Rroxscaffold_1G00075440

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014118
Physical Location & Seq
Reverse (-)
96187463 .. 96188822
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075440.1

Sequence Viewer

Length: 618 bp
ATGAGCTACTTGGGTGTGGGTGTTAGTCCTGGGAATGTCCCAGTGTCTCACAGCAGCAGCACTACAAATCTAAAAGTGATCGATCGGAGAGTGAGGGTGGCAGAGTTGGTCTTGAGGTTTCTCATCTGTGGTCTCGGACTCGCCGCTGCTCTTCTTGTGGCCACTGATACTCAGGTCAAAGAGATCTTCATGATCAAGAAGAAGGCCAAGTTCACGGACATGAAGGCCCTTGTGTTTTTGGTAATAGCCAATGGAATAGCTGCTGCTTACTCTTTGCTGCAAGCACTGCGCTGTGTTATGAGCATGGTTAGAGGAAATGTTCTCTTCAGCAAGCCCTTAGCTTGGATTATATTCTCAGCTGATCAGGTGATAGCATATGTGGCTGTGGCTGCAATAGGAGCTGCGGCACAGTCTGCGGCCTTGGCCAAGTTGGGGCAGTCGGAGCTACAATGGATGAAGATCTGCAACATGTATGACAAGTTCTGCAACCAAGTTGGGGAGGGAATTGCTAGCGCGGTGTTAGTTGGCCTCAGCACAATCCTCATCTCTTGCATTTCTGCTTTCAATCTCTTCCGATTGTATGGTGGCAACAAGAACAAGAGCAGCTCCAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

205

Amino Acids

21.98

Weight (kDa)

