MD00G1058300.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014118
Physical Location & Seq
Forward (+)
10638948 .. 10641413
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1058300.v1.1.491

Sequence Viewer

Length: 609 bp
ATGAGCTACTTGGGTGTTGGTGTGAGTCCTGGGAATGTTCCGGTGTATCACGGCACCAACTTAAAAGTAATTGATCGGAGGGTGAGGGTGGCCGAGTTGGTTTTGAGGTGTGTAATCTGCGGTCTAGGAGTTGTTGCTGCTGCTCTTGTAGGGACTGATACTCAGATCAAAGAGATCTTCACTGTCCAGAAGAAAGCTAAATTTACGGACATGAAAGCTCTTGTGTTTTTGGTGGTGGCTAATGGGATAGTTGCTGCCTACTCTTTGGTGCAAGTGATGCGTTGTGTTGTGAGCATGGTTAGAGGCAATGTGCTCTTCAGCAAGCCCATAGCTTGGATCATTTTCTCGGGCGATCAGGTGATGGCATATGTGAGTGTGGCAGCAGTAGGAGCTGCGGCACAGTCGGCGGTGTTTGCGAAGTTGGGGCAGCCAGAGCTGCAATGGATGAAGATATGCAATATGTATGACAAGTTTTGCAACCGAGTTGGGGAGGGAATTGCCAGCGCTTTATTAGTCAGTCTAAGCGCAGTGCTGCTCTCGTGTGTTTCTGCTTTCAATCTCTTCCGCTTGTACGGTGGCAACACGAACAAGACCGCCGCCAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

203

Amino Acids

21.69

Weight (kDa)

9.63

Isoelectric Point (pI)

