Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G47810 |
| fragaria_vesca | FvH4_3g00180 |
| malus_domestica | MD05G1363600.v1.1 |
| prunus_persica | Prupe.4G002000_v2.0.a1 |
| pyrus_communis | pycom05g32980 |
| rosa_chinensis | RchiOBHm_Chr5g0000241 |
| rosa_laevigata | RLG00000030852 |
| rosa_multiflora | Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00075860 |
| rosa_rugosa | Rorug04G0382700 |
| rosa_samantha | Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000 |
| rosa_wichuraiana | Rw5G000220 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 293 |
| Acc36I | ACCTGC | 3 cut(s) | 293, 443, 1358 |
| AccB1I | GGYRCC | 1 cut(s) | 611 |
| AccI | GTMKAC | 1 cut(s) | 1455 |
| AccII | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| AclWI | GGATC | 5 cut(s) | 148, 422, 504, 1119, 1268 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 2 cut(s) | 783, 1306 |
| AcyI | GRCGYC | 1 cut(s) | 388 |
| AdeI | CACNNNGTG | 1 cut(s) | 1081 |
| AfaI | GTAC | 5 cut(s) | 346, 695, 940, 1268, 1325 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 451, 982, 1400, 1474 |
| AgsI | TTSAA | 4 cut(s) | 161, 650, 1207, 1311 |
| AhlI | ACTAGT | 1 cut(s) | 353 |
| AjiI | CACGTC | 1 cut(s) | 982 |
| AjnI | CCWGG | 3 cut(s) | 410, 1017, 1478 |
| AleI | CACNNNNGTG | 1 cut(s) | 1334 |
| AluBI | AGCT | 8 cut(s) | 293, 478, 583, 878, 925, 1103, 1216, 1252 |
| AluI | AGCT | 8 cut(s) | 293, 478, 583, 878, 925, 1103, 1216, 1252 |
| Alw21I | GWGCWC | 2 cut(s) | 1242, 1254 |
| Alw26I | GTCTC | 2 cut(s) | 461, 1458 |
| AlwI | GGATC | 5 cut(s) | 148, 422, 504, 1119, 1268 |
| Ama87I | CYCGRG | 1 cut(s) | 451 |
| AoxI | GGCC | 6 cut(s) | 3, 379, 442, 487, 1390, 1476 |
| ApoI | RAATTY | 2 cut(s) | 783, 1306 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 812, 844 |
| AspLEI | GCGC | 6 cut(s) | 13, 377, 723, 759, 875, 1295 |
| AspS9I | GGNCC | 4 cut(s) | 380, 508, 1391, 1476 |
| AsuC2I | CCSGG | 2 cut(s) | 452, 453 |
| AsuHPI | GGTGA | 5 cut(s) | 76, 329, 787, 931, 1486 |
| AvaI | CYCGRG | 1 cut(s) | 451 |
| AvaII | GGWCC | 1 cut(s) | 508 |
| BanI | GGYRCC | 1 cut(s) | 611 |
| BanII | GRGCYC | 1 cut(s) | 1254 |
| BauI | CACGAG | 1 cut(s) | 1459 |
| BbsI | GAAGAC | 1 cut(s) | 46 |
| Bbv12I | GWGCWC | 2 cut(s) | 1242, 1254 |
| BbvCI | CCTCAGC | 1 cut(s) | 1095 |
| BccI | CCATC | 5 cut(s) | 244, 624, 674, 704, 1403 |
| BceAI | ACGGC | 2 cut(s) | 622, 960 |
| BciT130I | CCWGG | 3 cut(s) | 412, 1019, 1480 |
| BclI | TGATCA | 2 cut(s) | 124, 1138 |
