Rroxscaffold_1G00075860

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014539
Physical Location & Seq
Reverse (-)
96436507 .. 96438704
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075860.1

Sequence Viewer

Length: 1395 bp
ATGGCAGAACCCACAACAGAGGAGATGATCTTGTCATTGTCTCCGGTGAAGCTCCACAGACTACCCCACCTGACCGACTATCTCTCACAACTCCCCACCCACACCAACCCACTCCAACACAACCCCTTCTACCACCCTTCCGACGGCTTCTACATAACCCCATCCGATGTCATCCTCCGCCAGATTGTCTACGACCTCGCCGCCTCCGGCTCCGGCGCGGCCTCCGCTCATCCCGCCTCAGCTTACCATCGCGCCGGCCCACGACGGCACATCTTGTTTGAGCCGTCGAGCATCAGGGCGGCTATTGTGACATGCGGTGGGCTGTGCCCGGGCATGAACACGGTGGTGAGAGAGCTGGTTCTTGGCCTCTGGGACCTCTACGGAGTCCGTCAGATTTTTGGTATCAAGGCTGGCTATCGTGGATTTTACTCTATGGATCCCGTCCAGCTTAACCCAAAGCTCGTCAACCACTGGCACAAGAATGGCGGCACTGTCCTCGAGACCTCCAGAGGTGGTTTTCATCTTCAAAAGATTGTCGATGCCATCCAACATCGCGGCTATAATCAGGTGTACATTATAGGTGGAGATGGCACAATGCGGGGGGCGGTTGAGATATTTCATGAGATTTGTAGGCGAAAACTGAACATTGCGGTTACTGGGATTCCAAAAACAGTTGACAATGATGTTGGCATCATTGACAGATCTTTCGGATTCCAGACAGCAGTAGAAATGGCCCAGCAGGCAATCAGTGCAGCTCATGTGGAGGCTGAAAGTGCAGTGAATGGAATAGGCTTGGTCAAGTTGATGGGTCGAAGTACGGGCCACATTGCTCTCTATGCAACATTGAGCAGCCGAGACGTGGATTGCTGCCTAATCCCAGAAAATGAGTTTTACCTGGAAGGAAAAGGAGGGCTATTTGAATTCCTTGAGCAGCGACTGAGGGAGAATGGACACGCAGTGTTAGTTGTGGCTGAAGGAGCGGGACAGGACATAATTCCAAGGAACGAGGCTCAGAAACAGGAGAGGGATGAATCTGGAAACCCAGTGTTCCTAGATGTTGGCCAATGGTTGAAGTCAGAGCTCAATAATTGGTGGAGCAGGGATCACCCCAATGAGCTCTTCACAGTCAAGTACATAGATCCTACATACATGATACGGGCCGTCCCTGCAAATGCTACGGATAACTTGTACTGTACACTTCTTGCCCATTCAGCTATTCATGGAGTGATGGCTGGTTACACTGGATTCGTCCCTGGCCCTATCAATGGCAATTATGCTTACATTCCAGTTGAAGAGGTAGCAAAGGCTAAGAATGAGGTCAACACGAGAGACCACCAATGGGCGTGGGTCAGATCTGTCACAAACCAGCCTGATTTTATGAGGACTATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

464

Amino Acids

51.44

Weight (kDa)

