Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G47810 |
| fragaria_vesca | FvH4_3g00180 |
| malus_domestica | MD05G1363600.v1.1 |
| prunus_persica | Prupe.4G002000_v2.0.a1 |
| pyrus_communis | pycom05g32980 |
| rosa_chinensis | RchiOBHm_Chr5g0000241 |
| rosa_laevigata | RLG00000030852 |
| rosa_multiflora | Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00075860 |
| rosa_rugosa | Rorug04G0382700 |
| rosa_samantha | Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000 |
| rosa_wichuraiana | Rw5G000220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 476 |
| AatII | GACGTC | 1 cut(s) | 1278 |
| Acc16I | TGCGCA | 1 cut(s) | 1425 |
| Acc36I | ACCTGC | 1 cut(s) | 476 |
| AccB1I | GGYRCC | 1 cut(s) | 650 |
| AccI | GTMKAC | 2 cut(s) | 318, 1457 |
| AccII | CGCG | 3 cut(s) | 408, 717, 1377 |
| AciI | CCGC | 9 cut(s) | 8, 195, 307, 406, 456, 471, 717, 767, 1233 |
| AclWI | GGATC | 1 cut(s) | 1352 |
| AcsI | RAATTY | 3 cut(s) | 549, 1044, 1082 |
| AcyI | GRCGYC | 2 cut(s) | 421, 1275 |
| AfaI | GTAC | 4 cut(s) | 734, 979, 1352, 1409 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 484, 1021, 1213, 1484, 1558 |
| AgsI | TTSAA | 4 cut(s) | 203, 239, 689, 1511 |
| AhlI | ACTAGT | 1 cut(s) | 158 |
| AjiI | CACGTC | 1 cut(s) | 1021 |
| AjnI | CCWGG | 4 cut(s) | 435, 1056, 1269, 1562 |
| AluBI | AGCT | 9 cut(s) | 112, 121, 511, 622, 730, 917, 1300, 1336, 1608 |
| AluI | AGCT | 9 cut(s) | 112, 121, 511, 622, 730, 917, 1300, 1336, 1608 |
| Alw21I | GWGCWC | 3 cut(s) | 513, 1326, 1338 |
| Alw26I | GTCTC | 2 cut(s) | 741, 1542 |
| AlwI | GGATC | 1 cut(s) | 1352 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 818 |
| Ama87I | CYCGRG | 1 cut(s) | 484 |
| AoxI | GGCC | 8 cut(s) | 412, 453, 475, 487, 520, 1230, 1474, 1560 |
| ApeKI | GCWGC | 9 cut(s) | 210, 213, 216, 219, 395, 898, 914, 1011, 1029 |
| ApoI | RAATTY | 3 cut(s) | 549, 1044, 1082 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 851, 883, 967, 999 |
| AspLEI | GCGC | 4 cut(s) | 410, 898, 1379, 1426 |
| AspS9I | GGNCC | 7 cut(s) | 10, 413, 487, 529, 1230, 1475, 1560 |
| AsuC2I | CCSGG | 2 cut(s) | 485, 486 |
| AsuHPI | GGTGA | 3 cut(s) | 457, 970, 1570 |
| AsuII | TTCGAA | 1 cut(s) | 1080 |
| AvaI | CYCGRG | 1 cut(s) | 484 |
| AvaII | GGWCC | 2 cut(s) | 10, 529 |
| BanI | GGYRCC | 1 cut(s) | 650 |
| BanII | GRGCYC | 2 cut(s) | 513, 1338 |
| BbsI | GAAGAC | 1 cut(s) | 246 |
