MD05G1363600.v1.1

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014539
Physical Location & Seq
Reverse (-)
47837762 .. 47840607
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1363600.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGGCCGTGGCGCAGACAGACCCAGCAGCAGCAGCAGCAACGAGAAGACATGATGATGATGATGAAGATCGGGTGATGATATACTTGCCCTTCTCTAATCGTGTGAAGCTCCAGAAACTACCCCTCCTAACTGATCATCTCTCGCTCGGATCAGATTTAGACCTAGTTCAAAGTAACCTCATCAACACCAACCCACTAGAACACAACCCCTTCTTCTCCCAGTCCGATTGTTTCTACATAGCCCCATCTGATGTCGTCCTCCGCCACATCATCTACGACTACAAACACCCTCAACCGAAAGCTGCAGCAGCAGGTGGAGGTGGCGGTGAAGAAGAAAAACAAGGCTACTCTAGTACTACTACTAGTGCCTTGGCATACCACCGCGCCGGCCCACGACGCCAAATCTTGTTCGATGCGTCCAGGATCAAGGCGGCTATTGTGACCTGCGGTGGCCTCTGCCCGGGTCTCAACACCGTCGTCCGAGAGCTCGTTCTTGGCCTCTGGGATCTCTACGGAGTCCGCCACATTTTTGGCATCAAGGCTGGCTACCGTGGCTTTTACTCTACGGAACCCCTCCCACTCAACCCCAAGCTTGTTCACAACTGGCACAAGAACGGTGGCACCCATCTCCAGACCTCCAGAGGTGGTTTTGATCTTCAAAAGATTGTCGATGCCATCCAACATCGCGGCTATAATCAGTTGTACATCATAGGTGGAGATGGCACGATGCGCGGGGCTGTTGAGATATATCATGAGATATGTCAGCGCAAACTGAAAATTGCGGTGACTGGAATTCCAAAAACTGTGGACAATGATGTAGGTATCATTGACAGGTCTTTTGGGTTCCAGACAGCGGTAGAAATGGCGCAGCAGGCAATCAGCGCAGCTCATGTGGAGGCGGAAAGTGCAGTGAATGGAATAGGGTTGGTGAAGCTAATGGGTCGAAGTACAGGGCATATTGCTCTACATGCAACACTGAGCAGCCGTGACGTGGACTGCTGCCTAATTCCTGAAACTCCATTTTACCTGGAAGGAAAAGGAGGGCTATTTGAGTTCCTTGAGCAGCGATTGAGGGAGAATGGACACGCAGTGTTAGTAGTGGCTGAGGGAGCTGGACAGGATCAAATGATCGAGGCTAATAACAATGATCATCATGCTGATAATAATAAGGATGAATCAGGGAACCCAGTGTTCCTTGACGTTGGGCAGTGGTTGAAGTCGGAGCTAAACAAGTGGTGGAAGAGGGAGCACCCAAATGAGCTCTTCACTGTCAAGTACATAGATCCGACATACATGATACGCGCGGTTGCTGCAAATTCAACAGATAACTTGTACTGCACACTGGTAGCGCATTCAGCAATCCACGGAGTCATGGCAGGGTATACTGGATTTGTGTCTGGCCCCATCAACGGAAACTATGCTTACATTCCACTGGAAGAGGTTGCCAAGGCTAAAAATGAAGTCGACACGAGAGACCACAAATGGGCCTGGGTTAGATCGGTCACCAACCAGCCTGATTTTCATATATACAGCAACGACGACAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

517

Amino Acids

57.01

Weight (kDa)

6.08

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 144 - 455 2.5e-55 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 2 cut(s) 302, 452
AccB1I GGYRCC 1 cut(s) 620
AccI GTMKAC 2 cut(s) 1382, 1464
AccII CGCG 5 cut(s) 384, 687, 732, 1302, 1304
AclWI GGATC 5 cut(s) 157, 431, 513, 1128, 1277
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 792, 1315
AcyI GRCGYC 1 cut(s) 397
AdeI CACNNNGTG 1 cut(s) 1090
AfaI GTAC 5 cut(s) 355, 704, 949, 1277, 1334
AfiI CCNNNNNNNGG 5 cut(s) 460, 853, 991, 1409, 1483
AgsI TTSAA 4 cut(s) 170, 659, 1216, 1320
AhlI ACTAGT 1 cut(s) 362
AjiI CACGTC 1 cut(s) 991
AjnI CCWGG 3 cut(s) 419, 1026, 1487
AluBI AGCT 9 cut(s) 109, 302, 487, 592, 887, 934, 1112, 1225, 1261
AluI AGCT 9 cut(s) 109, 302, 487, 592, 887, 934, 1112, 1225, 1261
Alw21I GWGCWC 3 cut(s) 489, 1251, 1263
Alw26I GTCTC 2 cut(s) 470, 1467