6.2

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 99 - 407 5.3e-55 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 227, 980
AccI GTMKAC 1 cut(s) 189
AccII CGCG 3 cut(s) 218, 252, 555
AclWI GGATC 4 cut(s) 431, 444, 1110, 1133
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 1 cut(s) 920
AcuI CTGAAG 1 cut(s) 993
AdeI CACNNNGTG 1 cut(s) 958
AfaI GTAC 5 cut(s) 572, 817, 1133, 1190, 1195
AfiI CCNNNNNNNGG 4 cut(s) 143, 859, 1265, 1339
AgsI TTSAA 4 cut(s) 527, 920, 1072, 1292
AjiI CACGTC 1 cut(s) 859
AjnI CCWGG 2 cut(s) 894, 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 1103, 1135
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 9 cut(s) 52, 242, 355, 448, 460, 755, 1081, 1117, 1214
AluI AGCT 9 cut(s) 52, 242, 355, 448, 460, 755, 1081, 1117, 1214
Alw21I GWGCWC 2 cut(s) 1083, 1119
Alw26I GTCTC 4 cut(s) 45, 494, 849, 1323
AlwI GGATC 4 cut(s) 431, 444, 1110, 1133
AlwNI CAGNNNCTG 1 cut(s) 937
Ama87I CYCGRG 2 cut(s) 328, 497
AoxI GGCC 8 cut(s) 219, 256, 364, 732, 820, 1060, 1158, 1255
ApeKI GCWGC 4 cut(s) 752, 849, 867, 931
ApoI RAATTY 1 cut(s) 920
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AspLEI GCGC 2 cut(s) 218, 254
AspS9I GGNCC 6 cut(s) 257, 373, 733, 820, 1158, 1256
AsuC2I CCSGG 2 cut(s) 329, 330
AsuHPI GGTGA 3 cut(s) 58, 358, 1097
AvaI CYCGRG 2 cut(s) 328, 497
AvaII GGWCC 1 cut(s) 373
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 1062
BamHI GGATCC 1 cut(s) 436
BanII GRGCYC 2 cut(s) 1083, 1119
BauI CACGAG 1 cut(s) 1324
Bbv12I GWGCWC 2 cut(s) 1083, 1119
BbvCI CCTCAGC 1 cut(s) 238
BbvI GCAGC 4 cut(s) 764, 854, 861, 943
BccI CCATC 6 cut(s) 169, 255, 551, 581, 799, 1222
BceAI ACGGC 4 cut(s) 160, 268, 281, 1145
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 896, 1254
BcnI CCSGG 2 cut(s) 329, 330
BcoDI GTCTC 4 cut(s) 45, 494, 849, 1323
BfaI CTAG 1 cut(s) 1052
BglI GCCNNNNNGGC 1 cut(s) 740
BglII AGATCT 2 cut(s) 701, 1352
BisI GCNGC 9 cut(s) 201, 219, 300, 487, 556, 753, 850, 868, 932
BlsI GCNGC 9 cut(s) 202, 220, 301, 488, 557, 754, 851, 869, 933
Bme1390I CCNGG 4 cut(s) 329, 330, 896, 1254
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 2 cut(s) 328, 497
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 6 cut(s) 257, 373, 733, 820, 1158, 1256
BmiI GGNNCC 3 cut(s) 211, 374, 438
BmrFI CCNGG 4 cut(s) 329, 330, 896, 1254
BmrI ACTGGG 2 cut(s) 666, 1037
BmsI GCATC 3 cut(s) 300, 529, 699
BmuI ACTGGG 2 cut(s) 666, 1037
BpmI CTGGAG 1 cut(s) 490
Bpu10I CCTNAGC 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 947
BpuMI CCSGG 2 cut(s) 329, 330
BsaI GGTCTC 2 cut(s) 494, 1323
BsaJI CCNNGG 3 cut(s) 328, 998, 1252
BsaWI WCCGGW 1 cut(s) 43
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 859, 1265, 1339
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 5 cut(s) 476, 661, 1043, 1246, 1286
Bse3DI GCAATG 2 cut(s) 645, 825
BseBI CCWGG 2 cut(s) 896, 1254
BseDI CCNNGG 3 cut(s) 328, 998, 1252
BseGI GGATG 5 cut(s) 161, 171, 229, 543, 1033
BseLI CCNNNNNNNGG 4 cut(s) 143, 859, 1265, 1339
BseMI GCAATG 2 cut(s) 645, 825
BseMII CTCAG 3 cut(s) 252, 929, 1025
BseNI ACTGG 5 cut(s) 476, 661, 1043, 1246, 1286
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 4 cut(s) 764, 854, 861, 943