| Bbv12I | GWGCWC | 3 cut(s) | 513, 1326, 1338 |
| BbvCI | CCTCAGC | 1 cut(s) | 1134 |
| BbvI | GCAGC | 9 cut(s) | 222, 225, 228, 231, 382, 910, 926, 1016, 1023 |
| BccI | CCATC | 3 cut(s) | 690, 713, 1162 |
| BceAI | ACGGC | 3 cut(s) | 766, 999, 1364 |
| BciT130I | CCWGG | 4 cut(s) | 437, 1058, 1271, 1564 |
| BcnI | CCSGG | 2 cut(s) | 485, 486 |
| BcoDI | GTCTC | 2 cut(s) | 741, 1542 |
| BcuI | ACTAGT | 1 cut(s) | 158 |
| BfaI | CTAG | 4 cut(s) | 159, 339, 1545, 1609 |
| BfuAI | ACCTGC | 1 cut(s) | 476 |
| Bme1390I | CCNGG | 6 cut(s) | 437, 485, 486, 1058, 1271, 1564 |
| Bme18I | GGWCC | 2 cut(s) | 10, 529 |
| BmeT110I | CYCGRG | 1 cut(s) | 484 |
| BmgBI | CACGTC | 1 cut(s) | 1021 |
| BmgT120I | GGNCC | 7 cut(s) | 10, 413, 487, 529, 1230, 1475, 1560 |
| BmiI | GGNNCC | 8 cut(s) | 12, 57, 530, 600, 652, 1141, 1259, 1477 |
| BmrFI | CCNGG | 6 cut(s) | 437, 485, 486, 1058, 1271, 1564 |
| BmrI | ACTGGG | 3 cut(s) | 828, 1256, 1470 |
| BmsI | GCATC | 4 cut(s) | 376, 691, 747, 1186 |
| BmuI | ACTGGG | 3 cut(s) | 828, 1256, 1470 |
| BpiI | GAAGAC | 1 cut(s) | 246 |
| BpmI | CTGGAG | 2 cut(s) | 644, 652 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1134 |
| Bpu14I | TTCGAA | 1 cut(s) | 1080 |
| BpuEI | CTTGAG | 2 cut(s) | 1109, 1274 |
| BpuMI | CCSGG | 2 cut(s) | 485, 486 |
| BsaBI | GATNNNNATC | 1 cut(s) | 171 |
| BsaHI | GRCGYC | 2 cut(s) | 421, 1275 |
| BsaI | GGTCTC | 1 cut(s) | 1542 |
| BsaJI | CCNNGG | 5 cut(s) | 271, 484, 574, 588, 1563 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 245, 275 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 484, 1021, 1213, 1484, 1558 |
| Bse1I | ACTGG | 4 cut(s) | 638, 823, 1262, 1465 |
| Bse3DI | GCAATG | 1 cut(s) | 1289 |
| Bse8I | GATNNNNATC | 1 cut(s) | 171 |
| BseBI | CCWGG | 4 cut(s) | 437, 1058, 1271, 1564 |
| BseDI | CCNNGG | 5 cut(s) | 271, 484, 574, 588, 1563 |
| BseGI | GGATG | 3 cut(s) | 705, 1173, 1198 |
| BseJI | GATNNNNATC | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 484, 1021, 1213, 1484, 1558 |
| BseMI | GCAATG | 1 cut(s) | 1289 |
| BseMII | CTCAG | 3 cut(s) | 921, 1125, 1214 |
| BseNI | ACTGG | 4 cut(s) | 638, 823, 1262, 1465 |
| BseRI | GAGGAG | 3 cut(s) | 1174, 1177, 1330 |
| BseXI | GCAGC | 9 cut(s) | 222, 225, 228, 231, 382, 910, 926, 1016, 1023 |
| BsgI | GTGCAG | 3 cut(s) | 933, 957, 1396 |
| Bsh1236I | CGCG | 3 cut(s) | 408, 717, 1377 |