| BcnI | CCSGG | 2 cut(s) | 452, 453 |
| BcoDI | GTCTC | 2 cut(s) | 461, 1458 |
| BcuI | ACTAGT | 1 cut(s) | 353 |
| BfaI | CTAG | 4 cut(s) | 155, 188, 342, 354 |
| BfmI | CTRYAG | 1 cut(s) | 294 |
| BfuAI | ACCTGC | 3 cut(s) | 293, 443, 1358 |
| BmcAI | AGTACT | 1 cut(s) | 346 |
| Bme1390I | CCNGG | 5 cut(s) | 412, 452, 453, 1019, 1480 |
| Bme18I | GGWCC | 1 cut(s) | 508 |
| BmeT110I | CYCGRG | 1 cut(s) | 451 |
| BmgBI | CACGTC | 1 cut(s) | 982 |
| BmgT120I | GGNCC | 4 cut(s) | 380, 508, 1391, 1476 |
| BmiI | GGNNCC | 6 cut(s) | 101, 509, 561, 613, 1175, 1393 |
| BmrFI | CCNGG | 5 cut(s) | 412, 452, 453, 1019, 1480 |
| BmrI | ACTGGG | 2 cut(s) | 205, 1172 |
| BmsI | GCATC | 3 cut(s) | 394, 652, 708 |
| BmuI | ACTGGG | 2 cut(s) | 205, 1172 |
| BpiI | GAAGAC | 1 cut(s) | 46 |
| BpmI | CTGGAG | 3 cut(s) | 86, 605, 613 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1095 |
| BpuEI | CTTGAG | 1 cut(s) | 1070 |
| BpuMI | CCSGG | 2 cut(s) | 452, 453 |
| BsaBI | GATNNNNATC | 1 cut(s) | 57 |
| BsaHI | GRCGYC | 1 cut(s) | 388 |
| BsaI | GGTCTC | 2 cut(s) | 461, 1458 |
| BsaJI | CCNNGG | 7 cut(s) | 6, 360, 451, 541, 1354, 1437, 1479 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 99, 129 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 451, 982, 1400, 1474 |
| Bse118I | RCCGGY | 1 cut(s) | 377 |
| Bse1I | ACTGG | 7 cut(s) | 211, 599, 784, 1178, 1338, 1381, 1428 |
| Bse8I | GATNNNNATC | 1 cut(s) | 57 |
| BseBI | CCWGG | 3 cut(s) | 412, 1019, 1480 |
| BseDI | CCNNGG | 7 cut(s) | 6, 360, 451, 541, 1354, 1437, 1479 |
| BseGI | GGATG | 2 cut(s) | 666, 1168 |
| BseJI | GATNNNNATC | 1 cut(s) | 57 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 451, 982, 1400, 1474 |
| BseMII | CTCAG | 2 cut(s) | 959, 1086 |
| BseNI | ACTGG | 7 cut(s) | 211, 599, 784, 1178, 1338, 1381, 1428 |
| BseYI | CCCAGC | 1 cut(s) | 22 |
| BsgI | GTGCAG | 2 cut(s) | 918, 1312 |
| Bsh1236I | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1299 |
| BshFI | GGCC | 6 cut(s) | 5, 381, 444, 489, 1392, 1478 |
| BshNI | GGYRCC | 1 cut(s) | 611 |
| BsiEI | CGRYCG | 1 cut(s) | 1299 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1242, 1254 |
| BsiHKCI | CYCGRG | 1 cut(s) | 451 |
| BsiSI | CCGG | 2 cut(s) | 378, 452 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 451, 982, 1400, 1474 |
| BsmAI | GTCTC | 2 cut(s) | 461, 1458 |
| BsnI | GGCC | 6 cut(s) | 5, 381, 444, 489, 1392, 1478 |
| Bso31I | GGTCTC | 2 cut(s) | 461, 1458 |
