MD10G1336500.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014118
Physical Location & Seq
Reverse (-)
41294306 .. 41296352
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1336500.v1.1.491

Sequence Viewer

Length: 615 bp
ATGAGCTACTTAGCTGTTGGTGTGAGTCCTGGAAATGTTCCGGTGTATCACAGCACCAATTTAAAAGTGAATGATCGGAGGTTGAGGGTGGCAGAGTTGGTTTTGAGGTGCTTGATCTGCGGTCTAGGAGTTGTTGCTGCTGCTCTTGTGGGAACTGATACTCAGGTCAAAGAGATCTTCACCGTCCAGAAGAAAGCTAAATTTACAGACATGAAAGCTCTTGTGTTTTTGGTGGTGGCGAATGGGATAGTTTCTGCCTACTCTTTGGTGCAAGTGGTGCGTTGTGTTGTGAGCATGGTTAGGGGCAATGTGCTCTTCAGCAAGCCCATCGCTTGGATCATTTTCTCTGGGGATCAGGTGATGGCATATGTGTGTGTGGCAGCAGTAGGAGCTGCCACACAGTCGGCGGTGTTGGCCAAGTTGGGGCAGCCAGAGCTGCAATGGATGAAGATATGCAATATGTATGACAAGTTCTGCAACCAAGTTGGGGAGGGAATTGCCAGCGCTTTATTAGTCAGTCTAAGCACAGTCCTGCTCTCTTGTGTTTCTGCTTTCAATCTCTTCCGCTTGTACGGTGGCAACAAAAACACGACCACCGTCGCCAGTAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

205

Amino Acids

21.96

Weight (kDa)

9.46

Isoelectric Point (pI)