| BshFI | GGCC | 8 cut(s) | 414, 455, 477, 489, 522, 1232, 1476, 1562 |
| BshNI | GGYRCC | 1 cut(s) | 650 |
| BsiHKAI | GWGCWC | 3 cut(s) | 513, 1326, 1338 |
| BsiHKCI | CYCGRG | 1 cut(s) | 484 |
| BsiSI | CCGG | 2 cut(s) | 485, 1143 |
| BslFI | GGGAC | 2 cut(s) | 542, 1367 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 484, 1021, 1213, 1484, 1558 |
| BsmAI | GTCTC | 2 cut(s) | 741, 1542 |
| BsmBI | CGTCTC | 1 cut(s) | 741 |
| BsmFI | GGGAC | 2 cut(s) | 542, 1367 |
| BsmI | GAATGC | 1 cut(s) | 1426 |
| BsnI | GGCC | 8 cut(s) | 414, 455, 477, 489, 522, 1232, 1476, 1562 |
| Bso31I | GGTCTC | 1 cut(s) | 1542 |
| BsoBI | CYCGRG | 1 cut(s) | 484 |
| Bsp119I | TTCGAA | 1 cut(s) | 1080 |
| Bsp1286I | GDGCHC | 3 cut(s) | 513, 1326, 1338 |
| Bsp1407I | TGTACA | 1 cut(s) | 732 |
| Bsp143I | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| Bsp19I | CCATGG | 1 cut(s) | 271 |
| BspACI | CCGC | 9 cut(s) | 8, 195, 307, 406, 456, 471, 717, 767, 1233 |
| BspANI | GGCC | 8 cut(s) | 414, 455, 477, 489, 522, 1232, 1476, 1562 |
| BspCNI | CTCAG | 3 cut(s) | 922, 1126, 1213 |
| BspFNI | CGCG | 3 cut(s) | 408, 717, 1377 |
| BspHI | TCATGA | 1 cut(s) | 781 |
| BspLI | GGNNCC | 8 cut(s) | 12, 57, 530, 600, 652, 1141, 1259, 1477 |
| BspMI | ACCTGC | 1 cut(s) | 476 |
| BspPI | GGATC | 1 cut(s) | 1352 |
| BspQI | GCTCTTC | 1 cut(s) | 1343 |
| BspT104I | TTCGAA | 1 cut(s) | 1080 |
| BspT107I | GGYRCC | 1 cut(s) | 650 |
| BspTNI | GGTCTC | 1 cut(s) | 1542 |
| BsrDI | GCAATG | 1 cut(s) | 1289 |
| BsrGI | TGTACA | 1 cut(s) | 732 |
| BsrI | ACTGG | 4 cut(s) | 638, 823, 1262, 1465 |
| BssECI | CCNNGG | 5 cut(s) | 271, 484, 574, 588, 1563 |
| BssMI | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| BssNAI | GTATAC | 1 cut(s) | 1458 |
| BssNI | GRCGYC | 2 cut(s) | 421, 1275 |
| BssT1I | CCWWGG | 2 cut(s) | 271, 588 |
| Bst1107I | GTATAC | 1 cut(s) | 1458 |
| Bst2UI | CCWGG | 4 cut(s) | 437, 1058, 1271, 1564 |
| Bst4CI | ACNGT | 4 cut(s) | 499, 575, 835, 1345 |
| Bst6I | CTCTTC | 2 cut(s) | 1343, 1506 |
| BstACI | GRCGYC | 2 cut(s) | 421, 1275 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 911 |
| BstAUI | TGTACA | 1 cut(s) | 732 |
| BstBI | TTCGAA | 1 cut(s) | 1080 |
| BstC8I | GCNNGC | 5 cut(s) | 412, 568, 903, 999, 1415 |
| BstDEI | CTNAG | 4 cut(s) | 361, 930, 1134, 1200 |
| BstDSI | CCRYGG | 2 cut(s) | 271, 574 |
| BstF5I | GGATG | 3 cut(s) | 705, 1173, 1198 |
