Rh5DG002000

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014539
Physical Location & Seq
Forward (+)
180707 .. 182535
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG002000.1

Sequence Viewer

Length: 1380 bp
ATGGCAGAACCCACAACAGAGGAGATGATCTTGTCACTGTCTCCGGTGAAGCTCCACAAACTACCCCACCTGACCGACTATCTCTCACAACTCCCCACCCACACCAACCCACTCCAACACAACCCCTTCTACCACCCTTCCGACGGCTTCTACATAACCCAATCCGATGTCATCCTCCGCCAGATTGTCTACGACCTCGCCGCCGCCTCCGCTCATCCCGCCTCAGCTTACCATCGCGCCGGCCCGCGCCGGCACATCTTGTTTGAGCCGTCGAGCATCAGGGCGGCTATTGTGACATGCGGTGGGCTGTGCCCGGGCATGAACACGGTGGTGAGAGAGCTGGTTCTTGCCCTCTGGGACCTCTACGGAGTCCGTCAGATTTTTGGTGTCAGGGCTGGCTATCGTGGATTTTACTCTATGGATCCCATCCAGCTTAACCCAAAGCTCGTCAACCACTGGCACAAGATTGGCGGCACTGTCCTCGAGACCTCCAGAGGTGGTTTTCATCTTCAAAAGATTGTCGATGCCATCCAACATCGCGGCTATAATCAGGTGTACATTATAGGTGGAGATGGCACAATGCGGGGGGCAGTTGAGATATTTCATGAGATTTGTAGGCGAAAACTGAACATTGCGGTTACTGGGATTCCAAAAACAGTTGACAATGATGTTGGCATCATTGACAGATCTTTCGGATTCCAGACAGCAGTAGAAATGGCCCAGCAGGCAATCAGTGCAGCTCATGTGGAGGCTGAAAGTGCAGTGAATGGAATAGGCTTGGTCAAGTTGATGGGTCGAAGTACAGGCCACATTGCTCTCTATGCAACGTTGAGCAGCCGAGACGTGGACTGCTGCCTAATCCCAGAAACTGAGTTTTACCTGGAAGGAAAAGGAGGGCTATTTGAATTCCTTGAGCAGCGACTGAGGGAGAATGGACACGCAGTGTTAGTTGTGGCTGAAGGAGCGGGACAGGACATAATTCCAAGGAACGAGGCTCAGATACAGGAGAGGGATGAATCTGGAAACCCAGTGTTCCTAGATGTTGGCCAATGGTTGAAGTCGGAGCTCAAGAATTGGTGGAGCAGGGATCACCCCAATGAGCTCTTCACAGTCAAGTACATAGATCCTACATACATGATACGGGCCGTCCCTGCAAATGCTACGGATAACTTGTACTGTACACTTCTTGCCCATTCAGCTATTCATGGAGTGATGGCTGGTTACACTGGATTCGTCCCTGGCCCTATCAATGGCAATTATGCTTACATTCCAGTTGAAGAGGTAGCAAAGGCTAAGAATGAGGTCAACACGAGAGACCACCAATGGGCGTGGGTAAGATCTGTCACAAGCCAGCCTGATTTTATGAGGACTATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

459

Amino Acids

51.08

Weight (kDa)