| BsoBI | CYCGRG | 1 cut(s) | 451 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1242, 1254 |
| Bsp1407I | TGTACA | 1 cut(s) | 693 |
| BspANI | GGCC | 6 cut(s) | 5, 381, 444, 489, 1392, 1478 |
| BspCNI | CTCAG | 2 cut(s) | 960, 1087 |
| BspFNI | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| BspHI | TCATGA | 1 cut(s) | 742 |
| BspLI | GGNNCC | 6 cut(s) | 101, 509, 561, 613, 1175, 1393 |
| BspMAI | CTGCAG | 1 cut(s) | 298 |
| BspMI | ACCTGC | 3 cut(s) | 293, 443, 1358 |
| BspPI | GGATC | 5 cut(s) | 148, 422, 504, 1119, 1268 |
| BspQI | GCTCTTC | 1 cut(s) | 1259 |
| BspT107I | GGYRCC | 1 cut(s) | 611 |
| BspTNI | GGTCTC | 2 cut(s) | 461, 1458 |
| BsrFI | RCCGGY | 1 cut(s) | 377 |
| BsrGI | TGTACA | 1 cut(s) | 693 |
| BsrI | ACTGG | 7 cut(s) | 211, 599, 784, 1178, 1338, 1381, 1428 |
| BssAI | RCCGGY | 1 cut(s) | 377 |
| BssECI | CCNNGG | 7 cut(s) | 6, 360, 451, 541, 1354, 1437, 1479 |
| BssNI | GRCGYC | 1 cut(s) | 388 |
| BssSI | CACGAG | 1 cut(s) | 1459 |
| BssT1I | CCWWGG | 2 cut(s) | 360, 1437 |
| Bst2BI | CACGAG | 1 cut(s) | 1459 |
| Bst2UI | CCWGG | 3 cut(s) | 412, 1019, 1480 |
| Bst4CI | ACNGT | 4 cut(s) | 466, 542, 796, 1261 |
| Bst6I | CTCTTC | 3 cut(s) | 1226, 1259, 1422 |
| BstACI | GRCGYC | 1 cut(s) | 388 |
| BstAUI | TGTACA | 1 cut(s) | 693 |
| BstC8I | GCNNGC | 3 cut(s) | 379, 535, 864 |
| BstDEI | CTNAG | 2 cut(s) | 968, 1095 |
| BstDSI | CCRYGG | 3 cut(s) | 6, 541, 1354 |
| BstEII | GGTNACC | 1 cut(s) | 1492 |
| BstF5I | GGATG | 2 cut(s) | 666, 1168 |
| BstFNI | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| BstHHI | GCGC | 6 cut(s) | 13, 377, 723, 759, 875, 1295 |
| BstMAI | GTCTC | 2 cut(s) | 461, 1458 |
| BstMCI | CGRYCG | 1 cut(s) | 1299 |
| BstNI | CCWGG | 3 cut(s) | 412, 1019, 1480 |
| BstNSI | RCATGY | 1 cut(s) | 962 |
| BstPI | GGTNACC | 1 cut(s) | 1492 |
| BstSCI | CCNGG | 5 cut(s) | 410, 450, 451, 1017, 1478 |
| BstSFI | CTRYAG | 1 cut(s) | 294 |
| BstUI | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| BstV2I | GAAGAC | 1 cut(s) | 46 |
| BstX2I | RGATCY | 2 cut(s) | 496, 1273 |
| BstYI | RGATCY | 2 cut(s) | 496, 1273 |
| BsuRI | GGCC | 6 cut(s) | 5, 381, 444, 489, 1392, 1478 |
| BtgI | CCRYGG | 3 cut(s) | 6, 541, 1354 |
| BtrI | CACGTC | 1 cut(s) | 982 |
| BtsCI | GGATG | 2 cut(s) | 666, 1168 |
| BtsI | GCAGTG | 3 cut(s) | 906, 1086, 1205 |
| BtsIMutI | CAGTG | 8 cut(s) | 906, 965, 1086, 1185, 1205, 1257, 1331, 1421 |
| BveI | ACCTGC | 3 cut(s) | 293, 443, 1358 |
| Cac8I | GCNNGC | 3 cut(s) | 379, 535, 864 |
| CciI | TCATGA | 1 cut(s) | 742 |