9.77

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 29 - 174 2.6e-46 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014118)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35760
fragaria_vesca FvH4_3g00580
malus_domestica MD00G1058300.v1.1 MD10G1336500.v1.1
prunus_persica Prupe.4G007000_v2.0.a1
pyrus_communis pycom05g32590
rosa_chinensis RchiOBHm_Chr5g0000791
rosa_laevigata RLG00000030896
rosa_multiflora Rmu_sc0011992.1_g000001 Rmu_sc0040466.1_g000001
rosa_roxburghii Rroxscaffold_1G00075440
rosa_rugosa Rorug04G0386600
rosa_samantha Rh5AG006500 Rh5BG008400 Rh5CG007100 Rh5DG007100
rosa_wichuraiana Rw5G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 515
AciI CCGC 4 cut(s) 144, 404, 416, 515
AcoI YGGCCR 2 cut(s) 159, 423
AcuI CTGAAG 1 cut(s) 310
AfiI CCNNNNNNNGG 3 cut(s) 342, 432, 496
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 1 cut(s) 565
AjnI CCWGG 2 cut(s) 28, 608
AluBI AGCT 7 cut(s) 6, 260, 341, 359, 401, 445, 606
AluI AGCT 7 cut(s) 6, 260, 341, 359, 401, 445, 606
Alw26I GTCTC 2 cut(s) 51, 137
AoxI GGCC 6 cut(s) 159, 204, 225, 417, 423, 526
ApeKI GCWGC 9 cut(s) 54, 57, 146, 260, 263, 277, 389, 401, 603
AspLEI GCGC 2 cut(s) 291, 515
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 379
AsuNHI GCTAGC 1 cut(s) 509
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 2 cut(s) 161, 425
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 8 cut(s) 66, 69, 133, 247, 250, 264, 376, 388
BciT130I CCWGG 2 cut(s) 30, 610
BclI TGATCA 2 cut(s) 192, 361
BcoDI GTCTC 2 cut(s) 51, 137
BfaI CTAG 1 cut(s) 510
BglII AGATCT 2 cut(s) 183, 459
Bme1390I CCNGG 2 cut(s) 30, 610
BmgT120I GGNCC 1 cut(s) 226
BmrFI CCNGG 2 cut(s) 30, 610
BmrI ACTGGG 1 cut(s) 35
BmtI GCTAGC 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 592
Bpu10I CCTNAGC 2 cut(s) 337, 530
BpuEI CTTGAG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 29, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 432, 496
Bse1I ACTGG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 458
BseBI CCWGG 2 cut(s) 30, 610
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 29, 420
BseGI GGATG 1 cut(s) 459
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 3 cut(s) 342, 432, 496
BseMII CTCAG 3 cut(s) 185, 369, 544
BseNI ACTGG 1 cut(s) 41
BseXI GCAGC 8 cut(s) 66, 69, 133, 247, 250, 264, 376, 388
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 342, 432, 496
BsmAI GTCTC 2 cut(s) 51, 137
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
Bso31I GGTCTC 1 cut(s) 137
Bsp143I GATC 6 cut(s) 78, 82, 183, 192, 361, 459
BspACI CCGC 4 cut(s) 144, 404, 416, 515
BspANI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BspCNI CTCAG 3 cut(s) 184, 368, 543
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 189
BspOI GCTAGC 1 cut(s) 513
BspQI GCTCTTC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 29, 420
BssMI GATC 6 cut(s) 78, 82, 183, 192, 361, 459
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 30, 610
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 3 cut(s) 156, 329, 575
BstAPI GCANNNNNTGC 2 cut(s) 286, 413
BstC8I GCNNGC 3 cut(s) 282, 332, 511
BstDEI CTNAG 4 cut(s) 171, 337, 355, 530
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 2 cut(s) 291, 515
BstKTI GATC 6 cut(s) 81, 85, 186, 195, 364, 462
BstMAI GTCTC 2 cut(s) 51, 137
BstMBI GATC 6 cut(s) 78, 82, 183, 192, 361, 459
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
BstNI CCWGG 2 cut(s) 30, 610
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 2 cut(s) 28, 608
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 8 cut(s) 66, 69, 133, 247, 250, 264, 376, 388
BstX2I RGATCY 2 cut(s) 183, 459
BstYI RGATCY 2 cut(s) 183, 459
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 3 cut(s) 48, 162, 284
Cac8I GCNNGC 3 cut(s) 282, 332, 511
CciI TCATGA 1 cut(s) 189
CfoI GCGC 2 cut(s) 291, 515
Cfr13I GGNCC 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 81
CviAII CATG 4 cut(s) 190, 220, 304, 469
DdeI CTNAG 4 cut(s) 171, 337, 355, 530
DpnI GATC 6 cut(s) 80, 84, 185, 194, 363, 461
DpnII GATC 6 cut(s) 78, 82, 183, 192, 361, 459
EaeI YGGCCR 2 cut(s) 159, 423
Eam1104I CTCTTC 3 cut(s) 156, 329, 575
EarI CTCTTC 3 cut(s) 156, 329, 575
Eco130I CCWWGG 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 28, 608
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 4 cut(s) 193, 223, 307, 472
FaqI GGGAC 1 cut(s) 23
FatI CATG 4 cut(s) 189, 219, 303, 468
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 2 cut(s) 192, 361
FokI GGATG 1 cut(s) 466
FspBI CTAG 1 cut(s) 510
GlaI GCGC 2 cut(s) 290, 514
GsuI CTGGAG 1 cut(s) 592
HaeIII GGCC 6 cut(s) 161, 206, 227, 419, 425, 528
HhaI GCGC 2 cut(s) 291, 515
Hin1II CATG 4 cut(s) 193, 223, 307, 472
Hin6I GCGC 2 cut(s) 289, 513
HinP1I GCGC 2 cut(s) 289, 513
HinfI GANTC 1 cut(s) 138
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 4 cut(s) 87, 137, 442, 575
Hpy188III TCNNGA 3 cut(s) 112, 190, 196
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 2 cut(s) 196, 217
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 280, 392, 465, 486, 552
HpyF10VI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
HpyF3I CTNAG 4 cut(s) 171, 337, 355, 530
Hsp92II CATG 4 cut(s) 193, 223, 307, 472
HspAI GCGC 2 cut(s) 289, 513
Ksp22I TGATCA 2 cut(s) 192, 361
Kzo9I GATC 6 cut(s) 78, 82, 183, 192, 361, 459
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 3 cut(s) 398, 442, 611
LpnPI CCDG 6 cut(s) 15, 42, 54, 158, 350, 595
Lsp1109I GCAGC 8 cut(s) 66, 69, 133, 247, 250, 264, 376, 388
MaeI CTAG 1 cut(s) 510
MalI GATC 6 cut(s) 80, 84, 185, 194, 363, 461
MboI GATC 6 cut(s) 78, 82, 183, 192, 361, 459
MboII GAAGA 6 cut(s) 143, 178, 211, 316, 469, 562
MflI RGATCY 2 cut(s) 183, 459
MlsI TGGCCA 2 cut(s) 161, 425
MluCI AATT 1 cut(s) 504
MluNI TGGCCA 2 cut(s) 161, 425
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 6 cut(s) 87, 108, 305, 493, 539, 551
Mox20I TGGCCA 2 cut(s) 161, 425
MscI TGGCCA 2 cut(s) 161, 425
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 2 cut(s) 161, 425
MspA1I CMGCKG 2 cut(s) 146, 359
MspR9I CCNGG 2 cut(s) 30, 610
MvaI CCWGG 2 cut(s) 30, 610
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 7 cut(s) 286, 380, 389, 398, 413, 422, 442
NdeI CATATG 1 cut(s) 376
NdeII GATC 6 cut(s) 78, 82, 183, 192, 361, 459
NheI GCTAGC 1 cut(s) 509
NlaIII CATG 4 cut(s) 193, 223, 307, 472
NspI RCATGY 1 cut(s) 472
PagI TCATGA 1 cut(s) 189
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 156
Ple19I CGATCG 1 cut(s) 85
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 28, 608
PspGI CCWGG 2 cut(s) 28, 608
PspPI GGNCC 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PsuI RGATCY 2 cut(s) 183, 459
PvuI CGATCG 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 156
Sau3AI GATC 6 cut(s) 78, 82, 183, 192, 361, 459
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 30, 610
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 1 cut(s) 504
SsiI CCGC 4 cut(s) 144, 404, 416, 515
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 28, 608
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 411
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 504
TauI GCSGC 3 cut(s) 146, 407, 419
TscAI CASTG 3 cut(s) 48, 169, 291
TseI GCWGC 9 cut(s) 54, 57, 146, 260, 263, 277, 389, 401, 603
TspDTI ATGAA 3 cut(s) 178, 236, 470
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 3 cut(s) 48, 169, 291
XceI RCATGY 1 cut(s) 472
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.