Rw5G000220

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014539
Physical Location & Seq
Forward (+)
215989 .. 217807
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000220.1

Sequence Viewer

Length: 1287 bp
ATGGCAGAACCCACAACAGAGGAGATGATCTTGTCATTGTCTCCAGTGAAGCTCCACAAACTACCCCACCTGACCGACTATCTCTCACAACTCCCCACCCACACCAACCCACTCCAACACAACCCCTTCTACCACCCATCCGACGGCTTCTACATAACCCCATCCGATGTCATCCTCCCTTACCATCGCGCCGGCCCACGACGGCACATCTTGTTTGAGCCGTCGAGCATCAGGGCGGCTATTGTGACATGCGGTGGGCTGTGCCCGGGCATGAACACGGTGTTGAGAGAGCTGGTTCTTGGCCTCTGGGACCTCTACGGAGTCCGTCAGATTTTTGGGATCAAGGCTGGCTATCGTGGATTTTACTCTATGGATCCCATCCCGCTTAACCCAAAGCTCGTCGACCACTGGCACAAGAATGGCGGCACTGTCCTTGAGACCTCCAGAGGTGGTTTTCATCTTCAAAACATTGTCGATGCCATCCAACATCGCGGCTATAATCAGGTGTACATTATAGGTGGAGATGGCACAATGCGGGGGGCAGTTGAGATATTTCATGAGATTTGTAAGCGAAAACTGCACATTGCGGTTACTGGGATTCCAAAAACAGTTGACAATGATGTTGGCATCATTGACAGATCTTTCGGATTCCAGACAGCAGTAGAAATGGCCCAGCAGGCAATCAGTGCAGCTCATGTGGAGGCTGAAAGTGCAGTGAATGGAATAGGCTTGGTCAAGTTGATGGGTCGAAGTACAGGCCACATTGCTCTCCATGCAACATTGAGCAGCCGAGACGTGGACTGCTGCCTAATCCCAGAAACTGAGTTTTACCTGGAAGGAAAAGGAGGGCTTTTTGAATTCCTTGAGCAGCGACTGAGGGAGAATGGACATGCAGTGTTAGTTGTGGCTGAAGGAGCGGGACAGGACATAATTCCAAGGAACGAGGCTCAGAAACAGGAGAGGGATGAATCTGGGAACCCGGTGTTCCTAGATGTTGGCCAATGGTTGAATGAGCTCTTCACAGTCAAGTACATAGATCCTACATACATGATACGGGCCGTCCCTGCAAATGCTACTGATAACTTGTACTGTACACTTCTTGCCCATTCAGCCATTCATGGAGTGATGGCTGGTTACACTGGATTCGTCCCTGGCCCTGTCAATGGCAACTATGCTTACATTCCAGTTGAAGAGGTAGCAAAGGCTAAGAATGAGGTCAACACGAGAGACCACCAATGGGCGTGGGTAAGGTCCGTCACAAACCAGCCTGATTTTATGAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

428

Amino Acids

47.47

Weight (kDa)