AlwI GGATC 5 cut(s) 157, 431, 513, 1128, 1277
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 7 cut(s) 3, 388, 451, 496, 1399, 1485, 1545
ApoI RAATTY 2 cut(s) 792, 1315
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
AspLEI GCGC 8 cut(s) 13, 386, 732, 768, 868, 884, 1304, 1351
AspS9I GGNCC 3 cut(s) 389, 1400, 1485
AsuC2I CCSGG 2 cut(s) 461, 462
AsuHPI GGTGA 5 cut(s) 85, 338, 796, 940, 1495
AvaI CYCGRG 1 cut(s) 460
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 2 cut(s) 489, 1263
BauI CACGAG 1 cut(s) 1468
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 3 cut(s) 489, 1251, 1263
BbvCI CCTCAGC 1 cut(s) 1104
BccI CCATC 5 cut(s) 253, 633, 683, 713, 1412
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 3 cut(s) 421, 1028, 1489
BclI TGATCA 2 cut(s) 133, 1147
BcnI CCSGG 2 cut(s) 461, 462
BcoDI GTCTC 2 cut(s) 470, 1467
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 4 cut(s) 164, 197, 351, 363
BfmI CTRYAG 1 cut(s) 303
BfuAI ACCTGC 2 cut(s) 302, 452
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 5 cut(s) 421, 461, 462, 1028, 1489
BmeT110I CYCGRG 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 991
BmgT120I GGNCC 3 cut(s) 389, 1400, 1485
BmiI GGNNCC 5 cut(s) 570, 622, 845, 1184, 1402
BmrFI CCNGG 5 cut(s) 421, 461, 462, 1028, 1489
BmrI ACTGGG 2 cut(s) 214, 1181
BmsI GCATC 4 cut(s) 403, 543, 661, 717
BmuI ACTGGG 2 cut(s) 214, 1181
BpiI GAAGAC 1 cut(s) 52
BpmI CTGGAG 3 cut(s) 95, 614, 622
Bpu10I CCTNAGC 1 cut(s) 1104
BpuEI CTTGAG 1 cut(s) 1079
BpuMI CCSGG 2 cut(s) 461, 462
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 397
BsaI GGTCTC 2 cut(s) 470, 1467
BsaJI CCNNGG 7 cut(s) 6, 369, 460, 550, 1363, 1446, 1488
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 5 cut(s) 460, 853, 991, 1409, 1483
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 7 cut(s) 220, 608, 793, 1187, 1347, 1390, 1437
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 3 cut(s) 421, 1028, 1489
BseDI CCNNGG 7 cut(s) 6, 369, 460, 550, 1363, 1446, 1488
BseGI GGATG 2 cut(s) 675, 1177
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 5 cut(s) 460, 853, 991, 1409, 1483
BseMII CTCAG 2 cut(s) 968, 1095
BseNI ACTGG 7 cut(s) 220, 608, 793, 1187, 1347, 1390, 1437
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 927, 1321
Bsh1236I CGCG 5 cut(s) 384, 687, 732, 1302, 1304
BshFI GGCC 7 cut(s) 5, 390, 453, 498, 1401, 1487, 1547
BshNI GGYRCC 1 cut(s) 620
BsiHKAI GWGCWC 3 cut(s) 489, 1251, 1263
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 2 cut(s) 387, 461
BslI CCNNNNNNNGG 5 cut(s) 460, 853, 991, 1409, 1483
BsmAI GTCTC 2 cut(s) 470, 1467
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 7 cut(s) 5, 390, 453, 498, 1401, 1487, 1547
Bso31I GGTCTC 2 cut(s) 470, 1467