20.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 26 - 173 2.2e-46 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 333
AciI CCGC 6 cut(s) 120, 395, 407, 565, 594, 597
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 301
AfaI GTAC 1 cut(s) 572
AfeI AGCGCT 1 cut(s) 505
AfiI CCNNNNNNNGG 4 cut(s) 333, 487, 600, 603
AgsI TTSAA 1 cut(s) 556
AjnI CCWGG 2 cut(s) 28, 599
AluBI AGCT 6 cut(s) 6, 197, 218, 332, 392, 436
AluI AGCT 6 cut(s) 6, 197, 218, 332, 392, 436
Alw21I GWGCWC 1 cut(s) 315
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 346
Aor51HI AGCGCT 1 cut(s) 505
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 8 cut(s) 137, 140, 254, 380, 392, 427, 436, 532
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 2 cut(s) 506, 527
AsuHPI GGTGA 2 cut(s) 94, 370
AvaI CYCGRG 1 cut(s) 346
BanI GGYRCC 1 cut(s) 53
BauI CACGAG 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 8 cut(s) 124, 127, 241, 379, 392, 423, 439, 519
BccI CCATC 1 cut(s) 355
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 2 cut(s) 30, 601
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 507
BglII AGATCT 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 30, 601
BmeT110I CYCGRG 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 30, 601
BmsI GCATC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 29
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 487, 600, 603
Bse3DI GCAATG 2 cut(s) 313, 446
BseBI CCWGG 2 cut(s) 30, 601
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 1 cut(s) 450
BseLI CCNNNNNNNGG 4 cut(s) 333, 487, 600, 603
BseMI GCAATG 2 cut(s) 313, 446
BseMII CTCAG 1 cut(s) 176
BseXI GCAGC 8 cut(s) 124, 127, 241, 379, 392, 423, 439, 519
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 346
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 4 cut(s) 333, 487, 600, 603
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 5 cut(s) 73, 165, 174, 336, 352
BspACI CCGC 6 cut(s) 120, 395, 407, 565, 594, 597
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 313, 446
BssECI CCNNGG 1 cut(s) 29
BssMI GATC 5 cut(s) 73, 165, 174, 336, 352
BssSI CACGAG 1 cut(s) 538
Bst2BI CACGAG 1 cut(s) 538
Bst2UI CCWGG 2 cut(s) 30, 601
Bst4CI ACNGT 3 cut(s) 184, 402, 575
Bst6I CTCTTC 2 cut(s) 320, 566
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 323, 502
BstDEI CTNAG 2 cut(s) 162, 521
BstF5I GGATG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 2 cut(s) 506, 527
BstKTI GATC 5 cut(s) 76, 168, 177, 339, 355
BstMBI GATC 5 cut(s) 73, 165, 174, 336, 352
BstMWI GCNNNNNNNGC 6 cut(s) 277, 389, 404, 413, 433, 436
BstNI CCWGG 2 cut(s) 30, 601
BstSCI CCNGG 2 cut(s) 28, 599
BstV1I GCAGC 8 cut(s) 124, 127, 241, 379, 392, 423, 439, 519
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 450
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 180, 534
Cac8I GCNNGC 2 cut(s) 323, 502
CfoI GCGC 2 cut(s) 506, 527
Csp6I GTAC 1 cut(s) 571
CviAII CATG 2 cut(s) 211, 295
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 2 cut(s) 162, 521
DpnI GATC 5 cut(s) 75, 167, 176, 338, 354
DpnII GATC 5 cut(s) 73, 165, 174, 336, 352
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 320, 566
EarI CTCTTC 2 cut(s) 320, 566
Eco47III AGCGCT 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 28, 599
FaeI CATG 2 cut(s) 214, 298
FaiI YATR 8 cut(s) 212, 296, 329, 367, 369, 454, 461, 465
FaqI GGGAC 1 cut(s) 166
FatI CATG 2 cut(s) 210, 294
FauNDI CATATG 1 cut(s) 367
FokI GGATG 1 cut(s) 457
FspBI CTAG 1 cut(s) 125
GlaI GCGC 2 cut(s) 505, 526
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 41
HhaI GCGC 2 cut(s) 506, 527
Hin1II CATG 2 cut(s) 214, 298
Hin6I GCGC 2 cut(s) 504, 525
HinP1I GCGC 2 cut(s) 504, 525
HinfI GANTC 1 cut(s) 25
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 94, 370
Hpy188I TCNGA 2 cut(s) 78, 165
Hpy188III TCNNGA 1 cut(s) 187
HpyCH4III ACNGT 3 cut(s) 184, 402, 575
HpyCH4V TGCA 4 cut(s) 271, 439, 456, 477
HpyF10VI GCNNNNNNNGC 6 cut(s) 277, 389, 404, 413, 433, 436
HpyF3I CTNAG 2 cut(s) 162, 521
Hsp92II CATG 2 cut(s) 214, 298
HspAI GCGC 2 cut(s) 504, 525
Kzo9I GATC 5 cut(s) 73, 165, 174, 336, 352
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 8 cut(s) 15, 42, 54, 200, 341, 444, 514, 586
Lsp1109I GCAGC 8 cut(s) 124, 127, 241, 379, 392, 423, 439, 519
LweI GCATC 1 cut(s) 267
MaeI CTAG 1 cut(s) 125
MalI GATC 5 cut(s) 75, 167, 176, 338, 354
MboI GATC 5 cut(s) 73, 165, 174, 336, 352
MboII GAAGA 5 cut(s) 169, 202, 307, 460, 553
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 3 cut(s) 69, 200, 495
MlyI GAGTC 1 cut(s) 34
MnlI CCTC 5 cut(s) 72, 78, 99, 296, 484
MseI TTAA 1 cut(s) 62
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 2 cut(s) 30, 601
MvaI CCWGG 2 cut(s) 30, 601
MwoI GCNNNNNNNGC 6 cut(s) 277, 389, 404, 413, 433, 436
NdeI CATATG 1 cut(s) 367
NdeII GATC 5 cut(s) 73, 165, 174, 336, 352
NlaIII CATG 2 cut(s) 214, 298
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 333
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 28, 599
PspGI CCWGG 2 cut(s) 28, 599
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 62
Sau3AI GATC 5 cut(s) 73, 165, 174, 336, 352
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 30, 601
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 8, 110, 199, 220, 334, 360, 394, 438, 605
SfaNI GCATC 1 cut(s) 267
Sse9I AATT 3 cut(s) 69, 200, 495
SsiI CCGC 6 cut(s) 120, 395, 407, 565, 594, 597
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 28, 599
TaaI ACNGT 3 cut(s) 184, 402, 575
TasI AATT 3 cut(s) 69, 200, 495
TauI GCSGC 2 cut(s) 398, 599
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 2 cut(s) 187, 534
TseI GCWGC 8 cut(s) 137, 140, 254, 380, 392, 427, 436, 532
TspDTI ATGAA 2 cut(s) 227, 461
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 187, 534
Van91I CCANNNNNTGG 1 cut(s) 333
XapI RAATTY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.