RLG00000030852

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014539
Physical Location & Seq
Forward (+)
208211 .. 210047
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030852

Sequence Viewer

Length: 1383 bp
ATGGCAGAACCCACAACAGAGGAGATGATCTTGTCATTAGCTCCGGTGAAGCTCCACAAACTACCCCACCTGACCGACTATCTCTCACAACTCCCCACCCACACCAACCCACTCCAACACAACCCCTTCTACCACCCAACCGACGGCTTCTACATAACCCCATCCGATGTCATCCTCCGCCAGATTGTCTACGACCTAGCCGTCTCCGTCGGCTCCTCCGCTCATCCCGCCTCAGCTTACCATCGCGCCGGCCCACGACGGCACATCTTGTTTGAGCCGTCGAGCATCAGGGCGGCTATTGTGACATGCGGTGGGCTGTGCCCGGGCATGAACACGGTGGTGAGAGAGCTGGTTTTTGGCCTCTGGGACCTCTACGGAGTCCGTCAGATTTTTGGTATCAAGGCTGGCTATCGTGGATTTTACTCTATGGATCCCATCCAGCTTAACCCAAAGCTCGTCGACCACTGGCACAAGATTGGCGGCACTGTCCTCGAGACCTCCAGAGGTGGTTTTCATCTTCAAAAGATTGTCGATGCCATCCAACATCGCGGCTATAATCAGGTGTACATTATAGGTGGAGATGGCACAATGCGGGGGGCAGTTGAGATATTTCATGAGATTTGTAGGCGAAAACTGAACATTGCGGTTACTGGGATTCCAAAAACAGTTGACAATGATGTTGGCATCATTGACAGATCTTTCGGATTCCAGACAGCAGTAGAAATGGCCCAGCAGGCAATCAGTGCAGCTCATGTGGAGGCTGAAAGTGCAGTGAATGGAATAGGCTTGGTCAAGTTGATGGGTCGAAGTACAGGCCACATTGCTCTCTATGCAACATTGAGCAGTCGAGACGTGGACTGCTGCCTAATCCCAGAAACTGAGTTTTACCTGGAAGGAAAAGGAGGGCTATTTGAATTCCTTGAGCAGCGACTGAGGGAGAATGGACACGCAGTGTTAGTTGTGGCTGAAGGAGCGGGACAGGACATAATTCCAAGGAACGAGGCTCAGAAACAGGAGAGGGATGAATCTGGAAACCCAGTGTTCCTAGATGTTGGCCAATGGTTGAAGTCGGAGCTCAATAATTGGTGGAGCAGGGATCACCCCGATGAGCTCTTCACAGTCAAGTACATAGATCCTACATACATGATACGGGCCGTCCGTGCAAATGCTACGGATAACTTGTACTGTACACTTCTTGCCCATTCAGCTATTCATGGAGTGATGGCTGGTTACACCGGATTCGTCCCTGGCCCTATCAATGGCAATTATGCTTACATTCCAGTTGAAGAGGTAGCAAAGGCTAAGAATGAGGTCAACACGAGAGACCACAAATGGGCGTGGGTAAGATCTGTCACAAACCAGCCTGATTTTATGAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

461

Amino Acids

51.2

Weight (kDa)