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 3 cut(s) 489, 1251, 1263
Bsp1407I TGTACA 1 cut(s) 702
BspANI GGCC 7 cut(s) 5, 390, 453, 498, 1401, 1487, 1547
BspCNI CTCAG 2 cut(s) 969, 1096
BspFNI CGCG 5 cut(s) 384, 687, 732, 1302, 1304
BspHI TCATGA 1 cut(s) 751
BspLI GGNNCC 5 cut(s) 570, 622, 845, 1184, 1402
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 2 cut(s) 302, 452
BspPI GGATC 5 cut(s) 157, 431, 513, 1128, 1277
BspQI GCTCTTC 1 cut(s) 1268
BspT107I GGYRCC 1 cut(s) 620
BspTNI GGTCTC 2 cut(s) 470, 1467
BsrFI RCCGGY 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 7 cut(s) 220, 608, 793, 1187, 1347, 1390, 1437
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 7 cut(s) 6, 369, 460, 550, 1363, 1446, 1488
BssNAI GTATAC 1 cut(s) 1383
BssNI GRCGYC 1 cut(s) 397
BssSI CACGAG 1 cut(s) 1468
BssT1I CCWWGG 2 cut(s) 369, 1446
Bst1107I GTATAC 1 cut(s) 1383
Bst2BI CACGAG 1 cut(s) 1468
Bst2UI CCWGG 3 cut(s) 421, 1028, 1489
Bst4CI ACNGT 5 cut(s) 475, 551, 617, 805, 1270
Bst6I CTCTTC 3 cut(s) 1235, 1268, 1431
BstACI GRCGYC 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 388, 544, 873
BstDEI CTNAG 2 cut(s) 977, 1104
BstDSI CCRYGG 3 cut(s) 6, 550, 1363
BstEII GGTNACC 1 cut(s) 1501
BstF5I GGATG 2 cut(s) 675, 1177
BstFNI CGCG 5 cut(s) 384, 687, 732, 1302, 1304
BstHHI GCGC 8 cut(s) 13, 386, 732, 768, 868, 884, 1304, 1351
BstMAI GTCTC 2 cut(s) 470, 1467
BstNI CCWGG 3 cut(s) 421, 1028, 1489
BstNSI RCATGY 1 cut(s) 971
BstPI GGTNACC 1 cut(s) 1501
BstSCI CCNGG 5 cut(s) 419, 459, 460, 1026, 1487
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 5 cut(s) 384, 687, 732, 1302, 1304
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 2 cut(s) 505, 1282
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 2 cut(s) 505, 1282
BstZ17I GTATAC 1 cut(s) 1383
BsuRI GGCC 7 cut(s) 5, 390, 453, 498, 1401, 1487, 1547
BtgI CCRYGG 3 cut(s) 6, 550, 1363
BtgZI GCGATG 1 cut(s) 668
BtrI CACGTC 1 cut(s) 991
BtsCI GGATG 2 cut(s) 675, 1177
BtsI GCAGTG 3 cut(s) 915, 1095, 1214
BtsIMutI CAGTG 8 cut(s) 915, 974, 1095, 1194, 1214, 1266, 1340, 1430
BveI ACCTGC 2 cut(s) 302, 452
Cac8I GCNNGC 3 cut(s) 388, 544, 873
CciI TCATGA 1 cut(s) 751
CfoI GCGC 8 cut(s) 13, 386, 732, 768, 868, 884, 1304, 1351
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 3 cut(s) 389, 1400, 1485
Cfr9I CCCGGG 1 cut(s) 460
CseI GACGC 2 cut(s) 405, 405
Csp6I GTAC 5 cut(s) 354, 703, 948, 1276, 1333
CspCI CAANNNNNGTGG 4 cut(s) 598, 633, 786, 821
CviAII CATG 7 cut(s) 50, 752, 890, 968, 1154, 1294, 1372
CviQI GTAC 5 cut(s) 354, 703, 948, 1276, 1333
DdeI CTNAG 2 cut(s) 977, 1104
DraIII CACNNNGTG 1 cut(s) 1090
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 1235, 1268, 1431
EarI CTCTTC 3 cut(s) 1235, 1268, 1431
EciI GGCGGA 3 cut(s) 251, 509, 914
Ecl136II GAGCTC 2 cut(s) 487, 1261