6.12

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 78 - 373 2.6e-54 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 917
AccI GTMKAC 1 cut(s) 402
AccII CGCG 2 cut(s) 189, 492
AciI CCGC 8 cut(s) 236, 252, 383, 423, 492, 535, 587, 917
AclWI GGATC 4 cut(s) 347, 368, 381, 1031
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 857
AcuI CTGAAG 1 cut(s) 930
AfaI GTAC 5 cut(s) 509, 754, 1031, 1088, 1093
AfiI CCNNNNNNNGG 4 cut(s) 143, 796, 1163, 1237
AgsI TTSAA 4 cut(s) 464, 857, 1009, 1190
AjiI CACGTC 1 cut(s) 796
AjnI CCWGG 2 cut(s) 831, 1150
AluBI AGCT 5 cut(s) 52, 292, 397, 692, 1015
AluI AGCT 5 cut(s) 52, 292, 397, 692, 1015
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 4 cut(s) 45, 431, 786, 1221
AlwI GGATC 4 cut(s) 347, 368, 381, 1031
AlwNI CAGNNNCTG 2 cut(s) 821, 874
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 7 cut(s) 193, 301, 669, 757, 997, 1056, 1153
ApeKI GCWGC 4 cut(s) 689, 786, 804, 868
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 6 cut(s) 194, 310, 670, 1056, 1154, 1251
AsuC2I CCSGG 3 cut(s) 266, 267, 980
AvaI CYCGRG 1 cut(s) 265
AvaII GGWCC 2 cut(s) 310, 1251
BaeGI GKGCMC 1 cut(s) 266
BalI TGGCCA 1 cut(s) 999
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 1017
BauI CACGAG 1 cut(s) 1222
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 4 cut(s) 701, 791, 798, 880
BccI CCATC 8 cut(s) 145, 169, 192, 386, 488, 518, 736, 1120
BceAI ACGGC 4 cut(s) 160, 205, 218, 1043
BciT130I CCWGG 2 cut(s) 833, 1152
BcnI CCSGG 3 cut(s) 266, 267, 980
BcoDI GTCTC 4 cut(s) 45, 431, 786, 1221
BfaI CTAG 1 cut(s) 989
BglI GCCNNNNNGGC 1 cut(s) 677
BglII AGATCT 1 cut(s) 638
BisI GCNGC 7 cut(s) 237, 424, 493, 690, 787, 805, 869
BlsI GCNGC 7 cut(s) 238, 425, 494, 691, 788, 806, 870
Bme1390I CCNGG 5 cut(s) 266, 267, 833, 980, 1152
Bme18I GGWCC 2 cut(s) 310, 1251
BmeT110I CYCGRG 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 796
BmgT120I GGNCC 6 cut(s) 194, 310, 670, 1056, 1154, 1251
BmiI GGNNCC 3 cut(s) 311, 375, 977
BmrFI CCNGG 5 cut(s) 266, 267, 833, 980, 1152
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 3 cut(s) 237, 466, 636
BmuI ACTGGG 1 cut(s) 603
BpmI CTGGAG 2 cut(s) 27, 427
BpuEI CTTGAG 2 cut(s) 455, 884
BpuMI CCSGG 3 cut(s) 266, 267, 980
BsaI GGTCTC 2 cut(s) 431, 1221
BsaJI CCNNGG 3 cut(s) 265, 935, 1150
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 796, 1163, 1237
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 5 cut(s) 44, 413, 598, 1144, 1184
Bse3DI GCAATG 2 cut(s) 582, 762
BseBI CCWGG 2 cut(s) 833, 1152
BseDI CCNNGG 3 cut(s) 265, 935, 1150
BseGI GGATG 6 cut(s) 137, 161, 171, 378, 480, 970
BseLI CCNNNNNNNGG 4 cut(s) 143, 796, 1163, 1237
BseMI GCAATG 2 cut(s) 582, 762
BseMII CTCAG 3 cut(s) 813, 866, 962
BseNI ACTGG 5 cut(s) 44, 413, 598, 1144, 1184
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 4 cut(s) 701, 791, 798, 880
BseYI CCCAGC 1 cut(s) 672
BsgI GTGCAG 3 cut(s) 563, 708, 732
Bsh1236I CGCG 2 cut(s) 189, 492