6.28

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 97 - 405 1.6e-54 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AccBSI CCGCTC 2 cut(s) 221, 974
AccI GTMKAC 2 cut(s) 189, 459
AccII CGCG 2 cut(s) 246, 549
AclWI GGATC 4 cut(s) 425, 438, 1104, 1127
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 1 cut(s) 987
AdeI CACNNNGTG 1 cut(s) 952
AfaI GTAC 5 cut(s) 566, 811, 1127, 1184, 1189
AfiI CCNNNNNNNGG 3 cut(s) 143, 1259, 1333
AgsI TTSAA 4 cut(s) 521, 914, 1066, 1286
AjiI CACGTC 1 cut(s) 853
AjnI CCWGG 2 cut(s) 888, 1246
AleI CACNNNNGTG 1 cut(s) 338
Alw21I GWGCWC 2 cut(s) 1077, 1113
Alw26I GTCTC 4 cut(s) 208, 488, 843, 1317
AlwI GGATC 4 cut(s) 425, 438, 1104, 1127
AlwNI CAGNNNCTG 2 cut(s) 878, 931
Ama87I CYCGRG 2 cut(s) 322, 491
AoxI GGCC 7 cut(s) 250, 358, 726, 814, 1054, 1152, 1249
ApeKI GCWGC 3 cut(s) 746, 861, 925
ApoI RAATTY 1 cut(s) 914
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 5 cut(s) 251, 367, 727, 1152, 1250
AsuC2I CCSGG 2 cut(s) 323, 324
AsuHPI GGTGA 3 cut(s) 58, 352, 1091
AvaI CYCGRG 2 cut(s) 322, 491
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 1056
BamHI GGATCC 1 cut(s) 430
BanII GRGCYC 2 cut(s) 1077, 1113
BauI CACGAG 1 cut(s) 1318
Bbv12I GWGCWC 2 cut(s) 1077, 1113
BbvCI CCTCAGC 1 cut(s) 232
BbvI GCAGC 3 cut(s) 758, 848, 937
BccI CCATC 7 cut(s) 169, 249, 443, 545, 575, 793, 1216
BceAI ACGGC 5 cut(s) 160, 185, 262, 275, 1139
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BciT130I CCWGG 2 cut(s) 890, 1248
BcnI CCSGG 2 cut(s) 323, 324
BcoDI GTCTC 4 cut(s) 208, 488, 843, 1317
BfaI CTAG 2 cut(s) 197, 1046
BglI GCCNNNNNGGC 1 cut(s) 734
BglII AGATCT 2 cut(s) 695, 1346
BisI GCNGC 6 cut(s) 294, 481, 550, 747, 862, 926
BlsI GCNGC 6 cut(s) 295, 482, 551, 748, 863, 927
Bme1390I CCNGG 4 cut(s) 323, 324, 890, 1248
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 2 cut(s) 322, 491
BmgBI CACGTC 1 cut(s) 853
BmgT120I GGNCC 5 cut(s) 251, 367, 727, 1152, 1250
BmiI GGNNCC 3 cut(s) 214, 368, 432
BmrFI CCNGG 4 cut(s) 323, 324, 890, 1248
BmrI ACTGGG 2 cut(s) 660, 1031
BmsI GCATC 3 cut(s) 294, 523, 693
BmuI ACTGGG 2 cut(s) 660, 1031
BpmI CTGGAG 1 cut(s) 484
Bpu10I CCTNAGC 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 2 cut(s) 323, 324
BsaI GGTCTC 2 cut(s) 488, 1317
BsaJI CCNNGG 3 cut(s) 322, 992, 1246
BsaWI WCCGGW 2 cut(s) 43, 1235
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 1259, 1333
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 4 cut(s) 470, 655, 1037, 1280
Bse3DI GCAATG 2 cut(s) 639, 819
BseBI CCWGG 2 cut(s) 890, 1248
BseDI CCNNGG 3 cut(s) 322, 992, 1246
BseGI GGATG 6 cut(s) 161, 171, 223, 435, 537, 1027
BseLI CCNNNNNNNGG 3 cut(s) 143, 1259, 1333
BseMI GCAATG 2 cut(s) 639, 819
BseMII CTCAG 4 cut(s) 246, 870, 923, 1019
BseNI ACTGG 4 cut(s) 470, 655, 1037, 1280
BseRI GAGGAG 2 cut(s) 35, 205
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 3 cut(s) 758, 848, 937
BseYI CCCAGC 1 cut(s) 729
BsgI GTGCAG 2 cut(s) 765, 789
Bsh1236I CGCG 2 cut(s) 246, 549
BshFI GGCC 7 cut(s) 252, 360, 728, 816, 1056, 1154, 1251
BsiHKAI GWGCWC 2 cut(s) 1077, 1113