24.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 26 - 173 3.2e-47 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 333, 609
AciI CCGC 3 cut(s) 120, 407, 565
AclWI GGATC 2 cut(s) 344, 360
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 301
AfaI GTAC 1 cut(s) 572
AfeI AGCGCT 1 cut(s) 505
AfiI CCNNNNNNNGG 4 cut(s) 333, 423, 487, 609
AgsI TTSAA 1 cut(s) 556
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 6 cut(s) 6, 14, 197, 218, 392, 436
AluI AGCT 6 cut(s) 6, 14, 197, 218, 392, 436
Alw21I GWGCWC 1 cut(s) 315
AlwI GGATC 2 cut(s) 344, 360
Aor51HI AGCGCT 1 cut(s) 505
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 6 cut(s) 137, 140, 380, 392, 427, 436
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 172, 370
BaeI ACNNNNGTAYC 2 cut(s) 150, 183
BalI TGGCCA 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 6 cut(s) 124, 127, 379, 392, 423, 439
BccI CCATC 2 cut(s) 335, 355
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 1 cut(s) 30
BfaI CTAG 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 507
BglII AGATCT 1 cut(s) 174
BisI GCNGC 6 cut(s) 138, 141, 381, 393, 428, 437
BlsI GCNGC 6 cut(s) 139, 142, 382, 394, 429, 438
Bme1390I CCNGG 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 30
BoxI GACNNNNGTC 1 cut(s) 596
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 423, 487, 609
Bse1I ACTGG 1 cut(s) 603
Bse3DI GCAATG 2 cut(s) 313, 446
BseBI CCWGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 450
BseLI CCNNNNNNNGG 4 cut(s) 333, 423, 487, 609
BseMI GCAATG 2 cut(s) 313, 446
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 603
BseXI GCAGC 6 cut(s) 124, 127, 379, 392, 423, 439
BshFI GGCC 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 4 cut(s) 333, 423, 487, 609
BsnI GGCC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 5 cut(s) 73, 114, 174, 336, 352
BspACI CCGC 3 cut(s) 120, 407, 565
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 1 cut(s) 175
BspPI GGATC 2 cut(s) 344, 360
BspQI GCTCTTC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 313, 446
BsrI ACTGG 1 cut(s) 603
BssMI GATC 5 cut(s) 73, 114, 174, 336, 352
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 5 cut(s) 184, 402, 529, 575, 598
Bst6I CTCTTC 2 cut(s) 320, 566
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 323, 502
BstDEI CTNAG 3 cut(s) 10, 162, 521
BstF5I GGATG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 5 cut(s) 76, 117, 177, 339, 355
BstMBI GATC 5 cut(s) 73, 114, 174, 336, 352
BstMWI GCNNNNNNNGC 6 cut(s) 117, 277, 389, 413, 433, 436
BstNI CCWGG 1 cut(s) 30
BstPAI GACNNNNGTC 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 28
BstV1I GCAGC 6 cut(s) 124, 127, 379, 392, 423, 439
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 1 cut(s) 416
BtgZI GCGATG 1 cut(s) 313
BtsCI GGATG 1 cut(s) 450
Cac8I GCNNGC 2 cut(s) 323, 502
CfoI GCGC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 571
CviAII CATG 2 cut(s) 211, 295
CviJI RGCY 9 cut(s) 6, 14, 197, 218, 325, 392, 416, 430, 436
CviKI_1 RGCY 9 cut(s) 6, 14, 197, 218, 325, 392, 416, 430, 436
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 3 cut(s) 10, 162, 521
DpnI GATC 5 cut(s) 75, 116, 176, 338, 354
DpnII GATC 5 cut(s) 73, 114, 174, 336, 352
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 320, 566
EarI CTCTTC 2 cut(s) 320, 566
Eco47III AGCGCT 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 28
FaeI CATG 2 cut(s) 214, 298
FaiI YATR 7 cut(s) 212, 296, 367, 369, 454, 461, 465
FatI CATG 2 cut(s) 210, 294
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 138, 141, 381, 393, 428, 437
FokI GGATG 1 cut(s) 457
Fsp4HI GCNGC 6 cut(s) 138, 141, 381, 393, 428, 437
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 505
GluI GCNGC 6 cut(s) 138, 141, 381, 393, 428, 437
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 506
Hin1II CATG 2 cut(s) 214, 298
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HinfI GANTC 1 cut(s) 25
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 172, 370
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 602
HpyCH4III ACNGT 5 cut(s) 184, 402, 529, 575, 598
HpyCH4V TGCA 4 cut(s) 271, 439, 456, 477
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 277, 389, 413, 433, 436
HpyF3I CTNAG 3 cut(s) 10, 162, 521
Hsp92II CATG 2 cut(s) 214, 298
HspAI GCGC 1 cut(s) 504
Kzo9I GATC 5 cut(s) 73, 114, 174, 336, 352
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 389
Lsp1109I GCAGC 6 cut(s) 124, 127, 379, 392, 423, 439
MaeI CTAG 1 cut(s) 125
MalI GATC 5 cut(s) 75, 116, 176, 338, 354
MboI GATC 5 cut(s) 73, 114, 174, 336, 352
MboII GAAGA 5 cut(s) 169, 202, 307, 460, 553
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 3 cut(s) 58, 200, 495
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 1 cut(s) 34
MnlI CCTC 4 cut(s) 72, 78, 99, 484
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 6 cut(s) 117, 277, 389, 413, 433, 436
NdeI CATATG 1 cut(s) 367
NdeII GATC 5 cut(s) 73, 114, 174, 336, 352
NlaIII CATG 2 cut(s) 214, 298
PciSI GCTCTTC 1 cut(s) 320
PflMI CCANNNNNTGG 2 cut(s) 333, 609
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 6 cut(s) 139, 142, 382, 394, 429, 438
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PshAI GACNNNNGTC 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 6 cut(s) 138, 141, 381, 393, 428, 437
Sau3AI GATC 5 cut(s) 73, 114, 174, 336, 352
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 370
Sse9I AATT 3 cut(s) 58, 200, 495
SsiI CCGC 3 cut(s) 120, 407, 565
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 5 cut(s) 184, 402, 529, 575, 598
TasI AATT 3 cut(s) 58, 200, 495
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseI GCWGC 6 cut(s) 137, 140, 380, 392, 427, 436
TspDTI ATGAA 2 cut(s) 227, 461
Van91I CCANNNNNTGG 2 cut(s) 333, 609
XapI RAATTY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.