| BstFNI | CGCG | 3 cut(s) | 408, 717, 1377 |
| BstHHI | GCGC | 4 cut(s) | 410, 898, 1379, 1426 |
| BstKTI | GATC | 4 cut(s) | 175, 645, 1360, 1574 |
| BstMAI | GTCTC | 2 cut(s) | 741, 1542 |
| BstMBI | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| BstNI | CCWGG | 4 cut(s) | 437, 1058, 1271, 1564 |
| BstNSI | RCATGY | 2 cut(s) | 1001, 1118 |
| BstSCI | CCNGG | 6 cut(s) | 435, 483, 484, 1056, 1269, 1562 |
| BstUI | CGCG | 3 cut(s) | 408, 717, 1377 |
| BstV1I | GCAGC | 9 cut(s) | 222, 225, 228, 231, 382, 910, 926, 1016, 1023 |
| BstV2I | GAAGAC | 1 cut(s) | 246 |
| BstX2I | RGATCY | 1 cut(s) | 1357 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 272, 410, 595 |
| BstYI | RGATCY | 1 cut(s) | 1357 |
| BstZ17I | GTATAC | 1 cut(s) | 1458 |
| BsuRI | GGCC | 8 cut(s) | 414, 455, 477, 489, 522, 1232, 1476, 1562 |
| BtgI | CCRYGG | 2 cut(s) | 271, 574 |
| BtgZI | GCGATG | 1 cut(s) | 698 |
| BtrI | CACGTC | 1 cut(s) | 1021 |
| BtsCI | GGATG | 3 cut(s) | 705, 1173, 1198 |
| BtsI | GCAGTG | 3 cut(s) | 819, 945, 1125 |
| BtsIMutI | CAGTG | 4 cut(s) | 819, 945, 1125, 1269 |
| BveI | ACCTGC | 1 cut(s) | 476 |
| Cac8I | GCNNGC | 5 cut(s) | 412, 568, 903, 999, 1415 |
| CaiI | CAGNNNCTG | 1 cut(s) | 818 |
| CciI | TCATGA | 1 cut(s) | 781 |
| CfoI | GCGC | 4 cut(s) | 410, 898, 1379, 1426 |
| Cfr13I | GGNCC | 7 cut(s) | 10, 413, 487, 529, 1230, 1475, 1560 |
| Cfr9I | CCCGGG | 1 cut(s) | 484 |
| CseI | GACGC | 1 cut(s) | 429 |
| Csp6I | GTAC | 4 cut(s) | 733, 978, 1351, 1408 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 628, 663 |
| CviAII | CATG | 9 cut(s) | 272, 782, 920, 998, 1115, 1174, 1369, 1439, 1447 |
| CviQI | GTAC | 4 cut(s) | 733, 978, 1351, 1408 |
| DdeI | CTNAG | 4 cut(s) | 361, 930, 1134, 1200 |
| DpnI | GATC | 4 cut(s) | 174, 644, 1359, 1573 |
| DpnII | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| Eam1104I | CTCTTC | 2 cut(s) | 1343, 1506 |
| EarI | CTCTTC | 2 cut(s) | 1343, 1506 |
| EciI | GGCGGA | 2 cut(s) | 23, 296 |
| Ecl136II | GAGCTC | 2 cut(s) | 511, 1336 |
| Eco130I | CCWWGG | 2 cut(s) | 271, 588 |
| Eco24I | GRGCYC | 2 cut(s) | 513, 1338 |
| Eco31I | GGTCTC | 1 cut(s) | 1542 |
| Eco47I | GGWCC | 2 cut(s) | 10, 529 |
| Eco53kI | GAGCTC | 2 cut(s) | 511, 1336 |
| Eco88I | CYCGRG | 1 cut(s) | 484 |
| EcoICRI | GAGCTC | 2 cut(s) | 511, 1336 |
| EcoO109I | RGGNCCY | 1 cut(s) | 529 |
| EcoRI | GAATTC | 1 cut(s) | 1082 |