Eco130I CCWWGG 2 cut(s) 369, 1446
Eco147I AGGCCT 1 cut(s) 1547
Eco24I GRGCYC 2 cut(s) 489, 1263
Eco31I GGTCTC 2 cut(s) 470, 1467
Eco53kI GAGCTC 2 cut(s) 487, 1261
Eco88I CYCGRG 1 cut(s) 460
Eco91I GGTNACC 1 cut(s) 1501
EcoICRI GAGCTC 2 cut(s) 487, 1261
EcoO65I GGTNACC 1 cut(s) 1501
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 419, 1026, 1487
EcoT14I CCWWGG 2 cut(s) 369, 1446
EcoT38I GRGCYC 2 cut(s) 489, 1263
ErhI CCWWGG 2 cut(s) 369, 1446
FaeI CATG 7 cut(s) 53, 755, 893, 971, 1157, 1297, 1375
FatI CATG 7 cut(s) 49, 751, 889, 967, 1153, 1293, 1371
FauI CCCGC 1 cut(s) 725
FbaI TGATCA 2 cut(s) 133, 1147
FblI GTMKAC 2 cut(s) 1382, 1464
FokI GGATG 2 cut(s) 662, 1184
FriOI GRGCYC 2 cut(s) 489, 1263
FspBI CTAG 4 cut(s) 164, 197, 351, 363
GlaI GCGC 8 cut(s) 12, 385, 731, 767, 867, 883, 1303, 1350
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 3 cut(s) 95, 614, 622
HaeIII GGCC 7 cut(s) 5, 390, 453, 498, 1401, 1487, 1547
HapII CCGG 2 cut(s) 387, 461
HgaI GACGC 2 cut(s) 405, 405
HhaI GCGC 8 cut(s) 13, 386, 732, 768, 868, 884, 1304, 1351
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 7 cut(s) 53, 755, 893, 971, 1157, 1297, 1375
Hin6I GCGC 8 cut(s) 11, 384, 730, 766, 866, 882, 1302, 1349
HinP1I GCGC 8 cut(s) 11, 384, 730, 766, 866, 882, 1302, 1349
HincII GTYRAC 1 cut(s) 1465
HindII GTYRAC 1 cut(s) 1465
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 516, 1175, 1368
HpaII CCGG 2 cut(s) 387, 461
HphI GGTGA 5 cut(s) 85, 338, 796, 940, 1495
Hpy166II GTNNAC 5 cut(s) 598, 808, 994, 1383, 1465
Hpy188I TCNGA 7 cut(s) 149, 154, 226, 250, 482, 1222, 1287
Hpy188III TCNNGA 6 cut(s) 112, 631, 639, 752, 847, 1010
Hpy8I GTNNAC 5 cut(s) 598, 808, 994, 1383, 1465
Hpy99I CGWCG 3 cut(s) 399, 479, 1541
HpyAV CCTTC 3 cut(s) 100, 220, 1025
HpyCH4III ACNGT 5 cut(s) 475, 551, 617, 805, 1270
HpyCH4IV ACGT 2 cut(s) 990, 1200
HpyCH4V TGCA 5 cut(s) 305, 908, 971, 1313, 1338
HpyF3I CTNAG 2 cut(s) 977, 1104
HpySE526I ACGT 2 cut(s) 990, 1200
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 7 cut(s) 53, 755, 893, 971, 1157, 1297, 1375
HspAI GCGC 8 cut(s) 11, 384, 730, 766, 866, 882, 1302, 1349
KroI GCCGGC 1 cut(s) 386
KroNI GCCGGC 1 cut(s) 388
Ksp22I TGATCA 2 cut(s) 133, 1147
LguI GCTCTTC 1 cut(s) 1268
LmnI GCTCC 4 cut(s) 114, 1109, 1222, 1246
LweI GCATC 4 cut(s) 403, 543, 661, 717
MaeI CTAG 4 cut(s) 164, 197, 351, 363
MaeII ACGT 2 cut(s) 990, 1200
MaeIII GTNAC 5 cut(s) 173, 439, 784, 986, 1501
MboII GAAGA 9 cut(s) 57, 77, 205, 341, 344, 647, 1252, 1255, 1448
MflI RGATCY 2 cut(s) 505, 1282
MhlI GDGCHC 3 cut(s) 489, 1251, 1263
MluCI AATT 4 cut(s) 777, 792, 1005, 1315
MlyI GAGTC 2 cut(s) 525, 1377
MmeI TCCRAC 3 cut(s) 703, 1200, 1310
MroNI GCCGGC 1 cut(s) 386
MslI CAYNNNNRTG 2 cut(s) 54, 1254