| CfoI | GCGC | 6 cut(s) | 13, 377, 723, 759, 875, 1295 |
| Cfr10I | RCCGGY | 1 cut(s) | 377 |
| Cfr13I | GGNCC | 4 cut(s) | 380, 508, 1391, 1476 |
| Cfr9I | CCCGGG | 1 cut(s) | 451 |
| CseI | GACGC | 2 cut(s) | 396, 396 |
| Csp6I | GTAC | 5 cut(s) | 345, 694, 939, 1267, 1324 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 777, 812 |
| CviAII | CATG | 7 cut(s) | 44, 743, 881, 959, 1145, 1285, 1363 |
| CviQI | GTAC | 5 cut(s) | 345, 694, 939, 1267, 1324 |
| DdeI | CTNAG | 2 cut(s) | 968, 1095 |
| DraIII | CACNNNGTG | 1 cut(s) | 1081 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 3 cut(s) | 1226, 1259, 1422 |
| EarI | CTCTTC | 3 cut(s) | 1226, 1259, 1422 |
| EciI | GGCGGA | 2 cut(s) | 242, 905 |
| Ecl136II | GAGCTC | 1 cut(s) | 1252 |
| Eco130I | CCWWGG | 2 cut(s) | 360, 1437 |
| Eco24I | GRGCYC | 1 cut(s) | 1254 |
| Eco31I | GGTCTC | 2 cut(s) | 461, 1458 |
| Eco47I | GGWCC | 1 cut(s) | 508 |
| Eco53kI | GAGCTC | 1 cut(s) | 1252 |
| Eco88I | CYCGRG | 1 cut(s) | 451 |
| Eco91I | GGTNACC | 1 cut(s) | 1492 |
| EcoICRI | GAGCTC | 1 cut(s) | 1252 |
| EcoO65I | GGTNACC | 1 cut(s) | 1492 |
| EcoRI | GAATTC | 1 cut(s) | 783 |
| EcoRII | CCWGG | 3 cut(s) | 410, 1017, 1478 |
| EcoT14I | CCWWGG | 2 cut(s) | 360, 1437 |
| EcoT38I | GRGCYC | 1 cut(s) | 1254 |
| ErhI | CCWWGG | 2 cut(s) | 360, 1437 |
| FaeI | CATG | 7 cut(s) | 47, 746, 884, 962, 1148, 1288, 1366 |
| FatI | CATG | 7 cut(s) | 43, 742, 880, 958, 1144, 1284, 1362 |
| FauI | CCCGC | 1 cut(s) | 716 |
| FbaI | TGATCA | 2 cut(s) | 124, 1138 |
| FblI | GTMKAC | 1 cut(s) | 1455 |
| FokI | GGATG | 2 cut(s) | 653, 1175 |
| FriOI | GRGCYC | 1 cut(s) | 1254 |
| FspBI | CTAG | 4 cut(s) | 155, 188, 342, 354 |
| GlaI | GCGC | 6 cut(s) | 12, 376, 722, 758, 874, 1294 |
| GsaI | CCCAGC | 1 cut(s) | 26 |
| GsuI | CTGGAG | 3 cut(s) | 86, 605, 613 |
| HaeIII | GGCC | 6 cut(s) | 5, 381, 444, 489, 1392, 1478 |
| HapII | CCGG | 2 cut(s) | 378, 452 |
| HgaI | GACGC | 2 cut(s) | 396, 396 |
| HhaI | GCGC | 6 cut(s) | 13, 377, 723, 759, 875, 1295 |
| Hin1I | GRCGYC | 1 cut(s) | 388 |
| Hin1II | CATG | 7 cut(s) | 47, 746, 884, 962, 1148, 1288, 1366 |
| Hin6I | GCGC | 6 cut(s) | 11, 375, 721, 757, 873, 1293 |
| HinP1I | GCGC | 6 cut(s) | 11, 375, 721, 757, 873, 1293 |
| HincII | GTYRAC | 1 cut(s) | 1456 |
| HindII | GTYRAC | 1 cut(s) | 1456 |
| HindIII | AAGCTT | 1 cut(s) | 581 |
| HinfI | GANTC | 3 cut(s) | 146, 1166, 1359 |
| HpaII | CCGG | 2 cut(s) | 378, 452 |
| HphI | GGTGA | 5 cut(s) | 76, 329, 787, 931, 1486 |