6.2

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 94 - 402 1e-54 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 212, 965
AccI GTMKAC 1 cut(s) 189
AccII CGCG 3 cut(s) 237, 247, 540
AclI AACGTT 1 cut(s) 827
AclWI GGATC 4 cut(s) 416, 429, 1095, 1118
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 1 cut(s) 905
AcuI CTGAAG 1 cut(s) 978
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 5 cut(s) 557, 802, 1118, 1175, 1180
AfiI CCNNNNNNNGG 4 cut(s) 143, 844, 1250, 1324
AgsI TTSAA 4 cut(s) 512, 905, 1057, 1277
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 2 cut(s) 879, 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
AleI CACNNNNGTG 1 cut(s) 329
AluBI AGCT 9 cut(s) 52, 227, 340, 433, 445, 740, 1066, 1102, 1199
AluI AGCT 9 cut(s) 52, 227, 340, 433, 445, 740, 1066, 1102, 1199
Alw21I GWGCWC 2 cut(s) 1068, 1104
Alw26I GTCTC 4 cut(s) 45, 479, 834, 1308
AlwI GGATC 4 cut(s) 416, 429, 1095, 1118
AlwNI CAGNNNCTG 2 cut(s) 869, 922
Ama87I CYCGRG 2 cut(s) 313, 482
AoxI GGCC 6 cut(s) 241, 717, 805, 1045, 1143, 1240
ApeKI GCWGC 4 cut(s) 737, 834, 852, 916
ApoI RAATTY 1 cut(s) 905
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 2 cut(s) 239, 249
AspS9I GGNCC 5 cut(s) 242, 358, 718, 1143, 1241
AsuC2I CCSGG 2 cut(s) 314, 315
AsuHPI GGTGA 3 cut(s) 58, 343, 1082
AvaI CYCGRG 2 cut(s) 313, 482
AvaII GGWCC 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 1047
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 2 cut(s) 1068, 1104
BauI CACGAG 1 cut(s) 1309
Bbv12I GWGCWC 2 cut(s) 1068, 1104
BbvCI CCTCAGC 1 cut(s) 223
BbvI GCAGC 4 cut(s) 749, 839, 846, 928
BccI CCATC 6 cut(s) 240, 434, 536, 566, 784, 1207
BceAI ACGGC 3 cut(s) 160, 253, 1130
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BciT130I CCWGG 2 cut(s) 881, 1239
BcnI CCSGG 2 cut(s) 314, 315
BcoDI GTCTC 4 cut(s) 45, 479, 834, 1308
BfaI CTAG 1 cut(s) 1037
BglI GCCNNNNNGGC 1 cut(s) 725
BglII AGATCT 2 cut(s) 686, 1337
BisI GCNGC 9 cut(s) 201, 204, 285, 472, 541, 738, 835, 853, 917
BlsI GCNGC 9 cut(s) 202, 205, 286, 473, 542, 739, 836, 854, 918
Bme1390I CCNGG 4 cut(s) 314, 315, 881, 1239
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 313, 482
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 5 cut(s) 242, 358, 718, 1143, 1241
BmiI GGNNCC 2 cut(s) 359, 423
BmrFI CCNGG 4 cut(s) 314, 315, 881, 1239
BmrI ACTGGG 2 cut(s) 651, 1022
BmsI GCATC 3 cut(s) 285, 514, 684
BmuI ACTGGG 2 cut(s) 651, 1022
BpmI CTGGAG 1 cut(s) 475
Bpu10I CCTNAGC 1 cut(s) 223
BpuEI CTTGAG 2 cut(s) 932, 1052
BpuMI CCSGG 2 cut(s) 314, 315
BsaI GGTCTC 2 cut(s) 479, 1308
BsaJI CCNNGG 3 cut(s) 313, 983, 1237
BsaWI WCCGGW 1 cut(s) 43
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 844, 1250, 1324
Bse118I RCCGGY 2 cut(s) 239, 249
Bse1I ACTGG 5 cut(s) 461, 646, 1028, 1231, 1271
Bse3DI GCAATG 2 cut(s) 630, 810
BseBI CCWGG 2 cut(s) 881, 1239
BseDI CCNNGG 3 cut(s) 313, 983, 1237
BseGI GGATG 5 cut(s) 171, 214, 426, 528, 1018
BseLI CCNNNNNNNGG 4 cut(s) 143, 844, 1250, 1324
BseMI GCAATG 2 cut(s) 630, 810
BseMII CTCAG 4 cut(s) 237, 861, 914, 1010
BseNI ACTGG 5 cut(s) 461, 646, 1028, 1231, 1271
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 4 cut(s) 749, 839, 846, 928
BseYI CCCAGC 1 cut(s) 720