BsiHKCI CYCGRG 2 cut(s) 322, 491
BsiSI CCGG 4 cut(s) 44, 249, 323, 1236
BslFI GGGAC 3 cut(s) 380, 990, 1229
BslI CCNNNNNNNGG 3 cut(s) 143, 1259, 1333
BsmAI GTCTC 4 cut(s) 208, 488, 843, 1317
BsmBI CGTCTC 2 cut(s) 208, 843
BsmFI GGGAC 3 cut(s) 380, 990, 1229
BsnI GGCC 7 cut(s) 252, 360, 728, 816, 1056, 1154, 1251
Bso31I GGTCTC 2 cut(s) 488, 1317
BsoBI CYCGRG 2 cut(s) 322, 491
Bsp1286I GDGCHC 3 cut(s) 323, 1077, 1113
Bsp1407I TGTACA 2 cut(s) 564, 1187
Bsp143I GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
BspANI GGCC 7 cut(s) 252, 360, 728, 816, 1056, 1154, 1251
BspCNI CTCAG 4 cut(s) 245, 871, 924, 1018
BspFNI CGCG 2 cut(s) 246, 549
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 3 cut(s) 214, 368, 432
BspPI GGATC 4 cut(s) 425, 438, 1104, 1127
BspQI GCTCTTC 1 cut(s) 1118
BspTNI GGTCTC 2 cut(s) 488, 1317
BsrBI CCGCTC 2 cut(s) 221, 974
BsrDI GCAATG 2 cut(s) 639, 819
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 2 cut(s) 564, 1187
BsrI ACTGG 4 cut(s) 470, 655, 1037, 1280
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 322, 992, 1246
BssMI GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
BssSI CACGAG 1 cut(s) 1318
BssT1I CCWWGG 1 cut(s) 992
Bst2BI CACGAG 1 cut(s) 1318
Bst2UI CCWGG 2 cut(s) 890, 1248
Bst4CI ACNGT 5 cut(s) 337, 487, 667, 1120, 1187
Bst6I CTCTTC 2 cut(s) 1118, 1281
BstAPI GCANNNNNTGC 1 cut(s) 743
BstAUI TGTACA 2 cut(s) 564, 1187
BstC8I GCNNGC 3 cut(s) 250, 406, 735
BstDEI CTNAG 5 cut(s) 232, 879, 932, 1005, 1302
BstF5I GGATG 6 cut(s) 161, 171, 223, 435, 537, 1027
BstFNI CGCG 2 cut(s) 246, 549
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 6 cut(s) 30, 433, 698, 1099, 1135, 1349
BstMAI GTCTC 4 cut(s) 208, 488, 843, 1317
BstMBI GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
BstMWI GCNNNNNNNGC 8 cut(s) 227, 734, 743, 767, 830, 971, 1160, 1205
BstNI CCWGG 2 cut(s) 890, 1248
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 4 cut(s) 321, 322, 888, 1246
BstSLI GKGCMC 1 cut(s) 323
BstUI CGCG 2 cut(s) 246, 549
BstV1I GCAGC 3 cut(s) 758, 848, 937
BstX2I RGATCY 4 cut(s) 430, 695, 1132, 1346
BstYI RGATCY 4 cut(s) 430, 695, 1132, 1346
BsuRI GGCC 7 cut(s) 252, 360, 728, 816, 1056, 1154, 1251
BtgZI GCGATG 2 cut(s) 227, 530
BtrI CACGTC 1 cut(s) 853
BtsCI GGATG 6 cut(s) 161, 171, 223, 435, 537, 1027
BtsI GCAGTG 2 cut(s) 777, 957
BtsIMutI CAGTG 6 cut(s) 463, 483, 748, 777, 957, 1044
Cac8I GCNNGC 3 cut(s) 250, 406, 735
CaiI CAGNNNCTG 2 cut(s) 878, 931
CciI TCATGA 1 cut(s) 613
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 5 cut(s) 251, 367, 727, 1152, 1250
Cfr9I CCCGGG 1 cut(s) 322
Csp6I GTAC 5 cut(s) 565, 810, 1126, 1183, 1188
CviAII CATG 6 cut(s) 306, 328, 614, 752, 1144, 1214
CviQI GTAC 5 cut(s) 565, 810, 1126, 1183, 1188
DdeI CTNAG 5 cut(s) 232, 879, 932, 1005, 1302
DpnI GATC 6 cut(s) 29, 432, 697, 1098, 1134, 1348
DpnII GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
DraIII CACNNNGTG 1 cut(s) 952
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EaeI YGGCCR 1 cut(s) 1054
Eam1104I CTCTTC 2 cut(s) 1118, 1281