| Hpy166II | GTNNAC | 4 cut(s) | 589, 799, 985, 1456 |
| Hpy188I | TCNGA | 9 cut(s) | 140, 145, 151, 217, 241, 473, 840, 1213, 1278 |
| Hpy188III | TCNNGA | 5 cut(s) | 103, 622, 630, 743, 1001 |
| Hpy8I | GTNNAC | 4 cut(s) | 589, 799, 985, 1456 |
| Hpy99I | CGWCG | 4 cut(s) | 390, 470, 678, 1532 |
| HpyAV | CCTTC | 3 cut(s) | 91, 211, 1016 |
| HpyCH4III | ACNGT | 4 cut(s) | 466, 542, 796, 1261 |
| HpyCH4IV | ACGT | 3 cut(s) | 673, 981, 1191 |
| HpyCH4V | TGCA | 5 cut(s) | 296, 899, 962, 1304, 1329 |
| HpyF3I | CTNAG | 2 cut(s) | 968, 1095 |
| HpySE526I | ACGT | 3 cut(s) | 673, 981, 1191 |
| Hsp92I | GRCGYC | 1 cut(s) | 388 |
| Hsp92II | CATG | 7 cut(s) | 47, 746, 884, 962, 1148, 1288, 1366 |
| HspAI | GCGC | 6 cut(s) | 11, 375, 721, 757, 873, 1293 |
| KroI | GCCGGC | 1 cut(s) | 377 |
| KroNI | GCCGGC | 1 cut(s) | 379 |
| Ksp22I | TGATCA | 2 cut(s) | 124, 1138 |
| LguI | GCTCTTC | 1 cut(s) | 1259 |
| LmnI | GCTCC | 4 cut(s) | 105, 1100, 1213, 1237 |
| LweI | GCATC | 3 cut(s) | 394, 652, 708 |
| MaeI | CTAG | 4 cut(s) | 155, 188, 342, 354 |
| MaeII | ACGT | 3 cut(s) | 673, 981, 1191 |
| MaeIII | GTNAC | 6 cut(s) | 164, 430, 512, 775, 977, 1492 |
| MboII | GAAGA | 8 cut(s) | 51, 68, 196, 332, 638, 1243, 1246, 1439 |
| MflI | RGATCY | 2 cut(s) | 496, 1273 |
| MhlI | GDGCHC | 2 cut(s) | 1242, 1254 |
| MluCI | AATT | 3 cut(s) | 783, 996, 1306 |
| MlyI | GAGTC | 1 cut(s) | 1368 |
| MmeI | TCCRAC | 3 cut(s) | 694, 1191, 1301 |
| MroNI | GCCGGC | 1 cut(s) | 377 |
| MslI | CAYNNNNRTG | 3 cut(s) | 48, 1245, 1334 |
| MspA1I | CMGCKG | 1 cut(s) | 845 |
| MspI | CCGG | 2 cut(s) | 378, 452 |
| MspR9I | CCNGG | 5 cut(s) | 412, 452, 453, 1019, 1480 |
| MvaI | CCWGG | 3 cut(s) | 412, 1019, 1480 |
| MvnI | CGCG | 5 cut(s) | 375, 678, 723, 1293, 1295 |
| NaeI | GCCGGC | 1 cut(s) | 379 |
| NciI | CCSGG | 2 cut(s) | 452, 453 |
| NgoMIV | GCCGGC | 1 cut(s) | 377 |
| NlaIII | CATG | 7 cut(s) | 47, 746, 884, 962, 1148, 1288, 1366 |
| NlaIV | GGNNCC | 6 cut(s) | 101, 509, 561, 613, 1175, 1393 |
| NmuCI | GTSAC | 5 cut(s) | 430, 512, 775, 977, 1492 |
| NspI | RCATGY | 1 cut(s) | 962 |
| OliI | CACNNNNGTG | 1 cut(s) | 1334 |
| PagI | TCATGA | 1 cut(s) | 742 |
| PaqCI | CACCTGC | 1 cut(s) | 293 |
| PciSI | GCTCTTC | 1 cut(s) | 1259 |
| PdiI | GCCGGC | 1 cut(s) | 379 |
| PfeI | GAWTC | 2 cut(s) | 146, 1166 |
| PfoI | TCCNGGA | 1 cut(s) | 410 |
| PleI | GAGTC | 1 cut(s) | 1367 |
| PpsI | GAGTC | 1 cut(s) | 1367 |
| Psp124BI | GAGCTC | 1 cut(s) | 1254 |
