRGCYC 1 cut(s) 1128
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 624
FaeI CATG 6 cut(s) 360, 668, 806, 973, 1198, 1268
FaqI GGGAC 5 cut(s) 261, 431, 1041, 1193, 1280
FatI CATG 6 cut(s) 356, 664, 802, 969, 1194, 1264
FauI CCCGC 2 cut(s) 286, 1018
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 8 cut(s) 5, 258, 345, 532, 601, 895, 913, 977
FokI GGATG 4 cut(s) 202, 212, 575, 1085
FriOI GRGCYC 1 cut(s) 1128
Fsp4HI GCNGC 8 cut(s) 5, 258, 345, 532, 601, 895, 913, 977
FspBI CTAG 1 cut(s) 1097
GlaI GCGC 2 cut(s) 265, 298
GluI GCNGC 8 cut(s) 5, 258, 345, 532, 601, 895, 913, 977
GsaI CCCAGC 1 cut(s) 784
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 5 cut(s) 303, 779, 867, 1107, 1302
HapII CCGG 4 cut(s) 98, 261, 300, 374
HhaI GCGC 2 cut(s) 266, 299
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 6 cut(s) 360, 668, 806, 973, 1198, 1268
Hin6I GCGC 2 cut(s) 264, 297
HinP1I GCGC 2 cut(s) 264, 297
HincII GTYRAC 2 cut(s) 721, 1366
HindII GTYRAC 2 cut(s) 721, 1366
HinfI GANTC 5 cut(s) 429, 706, 756, 1076, 1290
HpaII CCGG 4 cut(s) 98, 261, 300, 374
HphI GGTGA 6 cut(s) 83, 112, 118, 403, 1142, 1396
Hpy166II GTNNAC 7 cut(s) 244, 511, 616, 721, 907, 1241, 1366
Hpy188I TCNGA 8 cut(s) 196, 220, 438, 755, 1044, 1059, 1123, 1202
Hpy188III TCNNGA 6 cut(s) 11, 544, 552, 665, 760, 1129
Hpy8I GTNNAC 7 cut(s) 244, 511, 616, 721, 907, 1241, 1366
Hpy99I CGWCG 3 cut(s) 200, 312, 334
HpyAV CCTTC 4 cut(s) 190, 938, 1013, 1342
HpyCH4III ACNGT 6 cut(s) 71, 388, 538, 718, 1171, 1238
HpyCH4V TGCA 5 cut(s) 675, 797, 821, 880, 1214
HpyF3I CTNAG 6 cut(s) 18, 283, 930, 983, 1056, 1353
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 6 cut(s) 360, 668, 806, 973, 1198, 1268
HspAI GCGC 2 cut(s) 264, 297
KasI GGCGCC 1 cut(s) 263
KroI GCCGGC 2 cut(s) 260, 299
KroNI GCCGGC 2 cut(s) 262, 301
Kzo9I GATC 5 cut(s) 81, 565, 1147, 1197, 1397
LmnI GCTCC 5 cut(s) 111, 973, 1022, 1123, 1140
Lsp1109I GCAGC 3 cut(s) 899, 906, 988
LweI GCATC 4 cut(s) 16, 345, 574, 744
MabI ACCWGGT 1 cut(s) 609
MaeI CTAG 1 cut(s) 1097
MaeIII GTNAC 5 cut(s) 352, 697, 1165, 1280, 1402
MalI GATC 5 cut(s) 83, 567, 1149, 1199, 1399
MbiI CCGCTC 1 cut(s) 1025
MboI GATC 5 cut(s) 81, 565, 1147, 1197, 1397
MboII GAAGA 2 cut(s) 313, 1349
MflI RGATCY 1 cut(s) 1397
MhlI GDGCHC 3 cut(s) 24, 374, 1128
MlsI TGGCCA 2 cut(s) 867, 1107
MluCI AATT 4 cut(s) 690, 965, 1038, 1132
MluNI TGGCCA 2 cut(s) 867, 1107
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 438
MmeI TCCRAC 2 cut(s) 219, 1101
Mox20I TGGCCA 2 cut(s) 867, 1107
MreI CGCCGGCG 1 cut(s) 260
MroNI GCCGGC 2 cut(s) 260, 299
MscI TGGCCA 2 cut(s) 867, 1107
MseI TTAA 2 cut(s) 33, 1422
MslI CAYNNNNRTG 2 cut(s) 389, 525
Msp20I TGGCCA 2 cut(s) 867, 1107
MspI CCGG 4 cut(s) 98, 261, 300, 374
MspR9I CCNGG 5 cut(s) 374, 375, 611, 941, 1299
MvaI CCWGG 3 cut(s) 611, 941, 1299
MvnI CGCG 2 cut(s) 297, 600
NaeI GCCGGC 2 cut(s) 262, 301