EarI CTCTTC 2 cut(s) 1118, 1281
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 1075, 1111
Eco130I CCWWGG 1 cut(s) 992
Eco24I GRGCYC 2 cut(s) 1077, 1113
Eco31I GGTCTC 2 cut(s) 488, 1317
Eco47I GGWCC 1 cut(s) 367
Eco53kI GAGCTC 2 cut(s) 1075, 1111
Eco57I CTGAAG 1 cut(s) 987
Eco88I CYCGRG 2 cut(s) 322, 491
EcoICRI GAGCTC 2 cut(s) 1075, 1111
EcoO109I RGGNCCY 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 2 cut(s) 888, 1246
EcoT14I CCWWGG 1 cut(s) 992
EcoT38I GRGCYC 2 cut(s) 1077, 1113
ErhI CCWWGG 1 cut(s) 992
Esp3I CGTCTC 2 cut(s) 208, 843
FaeI CATG 6 cut(s) 309, 331, 617, 755, 1147, 1217
FaqI GGGAC 3 cut(s) 380, 990, 1229
FatI CATG 6 cut(s) 305, 327, 613, 751, 1143, 1213
FauI CCCGC 3 cut(s) 235, 585, 967
FblI GTMKAC 2 cut(s) 189, 459
Fnu4HI GCNGC 6 cut(s) 294, 481, 550, 747, 862, 926
FokI GGATG 6 cut(s) 148, 158, 210, 422, 524, 1034
FriOI GRGCYC 2 cut(s) 1077, 1113
Fsp4HI GCNGC 6 cut(s) 294, 481, 550, 747, 862, 926
FspBI CTAG 2 cut(s) 197, 1046
GlaI GCGC 1 cut(s) 247
GluI GCNGC 6 cut(s) 294, 481, 550, 747, 862, 926
GsaI CCCAGC 1 cut(s) 733
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 7 cut(s) 252, 360, 728, 816, 1056, 1154, 1251
HapII CCGG 4 cut(s) 44, 249, 323, 1236
HhaI GCGC 1 cut(s) 248
Hin1II CATG 6 cut(s) 309, 331, 617, 755, 1147, 1217
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 3 cut(s) 460, 670, 1315
HindII GTYRAC 3 cut(s) 460, 670, 1315
HinfI GANTC 5 cut(s) 378, 655, 705, 1025, 1239
HpaII CCGG 4 cut(s) 44, 249, 323, 1236
HphI GGTGA 3 cut(s) 58, 352, 1091
Hpy166II GTNNAC 7 cut(s) 190, 460, 565, 670, 856, 1190, 1315
Hpy188I TCNGA 5 cut(s) 166, 387, 704, 1008, 1072
Hpy188III TCNNGA 6 cut(s) 493, 501, 614, 709, 848, 1029
Hpy8I GTNNAC 7 cut(s) 190, 460, 565, 670, 856, 1190, 1315
Hpy99I CGWCG 5 cut(s) 146, 212, 261, 283, 461
HpyAV CCTTC 3 cut(s) 136, 887, 962
HpyCH4III ACNGT 5 cut(s) 337, 487, 667, 1120, 1187
HpyCH4IV ACGT 1 cut(s) 852
HpyCH4V TGCA 4 cut(s) 746, 770, 833, 1163
HpyF10VI GCNNNNNNNGC 8 cut(s) 227, 734, 743, 767, 830, 971, 1160, 1205
HpyF3I CTNAG 5 cut(s) 232, 879, 932, 1005, 1302
HpySE526I ACGT 1 cut(s) 852
Hsp92II CATG 6 cut(s) 309, 331, 617, 755, 1147, 1217
HspAI GCGC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
LguI GCTCTTC 1 cut(s) 1118
LmnI GCTCC 6 cut(s) 46, 57, 218, 971, 1072, 1089
Lsp1109I GCAGC 3 cut(s) 758, 848, 937
LweI GCATC 3 cut(s) 294, 523, 693
MaeI CTAG 2 cut(s) 197, 1046
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 4 cut(s) 301, 646, 1229, 1351
MalI GATC 6 cut(s) 29, 432, 697, 1098, 1134, 1348
MbiI CCGCTC 2 cut(s) 221, 974
MboI GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
MboII GAAGA 3 cut(s) 509, 1105, 1298
MflI RGATCY 4 cut(s) 430, 695, 1132, 1346
MhlI GDGCHC 3 cut(s) 323, 1077, 1113
MlsI TGGCCA 1 cut(s) 1056
MluCI AATT 4 cut(s) 914, 987, 1081, 1264
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 1 cut(s) 387
MmeI TCCRAC 3 cut(s) 139, 565, 1050
Mox20I TGGCCA 1 cut(s) 1056
MroNI GCCGGC 1 cut(s) 248
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 2 cut(s) 338, 1104
Msp20I TGGCCA 1 cut(s) 1056