| Psp6I | CCWGG | 3 cut(s) | 410, 1017, 1478 |
| PspEI | GGTNACC | 1 cut(s) | 1492 |
| PspFI | CCCAGC | 1 cut(s) | 22 |
| PspGI | CCWGG | 3 cut(s) | 410, 1017, 1478 |
| PspN4I | GGNNCC | 6 cut(s) | 101, 509, 561, 613, 1175, 1393 |
| PspPI | GGNCC | 4 cut(s) | 380, 508, 1391, 1476 |
| PstI | CTGCAG | 1 cut(s) | 298 |
| PsuI | RGATCY | 2 cut(s) | 496, 1273 |
| RsaI | GTAC | 5 cut(s) | 346, 695, 940, 1268, 1325 |
| RsaNI | GTAC | 5 cut(s) | 345, 694, 939, 1267, 1324 |
| RseI | CAYNNNNRTG | 3 cut(s) | 48, 1245, 1334 |
| SacI | GAGCTC | 1 cut(s) | 1254 |
| SalI | GTCGAC | 1 cut(s) | 1454 |
| SapI | GCTCTTC | 1 cut(s) | 1259 |
| Sau96I | GGNCC | 4 cut(s) | 380, 508, 1391, 1476 |
| ScaI | AGTACT | 1 cut(s) | 346 |
| SchI | GAGTC | 1 cut(s) | 1368 |
| ScrFI | CCNGG | 5 cut(s) | 412, 452, 453, 1019, 1480 |
| SduI | GDGCHC | 2 cut(s) | 1242, 1254 |
| SfaNI | GCATC | 3 cut(s) | 394, 652, 708 |
| SfcI | CTRYAG | 1 cut(s) | 294 |
| SinI | GGWCC | 1 cut(s) | 508 |
| SmaI | CCCGGG | 1 cut(s) | 453 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 48, 1245, 1334 |
| SmlI | CTYRAG | 1 cut(s) | 1049 |
| SmoI | CTYRAG | 1 cut(s) | 1049 |
| SpeI | ACTAGT | 1 cut(s) | 353 |
| Sse9I | AATT | 3 cut(s) | 783, 996, 1306 |
| SspI | AATATT | 1 cut(s) | 769 |
| SspMI | CTAG | 4 cut(s) | 155, 188, 342, 354 |
| SstI | GAGCTC | 1 cut(s) | 1254 |
| StyD4I | CCNGG | 5 cut(s) | 410, 450, 451, 1017, 1478 |
| StyI | CCWWGG | 2 cut(s) | 360, 1437 |
| TaaI | ACNGT | 4 cut(s) | 466, 542, 796, 1261 |
| TaiI | ACGT | 3 cut(s) | 676, 984, 1194 |
| TaqI | TCGA | 6 cut(s) | 402, 660, 819, 934, 1122, 1455 |
| TaqII | GACCGA | 1 cut(s) | 1480 |
| TasI | AATT | 3 cut(s) | 783, 996, 1306 |
| TatI | WGTACW | 5 cut(s) | 344, 693, 938, 1266, 1323 |
| TauI | GCSGC | 3 cut(s) | 425, 612, 681 |
| TfiI | GAWTC | 2 cut(s) | 146, 1166 |
| TscAI | CASTG | 8 cut(s) | 906, 972, 1086, 1185, 1205, 1264, 1338, 1428 |
| TseFI | GTSAC | 5 cut(s) | 430, 512, 775, 977, 1492 |
| Tsp45I | GTSAC | 5 cut(s) | 430, 512, 775, 977, 1492 |
| TspDTI | ATGAA | 4 cut(s) | 69, 1179, 1464, 1502 |
| TspGWI | ACGGA | 4 cut(s) | 500, 572, 1371, 1416 |
| TspMI | CCCGGG | 1 cut(s) | 451 |
| TspRI | CASTG | 8 cut(s) | 906, 972, 1086, 1185, 1205, 1264, 1338, 1428 |
| VpaK11BI | GGWCC | 1 cut(s) | 508 |
| XapI | RAATTY | 2 cut(s) | 783, 1306 |
| XceI | RCATGY | 1 cut(s) | 962 |
| XmaI | CCCGGG | 1 cut(s) | 451 |
| XmiI | GTMKAC | 1 cut(s) | 1455 |
| XspI | CTAG | 4 cut(s) | 155, 188, 342, 354 |
| ZrmI | AGTACT | 1 cut(s) | 346 |