BsgI GTGCAG 2 cut(s) 756, 780
Bsh1236I CGCG 3 cut(s) 237, 247, 540
BshFI GGCC 6 cut(s) 243, 719, 807, 1047, 1145, 1242
BsiHKAI GWGCWC 2 cut(s) 1068, 1104
BsiHKCI CYCGRG 2 cut(s) 313, 482
BsiSI CCGG 4 cut(s) 44, 240, 250, 314
BslFI GGGAC 4 cut(s) 371, 981, 1133, 1220
BslI CCNNNNNNNGG 4 cut(s) 143, 844, 1250, 1324
BsmAI GTCTC 4 cut(s) 45, 479, 834, 1308
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 4 cut(s) 371, 981, 1133, 1220
BsnI GGCC 6 cut(s) 243, 719, 807, 1047, 1145, 1242
Bso31I GGTCTC 2 cut(s) 479, 1308
BsoBI CYCGRG 2 cut(s) 313, 482
Bsp1286I GDGCHC 3 cut(s) 314, 1068, 1104
Bsp1407I TGTACA 2 cut(s) 555, 1178
Bsp143I GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
BspANI GGCC 6 cut(s) 243, 719, 807, 1047, 1145, 1242
BspCNI CTCAG 4 cut(s) 236, 862, 915, 1009
BspFNI CGCG 3 cut(s) 237, 247, 540
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 359, 423
BspPI GGATC 4 cut(s) 416, 429, 1095, 1118
BspQI GCTCTTC 1 cut(s) 1109
BspTNI GGTCTC 2 cut(s) 479, 1308
BsrBI CCGCTC 2 cut(s) 212, 965
BsrDI GCAATG 2 cut(s) 630, 810
BsrFI RCCGGY 2 cut(s) 239, 249
BsrGI TGTACA 2 cut(s) 555, 1178
BsrI ACTGG 5 cut(s) 461, 646, 1028, 1231, 1271
BssAI RCCGGY 2 cut(s) 239, 249
BssECI CCNNGG 3 cut(s) 313, 983, 1237
BssMI GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
BssSI CACGAG 1 cut(s) 1309
BssT1I CCWWGG 1 cut(s) 983
Bst2BI CACGAG 1 cut(s) 1309
Bst2UI CCWGG 2 cut(s) 881, 1239
Bst4CI ACNGT 6 cut(s) 39, 328, 478, 658, 1111, 1178
Bst6I CTCTTC 2 cut(s) 1109, 1272
BstAPI GCANNNNNTGC 1 cut(s) 734
BstAUI TGTACA 2 cut(s) 555, 1178
BstC8I GCNNGC 6 cut(s) 241, 245, 251, 397, 726, 1352
BstDEI CTNAG 5 cut(s) 223, 870, 923, 996, 1293
BstF5I GGATG 5 cut(s) 171, 214, 426, 528, 1018
BstFNI CGCG 3 cut(s) 237, 247, 540
BstHHI GCGC 2 cut(s) 239, 249
BstKTI GATC 6 cut(s) 30, 424, 689, 1090, 1126, 1340
BstMAI GTCTC 4 cut(s) 45, 479, 834, 1308
BstMBI GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
BstMWI GCNNNNNNNGC 9 cut(s) 209, 218, 725, 734, 758, 821, 962, 1151, 1196
BstNI CCWGG 2 cut(s) 881, 1239
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 4 cut(s) 312, 313, 879, 1237
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 3 cut(s) 237, 247, 540
BstV1I GCAGC 4 cut(s) 749, 839, 846, 928
BstX2I RGATCY 4 cut(s) 421, 686, 1123, 1337
BstYI RGATCY 4 cut(s) 421, 686, 1123, 1337
BsuRI GGCC 6 cut(s) 243, 719, 807, 1047, 1145, 1242
BtgZI GCGATG 2 cut(s) 218, 521
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 5 cut(s) 171, 214, 426, 528, 1018
BtsI GCAGTG 2 cut(s) 768, 948
BtsIMutI CAGTG 8 cut(s) 35, 454, 474, 739, 768, 948, 1035, 1224
Cac8I GCNNGC 6 cut(s) 241, 245, 251, 397, 726, 1352
CaiI CAGNNNCTG 2 cut(s) 869, 922
CciI TCATGA 1 cut(s) 604
CfoI GCGC 2 cut(s) 239, 249
Cfr10I RCCGGY 2 cut(s) 239, 249
Cfr13I GGNCC 5 cut(s) 242, 358, 718, 1143, 1241
Cfr9I CCCGGG 1 cut(s) 313
Csp6I GTAC 5 cut(s) 556, 801, 1117, 1174, 1179
CspCI CAANNNNNGTGG 2 cut(s) 1310, 1345
CviAII CATG 6 cut(s) 297, 319, 605, 743, 1135, 1205
CviQI GTAC 5 cut(s) 556, 801, 1117, 1174, 1179
DdeI CTNAG 5 cut(s) 223, 870, 923, 996, 1293
DpnI GATC 6 cut(s) 29, 423, 688, 1089, 1125, 1339
DpnII GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
DraIII CACNNNGTG 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 2 cut(s) 1109, 1272