MspI CCGG 4 cut(s) 44, 249, 323, 1236
MspR9I CCNGG 4 cut(s) 323, 324, 890, 1248
MvaI CCWGG 2 cut(s) 890, 1248
MvnI CGCG 2 cut(s) 246, 549
MwoI GCNNNNNNNGC 8 cut(s) 227, 734, 743, 767, 830, 971, 1160, 1205
NaeI GCCGGC 1 cut(s) 250
NciI CCSGG 2 cut(s) 323, 324
NdeII GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 6 cut(s) 309, 331, 617, 755, 1147, 1217
NlaIV GGNNCC 3 cut(s) 214, 368, 432
NmuCI GTSAC 2 cut(s) 301, 1351
NspI RCATGY 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 491
PagI TCATGA 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 1118
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 1156
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 4 cut(s) 655, 705, 1025, 1239
PkrI GCNGC 6 cut(s) 295, 482, 551, 748, 863, 927
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
PpuMI RGGWCCY 1 cut(s) 367
Psp124BI GAGCTC 2 cut(s) 1077, 1113
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 888, 1246
PspFI CCCAGC 1 cut(s) 729
PspGI CCWGG 2 cut(s) 888, 1246
PspN4I GGNNCC 3 cut(s) 214, 368, 432
PspPI GGNCC 5 cut(s) 251, 367, 727, 1152, 1250
PspPPI RGGWCCY 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 878, 931
PsuI RGATCY 4 cut(s) 430, 695, 1132, 1346
RsaI GTAC 5 cut(s) 566, 811, 1127, 1184, 1189
RsaNI GTAC 5 cut(s) 565, 810, 1126, 1183, 1188
RseI CAYNNNNRTG 2 cut(s) 338, 1104
SacI GAGCTC 2 cut(s) 1077, 1113
SalI GTCGAC 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 1118
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 6 cut(s) 294, 481, 550, 747, 862, 926
Sau3AI GATC 6 cut(s) 27, 430, 695, 1096, 1132, 1346
Sau96I GGNCC 5 cut(s) 251, 367, 727, 1152, 1250
SchI GAGTC 1 cut(s) 387
ScrFI CCNGG 4 cut(s) 323, 324, 890, 1248
SduI GDGCHC 3 cut(s) 323, 1077, 1113
SfaNI GCATC 3 cut(s) 294, 523, 693
Sfr274I CTCGAG 1 cut(s) 491
SinI GGWCC 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 491
SmaI CCCGGG 1 cut(s) 324
SmiMI CAYNNNNRTG 2 cut(s) 338, 1104
SmlI CTYRAG 2 cut(s) 491, 920
SmoI CTYRAG 2 cut(s) 491, 920
SrfI GCCCGGGC 1 cut(s) 324
Sse9I AATT 4 cut(s) 914, 987, 1081, 1264
SspMI CTAG 2 cut(s) 197, 1046
SstI GAGCTC 2 cut(s) 1077, 1113
StyD4I CCNGG 4 cut(s) 321, 322, 888, 1246
StyI CCWWGG 1 cut(s) 992
TaaI ACNGT 5 cut(s) 337, 487, 667, 1120, 1187
TaiI ACGT 1 cut(s) 855
TaqI TCGA 6 cut(s) 281, 459, 492, 531, 805, 847
TaqII GACCGA 1 cut(s) 89
TasI AATT 4 cut(s) 914, 987, 1081, 1264
TatI WGTACW 5 cut(s) 564, 809, 1125, 1182, 1187
TauI GCSGC 3 cut(s) 296, 483, 552
TfiI GAWTC 4 cut(s) 655, 705, 1025, 1239
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 6 cut(s) 470, 490, 748, 777, 957, 1044
TseFI GTSAC 2 cut(s) 301, 1351
TseI GCWGC 3 cut(s) 746, 861, 925
Tsp45I GTSAC 2 cut(s) 301, 1351
TspDTI ATGAA 5 cut(s) 344, 503, 602, 1038, 1202
TspGWI ACGGA 5 cut(s) 196, 371, 390, 1148, 1187
TspMI CCCGGG 1 cut(s) 322
TspRI CASTG 6 cut(s) 470, 490, 748, 777, 957, 1044
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 309
XhoI CTCGAG 1 cut(s) 491
XmaI CCCGGG 1 cut(s) 322
XmiI GTMKAC 2 cut(s) 189, 459
XspI CTAG 2 cut(s) 197, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.