| EcoRII | CCWGG | 4 cut(s) | 435, 1056, 1269, 1562 |
| EcoT14I | CCWWGG | 2 cut(s) | 271, 588 |
| EcoT38I | GRGCYC | 2 cut(s) | 513, 1338 |
| ErhI | CCWWGG | 2 cut(s) | 271, 588 |
| Esp3I | CGTCTC | 1 cut(s) | 741 |
| FaeI | CATG | 9 cut(s) | 275, 785, 923, 1001, 1118, 1177, 1372, 1442, 1450 |
| FaqI | GGGAC | 2 cut(s) | 542, 1367 |
| FatI | CATG | 9 cut(s) | 271, 781, 919, 997, 1114, 1173, 1368, 1438, 1446 |
| FblI | GTMKAC | 2 cut(s) | 318, 1457 |
| FokI | GGATG | 3 cut(s) | 692, 1180, 1205 |
| FriOI | GRGCYC | 2 cut(s) | 513, 1338 |
| FspBI | CTAG | 4 cut(s) | 159, 339, 1545, 1609 |
| FspI | TGCGCA | 1 cut(s) | 1425 |
| GlaI | GCGC | 4 cut(s) | 409, 897, 1378, 1425 |
| GsuI | CTGGAG | 2 cut(s) | 644, 652 |
| HaeIII | GGCC | 8 cut(s) | 414, 455, 477, 489, 522, 1232, 1476, 1562 |
| HapII | CCGG | 2 cut(s) | 485, 1143 |
| HgaI | GACGC | 1 cut(s) | 429 |
| HhaI | GCGC | 4 cut(s) | 410, 898, 1379, 1426 |
| Hin1I | GRCGYC | 2 cut(s) | 421, 1275 |
| Hin1II | CATG | 9 cut(s) | 275, 785, 923, 1001, 1118, 1177, 1372, 1442, 1450 |
| Hin6I | GCGC | 4 cut(s) | 408, 896, 1377, 1424 |
| HinP1I | GCGC | 4 cut(s) | 408, 896, 1377, 1424 |
| HindIII | AAGCTT | 1 cut(s) | 620 |
| HinfI | GANTC | 5 cut(s) | 787, 823, 873, 1250, 1443 |
| HpaII | CCGG | 2 cut(s) | 485, 1143 |
| HphI | GGTGA | 3 cut(s) | 457, 970, 1570 |
| Hpy166II | GTNNAC | 5 cut(s) | 319, 628, 838, 1024, 1458 |
| Hpy188I | TCNGA | 8 cut(s) | 295, 795, 828, 872, 1203, 1297, 1571, 1599 |
| Hpy188III | TCNNGA | 6 cut(s) | 506, 661, 669, 782, 877, 1040 |
| Hpy8I | GTNNAC | 5 cut(s) | 319, 628, 838, 1024, 1458 |
| Hpy99I | CGWCG | 5 cut(s) | 299, 423, 503, 795, 1280 |
| HpyAV | CCTTC | 2 cut(s) | 259, 1055 |
| HpyCH4III | ACNGT | 4 cut(s) | 499, 575, 835, 1345 |
| HpyCH4IV | ACGT | 3 cut(s) | 297, 1020, 1275 |
| HpyF3I | CTNAG | 4 cut(s) | 361, 930, 1134, 1200 |
| HpySE526I | ACGT | 3 cut(s) | 297, 1020, 1275 |
| Hsp92I | GRCGYC | 2 cut(s) | 421, 1275 |
| Hsp92II | CATG | 9 cut(s) | 275, 785, 923, 1001, 1118, 1177, 1372, 1442, 1450 |
| HspAI | GCGC | 4 cut(s) | 408, 896, 1377, 1424 |
| Kzo9I | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| LguI | GCTCTTC | 1 cut(s) | 1343 |
| LmnI | GCTCC | 6 cut(s) | 55, 117, 207, 1139, 1297, 1321 |
| Lsp1109I | GCAGC | 9 cut(s) | 222, 225, 228, 231, 382, 910, 926, 1016, 1023 |
| LweI | GCATC | 4 cut(s) | 376, 691, 747, 1186 |
| MaeI | CTAG | 4 cut(s) | 159, 339, 1545, 1609 |