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 2 cut(s) 387, 461
MspR9I CCNGG 5 cut(s) 421, 461, 462, 1028, 1489
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 3 cut(s) 421, 1028, 1489
MvnI CGCG 5 cut(s) 384, 687, 732, 1302, 1304
NaeI GCCGGC 1 cut(s) 388
NciI CCSGG 2 cut(s) 461, 462
NgoMIV GCCGGC 1 cut(s) 386
NlaIII CATG 7 cut(s) 53, 755, 893, 971, 1157, 1297, 1375
NlaIV GGNNCC 5 cut(s) 570, 622, 845, 1184, 1402
NmuCI GTSAC 4 cut(s) 439, 784, 986, 1501
NspI RCATGY 1 cut(s) 971
PagI TCATGA 1 cut(s) 751
PaqCI CACCTGC 1 cut(s) 302
PceI AGGCCT 1 cut(s) 1547
PciSI GCTCTTC 1 cut(s) 1268
PctI GAATGC 1 cut(s) 1351
PdiI GCCGGC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 419
PleI GAGTC 2 cut(s) 524, 1376
PpsI GAGTC 2 cut(s) 524, 1376
Psp124BI GAGCTC 2 cut(s) 489, 1263
Psp6I CCWGG 3 cut(s) 419, 1026, 1487
PspEI GGTNACC 1 cut(s) 1501
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 3 cut(s) 419, 1026, 1487
PspN4I GGNNCC 5 cut(s) 570, 622, 845, 1184, 1402
PspPI GGNCC 3 cut(s) 389, 1400, 1485
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 505, 1282
RsaI GTAC 5 cut(s) 355, 704, 949, 1277, 1334
RsaNI GTAC 5 cut(s) 354, 703, 948, 1276, 1333
RseI CAYNNNNRTG 2 cut(s) 54, 1254
SacI GAGCTC 2 cut(s) 489, 1263
SalI GTCGAC 1 cut(s) 1463
SapI GCTCTTC 1 cut(s) 1268
Sau96I GGNCC 3 cut(s) 389, 1400, 1485
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 2 cut(s) 525, 1377
ScrFI CCNGG 5 cut(s) 421, 461, 462, 1028, 1489
SduI GDGCHC 3 cut(s) 489, 1251, 1263
SfaNI GCATC 4 cut(s) 403, 543, 661, 717
SfcI CTRYAG 1 cut(s) 303
SmaI CCCGGG 1 cut(s) 462
SmiMI CAYNNNNRTG 2 cut(s) 54, 1254
SmlI CTYRAG 1 cut(s) 1058
SmoI CTYRAG 1 cut(s) 1058
SpeI ACTAGT 1 cut(s) 362
Sse9I AATT 4 cut(s) 777, 792, 1005, 1315
SseBI AGGCCT 1 cut(s) 1547
SspMI CTAG 4 cut(s) 164, 197, 351, 363
SstI GAGCTC 2 cut(s) 489, 1263
StuI AGGCCT 1 cut(s) 1547
StyD4I CCNGG 5 cut(s) 419, 459, 460, 1026, 1487
StyI CCWWGG 2 cut(s) 369, 1446
TaaI ACNGT 5 cut(s) 475, 551, 617, 805, 1270
TaiI ACGT 2 cut(s) 993, 1203
TaqI TCGA 5 cut(s) 411, 669, 943, 1131, 1464
TaqII GACCGA 1 cut(s) 1489
TasI AATT 4 cut(s) 777, 792, 1005, 1315
TatI WGTACW 5 cut(s) 353, 702, 947, 1275, 1332
TauI GCSGC 2 cut(s) 434, 690
TfiI GAWTC 1 cut(s) 1175
TscAI CASTG 8 cut(s) 915, 981, 1095, 1194, 1214, 1273, 1347, 1437
TseFI GTSAC 4 cut(s) 439, 784, 986, 1501
Tsp45I GTSAC 4 cut(s) 439, 784, 986, 1501
TspDTI ATGAA 4 cut(s) 78, 1188, 1473, 1511
TspGWI ACGGA 4 cut(s) 528, 581, 1380, 1425
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 8 cut(s) 915, 981, 1095, 1194, 1214, 1273, 1347, 1437
XapI RAATTY 2 cut(s) 792, 1315
XceI RCATGY 1 cut(s) 971
XmaI CCCGGG 1 cut(s) 460
XmiI GTMKAC 2 cut(s) 1382, 1464
XspI CTAG 4 cut(s) 164, 197, 351, 363
ZrmI AGTACT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.