BseYI CCCAGC 1 cut(s) 735
BsgI GTGCAG 2 cut(s) 771, 795
Bsh1236I CGCG 3 cut(s) 218, 252, 555
BshFI GGCC 8 cut(s) 221, 258, 366, 734, 822, 1062, 1160, 1257
BsiHKAI GWGCWC 2 cut(s) 1083, 1119
BsiHKCI CYCGRG 2 cut(s) 328, 497
BsiSI CCGG 5 cut(s) 44, 207, 213, 255, 329
BslFI GGGAC 4 cut(s) 386, 996, 1148, 1235
BslI CCNNNNNNNGG 4 cut(s) 143, 859, 1265, 1339
BsmAI GTCTC 4 cut(s) 45, 494, 849, 1323
BsmBI CGTCTC 1 cut(s) 849
BsmFI GGGAC 4 cut(s) 386, 996, 1148, 1235
BsnI GGCC 8 cut(s) 221, 258, 366, 734, 822, 1062, 1160, 1257
Bso31I GGTCTC 2 cut(s) 494, 1323
BsoBI CYCGRG 2 cut(s) 328, 497
Bsp1286I GDGCHC 3 cut(s) 329, 1083, 1119
Bsp1407I TGTACA 2 cut(s) 570, 1193
Bsp143I GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
BspANI GGCC 8 cut(s) 221, 258, 366, 734, 822, 1062, 1160, 1257
BspCNI CTCAG 3 cut(s) 251, 930, 1024
BspFNI CGCG 3 cut(s) 218, 252, 555
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 3 cut(s) 211, 374, 438
BspPI GGATC 4 cut(s) 431, 444, 1110, 1133
BspQI GCTCTTC 1 cut(s) 1124
BspTNI GGTCTC 2 cut(s) 494, 1323
BsrBI CCGCTC 2 cut(s) 227, 980
BsrDI GCAATG 2 cut(s) 645, 825
BsrFI RCCGGY 1 cut(s) 254
BsrGI TGTACA 2 cut(s) 570, 1193
BsrI ACTGG 5 cut(s) 476, 661, 1043, 1246, 1286
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 3 cut(s) 328, 998, 1252
BssMI GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
BssSI CACGAG 1 cut(s) 1324
BssT1I CCWWGG 1 cut(s) 998
Bst2BI CACGAG 1 cut(s) 1324
Bst2UI CCWGG 2 cut(s) 896, 1254
Bst4CI ACNGT 5 cut(s) 343, 493, 673, 1126, 1193
Bst6I CTCTTC 2 cut(s) 1124, 1287
BstAPI GCANNNNNTGC 1 cut(s) 749
BstAUI TGTACA 2 cut(s) 570, 1193
BstC8I GCNNGC 3 cut(s) 256, 412, 741
BstDEI CTNAG 4 cut(s) 238, 938, 1011, 1308
BstF5I GGATG 5 cut(s) 161, 171, 229, 543, 1033
BstFNI CGCG 3 cut(s) 218, 252, 555
BstHHI GCGC 2 cut(s) 218, 254
BstKTI GATC 6 cut(s) 30, 439, 704, 1105, 1141, 1355
BstMAI GTCTC 4 cut(s) 45, 494, 849, 1323
BstMBI GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
BstMWI GCNNNNNNNGC 9 cut(s) 224, 233, 740, 749, 773, 836, 977, 1166, 1211
BstNI CCWGG 2 cut(s) 896, 1254
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 4 cut(s) 327, 328, 894, 1252
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 3 cut(s) 218, 252, 555
BstV1I GCAGC 4 cut(s) 764, 854, 861, 943
BstX2I RGATCY 4 cut(s) 436, 701, 1138, 1352
BstYI RGATCY 4 cut(s) 436, 701, 1138, 1352
BsuRI GGCC 8 cut(s) 221, 258, 366, 734, 822, 1062, 1160, 1257
BtgZI GCGATG 2 cut(s) 233, 536
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 5 cut(s) 161, 171, 229, 543, 1033
BtsI GCAGTG 2 cut(s) 783, 963
BtsIMutI CAGTG 7 cut(s) 469, 489, 754, 783, 963, 1050, 1239
Cac8I GCNNGC 3 cut(s) 256, 412, 741
CaiI CAGNNNCTG 1 cut(s) 937
CciI TCATGA 1 cut(s) 619
CfoI GCGC 2 cut(s) 218, 254
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 6 cut(s) 257, 373, 733, 820, 1158, 1256
Cfr9I CCCGGG 1 cut(s) 328
Csp6I GTAC 5 cut(s) 571, 816, 1132, 1189, 1194
CspCI CAANNNNNGTGG 2 cut(s) 1325, 1360
CviAII CATG 6 cut(s) 312, 334, 620, 758, 1150, 1220
CviQI GTAC 5 cut(s) 571, 816, 1132, 1189, 1194
DdeI CTNAG 4 cut(s) 238, 938, 1011, 1308
