FvH4_3g00580

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014118
Physical Location & Seq
Forward (+)
295870 .. 297647
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00580.t1

Sequence Viewer

Length: 618 bp
ATGAGCTACTTGGGTGTGGGTGTTAGTCCTGGGAATGTCGCAGTGTCTCACAGCAGTAGCACCACAAATCTAAAAGTGATCGATCGGAGAGTGAAGGTGGCAGAGTTGGTCTTGAGGTTTCTCATCTGTGGTCTCGGACTCGCCGCTGCTCTTCTTGTGGCAACTGACACTCAAGTCAAAGAGATCTTCATGGTCAAGAAGAAGGCCAAGTTCACAGACATGAAGGCTCTAGTGTTTTTGGTGATAGTCAATGGAATAGCTGCTGCCTACTCTTTGCTGCAAGCACTGCGCTGTGTTGTGAGCATGATTAAAGGAATTGTTCTCTTCAGCAAGCCCTTAGCTTGGATGATTTTCTCGGCTGATCAGGTGGTGGCTTATGTGGCTGTGGCCGAAGTAGGAGCTGCAGCACAGTCTGCGGCGTTGGCCAAGTTGGGGCAGTCGGAGCTACAATGGATGAAGATCTGCAACATGTATGACAAGTTCTGCACCCAAGTTGGGGAGGGAATTGCGATCTCGGTCTTGGTTGGTCTCAGCACAATCCTCATCTCTTGCATTTCTGCTTTCAATCTCTTCCGATTGTATGGTGGCGGCAAGAACCAGAGCAGCTCAAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

206

Amino Acids

21.92

Weight (kDa)