| MaeII | ACGT | 3 cut(s) | 297, 1020, 1275 |
| MaeIII | GTNAC | 4 cut(s) | 463, 814, 1016, 1576 |
| MalI | GATC | 4 cut(s) | 174, 644, 1359, 1573 |
| MboI | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| MboII | GAAGA | 4 cut(s) | 251, 677, 1330, 1523 |
| MflI | RGATCY | 1 cut(s) | 1357 |
| MhlI | GDGCHC | 3 cut(s) | 513, 1326, 1338 |
| MluCI | AATT | 6 cut(s) | 81, 549, 593, 1035, 1044, 1082 |
| MlyI | GAGTC | 1 cut(s) | 1452 |
| MmeI | TCCRAC | 4 cut(s) | 318, 733, 773, 1275 |
| MseI | TTAA | 3 cut(s) | 80, 612, 965 |
| MslI | CAYNNNNRTG | 3 cut(s) | 270, 1329, 1367 |
| MspA1I | CMGCKG | 2 cut(s) | 730, 1235 |
| MspI | CCGG | 2 cut(s) | 485, 1143 |
| MspR9I | CCNGG | 6 cut(s) | 437, 485, 486, 1058, 1271, 1564 |
| Mva1269I | GAATGC | 1 cut(s) | 1426 |
| MvaI | CCWGG | 4 cut(s) | 437, 1058, 1271, 1564 |
| MvnI | CGCG | 3 cut(s) | 408, 717, 1377 |
| NciI | CCSGG | 2 cut(s) | 485, 486 |
| NcoI | CCATGG | 1 cut(s) | 271 |
| NdeII | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| NlaIII | CATG | 9 cut(s) | 275, 785, 923, 1001, 1118, 1177, 1372, 1442, 1450 |
| NlaIV | GGNNCC | 8 cut(s) | 12, 57, 530, 600, 652, 1141, 1259, 1477 |
| NmuCI | GTSAC | 4 cut(s) | 463, 814, 1016, 1576 |
| NsbI | TGCGCA | 1 cut(s) | 1425 |
| NspI | RCATGY | 2 cut(s) | 1001, 1118 |
| NspV | TTCGAA | 1 cut(s) | 1080 |
| PaeI | GCATGC | 1 cut(s) | 1001 |
| PagI | TCATGA | 1 cut(s) | 781 |
| PaqCI | CACCTGC | 1 cut(s) | 476 |
| PciSI | GCTCTTC | 1 cut(s) | 1343 |
| PctI | GAATGC | 1 cut(s) | 1426 |
| PfeI | GAWTC | 4 cut(s) | 787, 823, 873, 1250 |
| PflFI | GACNNNGTC | 1 cut(s) | 299 |
| PfoI | TCCNGGA | 1 cut(s) | 1269 |
| PleI | GAGTC | 1 cut(s) | 1451 |
| PpsI | GAGTC | 1 cut(s) | 1451 |
| PpuMI | RGGWCCY | 1 cut(s) | 529 |
| Psp124BI | GAGCTC | 2 cut(s) | 513, 1338 |
| Psp5II | RGGWCCY | 1 cut(s) | 529 |
| Psp6I | CCWGG | 4 cut(s) | 435, 1056, 1269, 1562 |
| PspGI | CCWGG | 4 cut(s) | 435, 1056, 1269, 1562 |
| PspN4I | GGNNCC | 8 cut(s) | 12, 57, 530, 600, 652, 1141, 1259, 1477 |
| PspPI | GGNCC | 7 cut(s) | 10, 413, 487, 529, 1230, 1475, 1560 |
| PspPPI | RGGWCCY | 1 cut(s) | 529 |
| PstNI | CAGNNNCTG | 1 cut(s) | 818 |
| PsuI | RGATCY | 1 cut(s) | 1357 |
| PsyI | GACNNNGTC | 1 cut(s) | 299 |
| PvuII | CAGCTG | 1 cut(s) | 730 |
| RsaI | GTAC | 4 cut(s) | 734, 979, 1352, 1409 |
| RsaNI | GTAC | 4 cut(s) | 733, 978, 1351, 1408 |
| RseI | CAYNNNNRTG | 3 cut(s) | 270, 1329, 1367 |
