Rw5G000670

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014118
Physical Location & Seq
Forward (+)
455336 .. 456884
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000670.1

Sequence Viewer

Length: 624 bp
ATGAGCTACTTGGGTGTGGGTGTTAGTCCTGGGAATGTCCCAGTGTCTCACAGCAGCAGCAGCAGCACCACAAATCTAAAAGTGATCGATCGGAGAGTGAGGGTGGCAGAGTTGGTCTTGAGGTTTCTCATCTGTGGTCTCGGACTCGCCGCTGCTCTTCTTGTGGCCACTGATACTCAGGTCAAAGAGATCTTCATGGTCAAGAAGAAGGCCAAGTTCACGGACATGAAGGCCCTTGTGTTTTTGGTGATAGCCAATGGAATAGCTGCTGCTTACTCTTTGCTGCAAGCACTGCGCTGTGTTATGAGCATGGTTAGAGGAAATGTTCTCTTCAGCAAGCCCTTAGCTTGGATTATATTCTCAGCTGATCAGGTGATGGCATATGTGGCTGTGGCTGCAATAGGAGCTGCGGCACAGTCTGCGGCCTTGGCCAAGTTGGGGCAGTCGGAGCTACAATGGATGAAGATCTGCAACATGTATGACAAGTTCTGCAACCAAGTTGGGGAGGGAATTGCGAGCGCGGTGTTAGTTGGCCTCAGCACAATCCTCATCTCTTGCATTTCTGCTTTCAATCTCTTCCGATTGTATGGTGGCAACAAGAACAAGAGCAGCTCCAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

207

Amino Acids

22.16

Weight (kDa)