EarI CTCTTC 2 cut(s) 1109, 1272
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 1066, 1102
Eco130I CCWWGG 1 cut(s) 983
Eco24I GRGCYC 2 cut(s) 1068, 1104
Eco31I GGTCTC 2 cut(s) 479, 1308
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 1066, 1102
Eco57I CTGAAG 1 cut(s) 978
Eco88I CYCGRG 2 cut(s) 313, 482
EcoICRI GAGCTC 2 cut(s) 1066, 1102
EcoO109I RGGNCCY 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 2 cut(s) 879, 1237
EcoT14I CCWWGG 1 cut(s) 983
EcoT38I GRGCYC 2 cut(s) 1068, 1104
ErhI CCWWGG 1 cut(s) 983
Esp3I CGTCTC 1 cut(s) 834
FaeI CATG 6 cut(s) 300, 322, 608, 746, 1138, 1208
FaqI GGGAC 4 cut(s) 371, 981, 1133, 1220
FatI CATG 6 cut(s) 296, 318, 604, 742, 1134, 1204
FauI CCCGC 4 cut(s) 226, 252, 576, 958
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 9 cut(s) 201, 204, 285, 472, 541, 738, 835, 853, 917
FokI GGATG 5 cut(s) 158, 201, 413, 515, 1025
FriOI GRGCYC 2 cut(s) 1068, 1104
Fsp4HI GCNGC 9 cut(s) 201, 204, 285, 472, 541, 738, 835, 853, 917
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 2 cut(s) 238, 248
GluI GCNGC 9 cut(s) 201, 204, 285, 472, 541, 738, 835, 853, 917
GsaI CCCAGC 1 cut(s) 724
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 6 cut(s) 243, 719, 807, 1047, 1145, 1242
HapII CCGG 4 cut(s) 44, 240, 250, 314
HhaI GCGC 2 cut(s) 239, 249
Hin1II CATG 6 cut(s) 300, 322, 608, 746, 1138, 1208
Hin6I GCGC 2 cut(s) 237, 247
HinP1I GCGC 2 cut(s) 237, 247
HincII GTYRAC 3 cut(s) 451, 661, 1306
HindII GTYRAC 3 cut(s) 451, 661, 1306
HinfI GANTC 5 cut(s) 369, 646, 696, 1016, 1230
HpaII CCGG 4 cut(s) 44, 240, 250, 314
HphI GGTGA 3 cut(s) 58, 343, 1082
Hpy166II GTNNAC 7 cut(s) 190, 451, 556, 661, 847, 1181, 1306
Hpy188I TCNGA 6 cut(s) 142, 166, 378, 695, 999, 1063
Hpy188III TCNNGA 6 cut(s) 484, 492, 605, 700, 1020, 1069
Hpy8I GTNNAC 7 cut(s) 190, 451, 556, 661, 847, 1181, 1306
Hpy99I CGWCG 2 cut(s) 146, 274
HpyAV CCTTC 4 cut(s) 136, 147, 878, 953
HpyCH4III ACNGT 6 cut(s) 39, 328, 478, 658, 1111, 1178
HpyCH4IV ACGT 2 cut(s) 827, 843
HpyCH4V TGCA 4 cut(s) 737, 761, 824, 1154
HpyF10VI GCNNNNNNNGC 9 cut(s) 209, 218, 725, 734, 758, 821, 962, 1151, 1196
HpyF3I CTNAG 5 cut(s) 223, 870, 923, 996, 1293
HpySE526I ACGT 2 cut(s) 827, 843
Hsp92II CATG 6 cut(s) 300, 322, 608, 746, 1138, 1208
HspAI GCGC 2 cut(s) 237, 247
KroI GCCGGC 2 cut(s) 239, 249
KroNI GCCGGC 2 cut(s) 241, 251
Kzo9I GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
LguI GCTCTTC 1 cut(s) 1109
LmnI GCTCC 4 cut(s) 57, 962, 1063, 1080
Lsp1109I GCAGC 4 cut(s) 749, 839, 846, 928
LweI GCATC 3 cut(s) 285, 514, 684
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 2 cut(s) 827, 843
MaeIII GTNAC 5 cut(s) 33, 292, 637, 1220, 1342
MalI GATC 6 cut(s) 29, 423, 688, 1089, 1125, 1339
MbiI CCGCTC 2 cut(s) 212, 965
MboI GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
MboII GAAGA 3 cut(s) 500, 1096, 1289
MflI RGATCY 4 cut(s) 421, 686, 1123, 1337
MhlI GDGCHC 3 cut(s) 314, 1068, 1104
MlsI TGGCCA 1 cut(s) 1047
MluCI AATT 4 cut(s) 905, 978, 1072, 1255
MluNI TGGCCA 1 cut(s) 1047
MlyI GAGTC 1 cut(s) 378
MmeI TCCRAC 4 cut(s) 139, 165, 556, 1041
Mox20I TGGCCA 1 cut(s) 1047
MroNI GCCGGC 2 cut(s) 239, 249
MscI TGGCCA 1 cut(s) 1047