| SacI | GAGCTC | 2 cut(s) | 513, 1338 |
| SapI | GCTCTTC | 1 cut(s) | 1343 |
| SaqAI | TTAA | 3 cut(s) | 80, 612, 965 |
| Sau3AI | GATC | 4 cut(s) | 172, 642, 1357, 1571 |
| Sau96I | GGNCC | 7 cut(s) | 10, 413, 487, 529, 1230, 1475, 1560 |
| SchI | GAGTC | 1 cut(s) | 1452 |
| ScrFI | CCNGG | 6 cut(s) | 437, 485, 486, 1058, 1271, 1564 |
| SduI | GDGCHC | 3 cut(s) | 513, 1326, 1338 |
| SfaNI | GCATC | 4 cut(s) | 376, 691, 747, 1186 |
| SfuI | TTCGAA | 1 cut(s) | 1080 |
| SinI | GGWCC | 2 cut(s) | 10, 529 |
| SmaI | CCCGGG | 1 cut(s) | 486 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 270, 1329, 1367 |
| SmlI | CTYRAG | 2 cut(s) | 1088, 1289 |
| SmoI | CTYRAG | 2 cut(s) | 1088, 1289 |
| SpeI | ACTAGT | 1 cut(s) | 158 |
| SphI | GCATGC | 1 cut(s) | 1001 |
| SrfI | GCCCGGGC | 1 cut(s) | 486 |
| Sse9I | AATT | 6 cut(s) | 81, 549, 593, 1035, 1044, 1082 |
| SsiI | CCGC | 9 cut(s) | 8, 195, 307, 406, 456, 471, 717, 767, 1233 |
| SspI | AATATT | 1 cut(s) | 808 |
| SspMI | CTAG | 4 cut(s) | 159, 339, 1545, 1609 |
| SstI | GAGCTC | 2 cut(s) | 513, 1338 |
| StyD4I | CCNGG | 6 cut(s) | 435, 483, 484, 1056, 1269, 1562 |
| StyI | CCWWGG | 2 cut(s) | 271, 588 |
| TaaI | ACNGT | 4 cut(s) | 499, 575, 835, 1345 |
| TaiI | ACGT | 3 cut(s) | 300, 1023, 1278 |
| TaqI | TCGA | 5 cut(s) | 505, 699, 973, 1080, 1539 |
| TasI | AATT | 6 cut(s) | 81, 549, 593, 1035, 1044, 1082 |
| TatI | WGTACW | 4 cut(s) | 732, 977, 1350, 1407 |
| TauI | GCSGC | 4 cut(s) | 198, 458, 720, 1235 |
| TfiI | GAWTC | 4 cut(s) | 787, 823, 873, 1250 |
| Tru1I | TTAA | 3 cut(s) | 80, 612, 965 |
| Tru9I | TTAA | 3 cut(s) | 80, 612, 965 |
| TscAI | CASTG | 4 cut(s) | 819, 945, 1125, 1269 |
| TseFI | GTSAC | 4 cut(s) | 463, 814, 1016, 1576 |
| TseI | GCWGC | 9 cut(s) | 210, 213, 216, 219, 395, 898, 914, 1011, 1029 |
| Tsp45I | GTSAC | 4 cut(s) | 463, 814, 1016, 1576 |
| TspDTI | ATGAA | 4 cut(s) | 770, 1263, 1427, 1548 |
| TspGWI | ACGGA | 2 cut(s) | 68, 533 |
| TspMI | CCCGGG | 1 cut(s) | 484 |
| TspRI | CASTG | 4 cut(s) | 819, 945, 1125, 1269 |
| Tth111I | GACNNNGTC | 1 cut(s) | 299 |
| VpaK11BI | GGWCC | 2 cut(s) | 10, 529 |
| XapI | RAATTY | 3 cut(s) | 549, 1044, 1082 |
| XceI | RCATGY | 2 cut(s) | 1001, 1118 |
| XmaI | CCCGGG | 1 cut(s) | 484 |
| XmiI | GTMKAC | 2 cut(s) | 318, 1457 |
| XspI | CTAG | 4 cut(s) | 159, 339, 1545, 1609 |
| ZraI | GACGTC | 1 cut(s) | 1276 |