9.77

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 31 - 176 1.8e-46 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 521
AciI CCGC 4 cut(s) 150, 410, 422, 521
AcoI YGGCCR 2 cut(s) 165, 429
AcuI CTGAAG 1 cut(s) 316
AfiI CCNNNNNNNGG 3 cut(s) 348, 438, 502
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 1 cut(s) 571
AjnI CCWGG 2 cut(s) 28, 614
AluBI AGCT 7 cut(s) 6, 266, 347, 365, 407, 451, 612
AluI AGCT 7 cut(s) 6, 266, 347, 365, 407, 451, 612
Alw26I GTCTC 2 cut(s) 51, 143
AoxI GGCC 6 cut(s) 165, 210, 231, 423, 429, 532
AspLEI GCGC 2 cut(s) 297, 521
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 2 cut(s) 259, 385
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BalI TGGCCA 2 cut(s) 167, 431
BbvCI CCTCAGC 1 cut(s) 536
BccI CCATC 1 cut(s) 370
BciT130I CCWGG 2 cut(s) 30, 616
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 51, 143
BglII AGATCT 2 cut(s) 189, 465
Bme1390I CCNGG 2 cut(s) 30, 616
BmgT120I GGNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 30, 616
BmrI ACTGGG 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 598
Bpu10I CCTNAGC 2 cut(s) 343, 536
BpuEI CTTGAG 1 cut(s) 139
Bsa29I ATCGAT 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 464
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 29, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 438, 502
Bse1I ACTGG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 2 cut(s) 30, 616
BseCI ATCGAT 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 29, 426
BseGI GGATG 1 cut(s) 465
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 348, 438, 502
BseMII CTCAG 3 cut(s) 191, 375, 550
BseNI ACTGG 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 6 cut(s) 167, 212, 233, 425, 431, 534
BshVI ATCGAT 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 348, 438, 502
BsmAI GTCTC 2 cut(s) 51, 143
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 6 cut(s) 167, 212, 233, 425, 431, 534
Bso31I GGTCTC 1 cut(s) 143
Bsp143I GATC 5 cut(s) 84, 88, 189, 367, 465
BspACI CCGC 4 cut(s) 150, 410, 422, 521
BspANI GGCC 6 cut(s) 167, 212, 233, 425, 431, 534
BspCNI CTCAG 3 cut(s) 190, 374, 549
BspDI ATCGAT 1 cut(s) 87
BspFNI CGCG 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 162
BspTNI GGTCTC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 29, 426
BssMI GATC 5 cut(s) 84, 88, 189, 367, 465
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 30, 616
Bst4CI ACNGT 1 cut(s) 417
Bst6I CTCTTC 3 cut(s) 162, 335, 581
BstAPI GCANNNNNTGC 2 cut(s) 292, 419
BstC8I GCNNGC 3 cut(s) 288, 338, 517
BstDEI CTNAG 4 cut(s) 177, 343, 361, 536
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 521
BstHHI GCGC 2 cut(s) 297, 521
BstKTI GATC 5 cut(s) 87, 91, 192, 370, 468
BstMAI GTCTC 2 cut(s) 51, 143
BstMBI GATC 5 cut(s) 84, 88, 189, 367, 465
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 9 cut(s) 60, 63, 292, 386, 395, 404, 419, 428, 448
BstNI CCWGG 2 cut(s) 30, 616
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 28, 614
BstUI CGCG 1 cut(s) 521
BstX2I RGATCY 2 cut(s) 189, 465
BstYI RGATCY 2 cut(s) 189, 465
Bsu15I ATCGAT 1 cut(s) 87
BsuRI GGCC 6 cut(s) 167, 212, 233, 425, 431, 534
BsuTUI ATCGAT 1 cut(s) 87
BtsCI GGATG 1 cut(s) 465
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 3 cut(s) 48, 168, 290
Cac8I GCNNGC 3 cut(s) 288, 338, 517
CfoI GCGC 2 cut(s) 297, 521
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 87
CviAII CATG 4 cut(s) 196, 226, 310, 475
DdeI CTNAG 4 cut(s) 177, 343, 361, 536
DpnI GATC 5 cut(s) 86, 90, 191, 369, 467
DpnII GATC 5 cut(s) 84, 88, 189, 367, 465
EaeI YGGCCR 2 cut(s) 165, 429
Eam1104I CTCTTC 3 cut(s) 162, 335, 581
EarI CTCTTC 3 cut(s) 162, 335, 581
Eco130I CCWWGG 1 cut(s) 426
Eco31I GGTCTC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 28, 614
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 4 cut(s) 199, 229, 313, 478
FaqI GGGAC 1 cut(s) 23
FatI CATG 4 cut(s) 195, 225, 309, 474
FauNDI CATATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 367
FokI GGATG 1 cut(s) 472
GlaI GCGC 2 cut(s) 296, 520
GsuI CTGGAG 1 cut(s) 598
HaeIII GGCC 6 cut(s) 167, 212, 233, 425, 431, 534
HhaI GCGC 2 cut(s) 297, 521
Hin1II CATG 4 cut(s) 199, 229, 313, 478
Hin6I GCGC 2 cut(s) 295, 519
HinP1I GCGC 2 cut(s) 295, 519
HinfI GANTC 1 cut(s) 144
HphI GGTGA 2 cut(s) 259, 385
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 4 cut(s) 93, 143, 448, 581
Hpy188III TCNNGA 2 cut(s) 118, 202
Hpy8I GTNNAC 1 cut(s) 219
HpyAV CCTTC 2 cut(s) 202, 223
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 286, 398, 471, 492, 558
HpyF10VI GCNNNNNNNGC 9 cut(s) 60, 63, 292, 386, 395, 404, 419, 428, 448
HpyF3I CTNAG 4 cut(s) 177, 343, 361, 536
Hsp92II CATG 4 cut(s) 199, 229, 313, 478
HspAI GCGC 2 cut(s) 295, 519
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 5 cut(s) 84, 88, 189, 367, 465
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 3 cut(s) 404, 448, 617
LpnPI CCDG 6 cut(s) 15, 42, 54, 164, 356, 601
MalI GATC 5 cut(s) 86, 90, 191, 369, 467
MboI GATC 5 cut(s) 84, 88, 189, 367, 465
MboII GAAGA 6 cut(s) 149, 184, 217, 322, 475, 568
MflI RGATCY 2 cut(s) 189, 465
MlsI TGGCCA 2 cut(s) 167, 431
MluCI AATT 1 cut(s) 510
MluNI TGGCCA 2 cut(s) 167, 431
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 6 cut(s) 93, 114, 311, 499, 545, 557
Mox20I TGGCCA 2 cut(s) 167, 431
MscI TGGCCA 2 cut(s) 167, 431
MslI CAYNNNNRTG 1 cut(s) 224
Msp20I TGGCCA 2 cut(s) 167, 431
MspA1I CMGCKG 2 cut(s) 152, 365
MspR9I CCNGG 2 cut(s) 30, 616
MvaI CCWGG 2 cut(s) 30, 616
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 9 cut(s) 60, 63, 292, 386, 395, 404, 419, 428, 448
NdeI CATATG 1 cut(s) 382
NdeII GATC 5 cut(s) 84, 88, 189, 367, 465
NlaIII CATG 4 cut(s) 199, 229, 313, 478
NspI RCATGY 1 cut(s) 478
PciI ACATGT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 162
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
PscI ACATGT 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 28, 614
PspGI CCWGG 2 cut(s) 28, 614
PspPI GGNCC 1 cut(s) 232
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PsuI RGATCY 2 cut(s) 189, 465
PvuI CGATCG 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 162
Sau3AI GATC 5 cut(s) 84, 88, 189, 367, 465
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 30, 616
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 1 cut(s) 510
SsiI CCGC 4 cut(s) 150, 410, 422, 521
StyD4I CCNGG 2 cut(s) 28, 614
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 417
TaqI TCGA 1 cut(s) 87
TasI AATT 1 cut(s) 510
TauI GCSGC 3 cut(s) 152, 413, 425
TscAI CASTG 3 cut(s) 48, 175, 297
TspDTI ATGAA 3 cut(s) 184, 242, 476
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 3 cut(s) 48, 175, 297
XceI RCATGY 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.