BshFI GGCC 7 cut(s) 195, 303, 671, 759, 999, 1058, 1155
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 3 cut(s) 192, 266, 980
BslFI GGGAC 4 cut(s) 323, 933, 1046, 1133
BslI CCNNNNNNNGG 4 cut(s) 143, 796, 1163, 1237
BsmAI GTCTC 4 cut(s) 45, 431, 786, 1221
BsmBI CGTCTC 1 cut(s) 786
BsmFI GGGAC 4 cut(s) 323, 933, 1046, 1133
BsnI GGCC 7 cut(s) 195, 303, 671, 759, 999, 1058, 1155
Bso31I GGTCTC 2 cut(s) 431, 1221
BsoBI CYCGRG 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 266, 1017
Bsp1407I TGTACA 2 cut(s) 507, 1091
Bsp143I GATC 5 cut(s) 27, 339, 373, 638, 1036
BspACI CCGC 8 cut(s) 236, 252, 383, 423, 492, 535, 587, 917
BspANI GGCC 7 cut(s) 195, 303, 671, 759, 999, 1058, 1155
BspCNI CTCAG 3 cut(s) 814, 867, 961
BspFNI CGCG 2 cut(s) 189, 492
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 3 cut(s) 311, 375, 977
BspPI GGATC 4 cut(s) 347, 368, 381, 1031
BspQI GCTCTTC 1 cut(s) 1022
BspTNI GGTCTC 2 cut(s) 431, 1221
BsrBI CCGCTC 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 582, 762
BsrFI RCCGGY 1 cut(s) 191
BsrGI TGTACA 2 cut(s) 507, 1091
BsrI ACTGG 5 cut(s) 44, 413, 598, 1144, 1184
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 3 cut(s) 265, 935, 1150
BssMI GATC 5 cut(s) 27, 339, 373, 638, 1036
BssSI CACGAG 1 cut(s) 1222
BssT1I CCWWGG 1 cut(s) 935
Bst2BI CACGAG 1 cut(s) 1222
Bst2UI CCWGG 2 cut(s) 833, 1152
Bst4CI ACNGT 5 cut(s) 280, 430, 610, 1024, 1091
Bst6I CTCTTC 2 cut(s) 1022, 1185
BstAPI GCANNNNNTGC 1 cut(s) 686
BstAUI TGTACA 2 cut(s) 507, 1091
BstC8I GCNNGC 3 cut(s) 193, 349, 678
BstDEI CTNAG 4 cut(s) 822, 875, 948, 1206
BstF5I GGATG 6 cut(s) 137, 161, 171, 378, 480, 970
BstFNI CGCG 2 cut(s) 189, 492
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 5 cut(s) 30, 342, 376, 641, 1039
BstMAI GTCTC 4 cut(s) 45, 431, 786, 1221
BstMBI GATC 5 cut(s) 27, 339, 373, 638, 1036
BstMWI GCNNNNNNNGC 8 cut(s) 577, 677, 686, 710, 773, 914, 1064, 1109
BstNI CCWGG 2 cut(s) 833, 1152
BstNSI RCATGY 2 cut(s) 252, 893
BstSCI CCNGG 5 cut(s) 264, 265, 831, 978, 1150
BstSLI GKGCMC 1 cut(s) 266
BstUI CGCG 2 cut(s) 189, 492
BstV1I GCAGC 4 cut(s) 701, 791, 798, 880
BstX2I RGATCY 3 cut(s) 373, 638, 1036
BstYI RGATCY 3 cut(s) 373, 638, 1036
BsuRI GGCC 7 cut(s) 195, 303, 671, 759, 999, 1058, 1155
BtgZI GCGATG 2 cut(s) 170, 473
BtrI CACGTC 1 cut(s) 796
BtsCI GGATG 6 cut(s) 137, 161, 171, 378, 480, 970
BtsI GCAGTG 2 cut(s) 720, 900
BtsIMutI CAGTG 7 cut(s) 51, 406, 426, 691, 720, 900, 1137
Cac8I GCNNGC 3 cut(s) 193, 349, 678
CaiI CAGNNNCTG 2 cut(s) 821, 874
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 6 cut(s) 194, 310, 670, 1056, 1154, 1251
Cfr9I CCCGGG 1 cut(s) 265
Csp6I GTAC 5 cut(s) 508, 753, 1030, 1087, 1092
CspCI CAANNNNNGTGG 2 cut(s) 1223, 1258
CviAII CATG 8 cut(s) 249, 271, 557, 695, 773, 890, 1048, 1118
CviQI GTAC 5 cut(s) 508, 753, 1030, 1087, 1092
DdeI CTNAG 4 cut(s) 822, 875, 948, 1206
DpnI GATC 5 cut(s) 29, 341, 375, 640, 1038
DpnII GATC 5 cut(s) 27, 339, 373, 638, 1036