9.56

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 174 4.1e-43 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AciI CCGC 3 cut(s) 144, 416, 588
AcoI YGGCCR 2 cut(s) 387, 423
AcuI CTGAAG 1 cut(s) 310
AfiI CCNNNNNNNGG 4 cut(s) 342, 432, 495, 496
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 1 cut(s) 565
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 6 cut(s) 6, 260, 341, 401, 445, 606
AluI AGCT 6 cut(s) 6, 260, 341, 401, 445, 606
Alw26I GTCTC 3 cut(s) 51, 137, 533
AoxI GGCC 3 cut(s) 204, 387, 423
ApeKI GCWGC 7 cut(s) 146, 260, 263, 277, 401, 404, 603
AspLEI GCGC 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 253
BalI TGGCCA 1 cut(s) 425
BbvI GCAGC 6 cut(s) 133, 247, 250, 264, 388, 416
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 51, 137, 533
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 402
BglII AGATCT 2 cut(s) 183, 459
Bme1390I CCNGG 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 337
BpuEI CTTGAG 3 cut(s) 133, 156, 592
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 458
BsaI GGTCTC 2 cut(s) 137, 533
BsaJI CCNNGG 1 cut(s) 29
Bsc4I CCNNNNNNNGG 4 cut(s) 342, 432, 495, 496
Bse8I GATNNNNATC 1 cut(s) 458
BseBI CCWGG 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 2 cut(s) 351, 459
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 4 cut(s) 342, 432, 495, 496
BseMII CTCAG 1 cut(s) 544
BseXI GCAGC 6 cut(s) 133, 247, 250, 264, 388, 416
BsgI GTGCAG 1 cut(s) 469
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 3 cut(s) 206, 389, 425
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 85
BslI CCNNNNNNNGG 4 cut(s) 342, 432, 495, 496
BsmAI GTCTC 3 cut(s) 51, 137, 533
BsnI GGCC 3 cut(s) 206, 389, 425
Bso31I GGTCTC 2 cut(s) 137, 533
Bsp143I GATC 6 cut(s) 78, 82, 183, 361, 459, 510
BspACI CCGC 3 cut(s) 144, 416, 588
BspANI GGCC 3 cut(s) 206, 389, 425
BspCNI CTCAG 1 cut(s) 543
BspDI ATCGAT 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 406
BspQI GCTCTTC 1 cut(s) 156
BspTNI GGTCTC 2 cut(s) 137, 533
BssECI CCNNGG 1 cut(s) 29
BssMI GATC 6 cut(s) 78, 82, 183, 361, 459, 510
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 3 cut(s) 156, 329, 575
BstAPI GCANNNNNTGC 2 cut(s) 286, 413
BstC8I GCNNGC 2 cut(s) 282, 332
BstDEI CTNAG 2 cut(s) 337, 530
BstF5I GGATG 2 cut(s) 351, 459
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 6 cut(s) 81, 85, 186, 364, 462, 513
BstMAI GTCTC 3 cut(s) 51, 137, 533
BstMBI GATC 6 cut(s) 78, 82, 183, 361, 459, 510
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 5 cut(s) 286, 380, 413, 422, 442
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 6 cut(s) 133, 247, 250, 264, 388, 416
BstX2I RGATCY 2 cut(s) 183, 459
BstYI RGATCY 2 cut(s) 183, 459
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 3 cut(s) 206, 389, 425
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 2 cut(s) 351, 459
BtsI GCAGTG 2 cut(s) 48, 284
BtsIMutI CAGTG 2 cut(s) 48, 284
Cac8I GCNNGC 2 cut(s) 282, 332
CfoI GCGC 1 cut(s) 291
ClaI ATCGAT 1 cut(s) 81
CviAII CATG 4 cut(s) 190, 220, 304, 469
DdeI CTNAG 2 cut(s) 337, 530
DpnI GATC 6 cut(s) 80, 84, 185, 363, 461, 512
DpnII GATC 6 cut(s) 78, 82, 183, 361, 459, 510
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 2 cut(s) 387, 423
Eam1104I CTCTTC 3 cut(s) 156, 329, 575
EarI CTCTTC 3 cut(s) 156, 329, 575
Eco31I GGTCTC 2 cut(s) 137, 533
Eco57I CTGAAG 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 28
FaeI CATG 4 cut(s) 193, 223, 307, 472
FaiI YATR 7 cut(s) 191, 221, 305, 378, 470, 474, 582
FatI CATG 4 cut(s) 189, 219, 303, 468
FbaI TGATCA 1 cut(s) 361
FokI GGATG 2 cut(s) 358, 466
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 290
HaeIII GGCC 3 cut(s) 206, 389, 425
HhaI GCGC 1 cut(s) 291
Hin1II CATG 4 cut(s) 193, 223, 307, 472
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 1 cut(s) 138
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 4 cut(s) 87, 137, 442, 575
Hpy188III TCNNGA 2 cut(s) 112, 196
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 3 cut(s) 88, 196, 217
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 280, 404, 465, 486, 552
HpyF10VI GCNNNNNNNGC 5 cut(s) 286, 380, 413, 422, 442
HpyF3I CTNAG 2 cut(s) 337, 530
Hsp92II CATG 4 cut(s) 193, 223, 307, 472
HspAI GCGC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 6 cut(s) 78, 82, 183, 361, 459, 510
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 2 cut(s) 398, 442
LpnPI CCDG 4 cut(s) 15, 42, 350, 611
Lsp1109I GCAGC 6 cut(s) 133, 247, 250, 264, 388, 416
MaeI CTAG 1 cut(s) 230
MalI GATC 6 cut(s) 80, 84, 185, 363, 461, 512
MboI GATC 6 cut(s) 78, 82, 183, 361, 459, 510
MboII GAAGA 6 cut(s) 143, 178, 211, 316, 469, 562
MflI RGATCY 2 cut(s) 183, 459
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 2 cut(s) 315, 504
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 3 cut(s) 108, 493, 551
Mox20I TGGCCA 1 cut(s) 425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 5 cut(s) 286, 380, 413, 422, 442
NdeII GATC 6 cut(s) 78, 82, 183, 361, 459, 510
NlaIII CATG 4 cut(s) 193, 223, 307, 472
NmeAIII GCCGAG 1 cut(s) 335
NspI RCATGY 1 cut(s) 472
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 156
Ple19I CGATCG 1 cut(s) 85
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 2 cut(s) 183, 459
PvuI CGATCG 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 309
Sau3AI GATC 6 cut(s) 78, 82, 183, 361, 459, 510
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 3 cut(s) 112, 171, 607
SmoI CTYRAG 3 cut(s) 112, 171, 607
Sse9I AATT 2 cut(s) 315, 504
SsiI CCGC 3 cut(s) 144, 416, 588
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 1 cut(s) 411
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 505
TasI AATT 2 cut(s) 315, 504
TauI GCSGC 3 cut(s) 146, 419, 591
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 48, 291
TseI GCWGC 7 cut(s) 146, 260, 263, 277, 401, 404, 603
TspDTI ATGAA 3 cut(s) 178, 236, 470
TspRI CASTG 2 cut(s) 48, 291
XceI RCATGY 1 cut(s) 472
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.