DpnI GATC 6 cut(s) 29, 438, 703, 1104, 1140, 1354
DpnII GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
DraIII CACNNNGTG 1 cut(s) 958
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 2 cut(s) 1124, 1287
EarI CTCTTC 2 cut(s) 1124, 1287
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 1081, 1117
Eco130I CCWWGG 1 cut(s) 998
Eco24I GRGCYC 2 cut(s) 1083, 1119
Eco31I GGTCTC 2 cut(s) 494, 1323
Eco47I GGWCC 1 cut(s) 373
Eco53kI GAGCTC 2 cut(s) 1081, 1117
Eco57I CTGAAG 1 cut(s) 993
Eco88I CYCGRG 2 cut(s) 328, 497
EcoICRI GAGCTC 2 cut(s) 1081, 1117
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 920
EcoRII CCWGG 2 cut(s) 894, 1252
EcoT14I CCWWGG 1 cut(s) 998
EcoT38I GRGCYC 2 cut(s) 1083, 1119
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 849
FaeI CATG 6 cut(s) 315, 337, 623, 761, 1153, 1223
FaqI GGGAC 4 cut(s) 386, 996, 1148, 1235
FatI CATG 6 cut(s) 311, 333, 619, 757, 1149, 1219
FauI CCCGC 3 cut(s) 241, 591, 973
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 9 cut(s) 201, 219, 300, 487, 556, 753, 850, 868, 932
FokI GGATG 5 cut(s) 148, 158, 216, 530, 1040
FriOI GRGCYC 2 cut(s) 1083, 1119
Fsp4HI GCNGC 9 cut(s) 201, 219, 300, 487, 556, 753, 850, 868, 932
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 2 cut(s) 217, 253
GluI GCNGC 9 cut(s) 201, 219, 300, 487, 556, 753, 850, 868, 932
GsaI CCCAGC 1 cut(s) 739
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 8 cut(s) 221, 258, 366, 734, 822, 1062, 1160, 1257
HapII CCGG 5 cut(s) 44, 207, 213, 255, 329
HhaI GCGC 2 cut(s) 218, 254
Hin1II CATG 6 cut(s) 315, 337, 623, 761, 1153, 1223
Hin6I GCGC 2 cut(s) 216, 252
HinP1I GCGC 2 cut(s) 216, 252
HincII GTYRAC 3 cut(s) 466, 676, 1321
HindII GTYRAC 3 cut(s) 466, 676, 1321
HinfI GANTC 5 cut(s) 384, 661, 711, 1031, 1245
HpaII CCGG 5 cut(s) 44, 207, 213, 255, 329
HphI GGTGA 3 cut(s) 58, 358, 1097
Hpy166II GTNNAC 6 cut(s) 190, 466, 571, 676, 1196, 1321
Hpy188I TCNGA 7 cut(s) 142, 166, 393, 710, 1014, 1078, 1352
Hpy188III TCNNGA 5 cut(s) 499, 507, 620, 715, 1035
Hpy8I GTNNAC 6 cut(s) 190, 466, 571, 676, 1196, 1321
Hpy99I CGWCG 3 cut(s) 146, 267, 289
HpyAV CCTTC 4 cut(s) 136, 147, 893, 968
HpyCH4III ACNGT 5 cut(s) 343, 493, 673, 1126, 1193
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 4 cut(s) 752, 776, 839, 1169
HpyF10VI GCNNNNNNNGC 9 cut(s) 224, 233, 740, 749, 773, 836, 977, 1166, 1211
HpyF3I CTNAG 4 cut(s) 238, 938, 1011, 1308
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 6 cut(s) 315, 337, 623, 761, 1153, 1223
HspAI GCGC 2 cut(s) 216, 252
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
LguI GCTCTTC 1 cut(s) 1124
LmnI GCTCC 4 cut(s) 57, 215, 977, 1095
Lsp1109I GCAGC 4 cut(s) 764, 854, 861, 943
LweI GCATC 3 cut(s) 300, 529, 699
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 4 cut(s) 307, 652, 1235, 1357
MalI GATC 6 cut(s) 29, 438, 703, 1104, 1140, 1354
MbiI CCGCTC 2 cut(s) 227, 980
MboI GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
MboII GAAGA 3 cut(s) 515, 1111, 1304
MflI RGATCY 4 cut(s) 436, 701, 1138, 1352
MhlI GDGCHC 3 cut(s) 329, 1083, 1119
MlsI TGGCCA 1 cut(s) 1062
MluCI AATT 4 cut(s) 920, 993, 1087, 1270
MluNI TGGCCA 1 cut(s) 1062