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 2 cut(s) 1022, 1185
EarI CTCTTC 2 cut(s) 1022, 1185
Ecl136II GAGCTC 1 cut(s) 1015
Eco130I CCWWGG 1 cut(s) 935
Eco24I GRGCYC 1 cut(s) 1017
Eco31I GGTCTC 2 cut(s) 431, 1221
Eco47I GGWCC 2 cut(s) 310, 1251
Eco53kI GAGCTC 1 cut(s) 1015
Eco57I CTGAAG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 1015
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 831, 1150
EcoT14I CCWWGG 1 cut(s) 935
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 935
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 8 cut(s) 252, 274, 560, 698, 776, 893, 1051, 1121
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FaqI GGGAC 4 cut(s) 323, 933, 1046, 1133
FatI CATG 8 cut(s) 248, 270, 556, 694, 772, 889, 1047, 1117
FauI CCCGC 3 cut(s) 390, 528, 910
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 7 cut(s) 237, 424, 493, 690, 787, 805, 869
FokI GGATG 6 cut(s) 124, 148, 158, 365, 467, 977
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 7 cut(s) 237, 424, 493, 690, 787, 805, 869
FspBI CTAG 1 cut(s) 989
GlaI GCGC 1 cut(s) 190
GluI GCNGC 7 cut(s) 237, 424, 493, 690, 787, 805, 869
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 2 cut(s) 27, 427
HaeIII GGCC 7 cut(s) 195, 303, 671, 759, 999, 1058, 1155
HapII CCGG 3 cut(s) 192, 266, 980
HhaI GCGC 1 cut(s) 191
Hin1II CATG 8 cut(s) 252, 274, 560, 698, 776, 893, 1051, 1121
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 3 cut(s) 403, 613, 1219
HindII GTYRAC 3 cut(s) 403, 613, 1219
HinfI GANTC 5 cut(s) 321, 598, 648, 968, 1143
HpaII CCGG 3 cut(s) 192, 266, 980
Hpy166II GTNNAC 6 cut(s) 403, 508, 613, 799, 1094, 1219
Hpy188I TCNGA 5 cut(s) 142, 166, 330, 647, 951
Hpy188III TCNNGA 3 cut(s) 444, 557, 652
Hpy8I GTNNAC 6 cut(s) 403, 508, 613, 799, 1094, 1219
Hpy99I CGWCG 4 cut(s) 146, 204, 226, 404
HpyAV CCTTC 3 cut(s) 136, 830, 905
HpyCH4III ACNGT 5 cut(s) 280, 430, 610, 1024, 1091
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 6 cut(s) 580, 689, 713, 776, 893, 1067
HpyF10VI GCNNNNNNNGC 8 cut(s) 577, 677, 686, 710, 773, 914, 1064, 1109
HpyF3I CTNAG 4 cut(s) 822, 875, 948, 1206
HpySE526I ACGT 1 cut(s) 795
Hsp92II CATG 8 cut(s) 252, 274, 560, 698, 776, 893, 1051, 1121
HspAI GCGC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Kzo9I GATC 5 cut(s) 27, 339, 373, 638, 1036
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 2 cut(s) 57, 914
Lsp1109I GCAGC 4 cut(s) 701, 791, 798, 880
LweI GCATC 3 cut(s) 237, 466, 636
MaeI CTAG 1 cut(s) 989
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 4 cut(s) 244, 589, 1133, 1255
MalI GATC 5 cut(s) 29, 341, 375, 640, 1038
MbiI CCGCTC 1 cut(s) 917
MboI GATC 5 cut(s) 27, 339, 373, 638, 1036
MboII GAAGA 3 cut(s) 452, 1009, 1202
MflI RGATCY 3 cut(s) 373, 638, 1036
MhlI GDGCHC 2 cut(s) 266, 1017
MlsI TGGCCA 1 cut(s) 999
MluCI AATT 2 cut(s) 857, 930
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 3 cut(s) 139, 165, 508
Mox20I TGGCCA 1 cut(s) 999