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 374, 375
NdeII GATC 5 cut(s) 81, 565, 1147, 1197, 1397
NgoMIV GCCGGC 2 cut(s) 260, 299
NlaIII CATG 6 cut(s) 360, 668, 806, 973, 1198, 1268
NlaIV GGNNCC 7 cut(s) 265, 277, 304, 419, 483, 1085, 1303
NmeAIII GCCGAG 1 cut(s) 923
NmuCI GTSAC 3 cut(s) 352, 1165, 1402
NspI RCATGY 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 542
PagI TCATGA 1 cut(s) 664
PdiI GCCGGC 2 cut(s) 262, 301
PfeI GAWTC 4 cut(s) 706, 756, 1076, 1290
PkrI GCNGC 8 cut(s) 6, 259, 346, 533, 602, 896, 914, 978
PleI GAGTC 1 cut(s) 437
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 437
PpuMI RGGWCCY 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 3 cut(s) 609, 939, 1297
PspFI CCCAGC 1 cut(s) 780
PspGI CCWGG 3 cut(s) 609, 939, 1297
PspN4I GGNNCC 7 cut(s) 265, 277, 304, 419, 483, 1085, 1303
PspPI GGNCC 6 cut(s) 275, 302, 418, 481, 778, 1301
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 2 cut(s) 17, 982
PsuI RGATCY 1 cut(s) 1397
RsaI GTAC 6 cut(s) 425, 617, 862, 1178, 1235, 1240
RsaNI GTAC 6 cut(s) 424, 616, 861, 1177, 1234, 1239
RseI CAYNNNNRTG 2 cut(s) 389, 525
SacI GAGCTC 1 cut(s) 1128
SaqAI TTAA 2 cut(s) 33, 1422
SatI GCNGC 8 cut(s) 5, 258, 345, 532, 601, 895, 913, 977
Sau3AI GATC 5 cut(s) 81, 565, 1147, 1197, 1397
Sau96I GGNCC 6 cut(s) 275, 302, 418, 481, 778, 1301
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 1 cut(s) 438
ScrFI CCNGG 5 cut(s) 374, 375, 611, 941, 1299
SduI GDGCHC 3 cut(s) 24, 374, 1128
SexAI ACCWGGT 1 cut(s) 609
SfaNI GCATC 4 cut(s) 16, 345, 574, 744
SfcI CTRYAG 2 cut(s) 348, 1221
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 542
SgrAI CRCCGGYG 2 cut(s) 97, 260
SinI GGWCC 3 cut(s) 275, 418, 481
SlaI CTCGAG 1 cut(s) 542
SmaI CCCGGG 1 cut(s) 375
SmiMI CAYNNNNRTG 2 cut(s) 389, 525
SmlI CTYRAG 2 cut(s) 542, 1127
SmoI CTYRAG 2 cut(s) 542, 1127
Sse9I AATT 4 cut(s) 690, 965, 1038, 1132
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 1097
SstI GAGCTC 1 cut(s) 1128
StyD4I CCNGG 5 cut(s) 372, 373, 609, 939, 1297
TaaI ACNGT 6 cut(s) 71, 388, 538, 718, 1171, 1238
TaqI TCGA 6 cut(s) 168, 324, 332, 543, 582, 856
TasI AATT 4 cut(s) 690, 965, 1038, 1132
TatI WGTACW 5 cut(s) 615, 860, 1176, 1233, 1238
TauI GCSGC 5 cut(s) 7, 260, 347, 534, 603
TfiI GAWTC 4 cut(s) 706, 756, 1076, 1290
Tru1I TTAA 2 cut(s) 33, 1422
Tru9I TTAA 2 cut(s) 33, 1422
TscAI CASTG 5 cut(s) 541, 828, 1008, 1095, 1291
TseFI GTSAC 3 cut(s) 352, 1165, 1402
TseI GCWGC 3 cut(s) 894, 912, 976
Tsp45I GTSAC 3 cut(s) 352, 1165, 1402
TspDTI ATGAA 5 cut(s) 395, 653, 958, 1089, 1253
TspGWI ACGGA 2 cut(s) 422, 441
TspMI CCCGGG 1 cut(s) 373
TspRI CASTG 5 cut(s) 541, 828, 1008, 1095, 1291
VpaK11BI GGWCC 3 cut(s) 275, 418, 481
XapI RAATTY 1 cut(s) 965
XceI RCATGY 1 cut(s) 360
XhoI CTCGAG 1 cut(s) 542
XmaI CCCGGG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 1097
ZrmI AGTACT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.