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 3 cut(s) 139, 165, 571
Mox20I TGGCCA 1 cut(s) 1062
MroNI GCCGGC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 3 cut(s) 344, 480, 1110
Msp20I TGGCCA 1 cut(s) 1062
MspI CCGG 5 cut(s) 44, 207, 213, 255, 329
MspR9I CCNGG 4 cut(s) 329, 330, 896, 1254
MvaI CCWGG 2 cut(s) 896, 1254
MvnI CGCG 3 cut(s) 218, 252, 555
MwoI GCNNNNNNNGC 9 cut(s) 224, 233, 740, 749, 773, 836, 977, 1166, 1211
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 2 cut(s) 329, 330
NdeII GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 6 cut(s) 315, 337, 623, 761, 1153, 1223
NlaIV GGNNCC 3 cut(s) 211, 374, 438
NmeAIII GCCGAG 1 cut(s) 878
NmuCI GTSAC 2 cut(s) 307, 1357
NspI RCATGY 1 cut(s) 315
OliI CACNNNNGTG 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 4 cut(s) 661, 711, 1031, 1245
PkrI GCNGC 9 cut(s) 202, 220, 301, 488, 557, 754, 851, 869, 933
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
PpuMI RGGWCCY 1 cut(s) 373
Psp124BI GAGCTC 2 cut(s) 1083, 1119
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 894, 1252
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 2 cut(s) 894, 1252
PspN4I GGNNCC 3 cut(s) 211, 374, 438
PspPI GGNCC 6 cut(s) 257, 373, 733, 820, 1158, 1256
PspPPI RGGWCCY 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 937
PsuI RGATCY 4 cut(s) 436, 701, 1138, 1352
RsaI GTAC 5 cut(s) 572, 817, 1133, 1190, 1195
RsaNI GTAC 5 cut(s) 571, 816, 1132, 1189, 1194
RseI CAYNNNNRTG 3 cut(s) 344, 480, 1110
SacI GAGCTC 2 cut(s) 1083, 1119
SapI GCTCTTC 1 cut(s) 1124
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 9 cut(s) 201, 219, 300, 487, 556, 753, 850, 868, 932
Sau3AI GATC 6 cut(s) 27, 436, 701, 1102, 1138, 1352
Sau96I GGNCC 6 cut(s) 257, 373, 733, 820, 1158, 1256
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 4 cut(s) 329, 330, 896, 1254
SduI GDGCHC 3 cut(s) 329, 1083, 1119
SfaNI GCATC 3 cut(s) 300, 529, 699
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 497
SmaI CCCGGG 1 cut(s) 330
SmiMI CAYNNNNRTG 3 cut(s) 344, 480, 1110
SmlI CTYRAG 2 cut(s) 497, 926
SmoI CTYRAG 2 cut(s) 497, 926
SrfI GCCCGGGC 1 cut(s) 330
Sse9I AATT 4 cut(s) 920, 993, 1087, 1270
SspMI CTAG 1 cut(s) 1052
SstI GAGCTC 2 cut(s) 1083, 1119
StyD4I CCNGG 4 cut(s) 327, 328, 894, 1252
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 5 cut(s) 343, 493, 673, 1126, 1193
TaiI ACGT 1 cut(s) 861
TaqI TCGA 4 cut(s) 287, 498, 537, 811
TaqII GACCGA 1 cut(s) 89
TasI AATT 4 cut(s) 920, 993, 1087, 1270
TatI WGTACW 4 cut(s) 570, 1131, 1188, 1193
TauI GCSGC 5 cut(s) 203, 221, 302, 489, 558
TfiI GAWTC 4 cut(s) 661, 711, 1031, 1245
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 7 cut(s) 476, 496, 754, 783, 963, 1050, 1246
TseFI GTSAC 2 cut(s) 307, 1357
TseI GCWGC 4 cut(s) 752, 849, 867, 931
Tsp45I GTSAC 2 cut(s) 307, 1357
TspDTI ATGAA 5 cut(s) 350, 509, 608, 1044, 1208
TspGWI ACGGA 3 cut(s) 377, 396, 1193
TspMI CCCGGG 1 cut(s) 328
TspRI CASTG 7 cut(s) 476, 496, 754, 783, 963, 1050, 1246
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 920
XceI RCATGY 1 cut(s) 315
XhoI CTCGAG 1 cut(s) 497
XmaI CCCGGG 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.