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 2 cut(s) 329, 1095
Msp20I TGGCCA 1 cut(s) 1047
MspI CCGG 4 cut(s) 44, 240, 250, 314
MspR9I CCNGG 4 cut(s) 314, 315, 881, 1239
MvaI CCWGG 2 cut(s) 881, 1239
MvnI CGCG 3 cut(s) 237, 247, 540
MwoI GCNNNNNNNGC 9 cut(s) 209, 218, 725, 734, 758, 821, 962, 1151, 1196
NaeI GCCGGC 2 cut(s) 241, 251
NciI CCSGG 2 cut(s) 314, 315
NdeII GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
NgoMIV GCCGGC 2 cut(s) 239, 249
NlaIII CATG 6 cut(s) 300, 322, 608, 746, 1138, 1208
NlaIV GGNNCC 2 cut(s) 359, 423
NmeAIII GCCGAG 1 cut(s) 863
NmuCI GTSAC 3 cut(s) 33, 292, 1342
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 329
PaeR7I CTCGAG 1 cut(s) 482
PagI TCATGA 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 1109
PdiI GCCGGC 2 cut(s) 241, 251
PfeI GAWTC 4 cut(s) 646, 696, 1016, 1230
PkrI GCNGC 9 cut(s) 202, 205, 286, 473, 542, 739, 836, 854, 918
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
PpuMI RGGWCCY 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 1068, 1104
Psp1406I AACGTT 1 cut(s) 827
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 879, 1237
PspFI CCCAGC 1 cut(s) 720
PspGI CCWGG 2 cut(s) 879, 1237
PspN4I GGNNCC 2 cut(s) 359, 423
PspPI GGNCC 5 cut(s) 242, 358, 718, 1143, 1241
PspPPI RGGWCCY 1 cut(s) 358
PstNI CAGNNNCTG 2 cut(s) 869, 922
PsuI RGATCY 4 cut(s) 421, 686, 1123, 1337
RsaI GTAC 5 cut(s) 557, 802, 1118, 1175, 1180
RsaNI GTAC 5 cut(s) 556, 801, 1117, 1174, 1179
RseI CAYNNNNRTG 2 cut(s) 329, 1095
SacI GAGCTC 2 cut(s) 1068, 1104
SapI GCTCTTC 1 cut(s) 1109
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 9 cut(s) 201, 204, 285, 472, 541, 738, 835, 853, 917
Sau3AI GATC 6 cut(s) 27, 421, 686, 1087, 1123, 1337
Sau96I GGNCC 5 cut(s) 242, 358, 718, 1143, 1241
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 4 cut(s) 314, 315, 881, 1239
SduI GDGCHC 3 cut(s) 314, 1068, 1104
SfaNI GCATC 3 cut(s) 285, 514, 684
Sfr274I CTCGAG 1 cut(s) 482
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 1 cut(s) 482
SmaI CCCGGG 1 cut(s) 315
SmiMI CAYNNNNRTG 2 cut(s) 329, 1095
SmlI CTYRAG 3 cut(s) 482, 911, 1067
SmoI CTYRAG 3 cut(s) 482, 911, 1067
SrfI GCCCGGGC 1 cut(s) 315
Sse9I AATT 4 cut(s) 905, 978, 1072, 1255
SspMI CTAG 1 cut(s) 1037
SstI GAGCTC 2 cut(s) 1068, 1104
StyD4I CCNGG 4 cut(s) 312, 313, 879, 1237
StyI CCWWGG 1 cut(s) 983
TaaI ACNGT 6 cut(s) 39, 328, 478, 658, 1111, 1178
TaiI ACGT 2 cut(s) 830, 846
TaqI TCGA 4 cut(s) 272, 483, 522, 796
TaqII GACCGA 1 cut(s) 89
TasI AATT 4 cut(s) 905, 978, 1072, 1255
TatI WGTACW 5 cut(s) 555, 800, 1116, 1173, 1178
TauI GCSGC 5 cut(s) 203, 206, 287, 474, 543
TfiI GAWTC 4 cut(s) 646, 696, 1016, 1230
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 8 cut(s) 42, 461, 481, 739, 768, 948, 1035, 1231
TseFI GTSAC 3 cut(s) 33, 292, 1342
TseI GCWGC 4 cut(s) 737, 834, 852, 916
Tsp45I GTSAC 3 cut(s) 33, 292, 1342
TspDTI ATGAA 5 cut(s) 335, 494, 593, 1029, 1193
TspGWI ACGGA 3 cut(s) 362, 381, 1178
TspMI CCCGGG 1 cut(s) 313
TspRI CASTG 8 cut(s) 42, 461, 481, 739, 768, 948, 1035, 1231
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 905
XceI RCATGY 1 cut(s) 300
XhoI CTCGAG 1 cut(s) 482
XmaI CCCGGG 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.