MroNI GCCGGC 1 cut(s) 191
MscI TGGCCA 1 cut(s) 999
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 999
MspI CCGG 3 cut(s) 192, 266, 980
MspR9I CCNGG 5 cut(s) 266, 267, 833, 980, 1152
MvaI CCWGG 2 cut(s) 833, 1152
MvnI CGCG 2 cut(s) 189, 492
MwoI GCNNNNNNNGC 8 cut(s) 577, 677, 686, 710, 773, 914, 1064, 1109
NaeI GCCGGC 1 cut(s) 193
NciI CCSGG 3 cut(s) 266, 267, 980
NdeII GATC 5 cut(s) 27, 339, 373, 638, 1036
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 8 cut(s) 252, 274, 560, 698, 776, 893, 1051, 1121
NlaIV GGNNCC 3 cut(s) 311, 375, 977
NmeAIII GCCGAG 1 cut(s) 815
NmuCI GTSAC 2 cut(s) 244, 1255
NspI RCATGY 2 cut(s) 252, 893
PagI TCATGA 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 1022
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 4 cut(s) 598, 648, 968, 1143
PkrI GCNGC 7 cut(s) 238, 425, 494, 691, 788, 806, 870
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 1017
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 831, 1150
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 2 cut(s) 831, 1150
PspN4I GGNNCC 3 cut(s) 311, 375, 977
PspPI GGNCC 6 cut(s) 194, 310, 670, 1056, 1154, 1251
PspPPI RGGWCCY 1 cut(s) 310
PstNI CAGNNNCTG 2 cut(s) 821, 874
PsuI RGATCY 3 cut(s) 373, 638, 1036
RsaI GTAC 5 cut(s) 509, 754, 1031, 1088, 1093
RsaNI GTAC 5 cut(s) 508, 753, 1030, 1087, 1092
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 1017
SalI GTCGAC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 7 cut(s) 237, 424, 493, 690, 787, 805, 869
Sau3AI GATC 5 cut(s) 27, 339, 373, 638, 1036
Sau96I GGNCC 6 cut(s) 194, 310, 670, 1056, 1154, 1251
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 5 cut(s) 266, 267, 833, 980, 1152
SduI GDGCHC 2 cut(s) 266, 1017
SfaNI GCATC 3 cut(s) 237, 466, 636
SinI GGWCC 2 cut(s) 310, 1251
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 2 cut(s) 434, 863
SmoI CTYRAG 2 cut(s) 434, 863
SrfI GCCCGGGC 1 cut(s) 267
Sse9I AATT 2 cut(s) 857, 930
SsiI CCGC 8 cut(s) 236, 252, 383, 423, 492, 535, 587, 917
SspMI CTAG 1 cut(s) 989
SstI GAGCTC 1 cut(s) 1017
StyD4I CCNGG 5 cut(s) 264, 265, 831, 978, 1150
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 5 cut(s) 280, 430, 610, 1024, 1091
TaiI ACGT 1 cut(s) 798
TaqI TCGA 4 cut(s) 224, 402, 474, 748
TaqII GACCGA 1 cut(s) 89
TasI AATT 2 cut(s) 857, 930
TatI WGTACW 5 cut(s) 507, 752, 1029, 1086, 1091
TauI GCSGC 3 cut(s) 239, 426, 495
TfiI GAWTC 4 cut(s) 598, 648, 968, 1143
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 7 cut(s) 51, 413, 433, 691, 720, 900, 1144
TseFI GTSAC 2 cut(s) 244, 1255
TseI GCWGC 4 cut(s) 689, 786, 804, 868
Tsp45I GTSAC 2 cut(s) 244, 1255
TspDTI ATGAA 5 cut(s) 287, 446, 545, 981, 1106
TspGWI ACGGA 3 cut(s) 314, 333, 1243
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 7 cut(s) 51, 413, 433, 691, 720, 900, 1144
VpaK11BI GGWCC 2 cut(s) 310, 1251
XapI RAATTY 1 cut(s) 857
XceI RCATGY 2 cut(s) 252, 893
XmaI CCCGGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.